Protein purification protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
244 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 484 S. Ill., graph. Darst. |
| ISBN: | 159259655X 1588290670 |
Internformat
MARC
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| 245 | 1 | 0 | |a Protein purification protocols |c ed. by Paul Cutler |
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Datensatz im Suchindex
| _version_ | 1804130552682381312 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
1 General Strategies
Shawn Doonan and Paul Cutler 1
2 Preparation of Extracts From Animal Tissues
/. Mark Skehel 15
3 Protein Extraction From Plant Tissues
Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry 21
4 Extraction of Recombinant Protein From Bacteria
Anne F. McGettrick and D. Margaret Worrall 29
5 Protein Extraction From Fungi
Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds 37
6 Subcellular Fractionation of Animal Tissues
Norma M. Ryan 47
7 Subcellular Fractionation of Plant Tissues: Isolation
of Plastids and Mitochondria
Alyson K. Tobin and Caroline G. Bowsher 53
8 The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds
William S. Pierpoint 65
9 Avoidance of Proteolysis in Extracts
Robert J. Beynon and Simon Oliver (Revised by Paul Cutler) 75
10 Concentration of Extracts
Shawn Doonan 85
11 Making and Changing Buffers
Shawn Doonan 91
12 Purification and Concentration by Ultrafiltration
Paul Schratter (Revised by Paul Cutler) 101
13 Bulk Purification by Fractional Precipitation
Shawn Doonan /17
14 Ion Exchange Chromatography
Chris Selkirk 125
15 Hydrophobic Interaction Chromatography
Paul A. O Farrell 133
16 Affinity Chromatography
Paul Cutler 139
1 7 Dye Ligand Affinity Chromatography
Anne F. McGettrick and D. Margaret Worrall 151
vii
vjji Contents
18 Lectin Affinity Chromatography
Iris West and Owen Goldring (Revised by Paul Cutler) 159
19 Immunoaffinity Chromatography
Paul Cutler 767
20 Immobilized Metal Ion Affinity Chromatography
Tai Tung Yip and T. William Hutchens (Revised by Paul Cutler) 179
21 Chromatography on Hydroxyapatite
Shawn Doonan 191
22 Thiophilic Affinity Chromatography and Related Methods
Paul Matejtschuk 195
23 Affinity Precipitation Methods
lane A. Irwin and Keith F. Upton 205
24 Isoelectric Focusing
Reiner Westermeier 225
25 Chromatofocusing
Timothy J. Mantle and Patricia Noone 233
26 Size Exclusion Chromatography
Paul Cutler 239
27 Fast Protein Liquid Chromatography
David Sheehan and Siobhan O Sullivan 253
28 Reversed Phase Chromatography of Proteins
William A. Neville 259
29 Extraction of Membrane Proteins
Kay Ohlendieck 283
30 Removal of Detergent From Protein Fractions
Kay Ohlendieck 295
31 Purification of Membrane Proteins
Kay Ohlendieck 301
32 Lyophilization of Proteins
Ciaran O Fagain 309
33 Storage of Pure Proteins
Ciaran O Fagain 323
34 Electroelution of Proteins From Polyacrylamide Gels
Michael J. Dunn 339
35 Electroblotting of Proteins From Polyacrylamide Gels
Michael f. Dunn 345
36 Two Dimensional Polyacrylamide Gel Electrophoresis
for Proteome Analyses
Neil A. tones 353
37 Microscale Solution Isoelectrofocusing: A Sample Prefractionation
Method for Comprehensive Proteome Analysis
Xun Zuo and David W. Speicher 361
Contents ix
38 Practical Column Chromatography
Shawn Doonan 377
39 Detection Methods
Jacek Mozdzanowski and Sudhir Burman 389
40 Peptide Proteomics
Dean E. McNulty and /. Randall Slemmon 411
41 Multidimensional Liquid Chromatography of Proteins
Rod Watson and Tim Nadler 425
42 Mass Spectrometry
David J. Bell 447
43 Purification of Therapeutic Proteins
Julian Bonnerjea 455
44 Purification Process Scale Up
Karl Prince and Martin Smith 463
Index 481
|
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| id | DE-604.BV017887300 |
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| indexdate | 2024-07-09T19:22:51Z |
| institution | BVB |
| isbn | 159259655X 1588290670 |
| language | English |
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| physical | XII, 484 S. Ill., graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protein purification protocols ed. by Paul Cutler 2. ed. Totowa, NJ Humana Press 2004 XII, 484 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 244 Proteins Purification Laboratory manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Isolierung Chemie (DE-588)4122223-4 s DE-604 Reinigung (DE-588)4049250-3 s b DE-604 Cutler, Paul Sonstige oth Methods in molecular biology 244 (DE-604)BV035362695 244 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010728119&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein purification protocols Methods in molecular biology Proteins Purification Laboratory manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd Isolierung Chemie (DE-588)4122223-4 gnd |
| subject_GND | (DE-588)4049250-3 (DE-588)4076388-2 (DE-588)4122223-4 (DE-588)4143413-4 |
| title | Protein purification protocols |
| title_auth | Protein purification protocols |
| title_exact_search | Protein purification protocols |
| title_full | Protein purification protocols ed. by Paul Cutler |
| title_fullStr | Protein purification protocols ed. by Paul Cutler |
| title_full_unstemmed | Protein purification protocols ed. by Paul Cutler |
| title_short | Protein purification protocols |
| title_sort | protein purification protocols |
| topic | Proteins Purification Laboratory manuals Proteins isolation & purification Laboratory Manuals Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd Isolierung Chemie (DE-588)4122223-4 gnd |
| topic_facet | Proteins Purification Laboratory manuals Proteins isolation & purification Laboratory Manuals Reinigung Proteine Isolierung Chemie Aufsatzsammlung |
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