Human retrovirus protocols: virology and molecular biology
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in molecular biology
304 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Klappentext Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 494 S. Ill., graph. Darst. |
| ISBN: | 1588294951 |
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| 245 | 1 | 0 | |a Human retrovirus protocols |b virology and molecular biology |c ed. by Tuofu Zhu |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2005 | |
| 300 | |a XIV, 494 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 304 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Rétrovirus - Manuels de laboratoire | |
| 650 | 4 | |a Retroviruses |v Laboratory manuals | |
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Datensatz im Suchindex
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| adam_text | Advances in our understanding of human retroviruses have been occurring at a very rapid
pace and have contributed significantly to the development of new biotechnologies. In Human
Retrovirus
in detail the basic and state-of-the-art methods they have optimized for investigating the molecular
biology of this class of
for the isolation and detection of human retroviruses to advanced methods for exploring the interplay
between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation
and propagation of
blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are
also assays for determining the cell tropism of HIV-1
expression with HIV-1 infection by microarrays, as well as forphenotyping HIV-1 infected monocytes
and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting
CD4+, a new cloning system for making
of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1
infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each
offering step-by-step laboratory instructions, an introduction outlining the principle behind the tech¬
nique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding
known pitfalls.
State-of-the-art and highly practical, Human
Biology offers novice and experienced researchers alike proven tools essential for productive study
and understanding of the human retroviruses.
•
molecular biology of human retroviruses results
•
of human retroviruses and avoiding known pitfalls
•
propagation
Contents
Preface v
Contributors xi
Part I. Methods for Virus Isolation and Detection
1 Enhanced Culture Assay for Detection and Quantitation
of Latently Infected, Resting CD4+ T-Cells Carrying
Replication-Competent Virus in HIV-1 -Infected Individuals
Janet D. Siliciano and Robert F. Siliciano 3
2 Isolation, Propagation, and Titration of Human Immunodeficiency
Virus Type 1 From Peripheral Blood of Infected Individuals
Hanneke Schuitemaker and Neeltje A. Kootstra 17
3 Isolation of Human Immunodeficiency Virus Type 1 From Peripheral
Blood Monocytes
Paul R. Gorry, Secondo Sonza, Katherine Kedzierska,
and Suzanne M. Crowe 25
4 Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived
Macrophages and Recovery of Virus From Brain
and Cerebrospinal Fluid
Santhi Gorantla, Myhanh Che, and Howard E. Gendelman 35
5 Isolation and HIV-1 Infection of Primary Human Microglia
From Fetal and Adult Tissue
Kathleen Borgmann, Howard E. Gendelman, and Anuja Ghorpade 49
6 Isolation of Human Immunodeficiency Virus Type 1 From Semen
and Vaginal Fluids
Ann A. Kiessling 71
7 Isolation and Quantification of HIV From Lymph Nodes
Catherine Tamalet 87
8 Isolation of Human Immunodeficiency Virus Type 2 Biological
Clones From Peripheral Blood Lymphocytes
Hetty Blaak 95
9 Isolation and Confirmation of Human T-Cell Leukemia Virus
Type 2 From Peripheral Blood Mononuclear Cells
Michael D. Lairmore and Andy Montgomery /13
vii
viii Contents
10 Isolation of Foamy Viruses From Peripheral Blood Lymphocytes
Joe lle Tobaly-Tapiero, Patricia Bittoun, and AH Sai b 125
11 Alu-UR Real-Time Nested PCR Assay for Quantifying
Integrated HIV-1 DNA
Audrey Brussel, Olivier Delelis, and Pierre Sonigo 139
12 Quantification of HFV-Integrated DNA in Human Cells
by Alu-UR Real-Time PCR
Olivier Delelis, Audrey Brussel, and Pierre Sonigo 755
1 3 Detection of HIV-1 Provirus and RNA by In Situ Amplification
Alcina Nicol and Gerard J. Nuovo 171
14 Detection of HTLV-1 Gene on Cytologic Smear Slides
Kenji Kashima, Tsutomu Daa, and Shigeo Yokoyama 183
1 5 Detection of HIV-2 by PCR
Feng Gao 191
1 6 Quantitation of HIV-1 Viral RNA in Blood Plasma
and Genital Secretions
Susan A. Fiscus 201
1 7 Quantification of Proviral DNA Load of Human Immunodeficiency
Virus Type 2 Subtypes A and B Using Real-Time PCR
Marie Gueudin, Florence Damond, and Francois Simon 215
