Culture of animal cells: a manual of basic technique
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, NJ
Wiley-Liss
2005
|
| Ausgabe: | 5. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zusatz zum Sachtitel lautet ab der 6. Aufl.: A manual of basic technique and specialized applications |
| Beschreibung: | XXVI, 642 S. Ill., graph. Darst. |
| ISBN: | 0471453293 9780471453291 |
Internformat
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| 100 | 1 | |a Freshney, Robert Ian |d 1938-2019 |e Verfasser |0 (DE-588)1055981578 |4 aut | |
| 245 | 1 | 0 | |a Culture of animal cells |b a manual of basic technique |c R. Ian Freshney |
| 250 | |a 5. ed. | ||
| 264 | 1 | |a Hoboken, NJ |b Wiley-Liss |c 2005 | |
| 300 | |a XXVI, 642 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zusatz zum Sachtitel lautet ab der 6. Aufl.: A manual of basic technique and specialized applications | ||
| 650 | 7 | |a Cell Cultures |2 cabt | |
| 650 | 4 | |a Cell culture |v Laboratory manuals | |
| 650 | 4 | |a Tissue culture |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1805083683773743104 |
|---|---|
| adam_text |
List of
List of Color Plates,
Preface,
Abbreviations,
1.
1.1.
1.2.
1.2.1.
1.2.2.
Sample,
1.2.3.
1.2.4.
1.3.
1.3.1.
1.3.2.
1.3.3.
1.3.4.
1.3.5.
1.4.
1.5.
Exercise
Use,
Exercise
Exercise
Exercise
Exercise
Aseptic Technique H: Preparing Medium for
l
2.1.
2.2.
Exertise
of Fluids,
Exercise
Feeding a Monolayer Culture,
Washing and Sterilizing Glassware,
Preparation and Sterilization of Water,
Preparation and Sterilization of Dulbeao's
Phosphate-Buffered Saline (D-PBS) without Ca2+ and
M/+ (D-PBSA),
Exercise
Exercise
and Sterilization by Filtration,
Exercise
Wx Stock,
Exerdse
Powder,
Exercise
Electronic Counter,
Exercise
Suspension,
Exercise
Growing in Monolayer,
Exercise
Giemsa,
Exercise
Curve,
2.3.
Exercise
Exercise
Exercise
Exercise
Exercise
2.4.
VII
VIH
CONTENTS
3.
3.1.
3.2.
3.2.1.
3.2.2.
3.2.3.
3.2.4.
3.2.5.
3.3.
3.3.1.
3.3.2.
3.4.
3.4.1.
3.4.2.
3.5.
3.6.
3.7.
3.8.
3.8.1.
3.9.
3.10.
4.
4.1.
4.2.
4.3.
4.3.1.
4.3.2.
4.3.3.
4.3.4.
4.4.
4.4.1.
4.4.2.
4.5.
4.5.1.
4.5.2.
4.5.3.
5.
5.1.
Laboratory,
5.2.
5.2.1.
5.2.2.
5.2.3.
5.2.4.
5.2.5.
5.2.6.
5.2.7.
Dispensing,
5.2.8.
5.2.9.
5.2.10.
5.3.
5.3.1.
5.3.2.
5.3.3.
5.3.4.
5.3.5.
5.4.
5.4.1.
5.4.2.
5.4.3.
5.4.4.
5.4.5.
5.4.6. pH
5.4.7.
5.4.8.
5.4.9.
5.4.10.
5.4.11.
5.5.
5.5.1.
5.5.2.
5.5.3.
5.6.
5.6.1.
5.6.2.
5.6.3.
5.6.4.
5.6.5.
5.7.
5.7.1.
5.7.2.
5.7.3.
5.7.4.
5.8.
5.8.1.
5.8.2.
5.8.3.
5.8.4.
5.8.5.
5.8.6.
5.8.7.
5.8.8.
6.
6.1.
6.1.1.
6.2.
6.2.1.
6.2.2.
6.2.3.
6.2.4.
6.2.5.
CONTENTS
IX
6.3. Sterile
6.3.1.
6.3.2.
6.3.3.
6.3.4.
6.3.5.
6.3.6.
6.4.
6.5.
Protocol
Flow,
Protocol
6.5.1.
Protocol
6.6.
6.6.1.
6.6.2.
6.6.3.
7.
7.1.
7.2.
