Bacterial and eukaryotic porins: structure, function, mechanism
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| Format: | Buch |
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| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 366 S. Ill., graph. Darst. |
| ISBN: | 3527307753 |
Internformat
MARC
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| 008 | 040810s2004 gw ad|| |||| 00||| eng d | ||
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| 020 | |a 3527307753 |c Gb. : ca. EUR 139.00 (freier Pr.), ca. CHF 205.00 (freier Pr.) |9 3-527-30775-3 | ||
| 035 | |a (OCoLC)56515197 | ||
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| 245 | 1 | 0 | |a Bacterial and eukaryotic porins |b structure, function, mechanism |c ed. by Roland Benz |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2004 | |
| 300 | |a XX, 366 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Bacteriologia médica |2 larpcal | |
| 650 | 7 | |a Biologia celular |2 larpcal | |
| 650 | 4 | |a Molécules | |
| 650 | 7 | |a Proteínas (estrutura) |2 larpcal | |
| 650 | 4 | |a Protéines - Structure | |
| 650 | 4 | |a Eukaryotic Cells |x physiology | |
| 650 | 4 | |a Molecules | |
| 650 | 4 | |a Porins |x physiology | |
| 650 | 4 | |a Proteins |x Structure | |
| 650 | 0 | 7 | |a Porin |0 (DE-588)4221584-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Porin |0 (DE-588)4221584-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Benz, Roland |d 1943-2025 |e Sonstige |0 (DE-588)129268933 |4 oth | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012824169&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-012824169 | |
Datensatz im Suchindex
| _version_ | 1823116780755746816 |
|---|---|
| adam_text |
Contents
Preface XIV
List of Contributors XVIII
1 Regulation of Porin Gene Expression by the Two component
Regulatory System EnvZ/OmpR 1
Don Walthers, Alvin Go and Linda J. Kenney
1.1 Introduction 1
1.2 The Structure of EnvZ 2
1.3 Biochemical Activities of EnvZ underlie Signaling 4
1.4 What is the EnvZ Activity Regulated by the Stimulus? 5
1.5 How is the Signal Propagated? 8
1.6 Is there a Role for Acetyl phosphate in OmpR P Production? 9
1.7 The OmpR Subfamily 9
1.8 OmpR Binding Sites 11
1.9 Recruitment of RNA Polymerase to OmpR dependent Promoters 13
1.10 OmpR RNAP Interaction Surface 14
1.11 Affinity Model of Porin Gene Regulation 15
1.12 A Test of the Affinity Model 16
1.13 Conformational Changes in OmpR Contribute to
Differential Regulation of the Porin Genes 17
1.14 Other Factors that Regulate ompF and ompC 18
1.15 OmpR is a Global Regulator 19
1.15.1 Flagellar Biosynthesis 20
1.15.2 Curli Fimbriae Production 20
1.15.3 Virulence 21
Acknowledgments 21
References 22
Bacterial and Eukaryotic Porins. Edited by Roland Benz
Copyright © 2004 WILEY VCH Verlag GmbH Co. KGaA, Weinheim
ISBN 3 527 30775 3
VI I Contents
2 The Structures of General Porins 25
Ge.org E. Schulz
2.1 Bacterial Outer Membrane Proteins 25
2.2 Construction of General Porins 29
2.3 Trimer Association and Folding 33
2.4 Pore Geometry 34
2.5 Permeation 36
2.6 Conclusion 37
Acknowledgments 38
References 38
3 Role of Bacterial Porins in Antibiotic Susceptibility of
Cram negative Bacteria 41
Jean Marie Pages
3.1 Introduction 41
3.2 Role of Porins in Antibiotic Resistance 42
3.2.1 Evolution of Clinical Isolates 45
3.2.2 Expression of a Modified Porin 48
