Verfügbarkeit: DNA fingerprinting in plants

DNA fingerprinting in plants: principles, methods, and applications

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Vorheriger Titel:	DNA fingerprinting in plants and fungi
Format:	Buch
Sprache:	English
Veröffentlicht:	Boca Raton [u.a.] Taylor & Francis 2005
Ausgabe:	2. ed.
Schlagworte:	Dna Genética vegetal Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of plants Plants > genetics Genetischer Fingerabdruck Pflanzen Pilze Fingerprint-Verfahren
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	1. Auf. u.d.T.: DNA fingerprinting in plants and fungi
Beschreibung:	444 S. Ill., graph. Darst.
ISBN:	0849314887

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Datensatz im Suchindex

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adam_text	Table of Contents Chapter 1 Repetitive DNA: An Important Source of Variation in Eukaryotic Genomes 1.1 Categories of DNA Sequence Mutations ........................................................2 1.2 Tandem-Repetitive DNA: The Biology of Mini- and Microsatellites ............4 1.2.1 Minisatellites ........................................................................................5 1.2.1.1 Chromosomal Localization and Association with Other Repeats ..................................................................................6 1.2.1.2 Mutability and Evolution ......................................................6 1.2.1.3 Minisatellites in Organellar Genomes ..................................7 1.2.1.4 Potential Functions of Minisatellites ....................................7 1.2.1.5 Minisatellites as Molecular Markers ....................................7 1.2.2 Microsatellites ......................................................................................7 1.2.2.1 Categories of Microsatellites ................................................8 1.2.2.2 Chromosomal Localization and Association with Other Repeats ................................................................................10 1.2.2.3 Mutability and Evolution ....................................................11 1.2.2.4 Microsatellites in Organellar Genomes ..............................13 1.2.2.5 Potential Functions of Microsatellites ................................13 1.2.2.6 Microsatellites as Molecular Markers ................................14 1.3 Transposable Elements ...................................................................................14 1.3.1 Class I Transposons ...........................................................................14 1.3.2 Class II Transposons ..........................................................................17 1.3.3 Unclassified Transposons ...................................................................18 1.3.4 Transposons and Genome Evolution .................................................18 1.3.5 Transposons as Molecular Markers ...................................................19 Chapter 2 Detecting DNA Variation by Molecular Markers 2.1 Properties of Molecular Markers ...................................................................21 2.2 Traditional Marker Systems ...........................................................................22 2.2.1 Protein Markers and Allozymes ........................................................22 2.2.2 DNA Sequencing ...............................................................................23 2.2.3 Restriction Fragment Length Polymorphism (RFLP) Analysis ........24 2.2.3.1 Nuclear RFLPs and DNA Fingerprinting ..........................25 2.2.3.2 RFLPs in Chloroplast and Mitochondrial DNA................26 2.3 The PCR Generation: Molecular Markers Based on In Vitro DNA Amplification ..................................................................................................28 2.3.1 Principle of the PCR ..........................................................................29 2.3.2 Cleaved Amplified Polymorphic Sequences ......................................31 2.3.3 PCR with Arbitrary Primers: RAPD and Its Variants .......................32 2.3.3.1 The Molecular Basis of RAPDs: Significance of Mispriming and Competition among Priming Sites ..........33 2.3.3.2 Properties of RAPD Markers .............................................37 2.3.3.3 Advantages, Limitations, and Applications of RAPD Markers ...............................................................................38 2.3.3.4 Sequence-Characterized Amplified Regions ......................39 2.3.3.5 Expression Profiling with Arbitrary Primers ......................40 2.3.4 Microsatellites ....................................................................................41 2.3.4.1 Nuclear Microsatellite Markers ..........................................42 2.3.4.2 Chloroplast Microsatellite Markers ....................................44 2.3.5 Inter-Repeat PCR ............................................................................... 45 2.3.5.1 From Alu Repeats to Zinc Fingers: Repetitive DNA as a Primer Target ...................................................................45 2.3.5.2 Primers Directed Toward Minisatellites .............................46 2.3.5.3 Primers Directed Toward Microsatellites ...........................47 2.3.5.4 Primers Directed Toward Interspersed Repeats .................52 2.3.6 DNA Profiling of Genie Regions: RGAP, SRAP, and TRAP .........54 2.3.7 Hybridization of Microsatellites to RAPD and MP-PCR Products ... 