7.3.
7.4.
7.5.
7.5.1.
7.5.2.
7.5.3.
7.5.4.
7.5.5.
7.5.6.
7.5.7.
7.6.
7.7.
7.7.1. Ingestion, 94
7.7.2.
7.7.3.
7.7
Sources,
7.8.
7.8.1.
7.8.2.
7.8.3.
7.8.4.
7.8.5.
7.8.6.
7.9.
7.9.1.
7.9.2.
7.10.
7.10.1.
7.10.2.
7.10.3.
8.
8.1.
8.1.1.
8.1.2.
8.2.
8.2.1.
8.2.2.
8.2.3.
8.2.4.
8.2.5.
8.2.6.
8.3.
8.3.1.
8.4.
8.4.1.
Protocol
8.4.2.
8.4.3.
8.4.4.
8.4.5.
9.
9.1.
9.2.
9.2.1. pH, 115
Protocol
9.2.2.
9.2.3.
9.2.4.
9.2.5.
9.2.6.
9.2.7.
9.2.8.
9.3.
9.4.
9.4.1.
9.4.2.
9.4.3.
9.4.4.
9.4.5.
9.4.6.
9.4.7.
9.5.
9.5.1.
9.5.2.
9.5.3.
9.5.4.
9.5.5.
9.5.6.
9.5.7.
9.6.
9.6.1.
CONTENTS
9.6.2.
9.6.3.
9.7.
9.7.1.
9.7.2.
9.7.3.
Serum-Free Media,
Disadvantages of Serum,
Advantages of Serum-Free Media,
10.2.1.
10.2.2.
Differentiation,
,
.
10.4.1.
10.4.2.
10.4.3.
10.4.4.
10.4.5.
10.4.6.
.
10.5.1.
Media,
10.5.2.
10.5.3.
.
.
.
.
10.1
10.2
10.3
10.4
10.5
10.6
10.7
10.8
10.9
11.
11.1.
11.2.
11.3.
11.3.1.
Protocol ii.l. Preparation and Sterilization of
Glassware,
11.3.2.
Protocol
Pipettes,
11.3.3.
Protocol
Caps,
11.3.4.
11.3.5.
11.3.6.
Protocol
11.4.
11.4.1.
Protocol
Water (UPW),
11.4.2.
Protocol
D-PBSA,
11.4.3.
Media,
Protocol
Stock,
Protocol
Concentrate,
11.4.4.
Protocol
11.4.5.
Protocol
Medium,
11.5.
11.5.1.
11.5.2.
Protocol
Filter,
Protocol
Filter Flask,
Protocol
In-Line Filter,
Protocol
Filter,
11.5.3.
Protocol
of Serum,
Protocol
11.5.4.
Reagents,
11.6.
11.6.1.
11.6.2.
11.6.3.
11.6.4.
12.
12.1.
12.2.
12.2.1.
Protocol
12.2.2.
Protocol
12.2.3.
Protocol
12.3.
12.3.1.
Protocol
12.3.2.
12.3.3.
Protocol
Warm
12.3.4.
Preexposure,
CONTENTS
XI
Protocol
Cold
12.3.5.
Protocol
12.3.6.
12.3.7. Collagenase, 192
Protocol
Collagenase, 192
12.3.8.
Protocol
Sieving,
12.3.9.
Cells,
Protocol
12.3.10.
12.3.11.
13.
13.1.
13.2.
13.3.
13.4.
13.5.
13.6.
13.6.1.
13.6.2.
Protocol
13.7.
13.7.1.
Protocol
13.7.2.
13.7.3.
13.7.4.
13.7.5.
Suspension,
Protocol
13.7.6.
Conditions,
13.7.7.
13.7.8.
14.
14.1.
Protocol
14.2.
14.2.1.
Growth,
14.2.2.
Protocol
14.2.3.
Protocol
14.3.
Protocol
Protocol
14.4.
Protocol
Cloning Rings,
Protocol
Irradiation,
14.4.1.
Clones,
14.4.2.
Protocol
14.5.
14.6.
14.7.
Protocol
Amplification,
14.8.
14.8.1.
14.8.2.
14.8.3.
14.8.4.
14.8.5.
15.
15.1.
Protocol
Density Gradient,
15.2.
15.2.1.
15.2.2.
15.3.
15.3.1.
15.3.2.