3.3 In Vitro Mutagenesis Analyses of Porins and Modeling 53
3.3.1 Mutations in the Loop 3 Domain 52
3.3.2 Mutations in the Anti loop 3 Domain 53
3.3.3 Modeling of P Lactam in the OmpF Eyelet 53
3.4 Conclusion 54
Acknowledgments 55
References 55
4 Porins of the Outer Membrane of Pseudomonas aeruginosa 61
Robert E. W. Hancock and Sandeep Tamber
4.1 Introduction 63
4.2 The Outer Membrane Permeability Defect in P. aeruginosa 62
4.3 Porins Identified in the Genome Sequence 63
4.4 The General Porins 64
4.4.1 OprF 65
4.4.2 Other General Porins 67
4.5 Efflux 67
4.5.1 OprM 68
4.5.2 OprM Homologs 68
4.6 Specific Porins 69
4.6.1 OprB 70
4.6.2 OprP and OprO 70
4.6.3 OprD 73
4.6.4 OprD Homologs 71
4.7 TonB dependent Receptors 72
4.7.1 FpvA 73
4.7.2 FptA 73
Contents I VII
4.7.3 PfeA and PirA 73
4.7.4 HasR, PhuR and OptI 74
4.7.5 Other TonB dependent Receptors 74
4.8 Conclusions 74
Acknowledgments 75
References 75
5 Regulation of Bacterial Porin Function 79
Arnaud Basle and Anne H. Delcour
5.1 Introduction 79
5.2 Voltage Dependence 80
5.2.1 L3 and the Constriction Zone 82
5.2.2 Extracellular Loops 85
5.2.3 Modulation of Voltage Gating 86
5.3 Effect of pH 87
5.4 Polyamine Modulation 88
5.5 Others 93
5.6 Concluding Remarks 95
Acknowledgements 95
References 96
6 Reconstitution of General Diffusion Pores from Bacterial Outer Membranes 99
Christophe Danelon and Mathias Winterhalter
6.1 Introduction 99
6.2 Planar Lipid Bilayer Technique 100
6.3 Intrinsic Properties of General Diffusion Channels 101
6.3.1 Single channel Analysis of OmpF Gating 101
6.3.2 Molecular Origin of Voltage Gating 103
6.3.3 Effect of Membrane Composition and OmpF LPS Interactions 105
6.3.4 Open channel Conductance 106
6.3.5 Voltage Effect and Channel Orientation 108
6.3.6 Ion Selectivity 108
6.3.7 The Permeating Cations Interact with Specific Elements
along the Ionic Pathway 110
6.3.8 Single channel Recordings versus Free Energy Calculation 111
6.4 OmpF as a Specific Channel: Antibiotic Translocation 111
6.5 Application: Nanoreactor 114
Acknowledgments 115
References 115
VIII I Contents
7 OmpA/OprF: Slow Porins or Channels Produced by
Alternative Folding of Outer Membrane Proteins 119
Etsuko Sugawara and Hiroshi Nikaido
7.1 Introduction 119
7.2 Controversies on OprF as the Major Porin of P. aeruginosa 121
7.2.1 Is the OprF Channel Wider than those of E. coli Porins? 121
7.2.2 Is OprF a Porin? 124
7.2.3 Is OprF the Major Porin in P. aeruginosa? 124
7.3 Similarity between OmpA and OprF 127
7.3.1 OmpA is also an Inefficient Porin 127
7.3.2 The Majority of OmpA and OprF Folds into Two domain Conformers 128
7.4 The Minority, Open channel Conformers of OmpA/OprF 129
7.5 The Nature of the Open Conformer 131
7.6 Regulation of Expression of ompA and oprF Genes 134
References 136
8 Drug Efflux and Protein Export through Channel tunnels 139
Christian Andersen
8.1 Introduction 139
8.2 Channel tunnel dependent Export Systems 140
8.2.1 The Type I Secretion System 140
8.2.1.1 Substrates 141
8.2.1.2 The Inner Membrane Transporters of Type I Secretion Systems 142
8.2.1.3 The Accessory Protein of the Type I Secretion System 143
8.2.2 Efflux Pumps 144
8.2.2.1 The Inner Membrane Transporters of Channel tunnel dependent
Efflux Pumps 144
8.2.2.2 Accessory Proteins of Multidrug Efflux Pumps 148
8.2.3 Comparison of Channel tunnel dependent Export Systems
in E. coli and P. aeruginosa 149
8.3 Channel Tunnels 151
