Biosensors: a practical approach
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Oxford Univ. Press
2004
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | A practical approach series
268 |
| Schlagworte: | |
| Online-Zugang: | Publisher description Table of contents only Inhaltsverzeichnis |
| Beschreibung: | Hier auch später erschienene, unveränderte Nachdrucke |
| Beschreibung: | XVI, 251 S. Ill., graph.Darst. |
| ISBN: | 0199638462 0199638454 9780199638451 |
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Datensatz im Suchindex
| _version_ | 1804132738107703296 |
|---|---|
| adam_text | Titel: Biosensors
Autor: Cooper, Jonathan M
Jahr: 2004
Contents
Protocol list xi
Abbreviations xiii
Contributors xv
Redox hydrogel-based electrochemical biosensors 1
Mam Heller
1 Electron conducting redox polymers in biosensors 1
1.1 Relationship to mediator-based sensors 1
1.2 The electrocatalytic activity of redox hydrogels and
the wiring of enzymes 2
1.3 Dependence of the diffusivity of electrons on
crosslinking and the advantage of composite electrodes 2
1.4 The redox polymers and their electrochemistry 3
1.5 Crosslinkers and crosslinking 4
2 Enzyme electrodes 4
2.1 Electron transfer between enzyme and polymer
redox centers 4
2.2 Microscopic homogeneity and salt effects in the
redox polymer-enzyme system 6
2.3 Optimal compositions 6
2.4 Special matrices 7
3 Specific sensor examples 7
3.1 Amperometric and potentiometric biosensors of substrates of
wired redox enzymes 7
3.2 Sensor measuring the turnover rate of hydrolytic and other
non-redox enzymes 8
3.3 In vivo glucose sensors 10
3.4 Affinity sensors 10
Hybridization at oligonucleotide sensitive electrodes 19
Daren J. Caruana
1 Introduction 19
2 Function of oligonucleotide sensitive electrodes 20
3 Hybridization efficiency and sensitivity 21
CONTENTS
4 Probe oligonucleotide structure and dynamics 22
4.1 Surface concentration 22
4.2 Probe length and orientation 23
4.3 Attachment of probe 25
5 Hybridization conditions 30
5.1 Temperature 31
5.2 Ionic strength 32
5.3 Base mismatch 33
5.4 Mass transport 34
5.5 Nonspecific adsorption 34
5.6 Other factors 36
6 Hybridization kinetics 36
7. Summary 38
Screen-printing methods for biosensor production 41
Xian-En Zhang
1 Introduction 41
2 Screen-printing technology 42
2.1 Materials and methods 42
2.2 Apparatus 47
2.3 Printing patterns 48
2.4 Printing process 49
3 Applications 51
3.1 Clinical diagnosis 51
3.2 Food analysis bioprocess control 52
3.3 Environmental monitoring 52
3.4 Other approaches 54
4 Conclusion 55
Kinetic modeling for biosensors 59
Philip Bartlett and Chee-Seng Toh
1 Introduction 59
1.1 The purpose and practice of modeling 59
1.2 Enzyme kinetics 60
1.3 Basic electrochemistry 63
2 Modeling 69
2.1 The flux diagram for the membrane|enzyme|electrode 70
2.2 Simplifying assumptions 70
2.3 The flux equations 71
2.4 Solution of flux equations 73
2.5 Deriving a complete kinetic model 78
2.6 Experimental verification of approximate analytical kinetic models 81
2.7 Numerical simulation methods 82
3 Applications 89
4 Kinetic modeling in other types of biosensors 89
4.1 Potentiometric enzyme electrodes 90
4.2 Optical and photometric biosensors 90
4.3 Immunosensors 91
vin
CONTENTS
5 Conclusions 92
List of symbols 92
Bio-, chemi-, and electrochemiluminescence for
fiber-optic biosensors 97
Loi c J. Blum and Pierre R. Coulet
1 Introduction 97
2 Design of the biosensor 97
2.1 Optical waveguide 97
2.2 Setup 98
2.3 Light-emitting reactions 100
2.4 Preparation of the sensing layer 101
3 Examples of determinations with the luminescence sensors 105
3.1 ATP determination 105
3.2 NADH determination 305
3.3 Extension to other analytes using dehydrogenases as
auxiliary enzymes 105
3.4 H202 determination 106
3.5 Extension to other analytes involving H202 detection 107
4 Concluding remarks 108
Determination of metal ions by fluorescence aniostropy:
A practical biosensing approach 109
Richard Thompson, Badri Maliwal, Hui Hui Zeng, and Michele Loetz Cramer
1 Introduction and rationale 109
