Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Elektronisch Software E-Book |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Ulm, Univ., Diss., 2004 |
| Beschreibung: | 1 CD-R 12 cm |
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Datensatz im Suchindex
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| adam_text | CONTENT
ABBREVIATIONS 3
1. INTRODUCTION 5
2. MATERIALS AND METHODS 13
2.1. Materials 13
2.1.1. Cell lines 13
2.1.2. Reagents 13
2.7.4. Equipments 14
2.2. Methods 14
2.2.1. Set up of home made cell assay incubator for Ca2+ measure 14
2.2.2. Cell culture 15
2.2.3. Intracellular Ca2+ staining 15
2.2.4. Confocal imaging of Ca2+ and apoptosis using Fluo 4 and PI 16
2.2.5. Preparation ofCa2+ signal blocking and chelating agents 16
2.3. Assay condition 17
3. RESULTS 19
3.1. Oxygen dependent pressure induced Ca2+ release 19
3.2. Effect of blockers and chelators in Ca2+ signal 19
3.3. Involvement of P2X7 receptors 21
3.4. Quantification of intracellular Ca2+ 22
3.5. Ca2+ signal under normal atmospheric pressure 25
3.6. Morphological changes and Apoptosis 25
3.7. Oxygen determines Ca2+ signal 27
3.8. Intercellular communication of Ca2+ signal 27
4. FIGURES AND LEGENDS 28
5. DISCUSSION 65
5.1. Ca2+signal 65
5.2. Effect of paxilline on intracellular Ca2+ in Hela cells 68
5.3. Effect of ATP receptors in Ca2+ signal 69
5.4. Apoptosis morphological changes 70
5.5. Nonspecific Ca2+signals 74
5.6. Choice of intracellular Ca2+ indicators 75
5.7. Gas dependent Ca2+ signal effects 76
6. SUMMARY 78
7. REFFERENCE 80
8. ACKNOWLEDGEMENT 92
9. CURRICULUM VITAE 93
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| language | English |
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| spelling | Ma, Xueling 1964- Verfasser (DE-588)129493252 aut Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells by Ma, Xueling 2004 1 CD-R 12 cm txt rdacontent c rdamedia cd rdacarrier Ulm, Univ., Diss., 2004 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013009277&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ma, Xueling 1964- Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells |
| subject_GND | (DE-588)4113937-9 |
| title | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells |
| title_auth | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells |
| title_exact_search | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells |
| title_full | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells by Ma, Xueling |
| title_fullStr | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells by Ma, Xueling |
| title_full_unstemmed | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells by Ma, Xueling |
| title_short | Gas-pressure induced intracellular calcium signalling and apoptosis in HeLa cells |
| title_sort | gas pressure induced intracellular calcium signalling and apoptosis in hela cells |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013009277&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT maxueling gaspressureinducedintracellularcalciumsignallingandapoptosisinhelacells |