Receptor signal transduction protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
259 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 418 S. Ill., graph. Darst. |
| ISBN: | 1588293297 |
Internformat
MARC
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| 245 | 1 | 0 | |a Receptor signal transduction protocols |c ed. by Gary B. Willars ... |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2004 | |
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| 650 | 4 | |a Transduction du signal cellulaire - Recherche - Méthodologie | |
| 650 | 4 | |a Cell receptors |x Research |x Methodology | |
| 650 | 4 | |a Cellular signal transduction |x Research |x Methodology | |
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Datensatz im Suchindex
| _version_ | 1804132803053355008 |
|---|---|
| adam_text | Contents
Preface v
Preface to the First Edition vi
Contributors xi
1 Radioligand Binding Methods for Membrane Preparations
and Intact Cells
David B. Bylund, Jean D. Deupree, and Myron L. Toews 1
2 Determination of Allosteric Interactions
Using Radioligand Binding Techniques
Sebastian Lazareno 29
3 Generation, Use, and Validation
of Receptor Selective Antibodies
John J. Mackrill 47
4 Immunocytochemical Identification of G Protein Coupled
Receptor Expression and Localization
Laura C. Mongan and Blair D. Grubb 67
5 Generation and Use of Epitope Tagged Receptors
R. A. Jeffrey Mcllhinney 81
6 Identification of G Protein Coupled Receptor mRNA
Expression by Northern Blotting and In Situ Hybridization
Lucy F. Donaldson 99
7 Electron Microscopic Studies of Receptor Localization
Rafael Lujan 123
8 Generation of Model Cell Lines
Expressing Recombinant G Protein Coupled Receptors
Emmanuel Hermans 137
9 Viral Infection Protocols
Antonio Porcellini and Antonio De Blasi 755
10 Expression of G Protein Signaling Components
in Adult Mammalian Neurons by Microinjection
Stephen R. Ikeda 167
vii
viii Contents
11 Covalent Modification of G Proteins by Affinity Labeling
Martin Hohenegger, Michael Freissmuth,
and Christian Nanoff 183
12 Measurement of Agonist Stimulated [35S]GTPyS Binding
to Assess Total G Protein and Ga Subtype Specific
Activation by G Protein Coupled Receptors
Mark R. Dowling, Stefan R. Nahorski,
and R. A. John Challiss 197
13 Identification and Quantitation of G Protein a Subunits
Ian Mullaney and Graeme Milligan 207
14 Analysis of Function of Receptor G Protein
and Receptor RGS Fusion Proteins
Richard J. Ward and Graeme Milligan 225
15 Assessment of Receptor Internalization and Recycling
Jennifer A. Koenig 249
16 G Protein Coupled Receptor Phosphorylation
and Palmitoylation
Andrew B. Tobin and Mark Wheatley 275
1 7 Identification of G Protein Coupled Receptor
Phosphorylation Sites by 2D Phosphopeptide Mapping
Andree Blaukat 283
18 Ubiquitination of G Protein Coupled Receptors
Adriano Marchese and Jeffrey L. Benovic 299
19 Receptor Mutagenesis Strategies for Examination
of Structure Function Relationships
Marion Blomenrohr, Henry F. Vischer, and Jan Bogerd 307
20 Study of G Protein Coupled Receptor Protein Interactions
by Bioluminescence Resonance Energy Transfer
Karen M. Kroeger and Karin A. Eidne 323
21 Fluorescence Resonance Energy Transfer
to Study Receptor Dimerization in Living Cells
Jiirgen F. Bader and Annette G. Beck Sickinger 335
22 Identification of Protein Interactions by Yeast Two Hybrid
Screening and Coimmunoprecipitation
Michael Tanowitz and Mark von Zastrow 353
Contents ix
23 Study of G Protein Coupled Receptor Protein Interactions
Using Gel Overlay Assays and
Glutathione S Transferase Fusion Protein Pull Downs
Ashley E. Brady, Qin Wang, and Lee E. Limbird 371
24 Receptor Knock Out and Knock In Strategies
Ikuo Matsuda and Atsu Aiba 379
25 Statistical Methods in G Protein Coupled Receptor Research
Pat Freeman and Domenico Spina 391
Index 415
|
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| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| spelling | Receptor signal transduction protocols ed. by Gary B. Willars ... 2. ed. Totowa, NJ Humana Press 2004 XIII, 418 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 259 Signal Transduction cabt Cell Receptors cabt Laboratory Manuals cabt Récepteurs cellulaires - Recherche - Méthodologie Transduction du signal cellulaire - Recherche - Méthodologie Cell receptors Research Methodology Cellular signal transduction Research Methodology Signal Transduction physiology Laboratory Manuals Rezeptor (DE-588)4049722-7 gnd rswk-swf Zellrezeptor (DE-588)4133853-4 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Rezeptor (DE-588)4049722-7 s Signaltransduktion (DE-588)4318717-1 s Labortechnik (DE-588)4123602-6 s DE-604 Zellrezeptor (DE-588)4133853-4 s Methode (DE-588)4038971-6 s 1\p DE-604 2\p DE-604 Willars, Gary B. Sonstige oth Methods in molecular biology 259 (DE-604)BV035362695 259 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012829961&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Receptor signal transduction protocols Methods in molecular biology Signal Transduction cabt Cell Receptors cabt Laboratory Manuals cabt Récepteurs cellulaires - Recherche - Méthodologie Transduction du signal cellulaire - Recherche - Méthodologie Cell receptors Research Methodology Cellular signal transduction Research Methodology Signal Transduction physiology Laboratory Manuals Rezeptor (DE-588)4049722-7 gnd Zellrezeptor (DE-588)4133853-4 gnd Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4049722-7 (DE-588)4133853-4 (DE-588)4123602-6 (DE-588)4318717-1 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Receptor signal transduction protocols |
| title_auth | Receptor signal transduction protocols |
| title_exact_search | Receptor signal transduction protocols |
| title_full | Receptor signal transduction protocols ed. by Gary B. Willars ... |
| title_fullStr | Receptor signal transduction protocols ed. by Gary B. Willars ... |
| title_full_unstemmed | Receptor signal transduction protocols ed. by Gary B. Willars ... |
| title_short | Receptor signal transduction protocols |
| title_sort | receptor signal transduction protocols |
| topic | Signal Transduction cabt Cell Receptors cabt Laboratory Manuals cabt Récepteurs cellulaires - Recherche - Méthodologie Transduction du signal cellulaire - Recherche - Méthodologie Cell receptors Research Methodology Cellular signal transduction Research Methodology Signal Transduction physiology Laboratory Manuals Rezeptor (DE-588)4049722-7 gnd Zellrezeptor (DE-588)4133853-4 gnd Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Signal Transduction Cell Receptors Laboratory Manuals Récepteurs cellulaires - Recherche - Méthodologie Transduction du signal cellulaire - Recherche - Méthodologie Cell receptors Research Methodology Cellular signal transduction Research Methodology Signal Transduction physiology Laboratory Manuals Rezeptor Zellrezeptor Labortechnik Signaltransduktion Methode Aufsatzsammlung |
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