Regulation of neurogenesis in the adult mammalian brain:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Regensburg
2003
|
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext Inhaltsverzeichnis |
| Beschreibung: | 162 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Regulation of neurogenesis in the adult mammalian brain |c vorgelegt von Christiana Marie Cooper-Kuhn |
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Datensatz im Suchindex
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| adam_text |
INDEX
REGULATION OF NEUROGENESIS IN THE ADULT MAMMALIAN BRAIN.1
LIST OF ABBREVIATIONS.8
INTRODUCTION.10
GENERATION OF NEW NEURONS IN THE ADULT MAMMALIAN BRAIN
.10
SUBVENTRICULAR ZONE/OLFACTORY BULB NEUROGENESIS
.11
HIPPOCAMPAL NEUROGENESIS
.13
DEVELOPMENTAL ASPECTS OF ADULT NEUROGENESIS
.15
PHYLOGENETIC ASPECTS OF ADULT NEUROGENESIS.17
INVERTEBRATES
.17
NON-MAMMALIAN VERTEBRATES
.17
MAMMALS
.18
FUNDAMENTALS OF ADULT NEUROGENESIS
.19
REGULA TING ADULT NEUROGENESIS: WHICH STIMULI MAKE A DIFFERENCE?
.23
SYSTEMIC INFLUENCES:
.23
EXTRACELLULAR CUES:
.25
OVERALL GOAL.27
SPECIFIC AIMS.27
OVERVIEW.29
EXPERIMENTS.30
EXPERIMENT I - CRITICAL EVALUATION OF BRDU LABELING.30
OBJECTIVE. 30
INTRODUCTION TO EXPERIMENT.30
EXPERIMENTAL DESIGN.32
RESULTS AND DISCUSSION.32
SUMMARY.41
EXPERIMENT II - IMPAIRED ADULT NEUROGENESIS IN MICE LACKING THE
TRANSCRIPTION
FACTOR E2F1.42
OBJECTIVE.42
INTRODUCTION TO EXPERIMENT.43
EXPERIMENTAL DESIGN.45
RESULTS.45
LOSS OF E2F1 AFFECTS SIZE OF ADULT MICE AND BRAIN WEIGHT.
.45
NEUROANATOMY OF ADULT E2F1 -DEFICIENT ANIMALS.
.46
4
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/968915698
LOSS OFE2F1 LEADS TO LESS PROLIFERATION IN DENTATE GYRUS AND
SUBVENTRICULAR ZONE
.48
LOSS OF E2F1 RESULTS IN SIGNIFICANT DECREASE OF NEURONS IN DENTATE GYRUS
AND OLFACTORY BULB
.50
LOSS OF E2F1 RESULTS IN DECREASED CELL DEATH IN DENTATE GYRUS AND
OLFACTORY BULB GRANULE CELL LAYERS.
.51
DISCUSSION.53
FUNCTIONAL SIGNIFICANCE OFE2F1 IN OTHER ANIMAL MODELS
.53
CELL DEATH IN REGIONS OF ADULT NEUROGENESIS
.53
POSTNATAL PHENOTYPE OF E2F1-DEFICIENCY
.54
EXPERIMENTS III & IV - NEUROTRANSMITTERS AND THEIR POSITION IN THE GAME
OF ADULT
NEUROGENESIS.56
EXPERIMENT III - CHOLINERGIC DENERVATION LESION.57
OBJECTIVE.57
INTRODUCTION TO EXPERIMENT.58
EXPERIMENTAL DESIGN. 59
RESULTS
.60
ICV INJECTIONS OF 192LGG-SAPORIN RESULT IN SELECTIVE ELIMINATION OF
CHOLINERGIC NEURONS IN MEDIAL
SEPTUM
.60
DECREASE OF NEUROGENESIS IN DENTATE GYRUS AFTER CHOLINERGIC DENERVATION
.61
CHOLINERGIC DENERVATION CAUSES INCREASE IN NON-NEURONAL CELLS IN THE
DENTATE GYRUS
.62
CHOLINERGIC DENERVATION CAUSES AN INCREASE IN CELL DEATH IN THE DENTATE
GYRUS.