56 2.3.8 AFLP Analysis and Its Variants .........................................................58 2.3.8.1 The AFLP Technique: Principle, Advantages, and Limitations ..........................................................................58 2.3.8.2 S-SAP Analysis ...................................................................62 2.3.8.3 Selective Amplification of Microsatellite Polymorphic Loci .....................................................................................64 2.3.8.4 Microsatellite-AFLP ...........................................................66 2.3.8.5 Methylation-Sensitive Amplified Polymorphisms ..............66 2.3.8.6 AFLP-Based Expression Profiling .....................................68 2.3.8.7 Miscellaneous AFLP Variants ............................................69 2.3.9 Single-Strand Conformation Polymorphism Analysis and Related Techniques ..........................................................................................70 2.3.10 Miscellaneous Techniques .................................................................72 2.3.10.1 Minisatellite Variant Repeat Mapping ................................72 2.3.10.2 Two-Dimensional DNA Typing Methods ..........................72 2.3.10.3 Single-Nucleotide Polymorphisms .....................................73 Chapter 3 Laboratory Equipment 3.1 Incubators .......................................................................................................75 3.2 Plant and Plant Cell Growth Equipment .......................................................76 3.3 Sterilization ....................................................................................................76 3.4 Water Purification ...........................................................................................76 3.5 Centrifuges .....................................................................................................76 3.6 Refrigeration and Material Storage ...............................................................77 3.7 Safety ............................................................................1... .....!................77 3.8 Pipets ..............................................................................................................77 3.9 Supplies (Glassware and Plasticware) ...........................................................78 3.10 DNA Detection and Quantitation .................................................................78 3.11 Electrophoresis Equipment ............................................................................78 3.12 Documentation of Results ..............................................................................79 3.13 General Laboratory Equipment .....................................................................79 Chapter 4 Methodology 4.1 Safety Precautions ..........................................................................................81 4.2 Isolation, Purification, and Quantitation of Plant DNA................................82 4.2.1 Collection and Preservation of Plant Tissue in the Field ..................82 4.2.1.1 Starting Material .................................................................83 4.2.1.2 Cooling, Freezing, and Lyophilization ...............................84 4.2.1.3 Chemical Preservation ........................................................84 4.2.1.4 Drying .................................................................................85 4.2.1.5 Preparing Herbarium Vouchers ...........................................87 4.2.1.6 Contamination .....................................................................87 4.2.2 Plant DNA Extraction: General Considerations ................................88 4.2.2.1 Cell and Tissue Disruption .................................................89 4.2.2.2 Lysis of Membranes and Organelles ..................................90 4.2.2.3 Removal of Proteins and RNA ...........................................91 4.2.2.4 Removal of Polyphenols and Other Secondary Compounds .........................................................................91 4.2.2.5 Removal of Polysaccharides ...............................................92 4.2.2.6 Removal of Organic Acids and Endogenous DNase Activities .............................................................................92 4.2.2.7 General Strategies to Remove Cytoplasmic Contaminants ......................................................................93 4.2.2.8 Herbarium Specimens and Other Difficult Substrates .......95 4.2.2.9 High-Throughput Procedures .............................................97 4.2.2.10 Commercial Kits .................................................................98 4.2.2.11 Megabase DNA Isolation Protocols ...................................99 4.2.2.12 Choice of Procedure and Costs ..........................................99 4.2.2.13 Storage of DNA Solutions ................................................100 4.2.3 СТАВ Protocol 1 ..............................................................................100 4.2.4 СТАВ Protocol II .............................................................................102 4.2.5 SDS-Potassium Acetate Protocol ....................................................103 4.2.6 DNA Preparation via Nuclei ............................................................104 4.2.7 Quantitation of DNA........................................................................105 4.2.7.1 Ethidium Bromide Staining ..............................................106 4.2.7.2 Spectrophotometry ............................................................106 4.3 Basic Molecular Techniques ........................................................................107 4.3.1 Restriction of