Bacterial artificial chromosomes: 1 Library construction, physical mapping, and sequencing
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N. J.
Humana Press
2004
|
| Schriftenreihe: | Methods in molecular biology
255 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 360 S. Ill., graph. Darst. |
| ISBN: | 0896039889 |
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Datensatz im Suchindex
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| adam_text | Contents of Volume 1
Library Construction, Physical Mapping, and Sequencing
Preface v
Contents of the Companion Volume xiii
Contributors xv
1 BAC Library Construction
Kazutoyo Osoegawa and Pieter ). de Jong 1
2 Construction of Small Genome BAC Library for Functional
and Cenomic Applications
David Smajs, Steven ]. Norris, and George M. Weinstock 47
3 Preparation of BAC Libraries From Bacterial Genomes
by In Vitro Packaging
Sangita Pal, Solida Mak, and George M. Weinstock 57
4 Exploring Transformation Associated Recombination Cloning
for Selective Isolation of Genomic Regions
Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine,
Sun Hee Leem, and Vladimir Larionov 69
5 Purification of BAC DNA
Tim S. Poulsen 91
6 Hybridization Based Selection of BAC Clones
Chang Su Lim and Ung )in Kim 101
7 Applications of Interspersed Repeat Sequence
Polymerase Chain Reaction
Heike Zimdahl, Claudia Gbsele, Thomas Kreitler,
and Margit Knoblauch 113
8 BAC Mapping Using Fluorescence In Situ Hybridization
Xiao Ning Chen and Julie R. Korenberg 131
9 High Throughput BAC Fingerprinting
Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon,
Duane Smailus, Robert Waterston, and Marco Marra 143
10 BAC End Sequencing
Tim S. Poulsen and Hans E. lohnsen 157
ix
x Contents
11 Radiation Hybrid Mapping With BAC Ends
Michael Olivier, Shannon Brady, and David R. Cox 163
12 Shotgun Library Construction for DNA Sequencing
Bruce A. Roe 171
1 3 Rolling Circle Amplification for Sequencing Templates
Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett,
Susan Lucas, Tijana Glavina, and Trevor Hawkins 189
14 Transposon Mediated Sequencing
Rachel Reeg and Anup Madan 197
15 Pyrosequencing: A Tool for DNA Sequencing Analysis
Elahe Elahi and Mostafa Ronaghi 211
16 Use of Fimers to Eliminate Polymerase Chain Reaction
and Primer Dimer Artifacts and to Increase Yield
in BAC Sequencing Reactions
Andrei Malykh, Nikolai Polushin, Alexei Slesarev,
and Sergei Kozyavkin 221
1 7 Retrofitting BACs and PACs With LoxP Transposons
to Generate Nested Deletions
Pradeep K. Chatterjee 231
18 Preparing Nested Deletion Template DNA for Field Inversion Gel
Electrophoresis Analyses and Position Specific End Sequencing
With Transposon Primers
Pradeep K. Chatterjee and Joseph C. Baker, r 243
19 BAC Finishing Strategies
Christine Bird and Darren Grafham 255
20 Using the TIGR Assembler in Shotgun Sequencing Projects
Mihai Pop and Dan Kosack 279
21 Finishing Working Draft BAC Projects by Directed Sequencing
With ThermoFidelase and Fimers
Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin,
and Alexei Slesarev 295
22 Optimized Multiplex Polymerase Chain Reaction:
An Effective Method for Rapid Gap Closure
Diana Radune and Herve Tettelin 309
23 Assembly of DNA Sequencing Data
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers 319
Contents xi
24 Sequence Finishing
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers 333
25 Quality Assessment of Finished BAC Sequences
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers 343
Index 351
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T19:58:37Z |
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| isbn | 0896039889 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Bacterial artificial chromosomes 1 Library construction, physical mapping, and sequencing Ed. by Shaying Zhao ... Totowa, N. J. Humana Press 2004 XX, 360 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 255 Methods in molecular biology ... Künstliches Chromosom (DE-588)4767094-0 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Bakterien (DE-588)4004296-0 s Künstliches Chromosom (DE-588)4767094-0 s Labortechnik (DE-588)4123602-6 s DE-604 Zhao, Shaying Sonstige oth (DE-604)BV019366253 1 Methods in molecular biology 255 (DE-604)BV035362695 255 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012829874&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bacterial artificial chromosomes Methods in molecular biology Künstliches Chromosom (DE-588)4767094-0 gnd Bakterien (DE-588)4004296-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4767094-0 (DE-588)4004296-0 (DE-588)4123602-6 |
| title | Bacterial artificial chromosomes |
| title_auth | Bacterial artificial chromosomes |
| title_exact_search | Bacterial artificial chromosomes |
| title_full | Bacterial artificial chromosomes 1 Library construction, physical mapping, and sequencing Ed. by Shaying Zhao ... |
| title_fullStr | Bacterial artificial chromosomes 1 Library construction, physical mapping, and sequencing Ed. by Shaying Zhao ... |
| title_full_unstemmed | Bacterial artificial chromosomes 1 Library construction, physical mapping, and sequencing Ed. by Shaying Zhao ... |
| title_short | Bacterial artificial chromosomes |
| title_sort | bacterial artificial chromosomes library construction physical mapping and sequencing |
| topic | Künstliches Chromosom (DE-588)4767094-0 gnd Bakterien (DE-588)4004296-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Künstliches Chromosom Bakterien Labortechnik |
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