Cell cycle control and dysregulation protocols: cyclins, cyclin-dependent kinases, and other factors
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2004
|
| Schriftenreihe: | Methods in molecular biology
285 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIII, 185 S. Ill., graph. Darst. |
| ISBN: | 0896039498 |
Internformat
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| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2004 | |
| 300 | |a XIII, 185 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 285 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Celbiologie |2 gtt | |
| 650 | 7 | |a Celcyclus |2 gtt | |
| 650 | 4 | |a Cycle cellulaire - Manuels de laboratoire | |
| 650 | 4 | |a Cyclines - Manuels de laboratoire | |
| 650 | 7 | |a Genexpressie |2 gtt | |
| 650 | 7 | |a Kinasen |2 gtt | |
| 650 | 4 | |a Kinases dépendantes des cyclines - Manuels de laboratoire | |
| 650 | 4 | |a Cell Cycle Proteins |x genetics | |
| 650 | 4 | |a Cell cycle |v Laboratory manuals | |
| 650 | 4 | |a Cyclin-Dependent Kinases | |
| 650 | 4 | |a Cyclin-dependent kinases |v Laboratory manuals | |
| 650 | 4 | |a Cyclins |v Laboratory manuals | |
| 650 | 4 | |a DNA | |
| 650 | 4 | |a Fluorescent Antibody Technique | |
| 650 | 4 | |a Gene Expression | |
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Datensatz im Suchindex
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| adam_text | Contents
Preface v
Contributors xi
Part I. Analysis of Cyclins and Cyclin Dependent Kinases
1 The Biology of Cyclins and Cyclin Dependent Protein Kinases:
An Introduction
Lucio Miele 3
2 In Situ Immunofluorescence Analysis:
Immunofluorescence Microscopy
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher f. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 23
3 In Situ Immunofluorescence Analysis: Analyzing RNA Synthesis
by 5 Bromouridine 5 Triphosphate Labeling
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 29
4 Immunofluorescence Analysis Using Epitope Tagged Proteins:
In Vitro System
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 33
5 Analysis of In Vivo Gene Expression Using Epitope Tagged Proteins
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 37
vii
viii Contents
6 Chromatin Immunoprecipitation
Amjad laved, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 41
7 Protein Deoxyribonucleic Acid Interactions Linked to Gene
Expression: Electrophoretic Mobility Shift Assay
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 45
8 Protein Deoxyribonucleic Acid Interactions Linked to Gene
Expression: DNase I Digestion
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 57
9 Protein Deoxyribonucleic Acid Interactions Linked to Gene
Expression: Ligation Mediated Polymerase Chain Reaction
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez,
Christopher J. Lengner, Kimberly S. Harrington,
Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap,
Shirwin M. Pockwinse, Martin Montecino,
Andre J. van Wijnen, Jane B. Lian, Janet L. Stein,
and Gary S. Stein 63
10 Assays for Cyclin Dependent Kinase Inhibitors
Adrian M. Senderowicz 69
11 Protein Degradation Via the Proteosome
Henry Hoff, Hong Zhang, and Christian Sell 79
Part II. Analysis of the Factors Involved in CellCycle Deregulation
12 The Transformed Phenotype
Henry Hoff, Barbara Belletti, Hong Zhang,
and Christian Sell 95
Contents ix
13 A Morphologic Approach to Detect Apoptosis Based
on Electron Microscopy
Martyn K. White and Caterina Cinti 105
14 Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ
Nick Translation
Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli,
and Caterina Cinti 113
Part HI. Cellular Response to Deoxyribonucleic Acid Damage
15 Induction of Deoxyribonucleic Acid Damage by Alkylating Agents
Salvatore Cortellino, David P. Turner, Domenico Albino,
and Alfonso Bellacosa 121
16 Induction of Deoxyribonucleic Acid Damage by y Irradiation
Salvatore Cortellino, David P. Turner
and Alfonso Bellacosa 127
17 Ultraviolet Irradiation of Cells
David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa 133
Part IV. Retroviridae Based Vectors: Protocols
for Lentiviral and Retroviral Mediated Gene Transfer
to Engineer Cell Culture Systems
18 Transient Production of Retroviral and Lentiviral Based Vectors
for the Transduction of Mammalian Cells
Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano,
and Gaetano Romano 141
19 Determination of Functional Viral Titer by Drug Resistance
Colony Assay, Expression of Green Fluorescent Protein,
and p Galactoside Staining
Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano,