8.3.1 The Structure of TolC 152
8.3.1.1 The Channel Domain 153
8.3.1.2 The Tunnel Domain 154
8.3.2 Electrophysiological Characterization of TolC 155
8.3.2.1 The Role of Aspartate Residues at the Periplasmic Entrance 156
8.3.2.2 Opening of the Periplasmic Entrance 156
8.4 Model for TolC dependent Export 157
8.4.1 The Role of the Accessory Protein 258
8.4.2 The Mechanism of Protein Secretion 158
8.4.3 The Mechanism of Efflux Pumps 160
8.4.3.1 Substrate Binding of Different Transporters 360
8.4.3.2 Export of Substances by RND Transporters Exclusively
from the Periplasm? 161
Contents I IX
8.5 Conclusion 162
Acknowledgments 163
References 163
9 Structure Function Relationships in Sugar specific Porins 169
Tilman Schirmer
9.1 Introduction 269
9.2 Maltoporin and Sucrose Porin 171
9.3 Probing Function by Site directed Mutagenesis 172
9.3.1 Ionic Tracks 172
9.3.2 Greasy Slide 172
9.3.3 Tyrosine 118 173
9.3.4 Translocation Kinetics 174
9.3.5 Changing Substrate Specificity 175
9.3.6 N terminal Domain of ScrY 177
9.3.7 Probing the Role of the External Loops 177
9.4 Simulation of Maltoporin Function 178
References 180
10 Functional Reconstitution of Specific Porins 183
Roland Benz and Frank Orlik
10.1 Introduction 183
10.2 Isolation and Purification of Specific Porins 185
10.3 Reconstitution of Specific Porins in Lipid Bilayer Membranes 186
10.4 Analysis of Substrate Transport through Specific Porin Channels 188
10.4.1 Study of Ion Transport through the Phosphate specific OprP
of P. aeruginosa 188
10.4.2 Evaluation of the Stability Constant for Binding of Neutral Solutes
to the Binding Site inside Specific Porins 193
10.4.3 Investigation of Substrate binding Kinetics using the Analysis
of Current Fluctuations 195
10.5 Study of Carbohydrate Binding to the Specific Porins
of the LamB Family 196
10.5.1 LamB of E. coli 196
10.5.1.1 Study of LamB (Maltoporin) Mutants 200
10.5.2 ScrY (Sucrose Porin) of Enteric Bacteria 202
10.5.2.1 Study of Carbohydrate Binding to ScrY Mutants 204
10.6 Properties of the Cyclodextrin (CD) specific Outer Membrane
Porin CymA of Klebsiella oxytoca 205
10.7 Porin 0mpP2 of Haemophilus infiuenzae is a Specific Porin for
Nicotinamide derived Nucleotide Substrates 206
10.8 Study of the Nudeoside specific Tsx of E. coli 207
10.9 Conclusions 209
Acknowledgements 210
References 210
X I Contents
11 Energy coupled Outer Membrane Iron Transporters ^23
Volkmar Braun and Michael Braun
11.1 Common Features of Outer Membrane iron Transporters 213
11.1.1 Energy Coupling of Transport 214
11.1.2 Iron Sources 215
11.1.3 Regulation 216
11.1.4 Transport across the Cytoplasmic Mernbrane 217
11.2 Crystal Structures of Energy coupled Outer Membrane
Transport Proteins 217
11.2.1 FhuA Transporter and Receptor 219
11.2.1.1 The Transport Activity of FhuA 219
11.2.1.2 Substrate Specificity of the E. coli FhuA Transporter 221
11.2.1.3 The Receptor Activity of FhuA 222
11.2.1.4 Analysis of Previously Isolated Mutants in the Light
of the FhuA Crystal Structure 224
11.2.2 FecA Transporter and Signaler 225
11.2.2.1 Transport Activity of FecA 225
11.2.2.2 Signaler Function of FecA 226
11.2.3 FepA Transporter and Receptor 227
11.2.3.1 FepA Transport Activity for Fe3+ Enterobactin
and Receptor Activity for Colicin B 227
11.3 Other Fe3+ Siderophore Transporters 22s
11.3.1 The Outer Membrane Protein FpvA off. flcruginosa Transports