1 8 Plasma RNA Viral Load in HIV-1 Group O Infection
by Real-Time PCR
Marie Gueudin and Francois Simon 221
19 A New Combined HIV p24 Antigen and Anti-HIV-1/2/O
Screening Assay
Susanne Polywka, Hedwig Duttmann, Frank Lubben,
Rainer Laufs, and Jtirgen Feldner 229
20 A New Automated Fourth-Generation HIV Screening Assay
With Sensitive Antigen Detection Module and High Specificity
Bernard Weber 245
21 Detection of Antiviral Antibodies Using Enzyme-Linked
Immunosorbent Assay
IngridG. Winkler 257
22 Plaque-Reduction Assays for Human and Simian
Immunodeficiency Virus Neutralization
Anna Nordqvist and Eva Maria Fenyo 273
23 Detection of Drug-Resistant HIV-1 Strains
Ann A. Kiessling, S. J. Eyre, and B. D. Desmarais 287
Contents ix
Part II. Molecular Biology Methods
24 Determination of Cell Tropism of HIV-1
Neeltje A. Kootstra and Hanneke Schuitemaker 317
25 Determination of Co-Receptor Usage of HIV-1
Hanneke Schuitemaker and Neeltje A. Kootstra 327
26 Quantitative Evaluation of HIV and SIV Co-Receptor Use
With GHOST(3) Cell Assay
Dalma Vodros and Eva Maria Fenyo 333
27 Phenotypic Characterization of Blood Monocytes
From HIV-lnfected Individuals
Philip ). Ellery and Suzanne M. Crowe 343
28 Methods to Determine HIV-1 Ex Vivo Fitness
Awet Abraha, Ryan M. Troyer, Miguel E. Quinones-Mateu,
and Eric J. Arts 355
29 A Yeast Recombination-Based Cloning System
to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes
Dawn M. Moore, Eric ). Arts, Yong Gao, and Andre J. Marozsan ... 369
30 PCR Amplification, Cloning, and Construction of HIV-1 Infectious
Molecular Clones From Virtually Full-Length HIV-1 Genomes
Philip K. Ehrenberg and Nelson L. Michael 387
31 Amplification and Cloning of Near Full-Length HIV-2 Genomes
Feng Gao 399
32 Growth and Manipulation of a Human T-Cell Leukemia Virus
Type 2 Full-Length Molecular Clone
Matthew Anderson and Patrick L. Green 409
33 Construction and Analysis of Genomic, Full-Length
Infectious Foamy Virus DNA Clones
Roman Wirtz and Martin Lochelt 423
34 Molecular Characterization of Proteolytic Processing
of the Gag Proteins of Human Spumaretrovirus
Klaus-lngmar Pfrepper and Rolf M. Fliigel 435
35 Assessing the Relative Efficacy of Antiretroviral Activity
of Different Drugs on Macrophages
Stefano Aquaro and Carlo-Federico Perno 445
36 Gene Expression Profiling of HIV-1 Infection
Using cDNA Microarrays
Angelique B. van t Wout 455
x Contents
37 Determination of DC-SIGN and DC-SIGNR Repeat Region
Variations
Huanliang Liu and Tuofu Zhu 471
Index 483
|
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| spelling | Human retrovirus protocols virology and molecular biology ed. by Tuofu Zhu Totowa, NJ Humana Press 2005 XIV, 494 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 304 Includes bibliographical references and index Rétrovirus - Manuels de laboratoire Retroviruses Laboratory manuals Retroviren (DE-588)4121587-4 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Retroviren (DE-588)4121587-4 s Untersuchungsmethode (DE-588)4324907-3 s b DE-604 Zhu, Tuofu Sonstige oth Methods in molecular biology 304 (DE-604)BV035362695 304 http://www.loc.gov/catdir/toc/ecip053/2004026213.html Table of contents Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013204032&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013204032&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Human retrovirus protocols virology and molecular biology Methods in molecular biology Rétrovirus - Manuels de laboratoire Retroviruses Laboratory manuals Retroviren (DE-588)4121587-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)4121587-4 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Human retrovirus protocols virology and molecular biology |
| title_auth | Human retrovirus protocols virology and molecular biology |
| title_exact_search | Human retrovirus protocols virology and molecular biology |
| title_full | Human retrovirus protocols virology and molecular biology ed. by Tuofu Zhu |
| title_fullStr | Human retrovirus protocols virology and molecular biology ed. by Tuofu Zhu |
| title_full_unstemmed | Human retrovirus protocols virology and molecular biology ed. by Tuofu Zhu |
| title_short | Human retrovirus protocols |
| title_sort | human retrovirus protocols virology and molecular biology |
| title_sub | virology and molecular biology |
| topic | Rétrovirus - Manuels de laboratoire Retroviruses Laboratory manuals Retroviren (DE-588)4121587-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Rétrovirus - Manuels de laboratoire Retroviruses Laboratory manuals Retroviren Untersuchungsmethode Aufsatzsammlung |
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