Cytometry: new developments
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
2004
|
| Ausgabe: | 4. ed. |
| Schriftenreihe: | Methods in cell biology
75 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Bis 3. ed. 2001 und ab 5. ed. 2011 mehrbd. erschienen |
| Beschreibung: | XXXVII, 876 S., [17] Bl. Ill., graph. Darst. |
| ISBN: | 0124802834 0125641702 |
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| 650 | 4 | |a Cytologie - Technique | |
| 650 | 7 | |a Cytometrie |2 gtt | |
| 650 | 4 | |a Cytométrie de flux | |
| 650 | 4 | |a Cytophotométrie | |
| 650 | 4 | |a Cell Separation |x methods | |
| 650 | 4 | |a Cytogenetics | |
| 650 | 4 | |a Cytogenetics |x methods | |
| 650 | 4 | |a Cytophotometry | |
| 650 | 4 | |a Flow Cytometry |x methods | |
| 650 | 4 | |a Flow cytometry | |
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Datensatz im Suchindex
| _version_ | 1804133196803080192 |
|---|---|
| adam_text | CONTENTS
Contributors xvii
Preface xxiii
Preface to the Third Edition xxvii
Preface to the Second Edition xxxi
Preface to the First Edition xxxv
PART I Instrumentation/Fluorochromes
1. Optimization of Emission Optics for Multicolor Flow Cytometry
Nicole Baumgarth and Marty Bigos
I. Introduction 3
II. Fluorochromes 4
III. Hardware Components Affecting Light Collection 8
IV. Maximizing Signal Intensities 11
V. Maximizing Signal Collection Quality 11
VI. Emission Collection Optimization 14
VII. Testing Emission Optics 16
References 21
2. Two Photon Tissue Cytometry
Timothy Ragan, Ki Heart Kim, Karsten Bahlmann, and Peter T. C. So
I. Introduction 23
II. Technical Development of Two Photon Tissue Cytometry 24
III. Experimental Demonstration of Two Photon Tissue Cytometry 32
IV. Future Outlook 37
V. Conclusion 37
References 38
Contents
3. Confocal Microscopy: Quantitative Analytical Capabilities
Jurek W. Dobrucki
I. Introduction 42
II. Cartesian Coordinates, Distance, Size, Shape, and Volume in
Confocal Microscopy 43
III. Measurements of Fluorescence Intensity in Confocal Microscopy 53
IV. Time Resolution in Confocal Microscopy 57
V. Confocal Techniques Based on Measurements of Fluorescence
Intensity vs Time 57
VI. Spectrally Resolved Confocal Microscopy 64
VII. Conclusion 69
References 70
4. Surface Plasmon—Coupled Emission: New Technology for Studying
Molecular Processes
Zygmunt Gryczynski, Ignacy Gryczynski, Evgenia Matveeva, Joanna Malicka,
Kazimierz Nowaczyk, and Joseph R. Lakouncz
I. Introduction 73
II. Overview of Surface Plasmon Resonance 75
III. Surface Plasmon Coupled Emission 80
IV. Experimental Studies of Surface Plasmon—Coupled Emission 83
V. Practical Applications 90
VI. Conclusion 102
References 102
5. Cytometry of Fluorescence Resonance Energy Transfer
Gyorgy Vereb,Janos Matko, andjanos SzollSsi
I. Introduction 105
II. Theory of FRET 107
III. Measuring FRET 109
IV. Applications 132
V. Perspectives 142
VI. Conclusions 145
References 146
6 The Rainbow of Fluorescent Proteins
David W. Galbraith
I. The Fluorescent Proteins 153
II. Flow Analysis Using Fluorescent Proteins 159
Contents vii
III. Flow Sorting Using Fluorescent Proteins 163
IV. Conclusion 164
References 165
7. Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates
Xingyong Wu and Marcel P. Bruchez
I. Introduction 171
II. Selection of QDs and Their Conjugates 172
III. Labeling of Fixed Cells for Fluorescence Microscopy 173
IV. Measurements on Living Cells Using QD Conjugates 179
V. Conclusion 181
References 182
8. Next Generation Laser Scanning Cytometry
Ed Luther, Louis Kamentsky, Melvin Henriksen, and Elena Holden
I. Introduction 186
II. LSC Hybrid Technology 186
III. Description of the LSC Platform 188
IV. Selected Applications 203
V. Utility of Solid Phase Imaging Cytometry in Life Science Research
and Drug Discovery 217
References 217
PART II General Techniques
9. Biohazard Sorting
Ingrid Schmid, Mario Roederer, Richard A. Koup, David Ambrozak,
and Stephen P. Perfetto