Fe3+ Pyoverdin 228
11.3.2 The IroN Protein Transports Salmocheh'n 229
11.3.3 FyuA Transports Fe3+Yersiniabactin 229
11.4 Outer Membrane Proteins that Transport Heme 229
11.5 Outer Membrane Proteins that Transport Ir°n Delivered
as Transferrin and Lactoferrin 231
11.6 Perspectives 232
Acknowledgments 233
References 233
12 Structural and Functional Aspects of the Vitarnin B12 Receptor BtuB 237
Robert J. Kadner, David P. Chimento and Nathalie Cadieux
12.1 Introduction 237
12.1.1 Overview 237
12.1.2 Cbl Uptake and Utilization 238
12.1.3 Transport Components 239
12.1.4 Colicins and Phages 241
12.2 BtuB Structure 242
12.2.1 Shared Structural Features 242
12.2.1.1 The p barrel 243
\1.1.\2 Periplasmic Turns 244
12.2.1.3 External Loops 244
Contents I XI
12.2.1.4 The Hatch Domain 245
12.2.2 Calcium Binding 245
12.2.3 Cbl binding Surfaces 247
12.2.3.1 Comparison to Iron Siderophore Binding Surfaces 248
12.2.4 The Ton Box 248
12.2.4.1 Interaction of the Ton Box and TonB 248
12.3 BtuB Dynamics 250
12.3.1 Site directed Spin Labeling 250
12.3.2 Substrate induced Changes in the Ton Box 251
12.3.3 Transmembrane Region and Barrel Dynamics 252
12.3.4 Comparison to Crystal Structure 253
12.4 Revisiting Old Data 253
12.4.1 phoA Fusions 253
12.4.2 Behavior of In frame Deletions 254
12.5 Myths and Models about TonB dependent Transport Mechanism 255
12.5.1 "Ligand gated Pores"? 255
12.5.2 The Barrel is Sufficient for TonB dependent Transport? 255
12.5.3 The Hatch Stays in the Barrel? 256
12.6 Conclusions 256
Acknowledgments 257
References 257
13 Structure and Function of Mitochondrial (Eukaryotic) Porins 259
Roland Benz
13.1 Introduction 259
13.2 Reconstitution of Mitochondrial Porins (VDACs) in
Model Membranes 261
13.2.1 Isolation and Purification of Mitochondrial Porins 261
13.2.2 Heterologous Expression of Eukaryotic Porins 261
13.2.3 Reconstitution Methods 262
13.3 Characterization of the Pore forming Properties of Eukaryotic Porins 264
13.3.1 Single channel Analysis of the Mitochondrial Pore in the Open State 264
13.3.2 Eukaryotic Porins are Voltage Gated 265
13.3.3 Single channel Conductance of the Closed States 270
13.3.4 Selectivity of the Open and Closed State of Eukaryotic Porins 270
13.4 Inhibition of the Mitochondrial Pore 272
13.5 Structure of the Channel formed by Eukaryotic Porins 274
13.5.1 Primary Structure of Eukaryotic Porins 274
13.5.2 Secondary Structure of Mitochondrial Porins 276
13.5.3 Structure of the Channel forming Unit 277
13.5.4 Are Sterols Involved in the Formation of the Channel forming Unit? 280
13.5.5 Electron Microscopic Analysis of N. crassa Porin 280
13.6 Conclusions 281
Acknowledgments 282
References 282
XII Contents
14 Mitochondrial Porins in Mammals:
Insights into Functional Roles from Mutant Mice and Cells 285
Keltoum Anjlous and William J. Craigen
14.1 Introduction 285
14.2 Channel Activity 286
14.3 The Genetics of Mammalian VDACs 287
14.4 Generation of mammalian VDAC mutants 289
14.5 The Role of the Mitochondrial Outer Membrane
in Compartmentalization of High energy Metabolites 290
14.6 VDAC Cytoskeletal Interactions 294
14.7 Mitochondrial Permeability Transition Pore and Synaptic Functions 296
14.8 VDACs and Apoptosis 301
14.9 Conclusions 302
Acknowledgments 303
References 303
15 Cene Family Expression and Multitopological Localization
of Eukaryotic Porin/Voltage Dependent Anion selective Channel (VDAC):
