Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
Aachen
Shaker
2003
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| Schriftenreihe: | Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel
6 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 115 S. Ill., Darst. |
| ISBN: | 3832216561 |
Internformat
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| 245 | 1 | 0 | |a Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis |c Hany Sahly |
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Datensatz im Suchindex
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CONTENTS PAG
List of abbreviations
Abstract
1. Introduction 1
1.1 Clinical significance of Klebsiella 1
1.2 Pathogenicity factors of Klebsiella 3
1.2.1 Adhesins: Their role in adhesion to and invasion of non phagocytic cells 3
1.2.2 Lipopolysaccharides: Their role in complement activation 6
and lung collectin binding
1.2.3 Capsular polysaccharides 7
1.3 Klebsiella and ankylosing spondylitis (AS) 10
1.3.1 Recent immunological data linking immune response 12
to Klebsiella capsular antigens and AS
2. Objectives of the thesis 13
3. Materials and methods 15
3.1 List of materials and chemicals 15
3.2. Bacterial strains 19
3.3 Human SP D and SP D mutants 20
3.4 Patients and controls 21
3.5 Cell lines and culture conditions 21
3.6 Isolation of the noncapsulated mutants 22
3.7 Genetic manipulation and construction of the capsule 22
switched derivative
3.8 Determination, visualization and analysis of capsular 26
IV
polysaccharides by nuclear magnetic resonance spectroscopy
3.9 Bacteriocin typing 27
3.10 Pulsed field gel electrophoresis (PFGE) 27
3.11 Hemagglutination assay 28
3.12 Internalization and adhesion assays in ileocecal and 29
bladder epithelial cells
3.13 Assay of intracellular survival and replication in 30
ileocecal epithelial cells
3.14 Purification and gel electrophoresis of LPS 30
3.15 Lectin and immunoblotting of LPS 31
3.16 Assay for SP D induced bacterial agglutination 31
3.17 Adhesion assay to alveolar epithelial cells 32
3.18 Preparation of PMNL 32
3.19 Chemiluminescence (CL) response 32
3.20 IgG binding to bacteria 33
3.21 C3b binding to bacteria 34
3.22 Statistical analysis 34
4. Results I: Internalization, adhesion and survival assays in ileocecal 35
epithelial cells
4.1 Genetic, phenotypic, and serological characterization of the 35
parent strains, noncapsulated variants and capsule switched derivative
4.2 Relationship between capsule formation, adhesion, 41
and internalization into epithelial cells
4.3 Bacterial intracellular persistence and replication 48
4.4 Discussion I: interaction between Klebsiella and epithelial cells 49
V
5. Results II: Interaction with SP D 54
5.1 SP D potently agglutinates an 03, but not an Ol, serotype of Klebsiella 54
5.2 Ol and O3 LPS differ in their ability to inhibit SP D 55
dependent agglutination
5.3 Binding of SP D to Klebsiella LPS is influenced by 56
the presence of O antigen
5.4 SP D shows a higher apparent affinity for mature 59
LPS of the O3 serotype
5.5 SP D selectively binds to mature LPS of the O3 and O5 serotypes 61
5.6 SP D does not bind to S LPS from Klebsiella serotypes 63
commonly associated with Klebsiella pneumonia
5.7 SP D preferentially inhibits the adhesion of the O3 serotype 64
of Klebsiella to epithelial cells
5.8 The effects of SP D on bacterial adhesion require 66
the multimerization of trimeric subunits
5.9 Asparagine linked glycosylation of SP D is not 68
required for inhibition of epithelial adhesion
5.10 Discussion II: Interaction of Klebsiella serotypes with SPD 70
5.10.1 Interactions with smooth forms of LPS 70
5.10.2 Serotype dependent inhibition of epithelial adhesion 73
6. Results III: Interaction with PMNL 77
6.1 Relationship between capsular type, capsule formation and PMNL stimulation 77
6.2 IgG and C3b binding to bacteria in relation to the CL response 81
6.3 Discussion III: Interaction with PMNL 82
7. General overview and concluding remarks 85
6 References 88
|
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| discipline | Medizin |
| format | Thesis Book |
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| id | DE-604.BV019590231 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T20:00:58Z |
| institution | BVB |
| isbn | 3832216561 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-012927001 |
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| physical | XII, 115 S. Ill., Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
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| publisher | Shaker |
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| series | Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel |
| series2 | Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel |
| spelling | Sahly, Hany Verfasser (DE-588)142083801 aut Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis Hany Sahly Aachen Shaker 2003 XII, 115 S. Ill., Darst. txt rdacontent n rdamedia nc rdacarrier Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel 6 Zugl.: Tel Aviv, Univ., Diss., 2002 Pathogenese (DE-588)4115512-9 gnd rswk-swf Wirtszelle (DE-588)4232591-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Friedländer-Bakterium (DE-588)4155451-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Friedländer-Bakterium (DE-588)4155451-6 s Wirtszelle (DE-588)4232591-2 s Wechselwirkung (DE-588)4064937-4 s Pathogenese (DE-588)4115512-9 s DE-604 Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel 6 (DE-604)BV010555221 6 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012927001&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sahly, Hany Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis Schriften des Instituts für Medizinische Mikrobiologie und Virologie der Christian-Albrechts-Universität zu Kiel Pathogenese (DE-588)4115512-9 gnd Wirtszelle (DE-588)4232591-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Friedländer-Bakterium (DE-588)4155451-6 gnd |
| subject_GND | (DE-588)4115512-9 (DE-588)4232591-2 (DE-588)4064937-4 (DE-588)4155451-6 (DE-588)4113937-9 |
| title | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis |
| title_auth | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis |
| title_exact_search | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis |
| title_full | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis Hany Sahly |
| title_fullStr | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis Hany Sahly |
| title_full_unstemmed | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis Hany Sahly |
| title_short | Interactions of Klebsiella pneumoniae with host cells and their contribution to pathogenesis |
| title_sort | interactions of klebsiella pneumoniae with host cells and their contribution to pathogenesis |
| topic | Pathogenese (DE-588)4115512-9 gnd Wirtszelle (DE-588)4232591-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Friedländer-Bakterium (DE-588)4155451-6 gnd |
| topic_facet | Pathogenese Wirtszelle Wechselwirkung Friedländer-Bakterium Hochschulschrift |
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| volume_link | (DE-604)BV010555221 |
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