and Gaetano Romano 149
20 Retroviral and Lentiviral Vector Titration by the Analysis
of the Activity of Viral Reverse Transcriptase
Tiziana Tonini, Pier Paolo Claudio, Antonio Giordano,
and Gaetano Romano 155
Part V. Detection of Gene Expression in Subcellular Compartments
21 Single and Double Colloidal Gold Labeling in Postembedding
Immunoelectron Microscopy
Nicoletta Zini, Liliana Solimando, Caterina Cinti,
and Nadir Mario Maraldi 161
x Contents
22 Multifluorescence Labeling and Colocalization Analyses
Massimo Riccio, Maja Dembic, Caterina Cinti,
and Spartaco Santi 171
Index 179
|
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| dewey-ones | 571 - Physiology & related subjects |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| spelling | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors edited by Antonio Giordano ... Totowa, N.J. Humana Press 2004 XIII, 185 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 285 Includes bibliographical references and index Celbiologie gtt Celcyclus gtt Cycle cellulaire - Manuels de laboratoire Cyclines - Manuels de laboratoire Genexpressie gtt Kinasen gtt Kinases dépendantes des cyclines - Manuels de laboratoire Cell Cycle Proteins genetics Cell cycle Laboratory manuals Cyclin-Dependent Kinases Cyclin-dependent kinases Laboratory manuals Cyclins Laboratory manuals DNA Fluorescent Antibody Technique Gene Expression Labortechnik (DE-588)4123602-6 gnd rswk-swf Zellzyklus (DE-588)4129960-7 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf Cyclin-abhängige Kinasen (DE-588)4485596-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Cyclin-abhängige Kinasen (DE-588)4485596-5 s Labortechnik (DE-588)4123602-6 s DE-604 Zellzyklus (DE-588)4129960-7 s Regulation (DE-588)4049075-0 s b DE-604 Giordano, Antonio Sonstige oth Methods in molecular biology 285 (DE-604)BV035362695 285 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012834312&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors Methods in molecular biology Celbiologie gtt Celcyclus gtt Cycle cellulaire - Manuels de laboratoire Cyclines - Manuels de laboratoire Genexpressie gtt Kinasen gtt Kinases dépendantes des cyclines - Manuels de laboratoire Cell Cycle Proteins genetics Cell cycle Laboratory manuals Cyclin-Dependent Kinases Cyclin-dependent kinases Laboratory manuals Cyclins Laboratory manuals DNA Fluorescent Antibody Technique Gene Expression Labortechnik (DE-588)4123602-6 gnd Zellzyklus (DE-588)4129960-7 gnd Regulation (DE-588)4049075-0 gnd Cyclin-abhängige Kinasen (DE-588)4485596-5 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4129960-7 (DE-588)4049075-0 (DE-588)4485596-5 (DE-588)4143413-4 |
| title | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors |
| title_auth | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors |
| title_exact_search | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors |
| title_full | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors edited by Antonio Giordano ... |
| title_fullStr | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors edited by Antonio Giordano ... |
| title_full_unstemmed | Cell cycle control and dysregulation protocols cyclins, cyclin-dependent kinases, and other factors edited by Antonio Giordano ... |
| title_short | Cell cycle control and dysregulation protocols |
| title_sort | cell cycle control and dysregulation protocols cyclins cyclin dependent kinases and other factors |
| title_sub | cyclins, cyclin-dependent kinases, and other factors |
| topic | Celbiologie gtt Celcyclus gtt Cycle cellulaire - Manuels de laboratoire Cyclines - Manuels de laboratoire Genexpressie gtt Kinasen gtt Kinases dépendantes des cyclines - Manuels de laboratoire Cell Cycle Proteins genetics Cell cycle Laboratory manuals Cyclin-Dependent Kinases Cyclin-dependent kinases Laboratory manuals Cyclins Laboratory manuals DNA Fluorescent Antibody Technique Gene Expression Labortechnik (DE-588)4123602-6 gnd Zellzyklus (DE-588)4129960-7 gnd Regulation (DE-588)4049075-0 gnd Cyclin-abhängige Kinasen (DE-588)4485596-5 gnd |
| topic_facet | Celbiologie Celcyclus Cycle cellulaire - Manuels de laboratoire Cyclines - Manuels de laboratoire Genexpressie Kinasen Kinases dépendantes des cyclines - Manuels de laboratoire Cell Cycle Proteins genetics Cell cycle Laboratory manuals Cyclin-Dependent Kinases Cyclin-dependent kinases Laboratory manuals Cyclins Laboratory manuals DNA Fluorescent Antibody Technique Gene Expression Labortechnik Zellzyklus Regulation Cyclin-abhängige Kinasen Aufsatzsammlung |
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