DNA..........................................................................107 4.3.2 Polymerase Chain Reaction .............................................................109 4.3.2.1 Primers ..............................................................................110 4.3.2.2 DNA Polymerase ..............................................................110 4.3.2.3 Thermocycler and Temperature Regimen ........................110 4.3.2.4 Template Quality ..............................................................112 4.3.2.5 Yield and Specificity ........................................................112 4.3.2.6 Contamination ...................................................................113 4.3.3 DNA Sequencing .............................................................................114 4.3.4 Agarose Gel Electrophoresis ...........................................................115 4.3.5 PAA Gel Electrophoresis .................................................................118 43.5.1 Nondenaturing PAA Gels .................................................118 4.3.5.2 Sequencing Gels ...............................................................120 4.3.6 Detection of DNA in Gels ...............................................................122 4.3.6.1 Ethidium Bromide Staining ..............................................123 4.3.6.2 Silver Staining ..................................................................123 4.3.7 Gel Drying ........................................................................................124 4.3.8 Southern Blotting .............................................................................125 4.3.9 Generation of Radiolabeled Probes, Primers, and PCR Products.... 127 4.3.9.1 End-Labeling of Oligonucleotides ...................................128 4.3.9.2 Nick Translation ................................................................129 4.3.9.3 Random Priming ...............................................................130 4.3.9.4 Removal of Unincorporated dNTPs .................................131 4.3.9.5 Nonradioactive Labeling Procedures ................................133 4.3.10 Blot Hybridization ............................................................................133 4.3.10.1 Oligonucleotide Probes .....................................................134 4.3.10.2 Probes Generated by Nick Translation or Random Priming ..............................................................................135 4.3.11 Signal Detection ...............................................................................136 4.3.11.1 Autoradiography ...............................................................136 4.3.11.2 Phosphorimaging ..............................................................137 4.3.11.3 Automated DNA Sequencers ............................................137 4.4 PCR with Arbitrary Primers ........................................................................138 4.4.1 Standard RAPD Protocol .................................................................138 4.4.2 Influence of Reaction Conditions and Components ........................139 4.4.2.1 Primers ..............................................................................139 4.4.2.2 Polymerase ........................................................................142 4.4.2.3 Thermocycler and Temperature Regimen ........................142 4.4.2.4 Template Concentration and Quality ................................143 4.4.2.5 Magnesium Concentration ................................................144 4.4.2.6 PCR Additives ...................................................................144 4.4.2.7 Reproducibility and Quality of Banding Patterns ............145 4.4.3 Modifications ....................................................................................146 4.5 Microsatellite-Primed PCR ..........................................................................147 4.5.1 Standard Protocol of Microsatellite-Primed PCR ...........................148 4.5.2 Influence of Reaction Conditions and Components ........................149 4.5.3 Modifications ....................................................................................150 4.5.3.1 Anchored vs. Unanchored Primers ..................................150 4.5.3.2 Fragment Separation and Detection .................................152 4.6 PCR and Hybridization: Combinatory Techniques .....................................152 4.6.1 Assessing the Genomic Copy Number of PCR Amplicons ............153 4.6.2 Testing the Homology of Comigrating Bands ................................153 4.6.3 Random Amplified Microsatellite Polymorphism (RAMPO) .........154 4.7 Amplified Fragment Length Polymorphism ................................................154 4.7.1 Standard AFLP Protocol Using Radioisotopes...............................155 4.7.1.1 Step 1: Restriction of Template DNA and Ligation to Specific Adapters ..............................................................155 4.7.1.2 Step 2: Preamplification ...................................................156 4.7.1.3 Step 3: Selective Amplification ........................................158 4.7.1.4 Step 4: AFLP Fragment Separation and Autoradiography ...............................................................159 4.7.2 AFLP Protocol Using Fluorescence-Labeled Primers ....................159 4.7.2.1 Steps 1 and 2....................................................................160 4.7.2.2 Step 3: Selective Amplification ........................................160 4.7.2.3 Step 4: AFLP Fragment Separation and Fluorescence Detection ...........................................................................161 4.7.3 Selective Amplification of Microsatellite Polymorphic Loci and Microsatellite AFLP Protocols .........................................................162 4.7.4 Technical Aspects and Modifications ..............................................162 4.7.4.1 Restriction and Ligation of Template DNA.....................162 4.7.4.2 Preamplification and Selective Amplification ..................165 4.7.4.3 Fragment Separation and Detection .................................167 4.7.5 Robustness and Reproducibility ......................................................169 4.8 Generation and Analysis of Microsatellite Markers ....................................170 4.8.1 Microsatellite Analysis Using Radioisotopes..................................171 4.8.2 Microsatellite Analysis Using Fluorochromes ................................174 4.8.3 Technical Aspects and Modifications ..............................................176 4.8.3.1 Fragment Separation and Visualization ............................176 4.8.3.2 Stutter Bands and Other PCR Artefacts ...........................177 4.8.4 Generating Microsatellite Markers Without Cloning ......................178 4.8.4.1 Literature Screening ..........................................................178 4.8.4.2 Database Mining ...............................................................179 4.8.4.3 Marker Transferability between Species: Nuclear Microsatellites ...................................................................180 4.8.4.4 Marker Transferability between Species: Chloroplast Microsatellites................................................................... 182 4.8.5 Microsatellite Cloning ......................................................................183 4.8.5.1 Conventional Libraries ......................................................183 4.8.5.2 Microsatellite Enrichment Based on Primer Extension... 184 4.8.5.3 Microsatellite Enrichment Based on Selective Hybridization ....................................................................185 4.8.5.4 Microsatellites from Cloned PCR Products .....................187 4.8.5.5 Miscellaneous Enrichment Procedures .............................188 4.8.5.6 Protocol: Microsatellite Enrichment Cloning Using Magnetic Beads ................................................................189 4.8.5.7 Primer Design ...................................................................199 4.8.5.8 Factors Affecting the Efficiency of Microsatellite Cloning ..............................................................................200 4.8.5.9 Commercial Development of Microsatellite Libraries ....202 4.9 CAPS Analysis of cpDNA and mtDNA ......................................................202 4.9.1 Standard CAPS Protocol ..................................................................203 4.9.2 Choice of CAPS Primers .................................................................205 Chapter 5 Evaluation of Molecular Marker Data 5.1 Robustness and Reproducibility ..................................................................207 5.1.1 Reliability .........................................................................................208 5.1.2 BandHomology ...............................................................................208 5.1.3 Band Linkage and Neutrality ...........................................................209 5.2 Fragment Sizing and Matching ...................................................................210 5.2.1 General Precautions .........................................................................211 5.2.2 Equipment ........................................................................................212 5.3 Multilocus vs. Single-Locus Approaches ....................................................213 5.3.1 Multilocus Markers ..........................................................................213 5.3.2 Single-Locus Markers and Polyploids .............................................213 5.4 Band Sharing and Genetic Distances ..........................................................214 5.4.1 Coefficients of Similarity .................................................................214 5.4.2 Dissimilarity Coefficients and Genetic Distances ...........................215 5.4.3 Identity and Uniqueness ...................................................................215 5.4.4 Clonal Structure ...............................................................................217 5.5 Ordination, Clustering, and Dendrograms ...................................................219 5.5.1 Ordination Techniques .....................................................................219 5.5.2 Construction of Dendrograms ..........................................................221 5.6 Population Genetic Analysis ........................................................................223 5.6.1 Measures of Variation ......................................................................223 5.6.2 Genetic Differentiation between Populations ..................................226 5.6.2.1 F Statistics and Related Measures ....................................226 5.6.2.2 Analysis of Variance .........................................................228 5.6.2.3 Shannon s Index ................................................................228 5.6.3 Genetic Distances between Populations ..........................................229 5.6.4 Inbreeding Coefficient and Mating Systems ...................................229 5.6.5 Estimation of Relatedness and Paternity Testing ............................230 5.6.6 Migration and Hybridization ...........................................................231 5.6.7 Gene Flow, Isolation-by-Distance, and Spatial Structure ...............232 5.7 Phylogeography and Nested Clade Analysis ...............................................232 5.8 Statistical Testing of Hypotheses: Analytical and Computational Methods ... 