Molecular toxicology protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in molecular biology
291 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 489 S. Ill., graph. Darst. |
| ISBN: | 1588290840 |
Internformat
MARC
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| 245 | 1 | 0 | |a Molecular toxicology protocols |c ed. by Phouthone Keohavong ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2005 | |
| 300 | |a XIV, 489 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 291 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Toxicologie moléculaire - Manuels de laboratoire | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a DNA Adducts |x analysis | |
| 650 | 4 | |a DNA Mutational Analysis | |
| 650 | 4 | |a DNA Repair | |
| 650 | 4 | |a Molecular toxicology |v Laboratory manuals | |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gentoxikologie |0 (DE-588)4338045-1 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Labortechnik |0 (DE-588)4123602-6 |D s |
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| 700 | 1 | |a Keohavong, Phouthone |e Sonstige |4 oth | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 1-59259-840-4 |
| 830 | 0 | |a Methods in molecular biology |v 291 |w (DE-604)BV035362695 |9 291 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-012943852 | ||
Datensatz im Suchindex
| _version_ | 1804132975300837376 |
|---|---|
| adam_text | Contents
Preface v
Contributors i
Part I: Analysis of DNA Adducts
1 32P Postlabeling Analysis of DNA Adducts
David H. Phillips, Alan Hewer, and Volker M. Arlt 3
2 Modification of the 32P Postlabeling Method to Detect a Single
Adduct Species as a Single Spot
Masako Ochiai, Takashi Sugimura, and Minako Nagao 13
3 DNA Isolation and Sample Preparation for Quantification
of Adduct Levels by Accelerator Mass Spectrometry
Karen H. Dingley, Esther A. Ubick, John S. Vogel,
and Kurt W. Haack 21
4 Fluoroimaging Based Immunoassay of DNA Photoproducts
in Ultraviolet B Irradiated Tadpoles
lovanna Pandelova, Stephen R. Hewitt, and John B. Hays 29
5 Analysis of DNA Strand Cleavage at Abasic Sites
Walter A. Deutsch and Vijay Hegde 39
Part II: Detection of Chromosomal and Genome Wide Damage
6 Premature Chromosome Condensation in Human Resting Peripheral
Blood Lymphocytes for Chromosome Aberration Analysis Using
Specific Whole Chromosome DNA Hybridization Probes
Pataje G. S. Prasanna and William F. Blakely 49
7 Mutagen lnduced Chromatid Breakage as a Marker of Cancer Risk
Xifeng Wu, Yun Ling Zheng, and T. C. Hsu 59
8 Flow Cytometric Analysis of Micronuclei in Erythrocytes
Jan Grawe 69
9 The Comet Assay: A Sensitive Genotoxicity Test
for the Detection of DNA Damage
Giinter Speit and Andreas Hartmann 85
10 Computerized Image Analysis Software for the Comet Assay
R. C. Chaubey 97
vii
viii Contents
11 The Comet FISH Technique: A Tool for Detection of Specific
DNA Damage and Repair
Alexander Rapp, Michael Hausmann, and Karl Otto Greulich 107
12 DNA Double Strand Break Damage and Repair
Assessed by Pulsed Field Gel Electrophoresis
Nina Joshi and Stephen G. Grant 121
Part III: Detection and Characterization of Surrogate Gene Mutation
13 Analysis of In Vivo Mutation in the Hprt and 77c Genes
of Mouse Lymphocytes
Vasily N. Dobrovolsky, Joseph G. Shaddock,
and Robert H. Heflich 133
14 Quantifying In Vivo Somatic Mutations
Using Transgenic Mouse Model Systems
Roy R. Swiger 145
15 Methods for Detecting Somatic Mutations In Vitro:
The Human T Cell Cloning Assay Selecting for HPRT Mutants
Sai MeiHou 155
16 Molecular Analysis of Mutations in the Human HPRT Gene
Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant 161
17 Simultaneous Quantification of t(14;18) and
HPRT Exon 2/3 Deletions in Human Lymphocytes
fames C. Fuscoe 171
18 The GPA In Vivo Somatic Mutation Assay
Stephen G. Grant 179
19 Flow Cytometric Measurement of Mutant T Cells With Altered
Expression of TCR: Detecting Somatic Mutations in Humans
and Mice
Seishi Kyoizumi, Yoichiro Kusunoki, and Tomonori Hayashi 197
Part IV: Detection and Characterization of Cancer Gene Mutation
20 Mutation Screening of the TP53 Gene
by Temporal Temperature Gradient Gel Electrophoresis
Therese Serlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind,
Ragnhild Lothe, and Anne Lise Berresen Dale 207
21 Analysis of K RAS and P53 Mutations in Sputum Samples
Weimin Gao and Phouthone Keohavong 217
22 Allele Specific Competitive Blocker PCR Detection
of Rare Base Substitution
Barbara L Parsons, Page B. McKinzie, and Robert H. Heflich 235
Contents ix
23 Gel Based Nonradioactive Single Strand Conformational
Polymorphism and Mutation Detection: Limitations and Solutions
Vibhuti Gupta, Reetakshi Arora, Anand Ranjan,
Narendra K. Bairwa, Dheeraj K. Malhotra,
P. T. Udhayasuriyan, Anjana Saha, and Ramesh Bamezai 247
24 Detection and Characterization of Oncogene Mutations
