Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2003 |
| Beschreibung: | IV, 245 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana |c Yulin Tang |
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Datensatz im Suchindex
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CONTENTS
CONTENTS
ABBREVIATIONS
.
1
SUMMARY
.
4
GENERAL
INTRODUCTION
.
7
PART
I.
SIGNAL
TRANSDUCTION
OF
ABSCISIC
ACID
IN
ARABIDOPSIS
THALIANA.B
1
INTRODUCTION
.
9
1.1
ABA
SIGNAL
TRANSDUCTION
.
9
1.1.1
IDENTIFICATION
OF
ABA
SIGNALING
INTERMEDIATES
.
10
1.1.2
PROTEIN
PHOSPHORYLATION
/DEPHOSPHORYLATION
EVENTS
IN
ABA
SIGNALING
.
13
1.1.3
ABA-REGULATED
GENES
.
17
1.2
HOMEODOMAIN
PROTEIN
.
20
1.2.1
STRUCTURES
AND
PROPERTIES
OF
HOMEODOMAIN
PROTEINS
.
21
1.2.2
FUNCTIONS
OF
HD-ZIP
PROTEINS
.
21
1.2.3
PROTEIN
PHOSPHATASE
ABI1
INTERACTS
WITH
ARABIDOPSIS
THALIANA
HOMEOBOX
PROTEIN
ATHB6
.
22
1.3
YEAST
HYBRID
SYSTEM
IN
PROTEIN
INTERACTION
ASSAY
.
23
1.4
THE
AIM
OF
THIS
WORK
.
26
2
MATERIALS
AND
METHODS
.
29
2.1
MATERIALS
.
29
2.1.1
MICROBE
MATERIALS
.
29
2.1.2
PLANT
MATERIAL
.
29
2.1.3
CDNA
LIBRARIES
.
30
2.1.4
PLASMIDS
.
30
2.1.5
BIOCHEMICALS
AND
REAGENTS
.
34
2.2
METHODS
.
35
2.2.1
CLONING
TECHNIQUES
.
35
2.2.2
CONSTRUCTION
OF
PLASMIDS
.
36
2.2.3
DNA
ANALYSIS
TECHNIQUES
.
39
2.2.4
PROTEIN
ANALYSIS
.
41
2.2.5
CELLULAR
LOCALIZATION
OF
GUS-FUSION
PROTEINS
.
48
2.2.6
TWO-HYBRID
SYSTEM
.
49
2.2.7
THREE-HYBRID
YEAST
SYSTEM
.
53
CONTENTS
2.2.8
AGROBACTERIUM-MEDIATED
PLANT
CELL
TRANSFORMATION
.
53
2.2.9
TRANSIENT
EXPRESSION
IN
PROTOPLASTS
.
54
3
RESULTS
.
59
3.1
IDENTIFICATION
OF
INTERACTION
PARTNERS
OF
ATHB6
.
59
3.1.1
DIFFERENT
VERSIONS
OF
BAIT
PROTEINS
.
59
3.1.2
LIBRARY
SCREENING
WITH
THE
YEAST
TWO-HYBRID
SYSTEM
.
61
3.1.3
ATHB7
AND
ATGLURS
-
-
THE
NEW
INTERACTION
PARTNERS
OF
ATHB6
.
63
3.2
ANALYSIS
OF
THE
INTERACTIONS
BETWEEN
ATHB6,
PP2CS
AND
ATGLURS.
73
3.2.1
TWO-HYBRID
INTERACTION
OF
ATHB6
AND
ABI1/ABI2
.
73
3.2.2
TWO-HYBRID
INTERACTION
OF
ATGLURS
AND
ABI1/ABI2
.
75
3.2.3
PHYSICAL
INTERACTION
BETWEEN
ABI1/
ABI1
AND
ATGLURS
IN
VITRO
.
78
3.2.4
CO-IMMUNOPRECIPITATION
OF
ATGLURS
AND
ABI1
FROM
MAIZE
PROTOPLASTS
IN
V/VO.83
3.2.5
TERNARY
INTERACTION
AMONG
ATHB6,
ATGLURS
AND
A
THIRD
PROTEIN
IN
THREE-HYBRID
YEAST
SYSTEM
.
93
\
3.3
IDENTIFICATION
OF
INTERACTION
PARTNERS
OF
ATGLURS
BY
A
YEAST
TWO-HYBRID
SYSTEM
.