233 Chapter б Applications of DNA Fingerprinting in Plant Sciences 6.1 A Brief History of DNA Fingerprinting ......................................................235 6.1.1 Minisatellite and Oligonucleotide DNA Probes Detect Genetic Variation .................................................. .....235 6.1.2 PCR-Based Methods Enter the Stage ..............................................236 6.1.3 Microsatellite DNA Analyses Yield Codominant Markers .............237 6.1.4 Universal Organellar DNA Primers Produce Uniparental Markers .............................................................................................237 6.2 Genotype Identification ................................................................................237 6.2.1 Individual-Specific DNA Fingerprints .............................................238 6.2.2 Cultivar Identification ......................................................................240 6.2.2.1 Patenting and Protecting Plant Varieties ..........................240 6.2.2.2 Choice of DNA Marker Method for Cultivar Identification .....................................................................241 6.2.2.3 Propagation and Reproduction of Crop Species ..............242 6.2.3 In V/rro-Propagated Plant Material and Somaclonal Variation .......243 6.2.4 Sports and Other Mutants ................................................................245 6.3 Genetic Diversity .........................................................................................246 6.3.1 Variation and Relatedness among Cultivars ....................................247 6.3.2 Analysis of Population Genetic Diversity and Its Distribution ......248 6.3.2.1 Choice of Molecular Marker Method for Population Genetics .............................................................................249 6.3.2.2 Influence of the Breeding System on Genetic Diversity ............................................................................250 6.3.2.3 Clones and Ramets ...........................................................251 6.3.2.4 Estimating Gene Flow via Pollen and Seeds ...................252 6.3.2.5 Effects of Habitat Fragmentation .....................................255 6.3.3 Hybridization and Introgression ......................................................257 6.3.3.1 Hybridization in Wild Populations ...................................257 6.3.3.2 Hybrid Distances and Diagnostic Markers ......................259 6.3.3.3 Hybridization between Wild and Cultivated Plants .........259 6.3.3.4 Hybridization in Gymnosperms .......................................260 6.3.3.5 Polyploidy .........................................................................260 6.3.4 Plant Conservation ...........................................................................261 6.3.5 Germplasm Characterization and Preservation ...............................262 6.3.5.1 Gene Banks .......................................................................263 6.3.5.2 Core Collections ...............................................................264 6.4 Plant Taxonomy and Systematics ................................................................264 6.4.1 Taxonomie Relationships Revealed by Multilocus DNA Methods ............................................................................................264 6.4.2 Microsatellite Markers in Taxonomie Studies .................................268 6.4.3 Taxonomie Consequences from DNA Profiling Data .....................269 6.5 Phylogeography ............................................................................................270 6.5.1 Phylogeography Based on cpDNA ..................................................270 6.5.1.1 Postglacial Recolonization of Central and Northern Europe ...............................................................................271 6.5.1.2 Phylogeographic Case Studies in Tropical Trees .............271 6.5.1.3 Phylogeographic Case Studies in Gymnosperms ............272 6.5.2 Phylogeography Based on Nuclear Genes ......................................274 Chapter 7 Linkage Analysis and Genetic Maps 7.1 Generating High-Density Genetic Maps .....................................................277 7.1.1 Selection of Parent Plants ................................................................277 7.1.2 Mapping Population .........................................................................278 7.1.3 Linkage Analysis ..............................................................................279 7.1.4 The Genetic Map .............................................................................279 7.1.5 Cytogenetic Maps ............................................................................285 7.1.6 Genetic vs. Physical Maps ...............................................................285 7.2 Synteny: The Comparative Analysis of Genomes .......................................287 7.3 Marker-Assisted Selection ...........................................................................288 7.4 Molecular Markers and Positional Cloning .................................................289 Chapter 8 Which Marker for What Purpose: A Comparison 8.1 Morphological Characters and Allozymes vs. DNA Markers ....................293 8.2 Different Kinds of DNA Markers ................................................................295 8.2.1 Discriminatory Power ......................................................................295 8.2.2 Genetic Distances .............................................................................296 8.2.3 Within- and Among-Population Variation .......................................297 8.2.4 Gene Tagging and Genetic Linkage Mapping .................................297 8.2.5 Costs .................................................................................................298 8.3 Conclusions ..................................................................................................298 Chapter 9 Future Prospects: SNiPs and Chips for DNA and RNA Profiling 9.1 Single-Nucleotide Polymorphisms ..............................................................301 9.1.1 What Is a SNiP? ...............................................................................301 9.1.2 SNP Discovery .................................................................................302 9.1.3 SNP Genotyping ..............................................................................303 9.1.4 SNPs in Plant Genomes ...................................................................304 9.1.5 Perspective ........................................................................................305 9.2 DNA Microarrays .........................................................................................305 9.3 Expression Profiling and Expression Markers ............................................308 Appendix 1 Plant DNA Isolation Protocols ...........................................................................311 Appendix 2 Commercial Companies ......................................................................................323 Appendix 3 Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data ............................................329 Appendix 4 Web Pages of Interest ..........................................................................................337 References ............................................................................................................339 Index ......................................................................................................................427
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id	DE-604.BV019414749
illustrated	Illustrated
indexdate	2024-07-09T19:59:47Z
institution	BVB
isbn	0849314887
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-012876592
oclc_num	57251282
open_access_boolean
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owner_facet	DE-703 DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-29T DE-11 DE-188
physical	444 S. Ill., graph. Darst.
publishDate	2005
publishDateSearch	2005
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publisher	Taylor & Francis
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spelling	DNA fingerprinting in plants principles, methods, and applications Kurt Weising ... 2. ed. Boca Raton [u.a.] Taylor & Francis 2005 444 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier 1. Auf. u.d.T.: DNA fingerprinting in plants and fungi Dna larpcal Genética vegetal larpcal Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of plants Plants genetics Genetischer Fingerabdruck (DE-588)4245149-8 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Pilze (DE-588)4046076-9 gnd rswk-swf Fingerprint-Verfahren (DE-588)4231579-7 gnd rswk-swf Pflanzen (DE-588)4045539-7 s Genetischer Fingerabdruck (DE-588)4245149-8 s DE-604 Pilze (DE-588)4046076-9 s Fingerprint-Verfahren (DE-588)4231579-7 s Weising, Kurt Sonstige oth 1. Auflage DNA fingerprinting in plants and fungi (DE-604)BV010329307 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012876592&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	DNA fingerprinting in plants principles, methods, and applications Dna larpcal Genética vegetal larpcal Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of plants Plants genetics Genetischer Fingerabdruck (DE-588)4245149-8 gnd Pflanzen (DE-588)4045539-7 gnd Pilze (DE-588)4046076-9 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd
subject_GND	(DE-588)4245149-8 (DE-588)4045539-7 (DE-588)4046076-9 (DE-588)4231579-7
title	DNA fingerprinting in plants principles, methods, and applications
title_auth	DNA fingerprinting in plants principles, methods, and applications
title_exact_search	DNA fingerprinting in plants principles, methods, and applications
title_full	DNA fingerprinting in plants principles, methods, and applications Kurt Weising ...
title_fullStr	DNA fingerprinting in plants principles, methods, and applications Kurt Weising ...
title_full_unstemmed	DNA fingerprinting in plants principles, methods, and applications Kurt Weising ...
title_old	DNA fingerprinting in plants and fungi
title_short	DNA fingerprinting in plants
title_sort	dna fingerprinting in plants principles methods and applications
title_sub	principles, methods, and applications
topic	Dna larpcal Genética vegetal larpcal Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of plants Plants genetics Genetischer Fingerabdruck (DE-588)4245149-8 gnd Pflanzen (DE-588)4045539-7 gnd Pilze (DE-588)4046076-9 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd
topic_facet	Dna Genética vegetal Plantes - Empreintes génétiques DNA Fingerprinting DNA fingerprinting of plants Plants genetics Genetischer Fingerabdruck Pflanzen Pilze Fingerprint-Verfahren
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012876592&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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