RNA silencing: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in molecular biology
309 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis Klappentext |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 338 S. graph. Darst. |
| ISBN: | 1588294366 |
Internformat
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| 245 | 1 | 0 | |a RNA silencing |b methods and protocols |c ed. by Gordon Carmichael |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2005 | |
| 300 | |a XI, 338 S. |b graph. Darst. | ||
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| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a RNA |2 cabt | |
| 650 | 7 | |a Interference |2 cabt | |
| 650 | 7 | |a Gene Expression |2 cabt | |
| 650 | 7 | |a Rna |2 larpcal | |
| 650 | 4 | |a aSmall interfering RNA |a vLaboratory manuals | |
| 650 | 4 | |a aGene silencing |a vLaboratory manuals | |
| 650 | 4 | |a aRNA Interference | |
| 650 | 4 | |a aGene Expression Regulation |a xgenetics | |
| 650 | 4 | |a aMicroRNAs |a xphysiology | |
| 650 | 4 | |a aRNA, Small Interfering |a xphysiology | |
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Datensatz im Suchindex
| _version_ | 1804133393600872448 |
|---|---|
| adam_text | RNA SILENCING / CARMICHAEL, GORDON : C2005
TABLE OF CONTENTS / INHALTSVERZEICHNIS
IDENTIFICATION OF COMPONENTS OF RNAI PATHWAYS USING THE TANDEM AFFINITY PURIFICATION METHOD / MIKIKO C. SIOMI AND HARUHIKO SIOMI
SEPARATION OF DROSOPHILA RNA SILENCING COMPLEXES BY NATIVE GEL ELECTROPHORESIS / JOHN W. PHAM AND ERIK J. SONTHEIMER
PREPARATION AND ANALYSIS OF DROSHA / YOONTAE LEE AND V. NARRY KIM
RNA INTERFERENCE IN CAENORHABDITIS ELEGANS / NATHANIEL R. DUDLEY AND BOB GOLDSTEIN
DOWN-REGULATING GENE EXPRESSION BY RNA INTERFERENCE IN TRYPANOSOMA BRUCEI / CHRISTINE E. CLAYTON ... [ET AL.]
ANALYSIS OF DOUBLE-STRANDED RNA AND SMALL RNAS INVOLVED IN RNA-MEDIATED TRANSCRIPTIONAL GENE SILENCING / M. FLORIAN METTE ... [ET AL.]
STRATEGIES FOR THE DESIGN OF RANDOM SIRNA LIBRARIES AND THE SELECTION OF ANTI-GFP SIRNAS / MOULDY SIOUD AND SEBASTIONA WULCHLI
SILENCING GENE EXPRESSION WITH DICER-GENERATED SIRNA POOLS / JASON W. MYERS AND JAMES E. FERRELL, JR.
INHIBITION OF GENE EXPRESSION IN VIVO USING MULTIPLEX SIRNA / SUNG-SUK CHAE AND TIMOTHY HLA
GENE SILENCING BY A DNA VECTOR-BASED RNAI TECHNOLOGY / GUANGCHAO SUI AND YANG SHI
PLASMID-BASED RNA INTERFERENCE: CONSTRUCTION OF SMALL-HAIRPIN RNA EXPRESSION VECTORS / SCOTT Q. HARPER AND BEVERLY L. DAVIDSON
SIRNA DELIVERY IN VIVO / MOULDY SIOUD
PEPTIDE-BASED STRATEGY FOR SIRNA DELIVERY INTO MAMMALIAN CELLS / FEDERICA SIMEONI ... [ET AL.]
LENTIVIRAL VECTOR DELIVERY OF SIRNA AND SHRNA ENCODING GENES INTO CULTURED AND PRIMARY HEMATOPOIETIC CELLS / MINGJIE LI AND JO ROSSI
EXON-SPECIFIC RNA INTERFERENCE: A TOOL TO DETERMINE THE FUNCTIONAL RELEVANCE OF PROTEINS ENCODED BY ALTERNATIVELY SPLICED MRNAS / ALICIA M. CELOTTO, JOO-WON LEE, AND BRENTON R. GRAVELEY
IMMUNOPRECIPITATION OF MICRORNPS AND DIRECTIONAL CLONING OF MICRORNAS / ELSA MANIATAKI, MARIA DELS ANGELS DE PLANELL SAGUER, AND ZISSIMOS MOURELATOS
DETECTION OF MICRORNAS AND ASSAYS TO MONITOR MICRORNA ACTIVITIES IN VIVO AND IN VITRO / MARIANTHI KIRIAKIDOU ... [ET AL.]
