The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Univ., Diss., 2003 |
| Beschreibung: | 76 S. graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1807772352819232768 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
1.
INTRODUCTION
1.1
HEMATOPOIESIS
1
1.2
TRANSCRIPTION
FACTORS
INVOLVED
IN
HEMATOPOIESIS
2
1.3
MYELOPOIESIS
5
1.4
TRANSCRIPTION
FACTORS
6
1.4.1
PU.L
8
1.4.2
C/EBPA
11
1.4.3
C-JUN
13
1.5
AIM
OF
THE
STUDY
15
2.
MATERIALS
2.1
REAGENTS
16
2.2
RADIOACTIVE
SUBSTANCES
17
2.3
ENZYMES
17
2.4
REAGENT
KITS
17
2.5
STANDARDS,
MARKERS
AND
LADDERS
18
2.6
MISCELLANEOUS
18
2.7
BIOLOGICAL
MATERIAL
18
2.7.1
BACTERIA
18
2.7.2
MAMMALIAN
CELL
LINES
18
2.8
PLASMIDS
19
2.9
BUFFERS
19
2.10
GROWTH
MEDIA
2.10.1
BACTERIA
21
2.10.2
MAMMALIAN
CELL
CULTURE
21
3.
METHODS
3.1
TRANSIENT
TRANSFECTION
OF
ADHERENT
EUKARYOTIC
CELLS
BY
LIPOFECTAMINE
22
3.2
TRANSIENT
TRANSFECTION
OF
ADHERENT
EUKARYOTIC
CELLS
BY
EFFECTENE
23
3.3
IN
VITRO
TRANSCRIPTION
AND
TRANSLATION
24
3.4
GST
EXPRESSION
OF
PROTEINS
FUSED
TO
GST
25
3.5
CO-IMMUNOPRECIPITATION
26
3.6
TRANSDUCTIONS
3.6.1
PRODUCTION
OF
RETROVIRUS
26
3.6.2
TRANSDUCTION
OF
CD34
+
CELLS
27
3.6.3
IN
VITRO
LIQUID
CULTURE
27
3.7
IMMUNOLOCALIZATION
28
3.8
REAL
TIME
POLYMERASE
CHAIN
REACTION
28
4
RESULTS
4.1
C/EBPA
BLOCKS
THE
TRANSCRIPTIONAL
ACTIVITY
OF
PU.
1
ON
A
MINIMAL
TK
PROMOTER
CONTAINING
PU.
1
DNA
BINDING
SITES
ONLY
31
4.2
C/EBPA
PHYSICALLY
INTERACTS
WITH
PU.
1
35
4.3
C/EBPA
INHIBITS
C-JUN
CO-ACTIVATION
OF
PU.L
35
4.4
C/EBPA
DISPLACES
C-JUN
FROM
BINDING
TO
PU.
1
IN
VITRO
36
4.5
C/EBPA
INTERACTS
WITH
PU.
1
VIA
ITS
LEUCINE
ZIPPER
IN
THE
DNA
BINDING
DOMAIN
40
4.6
C/EBPA
DOES
NOT
RECRUIT
TSA-SENSITIVE
CO-REPRESSORS
IN
DOWNREGULATING
PU.
1
'S
TRANSCRIPTIONAL
ACTIVITY
43
4.7
C/EBPA
DOWNREGULATES
PU.
1
EXPRESSION
IN
MYELOID
U937
CELLS
44
4.8
C/EBPA
INHIBITS
PU.L
INDUCED
DENDRITIC
CELL
DEVELOPMENT
44
5
DISCUSSION
51
6
SUMMARY
58
ZUSAMMENFASSUNG
59
7
REFERENCES
60 |
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| spelling | Reddy, Venkateshwar A. 1975- Verfasser (DE-588)128354135 aut The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions von Venkateshwar A Reddy 2002 76 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2003 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010610154&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Reddy, Venkateshwar A. 1975- The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions |
| subject_GND | (DE-588)4113937-9 |
| title | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions |
| title_auth | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions |
| title_exact_search | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions |
| title_full | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions von Venkateshwar A Reddy |
| title_fullStr | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions von Venkateshwar A Reddy |
| title_full_unstemmed | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 possible role in lineage commitment decisions von Venkateshwar A Reddy |
| title_short | The granulocytic inducer C/EBPAlpha inactivates the myeloid master regulator PU.1 |
| title_sort | the granulocytic inducer c ebpalpha inactivates the myeloid master regulator pu 1 possible role in lineage commitment decisions |
| title_sub | possible role in lineage commitment decisions |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010610154&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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