*
.
101
3.3.1
DIFFERENT
VERSIONS
OF
BAIT
PROTEINS
.
101
3.3.2
INTERACTION
PARTNERS
OF
ATGLURS
.102
3.3.3
HOMODIMERIZATION
OF
ATGLURS
IN
YEAST
.
104
3.4
ANALYSIS
OF
THE
FUNCTION
OF
ARABIDOPSIS
ATGLURS
IN
YEAST
.
107
3.4.1
INTERACTIONS
OF
THE
FULL-LENGTH
ATGLURS
WITH
OTHER
PROTEINS
IN
YEAST
.
107
3.4.2
THE
POTENTIAL
OF
ARABIDOPSIS
ATGLURS
TO
SUBSTITUTE
FOR
YEAST
ATGLURS
.109
3.5
EFFECT
OF
ATGLURS
ON
THE
CELLULAR
LOCALIZATION
OF
ATHB6
.
114
3.5.1
CELLULAR
LOCALIZATION
OF
ATGLURS
.
114
3.5.2
ATGLURS
ALTERS
THE
CELLULAR
LOCALIZATION
OF
ATHB6
.
115
3.6
REGULATION
OF
RAB18
TRANSCRIPTION
IN
TRANSIENT
EXPRESSION
SYSTEM
BY
ABI1, ATHB6
AND
ATGLURS
.
120
3.6.1
THE
REPORTER
DRIVEN
BY
RAB18
PROMOTER
IS
ACTIVATED
BY
ABA
.
120
3.6.2
ABH,
ATHB6
AND
ATGLURS
NEGATIVELY
REGULATE
THE
TRANSCRIPTION
OF
RAB18
.
121
3.7
REGULATION
OF
THE
ATHB6-ACTIVATED
TRANSCRIPTION
IN
.
TRANSIENT
EXPRESSION
SYSTEM
.
125
3.7.1
EFFECT
OF
ATHB7
ON
ATHB6-ACTIVATED
TRANSCRIPTION
.127
3.7.2
SUPPRESSION
OF
ATHB6-ACTIVATED
TRANSCRIPTION
BY
ATGLURS
.128
3.7.3
STIMULATION
OF
ATHB6
ACTIVATED
TRANSCRIPTION
BY
ABI1
.133
II
CONTENTS
4
DISCUSSION
.
135
4.1
IDENTIFICATION
OF
PROTEIN
INTERACTION
PARTNERS
BY
YEAST
TWO-HYBRID
SYSTEM
SCREENING
.
135
4.2
INTERACTION
OF
ATGLURS
WITH
ATHB6
AND
PP2C
.
142
4.3
FUNCTION
OF
ATGLURS
.
146
4.4
A
MODEL
FOR
THE
FUNCTIONS
OF
ATGLURS, ATHB6
AND
ABI1
IN
ABA
SIGNALING
.
150
4.5
DOES
ATHB7
REGULATE
THE
GENE
TRANSCRIPTION
AS
THE
ATHB6?
.
152
4.6
IS
ABI1
CONJUGATED
WITH
A
SMALL
PROTEIN
IN
PLANT
.
152
PART
II.
SUPPRESSOR
SCREEN
WITH
AN
AUXIN-RESISTANT
AXR1-12
OF.
ARABIDOPSIS
THALIANA
.
154
1
INTRODUCTION
.
155
1.1
FERTILIZATION
AND
PARTHENOCARPY
.
155
1.2
FRUIT
GROWTH
IS
DEPENDENT
ON
PHYTOHORMONES
SYNTHESIZED
IN
THE
DEVELOPING
SEED
.
155
1.3
ARABIDOPSIS:
AN
ATTRACTIVE
MODEL
PLANT
IN
BIOLOGY
.
157
1.4
SUPPRESSOR
SCREEN
.
160
2
MATERIALS
AND
METHODS
.
161
2.1
PLANT
MATERIALS
AND
GROWTH
CONDITIONS
.
161
2.2
SCREEN
.
161
2.3
DETERMINATION
OF
AUXIN
SENSITIVITY
.
162
2.4
WATER
LOSS
.
162
2.5
GERMINATION
RATIO
.
162
2.6
APICAL
HOOK
AND
ROOT
GRAVITROPISM
.