HIGH THROUGHPUT ANALYSIS OF MICRORNA GENE EXPRESSION USING SENSITIVE CDNA PROBES / MOULDY SIOUD AND OEYSTEIN ROESOK
MODIFICATION OF HUMAN U1 SNRNA FOR INHIBITION OF GENE EXPRESSION AT THE LEVEL OF PRE-MRNA / PENG LIU ... [ET AL.].
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
The past decade has witnessed a true revolution in our understanding of how
reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and
anticancer
guished researchers in the field describe in step-by-step detail their best methods for the design, preparation,
and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include
methods addressing the biochemical aspects of the silencing machinery,
organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing,
preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific
isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful
Methods in Molecular Biology™series format, each offering step-by-step laboratory instructions, an intro¬
duction outlining the principle behind the technique, lists of the necessary equipment and reagents, and
tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art,
investigators a host of optimized and validated techniques to study
successful practical applications.
Readily reproducible methods for
cells and organisms applications
Techniques to study the silencing mechanism and to
prepare the key reagents involved avoiding known pitfalls
Step-by-step instructions to ensure successful results
|
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| id | DE-604.BV019869483 |
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| indexdate | 2024-07-09T20:08:00Z |
| institution | BVB |
| isbn | 1588294366 |
| language | English |
| lccn | 2004023107 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-013193815 |
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| physical | XI, 338 S. graph. Darst. |
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| spelling | RNA silencing methods and protocols ed. by Gordon Carmichael Totowa, NJ Humana Press 2005 XI, 338 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 309 Includes bibliographical references and index RNA cabt Interference cabt Gene Expression cabt Rna larpcal aSmall interfering RNA vLaboratory manuals aGene silencing vLaboratory manuals aRNA Interference aGene Expression Regulation xgenetics aMicroRNAs xphysiology aRNA, Small Interfering xphysiology Geninaktivierung (DE-588)4820638-6 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Geninaktivierung (DE-588)4820638-6 s DE-604 Genexpression (DE-588)4020136-3 s Genregulation (DE-588)4122166-7 s b DE-604 Carmichael, Gordon G. Sonstige oth Methods in molecular biology 309 (DE-604)BV035362695 309 http://www.loc.gov/catdir/toc/ecip051/2004023107.html Table of contents LoC Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013193815&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013193815&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | RNA silencing methods and protocols Methods in molecular biology RNA cabt Interference cabt Gene Expression cabt Rna larpcal aSmall interfering RNA vLaboratory manuals aGene silencing vLaboratory manuals aRNA Interference aGene Expression Regulation xgenetics aMicroRNAs xphysiology aRNA, Small Interfering xphysiology Geninaktivierung (DE-588)4820638-6 gnd Genregulation (DE-588)4122166-7 gnd RNS (DE-588)4076759-0 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4820638-6 (DE-588)4122166-7 (DE-588)4076759-0 (DE-588)4020136-3 (DE-588)4143413-4 |
| title | RNA silencing methods and protocols |
| title_auth | RNA silencing methods and protocols |
| title_exact_search | RNA silencing methods and protocols |
| title_full | RNA silencing methods and protocols ed. by Gordon Carmichael |
| title_fullStr | RNA silencing methods and protocols ed. by Gordon Carmichael |
| title_full_unstemmed | RNA silencing methods and protocols ed. by Gordon Carmichael |
| title_short | RNA silencing |
| title_sort | rna silencing methods and protocols |
| title_sub | methods and protocols |
| topic | RNA cabt Interference cabt Gene Expression cabt Rna larpcal aSmall interfering RNA vLaboratory manuals aGene silencing vLaboratory manuals aRNA Interference aGene Expression Regulation xgenetics aMicroRNAs xphysiology aRNA, Small Interfering xphysiology Geninaktivierung (DE-588)4820638-6 gnd Genregulation (DE-588)4122166-7 gnd RNS (DE-588)4076759-0 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | RNA Interference Gene Expression Rna aSmall interfering RNA vLaboratory manuals aGene silencing vLaboratory manuals aRNA Interference aGene Expression Regulation xgenetics aMicroRNAs xphysiology aRNA, Small Interfering xphysiology Geninaktivierung Genregulation RNS Genexpression Aufsatzsammlung |
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