in Preneoplastic and Early Neoplastic Lesions
Toshinari Minamoto 263
25 Detection of DNA Double Strand Breaks and Chromosome
Translocations Using Ligation Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti,
and Andrew T. M. Vaughan 279
Part V: Analysis of DNA Repair Mechanisms
26 Microsatellite Instability: An Indirect Assay to Detect Defects
in the Cellular Mismatch Repair Machinery
Anjana Saha, Narendra K. Bairwa, and Ramesh Bamezai 293
27 Unscheduled DNA Synthesis: A Functional Assay
for Global Genomic Nucleotide Excision Repair
Crystal M. Kelly and Jean J. Latimer 303
28 Analysis of DNA Repair Using Transfection Based
Host Cell Reactivation
Jennifer M. Johnson and Jean J. Latimer 321
29 An Immunoassay for Measuring Repair of Ultraviolet Photoproducts
Shirley McCready 337
30 Analysis of DNA Double Strand Break Repair by Nonhomologous
End Joining in Cell Free Extracts From Mammalian Cells
Petra Pfeiffer, Elke Feldmann, Andrea Odersky,
Steffi Kuhfittig Kulle, and Wolfgang Goedecke 351
31 Measuring Recombination Proficiency
in Mouse Embryonic Stem Cells
Andrew J. Pierce and Maria Jasin 373
Part VI: Array Technologies
32 Strategies for Measurement of Biotransformation
Enzyme Gene Expression
Marjorie Romkes and Shama C. Buch 387
33 Genotyping Technologies: Application to Biotransformation
Enzyme Genetic Polymorphism Screening
Marjorie Romkes and Shama C. Buch 399
x Contents
34 TaqMan® Fluorogenic Detection System
to Analyze Gene Transcription in Autopsy Material
Kaori Shintani lshida, Bao Li Zhu, and Hitoshi Maeda 475
35 Development of Quantitative Reverse Transcriptase PCR Assays
for Measuring Gene Expression
Tony E. Godfrey and Lori A. Kelly 423
Part VII: Apoptosis
36 Quantification of Selective Phosphatidylserine Oxidation
During Apoptosis
James P. Fabisiak, Yulia Y. Tyurina, Vladimir A. Tyurin,
and Valerian E. Kagan 449
37 Quantitative Method of Measuring Phosphatidylserine
Externalization During Apoptosis Using Electron Paramagnetic
Resonance Spectroscopy and Annexin Conjugated Iron
James P. Fabisiak, Grigory G. Borisenko, and Valerian E. Kagan 457
38 Detection of Programmed Cell Death in Cells Exposed
to Genotoxic Agents Using a Caspase Activation Assay
Michael E. Gehring and Patrick P. Koty 465
Index 473
|
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| id | DE-604.BV019614301 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T20:01:21Z |
| institution | BVB |
| isbn | 1588290840 |
| language | English |
| lccn | 2004005911 |
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| physical | XIV, 489 S. Ill., graph. Darst. |
| publishDate | 2005 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Molecular toxicology protocols ed. by Phouthone Keohavong ... Totowa, NJ Humana Press 2005 XIV, 489 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 291 Includes bibliographical references and index Toxicologie moléculaire - Manuels de laboratoire Apoptosis DNA Adducts analysis DNA Mutational Analysis DNA Repair Molecular toxicology Laboratory manuals Labortechnik (DE-588)4123602-6 gnd rswk-swf Gentoxikologie (DE-588)4338045-1 gnd rswk-swf Gentoxikologie (DE-588)4338045-1 s Labortechnik (DE-588)4123602-6 s b DE-604 Keohavong, Phouthone Sonstige oth Erscheint auch als Online-Ausgabe 1-59259-840-4 Methods in molecular biology 291 (DE-604)BV035362695 291 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012943852&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular toxicology protocols Methods in molecular biology Toxicologie moléculaire - Manuels de laboratoire Apoptosis DNA Adducts analysis DNA Mutational Analysis DNA Repair Molecular toxicology Laboratory manuals Labortechnik (DE-588)4123602-6 gnd Gentoxikologie (DE-588)4338045-1 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4338045-1 |
| title | Molecular toxicology protocols |
| title_auth | Molecular toxicology protocols |
| title_exact_search | Molecular toxicology protocols |
| title_full | Molecular toxicology protocols ed. by Phouthone Keohavong ... |
| title_fullStr | Molecular toxicology protocols ed. by Phouthone Keohavong ... |
| title_full_unstemmed | Molecular toxicology protocols ed. by Phouthone Keohavong ... |
| title_short | Molecular toxicology protocols |
| title_sort | molecular toxicology protocols |
| topic | Toxicologie moléculaire - Manuels de laboratoire Apoptosis DNA Adducts analysis DNA Mutational Analysis DNA Repair Molecular toxicology Laboratory manuals Labortechnik (DE-588)4123602-6 gnd Gentoxikologie (DE-588)4338045-1 gnd |
| topic_facet | Toxicologie moléculaire - Manuels de laboratoire Apoptosis DNA Adducts analysis DNA Mutational Analysis DNA Repair Molecular toxicology Laboratory manuals Labortechnik Gentoxikologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012943852&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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