Investigations on the mechanism of action of antiinflammatory glucan sulfates:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 117 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1804130559196135424 |
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| adam_text | INVESTIGATIONS ON THE MECHANISM OF ACTION OF ANTIINFIAMMATORY GIUCAN
SUIFATES DISSERTATION ZUR ERIANGUNG DES DOKTORGRADES DER
NATURWISSENSCHAFTEN (DR. RER. NAT.) DER NATURWISSENSCHAFTLICHEN FAKULTAT
IV - CHEMIE UND PHARMAZIE - DER UNIVERSITAT REGENSBURG VORGELEGT VON
MARIA LAFFRANCHLNI AUS MONFALCONE (ITALIEN) 2004 INVESTIGATIONS ON THE
MECHANISM OF ACTION OF ANTIINFIAMMATORY GIUCAN SUIFATES DISSERTATION ZUR
ERIANGUNG DES DOKTORGRADES DER NATURWISSENSCHAFTEN (DR. RER. NAT.) DER
NATURWISSENSCHAFTLICHEN FAKULTAT IV - CHEMIE UND PHARMAZIE - DER
UNIVERSITAT REGENSBURG VORGELEGT VON MARIA LAFFRANCHLNI AUS MONFALCONE
(ITALIEN) 2004 INVESTIGATIONS ON THE MECHANISM OF ACTION OF
ANTIINFIAMMATORY GIUCAN SUIFATES DISSERTATION ZUR ERIANGUNG DES
DOKTORGRADES DER NATURWISSENSCHAFTEN (DR. RER. NAT.) DER
NATURWISSENSCHAFTLICHEN FAKULTAT IV - CHEMIE UND PHARMAZIE - DER
UNIVERSITAT REGENSBURG VORGELEGT VON MARIA LAFFRANCHLNL AUS MONFALCONE
(ITALIEN) 2004 I A. INTRODUCTION 1 1. THE IMMUNE SYSTEM 1 2. CELLULAR
NON SPECIFIC IMMUNE SYSTEM 2 2.1. LEUCOCYTES 2 2.2. POLYMORPHNUCLEAR
GRANULOCYTES (PMN) 2 2.3. MONOCYTES 3 3. LEUCOCVTE MIERATION 3 3.1.
TYPES OF CELL MOVEMENT 3 3.2. CHEMOATTRACTANTS 4 3.3. CELL ADHESION 5 4.
CYTOKINES 6 4.1. INTERLEUKIN-1 (IL-1) 7 4.2. TUMOUR NECROSIS-FACTOR
ALPHA (TNF-A) 7 4.3. INTERLEUKIN-6 (IL-6) 8 4.4. THE CHEMOKINE
SUPERFAMILY 8 4.4.1. INTERLEUKIN-8 (IL-8) 9 4.5. INTERLEUKIN 10 (IL-10)
9 5. COMPLEMENT SYSTEM 10 5.1. COMPOSITION OF THE COMPLEMENT SYSTEM 10
5.1.1. THE CLASSICAL PATHWAY OF COMPLEMENT ACTIVATION 10 5.1.2. THE
ALTERNATIVE PATHWAY OF COMPLEMENT ACTIVATION 11 5.1.3. THE MEMBRANE
ATTACK COMPLEX (MAC)^ 12 5.1.4. ATYPICAL COMPLEMENT ACTIVATION 12 5.2.
REGULATORS OF COMPLEMENT ACTIVATION (RCA) 13 5.3. DISEASES RELATED WITH
DEFICIENCY OF THE COMPLEMENT REGULATION 14 6. IMMUNE SYSTEM AND DISEASES
15 7. ACTUAL THERAPIES OF INFLAMMATORY DISORDERS 15 7.1. NONSTEROIDAL
ANTI-INFLAMMATORY DRUGS (NSAIDS) 17 7.2. GLUCOCORTICOIDS 17 7.3. OTHER
ANTI-INFLAMMATORY COMPOUNDS 18 7.4. SULFATED POLYSACCHARIDES AS
POTENTIAL ANTIINFIAMMATORY DRUGS 19 7.4.1. EFFECTS OF HEPARIN 19 7.4.2.