163
2.7
DNA
MICRO-ISOLATION
FROM
ARABIDOPSIS
LEAVES:
.
163
2.8
PCR
.
163
2.9
GENETIC
ANALYSIS
.
164
3
RESULTS
.
165
3.1
SILIQUE
GROWTH
OF
THE
ORIGINAL
AXR1-12
MUTANT
AND
THE WILD
TYPE
PLANTS
IN
RESPONSE
TO
PGRS
.
165
3.2
ISOLATION
OF
SUPPRESSORS
FROM
AXRL-12
IN
SILIQUE
DEVELOPMENT
.
169
3.3
ROOT
GROWTH
OF
SUPPRESSORS
IN
RESPONSE
TO
2,4-D
.
178
3.4
OTHER
PHYSIOLOGICAL
CHARACTERISTICS
OF
SUPPRESSOR
LINES
.
182
3.5
GENETIC
ANALYSIS
OF
THE
SUPPRESSOR
LINES
.
185
IN
CONTENTS
4
DISCUSSION
.
199
4.1
PLANT
HORMONE
AND
FRUIT
DEVELOPMENT
.
199
4.2
SUPPRESSORS
OF
AXR1-12
.
200
4.3
GRAVITROPISM
OF
SUPPRESSOR
LINES
.
202
4.4
APICAL
HOOK
FORMATION
IN
SUPPRESSOR
LINES
.
204
4.5
SEED
DEVELOPMENT
IN
SUPPRESSOR
LINES
.
205
APPENDIX
.
206
APPENDIX
1.
MEDIA
RECIPES
.
206
APPENDIX
2.
PRIMERS
.
207
APPENDIX
3.
CDNA
AND
AMINO
ACID
SEQUENCES
OF
THE
CANDIDATES
IDENTIFIED
BY
ATHB6
USING
YEAST
TWO-HYBRID
SYSTEM
.
209
APPENDIX
4.
CDNA
AND
AMINO
ACID
SEQUENCES
OF
THE
CANDIDATES
IDENTIFIED
BY
ATGLURS
USING
YEAST
TWO-HYBRID
SYSTEM
.
216
REFERENCES
.
221
REFERENCES
FOR
PART
I
.
.
.
221
REFERENCES
FOR
PART
II
.
236
ACKNOWLEDGEMENTS
.
243
PUBLICATIONS
.
244
CURRICULUM
VITAE
.
245
IV |
| any_adam_object | 1 |
| author | Tang, Yulin 1968- |
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| classification_tum | BIO 480d BIO 450d BIO 498d BIO 220d |
| ctrlnum | (OCoLC)645568658 (DE-599)BVBBV017830211 |
| discipline | Biologie |
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| indexdate | 2025-02-26T17:01:57Z |
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| language | English |
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| publishDateSort | 2003 |
| record_format | marc |
| spelling | Tang, Yulin 1968- Verfasser (DE-588)128706201 aut Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Yulin Tang 2003 IV, 245 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2003 Indolylessigsäure 3- (DE-588)4242054-4 gnd rswk-swf Abscisinsäure (DE-588)4141106-7 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Indolylessigsäure 3- (DE-588)4242054-4 s Signaltransduktion (DE-588)4318717-1 s DE-604 Abscisinsäure (DE-588)4141106-7 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010702807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tang, Yulin 1968- Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Indolylessigsäure 3- (DE-588)4242054-4 gnd Abscisinsäure (DE-588)4141106-7 gnd Ackerschmalwand (DE-588)4141299-0 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4242054-4 (DE-588)4141106-7 (DE-588)4141299-0 (DE-588)4318717-1 (DE-588)4113937-9 |
| title | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana |
| title_auth | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana |
| title_exact_search | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana |
| title_full | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Yulin Tang |
| title_fullStr | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Yulin Tang |
| title_full_unstemmed | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana Yulin Tang |
| title_short | Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana |
| title_sort | analysis of auxin and abscisic acid signal transduction in arabidopsis thaliana |
| topic | Indolylessigsäure 3- (DE-588)4242054-4 gnd Abscisinsäure (DE-588)4141106-7 gnd Ackerschmalwand (DE-588)4141299-0 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Indolylessigsäure 3- Abscisinsäure Ackerschmalwand Signaltransduktion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010702807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT tangyulin analysisofauxinandabscisicacidsignaltransductioninarabidopsisthaliana |