Intracellular Trafficking and Alternative Splicing 309
Vito De Pinto and Angela Messina
15.1 Introduction 309
15.2 Molecular Biology of Porin/VDAC Gene Families 309
15.2.1 Porin/VDAC in the Yeast S. cerevisiae 310
15.2.2 Porin/VDAC Gene Family in D. melanogaster.
Evidence of Alternative Splice Variants 311
15.2.3 Porin/VDAC Gene Families in Mammals 314
15.2.3.1 Porin/VDAC Gene Family in Mouse:
More Evidence of Alternative Splicing 314
15.2.3.2 Porin/VDAC Genes in Human 316
15.2.4 Porin/VDAC Gene Families in Plants 320
15.2.5 Molecular Evolution of Porin/VDAC 321
15.3 Multitopological Localization of VDAC in the Cell 323
15.3.1 Porin in the Plasma Membrane 323
15.3.1.1 Targeting to the Plasma Membrane 325
15.3.1.2 Porin/VDAC Function in the Plasma Membrane 327
15.3.2 Interactions of Porin with other Cellular Structures 328
15.4 Conclusions 330
Acknowledgments 330
References 330
Contents I XIII
16 Function of the Outer Mitochondrial Membrane Pore
(Voltage dependent Anion Channel) in Intracellular Signaling 339
Mikhail Vyssokikh and Dieter Brdiczka
16.1 Introduction 339
16.2 Structure and Isotypes of VDAC 339
16.3 The Influence of Phospholipids on VDAC Structure 340
16.4 VDAC Conductance and Ion Selectivity 341
16.5 Physiological Significance of the Voltage Dependence 341
16.6 Porins as Specific Binding Sites 342
16.7 VDAC senses Inner Membrane Functions in the Contact Site 342
16.8 Cytochrome c is a Component of the Contact Sites 345
16.9 Isolation and Characterization of VDAC ANT Complexes 345
16.10 Reconstitution of VDAC ANT Complexes 345
16.11 Importance of Metabolic Channeling in Regulation of
Energy Metabolism 346
16.12 The VDAC ANT Complex as Permeability Transition Pore 347
16.13 The VDAC ANT Complex as a Target for Bax dependent
Cytochrome c Release 349
16.14 The VDAC ANT Complexes contain Cytochrome c 351
16.15 The Importance of the Kinases in Regulation of Apoptosis 352
16.16 Suppression of Bax dependent Cytochrome c Release
and Permeability Transition by Hexokinase 352
16.17 Suppression of Permeability Transition by Mitochondrial
Creatine Kinase 353
16.18 Conclusion 353
References 354
Index 349 |
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| bvnumber | BV019360422 |
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| callnumber-raw | QP551 |
| callnumber-search | QP551 |
| callnumber-sort | QP 3551 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WE 5000 WE 5160 |
| classification_tum | CHE 887f BIO 470f BIO 200f BIO 770f BIO 220f CHE 820f |
| ctrlnum | (OCoLC)56515197 (DE-599)BVBBV019360422 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| format | Book |
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| id | DE-604.BV019360422 |
| illustrated | Illustrated |
| indexdate | 2025-02-04T09:00:49Z |
| institution | BVB |
| isbn | 3527307753 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-012824169 |
| oclc_num | 56515197 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-703 DE-11 |
| owner_facet | DE-M49 DE-BY-TUM DE-703 DE-11 |
| physical | XX, 366 S. Ill., graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Bacterial and eukaryotic porins structure, function, mechanism ed. by Roland Benz Weinheim Wiley-VCH 2004 XX, 366 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bacteriologia médica larpcal Biologia celular larpcal Molécules Proteínas (estrutura) larpcal Protéines - Structure Eukaryotic Cells physiology Molecules Porins physiology Proteins Structure Porin (DE-588)4221584-5 gnd rswk-swf Porin (DE-588)4221584-5 s DE-604 Benz, Roland 1943-2025 Sonstige (DE-588)129268933 oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012824169&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bacterial and eukaryotic porins structure, function, mechanism Bacteriologia médica larpcal Biologia celular larpcal Molécules Proteínas (estrutura) larpcal Protéines - Structure Eukaryotic Cells physiology Molecules Porins physiology Proteins Structure Porin (DE-588)4221584-5 gnd |
| subject_GND | (DE-588)4221584-5 |
| title | Bacterial and eukaryotic porins structure, function, mechanism |
| title_auth | Bacterial and eukaryotic porins structure, function, mechanism |
| title_exact_search | Bacterial and eukaryotic porins structure, function, mechanism |
| title_full | Bacterial and eukaryotic porins structure, function, mechanism ed. by Roland Benz |
| title_fullStr | Bacterial and eukaryotic porins structure, function, mechanism ed. by Roland Benz |
| title_full_unstemmed | Bacterial and eukaryotic porins structure, function, mechanism ed. by Roland Benz |
| title_short | Bacterial and eukaryotic porins |
| title_sort | bacterial and eukaryotic porins structure function mechanism |
| title_sub | structure, function, mechanism |
| topic | Bacteriologia médica larpcal Biologia celular larpcal Molécules Proteínas (estrutura) larpcal Protéines - Structure Eukaryotic Cells physiology Molecules Porins physiology Proteins Structure Porin (DE-588)4221584-5 gnd |
| topic_facet | Bacteriologia médica Biologia celular Molécules Proteínas (estrutura) Protéines - Structure Eukaryotic Cells physiology Molecules Porins physiology Proteins Structure Porin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012824169&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT benzroland bacterialandeukaryoticporinsstructurefunctionmechanism |