Protocol
(MACS),
15.4.
15.5.
15.6.
Separation,
IB. Characterization,
16.1.
16.2.
16.3.
16.3.1.
16.3.2.
16.3.3.
16.3.4.
16.4.
16.4.1.
Protocol
16.4.2.
Protocol
Protocol
XII
CONTENTS
16.4.3.
Cultures,
16.4.4.
Cytology,
Protocol
Cytology by Cytocentrifuge,
Protocol
16.4.5.
Protocol
a Microscope,
16.5.
Protocol
16.5.1.
16.5.2.
16.5.3.
16.6. DNA
16.6.1. DNA
16.6.2. DNA
Protocol
Cell Lines,
16.6.3. DNA
Protocol
Cell Lines,
16.7.
16.8.
16.8.1.
16.8.2.
Authentikit,
Protocol
16.9.
16.9.1.
Protocol
16.9.2.
16.10.
17.
17.1.
17.1.1.
17.1.2.
17.2.
17.3.
17.4.
17.5.
17.6.
17.7.
17.7.1.
17.7.2.
Factors,
17.7.3.
17.7.4.
17.7.5.
17.8.
17.9.
18.
18.1.
18.2.
18.3.
18.3.1.
18.3.2.
18.4.
18.4.1.
18.4.2.
18.4.3.
Fibroblasts,
Protocol
18.4.4.
Immortalization,
Protocol
Stem Cells by Telomerase,
18.4.5.
18.5.
18.5.1.
18.5.2.
Protocol
Proliferation,
18.5.3.
18.5.4.
18.6.
18.6.1.
18.6.2.
18.6.3.
18.6.4.
18.6.5.
19.
19.1.
19.1.1.
19.1.2.
19.1.3.
Hood,
19.1.4.
Protocol
19.1.5.
19.1.6.
19.1.7.
19.1.8.
19.2.
19.3.
19.3.1.
19.3.2.
19.3.3.
Mycoplasma,
Protocol
of Mycoplasma,
19.3.4.
Protocol
CONTENTS
XIII
19.3.5.
Mycoplasma,
19.3.6.
19.4.
19.4.1.
Protocol
Contamination,
19.4.2.
19.4.3.
19.4.4.
19.5.
19.6.
20.
20.1.
20.2.
Cryopreservation,
20.3.
20.3.1.
Freezing,
20.3.2.
20.3.3.
20.3.4.
20.3.5.
Protocol
20.3.6.
20.3.7.
Protocol
20.4.
20.4.1.
20.4.2.
20.5.
20.6.
20.6.1.
20.6.2.
21.
21.1.
21.1.1.
Protocol
21.1.2.
Protocol
Electrical Resistance,
21.1.3.
21.2.
21.3. DNA
Protocol
Hoechst 33258, 341
21.4.
21.4.1.
Protocol
Bradford Method,
21.5.
21.5.1. DNA
Protocol
f
21.5.2.
Protocol
21.6.
Immunoassay,
21.7.
21.7.1.
21.7.2.
21.8.
21.8.1.
21.8.2.
21.9.
21.9.1.
21.9.2.
Protocol
in Flasks,
Protocol
Multiwell Plates,
21.9.3.
Curves,
21.9.4.
and Cell Density,
21.9.5.
Protocol
in Suspension,
21.9.6.
21.9.7.
21.10.
Protocol
21.10.1.
21.10.2.
21.11.
Protocol
f
21.11.1.
Protocol
21.11.2.
21.11.3.
21.12.
21.13.
22.
22.1.
22.2.
22.3.
22.3.1.
Protocol
Exclusion,
Protocol
Dye Uptake,
XIV
CONTENTS
22.3.2.
Protocol
22.3.3.
22.3.4.
22.3.5. Microtitration
Protocol
22.3.6.
Clonogenic Survival,
22.3.7.
22.4.
22.4.1.
22.4.2.
22.4.3.
22.5.
22.5.1.
Exchange,
Protocol
22.5.2.
22.6.
23.
23.1.
23.2.
23.2.1.
Protocol
23.2.2.
Protocol
23.2.3.
Protocol
23.2.4.
Protocol
23.2.5.
Protocol
Colonie
23.2.6.
Protocol
23.2.7.
Protocol
23.2.8.
Protocol
23.2.9.
Protocol
23.2.10.
Protocol
23.2.11.