I. Introduction 221
II. Critical Aspects of the Procedure 222
III. Applications and Future Directions 238
References 239
10. Guidelines for the Presentation of Flow Cytometric Data
Mario Roederer, Zbigniew Darzynkiewicz, and David R. Parks
I. Introduction 241
Contents
II. General Principles of Graphical Presentation 243
III. Statistics 251
IV. Subset Analysis 253
V. Conclusion 256
References 256
11. Mechanism of Antitumor Drug Action Assessed by Cytometry
Frank Traganos
I. Introduction 258
II. Choice of a Model System 258
III. Drug Dose and Length of Exposure 263
IV. Single Parameter DNA Histogram Analysis 265
V. Multiparameter Approaches 274
VI. Data Presentation 298
VII. Summary and Future Directions 299
References 301
12. Cytometric Methods to Detect Apoptosis
Zhigniew Darzynkiewicz, Xuan Huang, Masaki Okafuji, and Malcolm A. King
I. Introduction 308
II. Physical and Molecular Features of Cells Dying by Apoptosis or Necrosis 310
III. Different Time Windows for Detection of Apoptosis: Possible Source
of Error in Measurement of Incidence of Apoptosis 316
IV. Light Scattering Properties of Cells Dying by Apoptosis 317
V. Mitochondrial Transmembrane Potential (A*?,,,) 318
VI. Activation of Caspases 321
VII. Annexin V Binding 325
VIII. DNA Fragmentation 326
IX. Susceptibility of DNA to Denaturation 329
X. Activation of Tissue Transglutaminase 331
XI. Measuring Incidence of Apoptosis: Which Method to Choose? 332
References 335
13. Real Time Analysis of Apoptosis In Vivo
Pui Lee and Mark S. Segal
I. Introduction 343
II. In Vivo Membrane Markers 344
III. A New Generation of Caspase Markers 348
IV. Conclusion 351
References 351
Contents ix
14. Detection of DNA Damage in Individual Cells by Analysis of Histone
H2AX Phosphorylation
Peggy L. Olive
I. Introduction 355
II. Considerations in the Use of yH2AX as a Measure of
Double Stranded Breaks 356
III. Methods of Analysis 359
IV. Typical Results 363
V. Possible Applications 370
VI. Conclusion 371
References 371
15. Cytometry of Freshwater Phytoplankton
Jorg Toepel, Christian Wilhelm, Artnin Meister, Annette Becker,
and Maria del Carmen Martinez Ballesta
I. Introduction 376
II. Background 378
III. Methods 387
IV. Results 393
V. Limitations and Applications 399
VI. Future Directions 403
References 403
16. Multiplexed Microsphere Assays for Protein and DNA Binding Reactions
Kathryn L. Kellar and Kerry G. Oliver
I. Introduction 410
II. Technology and Instrumentation 411
III. Methods 415
IV. Results 418
V. Software 420
VI. Critical Aspects of the Methodology 421
VII. Comparison with Other Methods 424
VIII. Future Directions 425
References 426
Contents
PART III Immunology/T Cell Responses
17. Flow Cytometry Applications of MHC Tetramers
John D. Altman
I. Introduction 433
II. Materials and Methods 436
III. Analyzing Tetramer Data 444
IV. Combining Tetramer Staining with Functional Assays 446
V. Conclusion 447
References 448
18. Use of Peptides and Peptide Libraries as T Cell Stimulants in Flow Cytometric Studies
Georgy Cherepnev, Hans Dieter Volk, and Florian Kern
I. Introduction 454
II. Background 455
III. Results/Discussion of the Literature 459
IV. Technical Considerations 464
V. Specific Applications 467
VI. Potential Clinical Use 471
VII. Protocols 473
References 475
19. Flow Cytometric Analysis of Human Antigen Specific T Cell Proliferation
Jason M. Brenchley and Daniel C. Douek
I. Introduction and Background 482
II. Methods 483
III. Results 484
IV. Critical Aspects of the Methodology 487
V. Pitfalls and Misinterpretation of the Data 490
VI. Future Directions 491
References 492
20. Detection of T Cell Degranulation: CD 107a and b
Michael R. Belts and Richard A. Koup
I. Introduction: Methods to Assess CD8+ T Cell Function 497
II. Materials and Methods 500
HI. Results 504
Contents XI
IV. Critical Aspects 505
V. Applications 508
References 510
21. T Cell Responses to Cancer
Peter P. Lee
I. Introduction 514
II. Methods to Identify and Enumerate TAA Specific T Cells 516