SULFATED POLYSACCHARIDES AS ALTERNATIVE OF HEPARIN 20 B. AIM OF THE
STUDY 21 II 1. INVESTIGATED COMOOUNDS .I 21 2. CHOICE OF THE
PHYSIOLOGICAL TESTS 21 3. PHYSIOLOGICAL TESTS 22 C. PREPARATION AND
CHARACTERISATION OF SEMISYNTHETIC SULFATED POLYSACCHARIDES 23 1. CHOICE
OF THE STARTING COMPOUNDS 23 L.L.CURDLAN 23 1.2. LAMINARIN 24
1.3.PULLULAN 24 2. PREPARATION OF SEMISYNTHETIC SULFATED POLYSACCHARIDES
25 2.1. SULFATION OF THE COMPOUNDS 25 2.2. FRACTIONATION OF SULFATED
POLYSACCHARIDES 25 D. PHYSIOLOGICAL TESTING 30 1. COMPLEMENT ACTIVATION
30 1.1. PULLULAN SUIFATES (PULS) AS COMPLEMENT ACTIVATION INHIBITORS 30
1.1.1. INFLUENCE OF PULLULAN SUIFATES ON THE CLASSICAL COMPLEMENT
ACTIVATION 30 1.1.2. INFLUENCE OF PULLULAN SUIFATES ON THE ALTERNATIVE
COMPLEMENT ACTIVATION 35 1.2. CURDLAN SUIFATES (CURS) AS COMPLEMENT
ACTIVATION INHIBITORS 36 1.2.1. INFLUENCE OF CURDLAN SUIFATES ON THE
CLASSICAL COMPLEMENT ACTIVATION 36 1.2.2. INFLUENCE OF CURDLAN SUIFATES
ON THE ALTERNATIVE COMPLEMENT ACTIVATION 38 1.3. DS INFLUENCE ON THE
ACTIVITY OF PULLULAN SUIFATES 38 1.4. COMPARISON BETWEEN PULS AND CURS
ANTICOMPLEMENTARY ACTIVITY 39 1.5. APPENDIX 41 1.5.1. STRUCTURAL
PARAMETERS AND ICJO VALUES OF PULS FRACTIONS 41 1.5.2. STRUCTURAL
PARAMETERS AND ICSO VALUES OF CURS FRACTIONS 42 2. LEUKOCVTE CHEMOTAXIS
44 2. L.IN-VITRO INVESTIGATIONS OF LEUCOCYTE CHEMOTAXIS 44 2.1.1. THE
BOYDEN-CHAMBER 44 2.2. CELLS FOR CHEMOTAXIS EXPERIMENTS 46 2.2.1. PMN
AND MONOCYTE ISOLATIONS 46 2.2.2. INVESTIGATIONS ON HUMAN MONOCYTIC CELL
LINE MONOMAC-6 CHEMOTAXIS 47 2.3. CHEMOTACTIC AGENTS 47 M 2.3.1.
ZYMOSAN-ACTIVATED-SERUM, COMPLEMENT FRAGMENT C5A AND INTERLEUKIN-8 FOR
PMN-CHEMOTAXIS 47 2.3.2. MCP-1 AND ZAS FOR MONOCYTE CHEMOTAXIS 49 2.4.
CHOICE OF THE MEMBRANE 50 2.5. INFLUENCE OF GIUCAN SUIFATES ON
CHEMOTAXIS 51 2.5.1. EFFECTS OF PS3 AND UFH ON PMN CHEMOTAXIS 51 2.5.2.
STRUCTURE-ACTIVITY RELATIONSHIPS 53 2.5.2.1. DEPENDENCY OF PMN
CHEMOTAXIS INHIBITORY ACTIVITY ON THE DS 53 2.5.2.2. INFLUENCE OF THE
MOLECULAR WEIGHT ON PMN CHEMOTAXIS 55 2.5.2.3. DEPENDENCY OF THE PMN
CHEMOTAXIS INHIBITORY ACTIVITY ON THE BASIC STRUCTURE 57 2.5.3. EFFECT
OF PS3 AND UFH ON MONOCYTE CHEMOTAXIS 58 2.5.4. STRUCTURE-ACTIVITY
RELATIONSHIPS 59 2.5.4.1. INFLUENCE OF THE DEGREE OF SULFATION 59
2.5.4.2. INFLUENCE OF THE MOLECULAR WEIGHT 60 2.5.4.3. INFLUENCE OF THE
BASIC STRUCTURE 61 2.5.5. COMPARISON BETWEEN PMN AND MONOCYTE CHEMOTAXIS
61 2.5.6. INVESTIGATIONS ON THE MODE OF ACTION 63 2.5.6.1. PS3-CELLS
INTERACTIONS 63 2.5.6.2. PS3-CHEMOATTRACTANT INTERACTIONS 64 2.5.6.3.
INFLUENCE OF PS3 ON THE CHEMOKINESIS 65 2.6. SUMMARY AND DISCUSSION . .
67 3. INVESTIGATION OF CYTOKINE RELEASE 68 3.1. EVALUATION OF PARAMETERS
INFLUENCING THE CYTOKINE RELEASE 68 3.1.1. TIME-DEPENDENT CYTOKINE
RELEASE BY MONOCYTES 68 3.1.2. INDUCTION OF CYTOKINE RELEASE BY U937 71
3.1.3. INDUCTION OF CYTOKINE RELEASE BY MONOMAC-6 73 3.2. INFLUENCE OF
GIUCAN SUIFATES ON THE CYTOKINES RELEASE 74 3.2.1. CYTOKINE RELEASE BY
LPS-ACTIVATED MONOCYTES UND MONOMAC-6 74 3.2.2. CYTOKINE RELEASE BY
LPS-ACTIVATED MONOCYTES CULTIVATED IN THE PRESENCE OF 2% SERUM 78 3.2.3.