Protocol
23.3.
23.3.1.
23.3.2.
Protocol
23.3.3.
Protocol
Muscle Cells,
Protocol
Skeletal Muscle,
Protocol
Skeletal Muscle,
23.3.4.
Protocol
23.3.5.
Protocol
23.3.6.
Protocol
Endothelial Cells,
23.4.
23.4.1.
Protocol
23.4.2.
Protocol
23.4.3.
23.4.4.
Protocol
23.4.5.
Identity,
23.5.
23.5.1.
Protocol
from Bone Marrow,
23.5.2.
Assays,
Protocol
Forming Assays, Ail
23.6.
23.7.
23.7.1.
23.7.2. Multipotent
Adult,
23.7.3.
Zł.
24.1.
24.2.
24.3.
24.4.
24.5.
24.6.
24.6.1.
24.7.
24.7.1.
24.7.2.
Protocol
Layers,
24.7.3.
24.7.4.
24.7.5.
24.7.6.
Cultures,
CONTENTS
XV
24.7.7.
Protocol
24.8.
24.8.1.
24.8.2.
24.8.3.
24.8.4.
Protocol
24.8.5.
24.8.6.
24.8.7.
24.8.8.
24.8.9.
24.8.10.
24.8.11.
24.8.12.
24.8.13.
24.8.14. Lymphoma
25.
25.1.
Expression,
25.1.1.
25.1.2.
25.1.3.
25.2.
25.2.1.
25.2.2.
25.2.3.
25.2.4.
25.2.5.
Protocol
25.3.
25.3.1.
25.3.2.
25.3.3.
Protocol
25.3
25.3.5.
Alginate,
Protocol
25.3.6. FÜter
Protocol
25.3.7.
Protocol
25.3.8.
Engineering,
25.4.
26.
26.1.
Protocol
Suspension Culture,
26.1.1.
26.1.2.
26.1.3.
26.2.
26.2.1.
Protocol
26.2.2.
26.2.3.
Protocol
26.2.4.
Protocol
26.2.5.
26.2.6.
26.3.
27.
27.1.
Protocol
27.1.1.
Protocol
27.2.
Protocol
27.3.
Protocol
27.4.
27.5.
27.5.1.
27.5.2.
27.6.
Protocol
27.7.
27.7.1.
Protocol
Zebrafish Embryos,
27.7.2.
Protocol
27.8.
27.8.1.
Situ Hybridization,
Protocol
Hybridization,
27.8.2.
Analysis of Genes and Chromosomes,
Protocol
and Chromosome Painting,
27.9.
Protocol
27.9.1.
27.10.
Protocol
Monoclonal Antibodies,
XVI
CONTENTS
27.11. DNA Transfer, 496
27.11.1.
Phosphate, 496
27.11.2. Lipofection, 496
Protocol
Lipofection, 497
27.11.3. Electroporation, 498
Protocol
Electroporation, 498
27.11.4.
27.11.5. Reporter Genes, 500
Protocol
ß-Galactosidase, 500
Protocol
(CAT) Assay,
28.
28.1
Problem
28.2.
28.3.
28.4.
28.5.
28.6.
28.7.
28.8.
Slow Cell Growth,
28.1.1.
Stock,
28.1.2.
People are Having Difficulty,
28.1.3.
Laboratory?,
MEDIUM,
28.2.1.
28.2.2.
Purity of Constituents,
28.3.1.
Correctly?,
28.3.2.
28.3.3.
28.3.4.
Plastics,
Glassware,
28.5.1.
Microbial Contamination,
28.6.1.
28.6.2.
28.6.3.
28.6.4.
Chemical Contamination,
28.7.1.
28.7.2.
28.7.3.
28.7.4.
28.7.5.
Primary Culture,
28.8.1.
28.8.2.
28.8.3.
28.9.
28.9.1.
28.9.2.
28.10.
28.10.1.
28.10.2.
28.11.
28.11.1.
28.11.2.
28.11.3.
28.11.4.
28.12.
28.12.1.
28.12.2.
28.12.3.
28.12.4.
28.12.5.
28.13.
28.13.1.
28.13.2.
28.13.3.
28.14.
28.14.1.
28.14.2.
Cryopreservation,
28.14.3.
28.14.4.
28.15.
28.16.
28.16.1.
28.16.2.
Resistance,
28.17.
28.17.1.