III. Important Considerations 520
IV. Methods to Further Analyze In Vivo Biology of Antitumor T Cells 522
V. Novel Issues and Methods 524
VI. Conclusion 527
References 528
PART IV Multi Color Immunophenotyping
22. Multicolor Flow Cytometric Analysis in SIV Infected Rhesus Macaque
Joshua M. Walker, Holden T. Maecker, Vernon C. Maino, and Louis J. Picker
I. Introduction 536
II. Methods 537
III. Flow Cytometry Instrumentation and Software 538
IV. Instrument Setup 539
V. Critical Aspects of the Technology 542
VI. Application of 10 Color Analysis to SIV Infection 548
VII. Conclusion 554
References 555
23. Multicolor Immunophenotyping: Human Immune System Hematopoiesis
Brent Wood
I. Introduction 559
II. Methodology 560
III. Normal Immunophenotypic Patterns of Maturation 561
IV. Abnormal Immunophenotypic Patterns of Maturation 573
References 575
Contents
24. Multicolor Immunophenotyping: Human Mature Immune System
Stephen C. De Rosa
I. Introduction 577
II. Choice of Markers 578
III. Staining Panels: Matching Fluorochrome with Cell Marker 586
IV. Conclusion 592
References 593
25. Differential Diagnosis of T Cell Lymphoproliferative Disorders by Flow Cytometry
Multicolor Immunophenotyping. Correlation with Morphology
Wojciech Gorczyca
I. Introduction 596
II. Materials 596
III. Methods 597
IV. Identification of Abnormal T Cell Population by Flow Cytometry 598
V. Precursor T Lymphoblastic Lymphoma/Leukemia 601
VI. Peripheral (Mature/Post thymic) Lymphoma Versus Precursor
T Lymphoblastic Lymphoma/Leukemia 601
VII. Thymocytes from Thymic Hyperplasia/Thymoma Versus Precursor
T Lymphoblastic Lymphoma/Leukemia 604
VIII. Mature T Cell Lymphoproliferative Disorders 604
IX. CD117 Expression in T Cell Lymphoproliferations 616
X. Blastic NK Cell Lymphoma (DC 2 Acute Leukemia) 618
XI. Conclusion 618
References 619
26. Isolation and Immunophenotyping of Human and Rhesus Macaque
Dendritic Cells
Karin Lore
I. Introduction 623
II. In Vitro Differentiation of Monocyte Derived Dendritic Cells 625
III. Detection and Sorting Procedures of Subsets of Dendritic Cells 625
IV. Culture and Activation of Dendritic Cells 635
References 637
27. B Cell Immunophenotyping
Nicole Baumgarth
I. Introduction 643
Contents xiii
II. Immunophenotyping of B Cell Developmental Stages in
the Bone Marrow 644
III. Peripheral B Cell Populations 649
IV. Antigen Induced B Cell Subsets 655
V. Antigen Specific B Cells 658
References 660
PART V Other Topics in Immunology
28. Flow Cytometry Immunophenotypic Characteristics of Monocytic Population
in Acute Monocytic Leukemia (AML M5), Acute Myelomonocytic Leukemia
(AML M4), and Chronic Myelomonocytic Leukemia (CMML)
Wojciech Gorczyca
I. Introduction 666
II. Materials 666
III. Methods 667
IV. Acute Monocytic Leukemia 668
V. Chronic Myelomonocytic Leukemia 668
VI. Acute Myelomonocytic Leukemia 668
VII. Differential Diagnosis 670
VIII. Conclusion 676
References 676
29. Phagocyte Function
Gregor Rothe and Mariam Kbuche
I. Introduction 680
II. Background 690
III. Methods 694
IV. Results 697
V. Pitfalls and Misinterpretation of the Data 698
VI. Comparison with Other Methods 700
VII. Applications and Biomedical Information 701
VIII. Future Directions 703
References 703
30. Neutralizing Antibody Quantification by Flow Cytometry
John R. Mascola
I. Introduction 709
av Contents
II. Background 710
III. Results 712
IV. Discussion and Key Points 714
References 715
PART VT Cytogenetics
31. Telomere Length Measurements Using Fluorescence In Situ Hybridization
and Flow Cytometry
Gabriela M. Baerlocher and Peter M. Lansdorp
I. Introduction 720
II. Background 725
III. Methods 727
IV. Results 739
V. Critical Aspects of Methodology 742
VI. Pitfalls and Misinterpretation of Data 744
VII. Comparison with Other Methods 745
VIII. Applications 746
IX. Future Directions 748
References 749
32. Detecting Copy Number Changes in Genomic DNA: MAPH and MLPA
Stefan J. White, Martijn H. Breuning, andjohan T. den Dunnen