.62
DECREASE OF NEUROGENESIS IN OLFACTORY BULB AFTER CHOLINERGIC DENERVATION
.63
CHOLINERGIC DENERVATION CAUSES AN INCREASE IN CELL DEATH IN THE
PERIGLOMERULAR LAYER
.65
DISCUSSION
.65
EXPERIMENT IV - NORADRENERGIC DENERVATION LESION.68
OBJECTIVE.68
INTRODUCTION TO EXPERIMENT.68
EXPERIMENTAL DESIGN.69
RESULTS
.69
DECREASE OF NEUROGENESIS IN DENTATE GYRUS AFTER NORADRENERGIC
DENERVATION
.70
NORADRENERGIC DENEN/ATION CAUSES DECREASE IN NON-NEURONAL CELLS IN THE
DENTATE GYRUS
.71
CELL DEATH IN THE DENTATE GYRUS IS NOT ALTERED BY NORADRENERGIC
DENERVATION
.71
INCREASED PERIGLOMERULAR NEUROGENESIS IN OLFACTORY BULB AFTER
NORADRENERGIC DENERVATION
.72
NORADRENERGIC DENERVATION
CAUSES A DECREASE IN CELL DEATH IN THE OLFACTORY GRANULE CELL LAYER.73
DISCUSSION
.74
EXPERIMENT V - VASCULAR ENDOTHELIAL GROWTH FACTOR INDUCES NEUROGENESIS
IN
THE ADULT BRAIN.76
5
OBJECTIVE.76
INTRODUCTION TO EXPERIMENT.76
EXPERIMENTAL DESIGN.78
RESULTS AND DISCUSSION.78
VEGF STIMULATES OLFACTORY NEUROGENESIS
.78
VEGF STIMULATES HIPPOCAMPAL NEUROGENESIS
.79
VEGF DECREASES CELL DEATH IN SVZ/OB AND HIPPOCAMPUS
.80
VEGF DOES NOT STIMULATE PROLIFERATION OF GLIAL CELLS
.81
VEGF DOES NOT INDUCE NEOVASCULARIZATION OF THE VENTRICLE WALL OR THE
DENTATE GYRUS
.81
EXPERIMENT VI - TRANSIENT EXPRESSION OF DOUBLECORTIN DURING ADULT
NEUROGENESIS.83
OBJECTIVE.83
INTRODUCTION TO EXPERIMENT.83
EXPERIMENTAL DESIGN. 84
RESULTS.84
DOUBLECORTIN EXPRESSION DURING ADULT HIPPOCAMPAL NEUROGENESIS
.84
DOUBLECORTIN EXPRESSION IN THE AGING DENTATE GYRUS
.88
DOUBLECORTIN EXPRESSION IN THE SVZ/OB SYSTEM
.89
DOUBLECORTIN EXPRESSION IN THE AGING OLFACTORY BULB
.92
DISCUSSION.92
OCX
EXPRESSION IN ADULT NEURAL PRECURSOR CELLS?.93
DCX EXPRESSION IN MATURE NEURONS?
.94
OCX, A
TRANSIENT MARKER FOR NEURONAL COMMITTED PRECURSOR CELLS.95
CONCLUDING DISCUSSION.97
NEUROGENESIS: A BALANCE OF PROLIFERATION AND APOPTOSIS.99
COMPARISON OF THE DIFFERENT EXPERIMENTAL STRATEGIES:.100
DISEASE-RELATED MODELS AND THE CLINICAL PERSPECTIVE.101
TRAUMATIC BRAIN LESION
.101
EPILEPSY/SEIZURE
.102
HYPOXIA/ISCHEMIA
.102
OUTLOOK.104
MATERIALS AND METHODS.106
ANIMALS, INJECTIONS, AND TISSUE PREPARATION.106
EXPERIMENT I - CRITICAL EVALUATION OFBRDU LABELING
.106
EXPERIMENT II - IMPAIRED ADULT NEUROGENESIS IN MICE LACKING THE
TRANSCRIPTION FACTOR E2F1
.106
EXPERIMENT III - CHOLINERGIC DENERVATION LESION
.107
6
EXPERIMENT IV - NORADRENERGIC DENERVATION LESION
.