SPONTANEOUS RELEASE OF CYTOKINES BY NON-STIMULATED MONOCYTES 83 3.3.
SUMMARY 84 E. SUMMARY 85 IV 1. ANTIINFLAMMATORY EFFECTS OF GIUCAN
SUIFATES 85 2. PREPARATION OF GIUCAN SUIFATES
***************.*.*****..**.***.. * 85 3. INFLUENCE ON THE COMPLEMENT
ACTIVATION 85 4. INFLUENCE ON THE LEUCOCYTE CHEMOTAXIS 85 5. INFLUENCE
ON THE CYTOKINE RELEASE 86 F. MATERIALS AND METHODS 87 1. GENERAL
INFORMATION 87 1.1. LABORATORY EQUIPMENT 87 1.2. LABORATORY MATERIALS 88
2. TEST COMPOUNDS 88 2.1. COMMERCIALLY AVAILABLE SULFATED
POLYSACCHARIDES 88 2.2. SEMISYNTHETIC GIUCAN SUIFATES 89 2.3. SYNTHESIS
OF PULS AND CURS 89 3. FRACTIONATION OF PULS AND CURS.....; 90 4.
ANALYTIC METHODS 91 4.1. DETERMINATION OF THE DS AND MW 91 5. COMPLEMENT
MODULATION ASSAY (CMA) 91 5.1. CLASSIC COMPLEMENT ACTIVATION
INVESTIGATION (CCMA) 92 5.2. ALTERNATIVE COMPLEMENT ACTIVATION
INVESTIGATION (ACMA) 92 6. LEUCOCYTE CHEMOTAXIS ....93 6.1. LEUCOCYTE
ISOLATION 93 6.2. MONOMAC-6 CULTURE 95 6.3. CHEMOTAXIS ASSAY 96 7.
CYTOKINE RELEASE ASSAY 98 7.1. CELLS 99 7.2. CYTOKINE MEASUREMENT 100
7.3. MTT ASSAY 100 8. ABBREVIATIONS 102 G. BIBLIOGRAFY 103
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| author | Laffranchini, Maria |
| author_facet | Laffranchini, Maria |
| author_role | aut |
| author_sort | Laffranchini, Maria |
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| bvnumber | BV017894915 |
| ctrlnum | (OCoLC)249259534 (DE-599)BVBBV017894915 |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T19:22:57Z |
| institution | BVB |
| language | English |
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| physical | IV, 117 S. Ill., graph. Darst. |
| publishDate | 2004 |
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| spelling | Laffranchini, Maria Verfasser aut Investigations on the mechanism of action of antiinflammatory glucan sulfates vorgelegt von Maria Laffranchini 2004 IV, 117 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2004 Antiphlogistikum (DE-588)4123144-2 gnd rswk-swf Sulfate (DE-588)4138384-9 gnd rswk-swf Wirkungsmechanismus (DE-588)4292866-7 gnd rswk-swf Glucane (DE-588)4157662-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Glucane (DE-588)4157662-7 s Sulfate (DE-588)4138384-9 s Antiphlogistikum (DE-588)4123144-2 s Wirkungsmechanismus (DE-588)4292866-7 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010732854&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Laffranchini, Maria Investigations on the mechanism of action of antiinflammatory glucan sulfates Antiphlogistikum (DE-588)4123144-2 gnd Sulfate (DE-588)4138384-9 gnd Wirkungsmechanismus (DE-588)4292866-7 gnd Glucane (DE-588)4157662-7 gnd |
| subject_GND | (DE-588)4123144-2 (DE-588)4138384-9 (DE-588)4292866-7 (DE-588)4157662-7 (DE-588)4113937-9 |
| title | Investigations on the mechanism of action of antiinflammatory glucan sulfates |
| title_auth | Investigations on the mechanism of action of antiinflammatory glucan sulfates |
| title_exact_search | Investigations on the mechanism of action of antiinflammatory glucan sulfates |
| title_full | Investigations on the mechanism of action of antiinflammatory glucan sulfates vorgelegt von Maria Laffranchini |
| title_fullStr | Investigations on the mechanism of action of antiinflammatory glucan sulfates vorgelegt von Maria Laffranchini |
| title_full_unstemmed | Investigations on the mechanism of action of antiinflammatory glucan sulfates vorgelegt von Maria Laffranchini |
| title_short | Investigations on the mechanism of action of antiinflammatory glucan sulfates |
| title_sort | investigations on the mechanism of action of antiinflammatory glucan sulfates |
| topic | Antiphlogistikum (DE-588)4123144-2 gnd Sulfate (DE-588)4138384-9 gnd Wirkungsmechanismus (DE-588)4292866-7 gnd Glucane (DE-588)4157662-7 gnd |
| topic_facet | Antiphlogistikum Sulfate Wirkungsmechanismus Glucane Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010732854&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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