1.1 Why fluorescence anisotropy to determine metal ions? 109
2 Theory of anisotropy-based determination of metal ions 111
2.1 Reagent approaches 111
2.2 Reagentless approach 112
3 Fluorescent aryl sulfonamides for zinc(II) determination 114
4 Removal of zinc from carbonic anhydrase (CA) 115
5 Avoidance of metal ion contamination 117
6 Determination of Zn using a reagent approach 119
7 Determination of Cu and other ions by using a
reagentless approach 123
8 Calibration of anisotropy 124
Fluorescence-based fiber-optic biosensors 131
David R. Walt, Caroline L. Schauer, Shannon E. Stitzel, Michael S. Fleming,
and Jason R. Epstein
1 Introduction 131
1.1 Fiber polishing 133
2 Single-analyte detection using an enzymatic sensing layer 134
2.1 Enzymatic sensing layer 134
2.2 PAN gel immobilization 135
rx
CONTENTS
3 Multi-analyte arrays 136
3.1 Immobilization via polymer photodeposition 136
3.2 Microwell array platform preparation 138
3.3 Live-cell array fabrication 146
4 Conclusions 151
Functional analysis of ion channels: Planar patch clamp
and impedance spectroscopy of tethered lipid membranes 153
Michael Mayer, Samuel Terrettaz, Laurent Giovangrandi,
Thierry Stora, and Horst Vogel
1 Introduction 153
2 Planar patch clamp 154
2.1 Concept of patch clamp on a chip 154
2.2 Formation of planar bilayers on a chip 157
2.3 Chip-based planar bilayers: single-channel measurements of
alamethicin pores 165
3 Impedance spectroscopy of tethered lipid membranes 168
3.1 Basics of impedance spectroscopy 168
3.2 Measuring technique and electrochemical cell 170
3.3 Hybrid lipid layer 170
3.4 Tethered lipid bilayers 174
3.5 Lipid bilayer tethered via surface-attached proteins 177
3.6 Highly insulating tethered lipid bilayers for
single-channel experiments 379
Protein engineering for biosensors 185
Gianfranco Gilardi
1 Introduction 185
2 Rational protein engineering 187
2.1 Modeling and calculations on protein structures 388
2.2 Site-directed mutagenesis 399
3 Directed evolution 222
3.1 Random mutagenesis: error prone PCR 224
3.2 Recombination: DNA shuffling 226
3.3 Functional screening of the library 230
4 Functional characterization of the mutants 234
5 Other aspects of protein engineering 234
6 Concluding remarks 238
Index 241
|
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| spelling | Biosensors a practical approach ed. by Jonathan M. Cooper ... 2. ed. Oxford [u.a.] Oxford Univ. Press 2004 XVI, 251 S. Ill., graph.Darst. txt rdacontent n rdamedia nc rdacarrier A practical approach series 268 Hier auch später erschienene, unveränderte Nachdrucke Biosenzorji ssg Mikrobiologija ssg Biosensors Biosensor (DE-588)4193016-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Biosensor (DE-588)4193016-2 s DE-604 Cooper, Jonathan M. edt A practical approach series 268 (DE-604)BV007921321 268 text/html http://www.loc.gov/catdir/enhancements/fy0617/2004046144-d.html Publisher description text/html http://www.loc.gov/catdir/enhancements/fy0617/2004046144-t.html Table of contents only HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012787942&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Biosensors a practical approach A practical approach series Biosenzorji ssg Mikrobiologija ssg Biosensors Biosensor (DE-588)4193016-2 gnd |
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| title | Biosensors a practical approach |
| title_auth | Biosensors a practical approach |
| title_exact_search | Biosensors a practical approach |
| title_full | Biosensors a practical approach ed. by Jonathan M. Cooper ... |
| title_fullStr | Biosensors a practical approach ed. by Jonathan M. Cooper ... |
| title_full_unstemmed | Biosensors a practical approach ed. by Jonathan M. Cooper ... |
| title_short | Biosensors |
| title_sort | biosensors a practical approach |
| title_sub | a practical approach |
| topic | Biosenzorji ssg Mikrobiologija ssg Biosensors Biosensor (DE-588)4193016-2 gnd |
| topic_facet | Biosenzorji Mikrobiologija Biosensors Biosensor Aufsatzsammlung |
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