28.17.2.
28.17.3.
29.
Appendix I: Preparation of Reagents,
Appendix II: Sources of Equipment and
Materials,
Appendix III: Suppliers and Other Resources,
Appendix IV: Glossary,
Appendix V: General Textbooks and Relevant
Journals,
References,
Index, |
| any_adam_object | 1 |
| author | Freshney, Robert Ian 1938-2019 |
| author_GND | (DE-588)1055981578 |
| author_facet | Freshney, Robert Ian 1938-2019 |
| author_role | aut |
| author_sort | Freshney, Robert Ian 1938-2019 |
| author_variant | r i f ri rif |
| building | Verbundindex |
| bvnumber | BV020015393 |
| classification_rvk | WC 5100 WX 6000 WX 6600 WX 6603 |
| classification_tum | CIT 972f BIO 655f BIO 770f BIO 768f |
| ctrlnum | (OCoLC)253754266 (DE-599)BVBBV020015393 |
| dewey-full | 590.724 571.638 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 590 - Animals 571 - Physiology & related subjects |
| dewey-raw | 590.724 571.638 |
| dewey-search | 590.724 571.638 |
| dewey-sort | 3590.724 |
| dewey-tens | 590 - Animals 570 - Biology |
| discipline | Biologie Chemie-Ingenieurwesen Biotechnologie |
| edition | 5. ed. |
| format | Book |
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| id | DE-604.BV020015393 |
| illustrated | Illustrated |
| indexdate | 2024-07-20T07:52:27Z |
| institution | BVB |
| isbn | 0471453293 9780471453291 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-013336867 |
| oclc_num | 253754266 |
| open_access_boolean | |
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| physical | XXVI, 642 S. Ill., graph. Darst. |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Wiley-Liss |
| record_format | marc |
| spelling | Freshney, Robert Ian 1938-2019 Verfasser (DE-588)1055981578 aut Culture of animal cells a manual of basic technique R. Ian Freshney 5. ed. Hoboken, NJ Wiley-Liss 2005 XXVI, 642 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zusatz zum Sachtitel lautet ab der 6. Aufl.: A manual of basic technique and specialized applications Cell Cultures cabt Cell culture Laboratory manuals Tissue culture Laboratory manuals Zellkultur (DE-588)4067547-6 gnd rswk-swf Gewebekultur (DE-588)4157245-2 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Tiere (DE-588)4060087-7 gnd rswk-swf Tierzelle (DE-588)4185509-7 gnd rswk-swf Gewebekultur (DE-588)4157245-2 s Tierzelle (DE-588)4185509-7 s DE-604 Zellkultur (DE-588)4067547-6 s Tiere (DE-588)4060087-7 s Methode (DE-588)4038971-6 s 1\p DE-604 2\p DE-604 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013336867&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Freshney, Robert Ian 1938-2019 Culture of animal cells a manual of basic technique Cell Cultures cabt Cell culture Laboratory manuals Tissue culture Laboratory manuals Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd Methode (DE-588)4038971-6 gnd Tiere (DE-588)4060087-7 gnd Tierzelle (DE-588)4185509-7 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4157245-2 (DE-588)4038971-6 (DE-588)4060087-7 (DE-588)4185509-7 |
| title | Culture of animal cells a manual of basic technique |
| title_auth | Culture of animal cells a manual of basic technique |
| title_exact_search | Culture of animal cells a manual of basic technique |
| title_full | Culture of animal cells a manual of basic technique R. Ian Freshney |
| title_fullStr | Culture of animal cells a manual of basic technique R. Ian Freshney |
| title_full_unstemmed | Culture of animal cells a manual of basic technique R. Ian Freshney |
| title_short | Culture of animal cells |
| title_sort | culture of animal cells a manual of basic technique |
| title_sub | a manual of basic technique |
| topic | Cell Cultures cabt Cell culture Laboratory manuals Tissue culture Laboratory manuals Zellkultur (DE-588)4067547-6 gnd Gewebekultur (DE-588)4157245-2 gnd Methode (DE-588)4038971-6 gnd Tiere (DE-588)4060087-7 gnd Tierzelle (DE-588)4185509-7 gnd |
| topic_facet | Cell Cultures Cell culture Laboratory manuals Tissue culture Laboratory manuals Zellkultur Gewebekultur Methode Tiere Tierzelle |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013336867&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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