EXPERIMENT V - VASCULAR ENDOTHELIAL GROWTH FACTOR INDUCES NEUROGENESIS
IN THE ADULT BRAIN
EXPERIMENT VI - TRANSIENT EXPRESSION OF DOUBLECORTIN DURING ADULT
NEUROGENESIS
.
HISTOLOGICAL PROTOCOLS.
BRDU-DAB PROTOCOL.
BRDU TRIPLE IMMUNOFLUORESCENCE PROTOCOL
.
IMMUNOFLUORESCENCE PROTOCOL
.
STANDARD TUNEL PROTOCOL
.
TUNEL + IMMUNOFLUORESCENCE PROTOCOL
.
TUNEL + BRDU PROTOCOL.
.
NISSL STAINING
.
DAPI/PROPLDIUM IODIDE STAINING
.
ANTIBODIES.
DNA PURIFICATION.
PCR.
STEREOLOGY.
ADAPTATIONS FOR SPECIFIC EXPERIMENTS
.
MICROSCOPY.
NEURONAL DIFFERENTIATION.
STATISTICAL ANALYSIS.
CHEMICALS.
APPENDIX.
TABLE I.
INTERVENTIONS INFLUENCING NEUROGENESIS IN DENTATE GYRUS
.
CURRICULUM VITAE.
BIBLIOGRAPHY.
107
107
108
109
109
110
110
111
111
112
112
113
114
115
115
115
118
119
119
119
121
125
125
126
128
132
ACKNOWLEDGEMENTS
161 |
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| spelling | Cooper-Kuhn, Christiana Marie Verfasser aut Regulation of neurogenesis in the adult mammalian brain vorgelegt von Christiana Marie Cooper-Kuhn Regensburg 2003 162 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Regensburg 2003 Histochemie (DE-588)4025087-8 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Hippocampus (DE-588)4642127-0 gnd rswk-swf Neurogenese (DE-588)4331352-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s Hippocampus (DE-588)4642127-0 s Neurogenese (DE-588)4331352-8 s Genregulation (DE-588)4122166-7 s Histochemie (DE-588)4025087-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-opus-2872 https://nbn-resolving.org/urn:nbn:de:bvb:355-opus-2872 Resolving-System kostenfrei Volltext https://epub.uni-regensburg.de/10120/ Verlag kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010536266&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cooper-Kuhn, Christiana Marie Regulation of neurogenesis in the adult mammalian brain Histochemie (DE-588)4025087-8 gnd Genregulation (DE-588)4122166-7 gnd Maus (DE-588)4169148-9 gnd Hippocampus (DE-588)4642127-0 gnd Neurogenese (DE-588)4331352-8 gnd |
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| title | Regulation of neurogenesis in the adult mammalian brain |
| title_auth | Regulation of neurogenesis in the adult mammalian brain |
| title_exact_search | Regulation of neurogenesis in the adult mammalian brain |
| title_full | Regulation of neurogenesis in the adult mammalian brain vorgelegt von Christiana Marie Cooper-Kuhn |
| title_fullStr | Regulation of neurogenesis in the adult mammalian brain vorgelegt von Christiana Marie Cooper-Kuhn |
| title_full_unstemmed | Regulation of neurogenesis in the adult mammalian brain vorgelegt von Christiana Marie Cooper-Kuhn |
| title_short | Regulation of neurogenesis in the adult mammalian brain |
| title_sort | regulation of neurogenesis in the adult mammalian brain |
| topic | Histochemie (DE-588)4025087-8 gnd Genregulation (DE-588)4122166-7 gnd Maus (DE-588)4169148-9 gnd Hippocampus (DE-588)4642127-0 gnd Neurogenese (DE-588)4331352-8 gnd |
| topic_facet | Histochemie Genregulation Maus Hippocampus Neurogenese Hochschulschrift |
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