I. Introduction 751
II. Reactions 752
III. Analysis 756
IV. Applications 762
V. Future Possibilities 764
References 766
33. Genomic Array Technology
Heike Fiegler and Nigel P. Carter
I. Introduction 769
II. Construction of Genomic Clone Arrays Using DOP PCR Amplification 770
III. Validation of Array Performance and Data Analysis 771
IV. Array CGH 776
V. Array CGH for Cytogenetic Analyses 778
VI. Array Painting 779
Contents XV
VII. Application of Array CGH and Array Painting for Complete
Cytogenetic Analyses 780
VIII. ChIP on Genomic Clone Arrays 782
IX. Conclusion ¦ 782
References 784
34. Prospects for In Situ Analyses of Individual and Complexes of DNA, RNA, and Protein
Molecules with Padlock and Proximity Probes
Ulf Landegren, Mats Nilsson, Mats Gullberg, Ola Soderberg, Malin Jarvius,
Chatarina Larsson, and Jonas Jarvius
I. Need for Single Molecule In Situ Analyses 787
II. Problem of Specificity and Sensitivity 788
III. Single or Dual Recognition Assays 789
IV. Some Possible Assay Formats 790
V. Specific Detection of Macromolecules followed by Selective
In Situ Amplification 794
VI. Future Directions 795
References 795
35. The Use of Subtelomeric Probes to Study Mental Retardation
SamanthaJ. L. Knight and Jonathan Flint
I. Introduction 800
II. Background 802
III. Methods 806
IV. Results 815
V. Critical Aspects of the Methodologies 815
VI. Limitations, Pitfalls, and Misinterpretation of Data 820
VII. Comparison with Other Methods 824
VIII. Applications 825
IX. Future Directions 826
References 828
Index 833
Volumes in Series 869
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| spelling | Cytometry new developments ed. by Zbigniew Darzynkiewicz ... 4. ed. Amsterdam [u.a.] Elsevier 2004 XXXVII, 876 S., [17] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 75 Bis 3. ed. 2001 und ab 5. ed. 2011 mehrbd. erschienen Cytogénétique Cytologie - Technique Cytometrie gtt Cytométrie de flux Cytophotométrie Cell Separation methods Cytogenetics Cytogenetics methods Cytophotometry Flow Cytometry methods Flow cytometry Immunophenotyping methods Cytometrie (DE-588)4148710-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Cytometrie (DE-588)4148710-2 s b DE-604 Darzynkiewicz, Zbigniew Sonstige oth Methods in cell biology 75 (DE-604)BV002534878 75 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013060593&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytometry new developments Methods in cell biology Cytogénétique Cytologie - Technique Cytometrie gtt Cytométrie de flux Cytophotométrie Cell Separation methods Cytogenetics Cytogenetics methods Cytophotometry Flow Cytometry methods Flow cytometry Immunophenotyping methods Cytometrie (DE-588)4148710-2 gnd |
| subject_GND | (DE-588)4148710-2 (DE-588)4143413-4 |
| title | Cytometry new developments |
| title_auth | Cytometry new developments |
| title_exact_search | Cytometry new developments |
| title_full | Cytometry new developments ed. by Zbigniew Darzynkiewicz ... |
| title_fullStr | Cytometry new developments ed. by Zbigniew Darzynkiewicz ... |
| title_full_unstemmed | Cytometry new developments ed. by Zbigniew Darzynkiewicz ... |
| title_short | Cytometry |
| title_sort | cytometry new developments |
| title_sub | new developments |
| topic | Cytogénétique Cytologie - Technique Cytometrie gtt Cytométrie de flux Cytophotométrie Cell Separation methods Cytogenetics Cytogenetics methods Cytophotometry Flow Cytometry methods Flow cytometry Immunophenotyping methods Cytometrie (DE-588)4148710-2 gnd |
| topic_facet | Cytogénétique Cytologie - Technique Cytometrie Cytométrie de flux Cytophotométrie Cell Separation methods Cytogenetics Cytogenetics methods Cytophotometry Flow Cytometry methods Flow cytometry Immunophenotyping methods Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013060593&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT darzynkiewiczzbigniew cytometrynewdevelopments |