The role of complement and DNase I in the opsonisation and clearance of dying cells: implications for the etiopathogenesis of systemic Lupus erythematosus (SLE)
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Erlangen-Nürnberg, Univ., Diss., 2003. - Enth. Zsfassung in engl. und dt. Sprache |
| Beschreibung: | 86 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The role of complement and DNase I in the opsonisation and clearance of dying cells |b implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) |c von Udo Sebastian Gaipl |
| 264 | 1 | |c 2003 | |
| 300 | |a 86 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2003 | ||
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Datensatz im Suchindex
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|---|---|
| adam_text |
1
ZUSAMMENFASSUNG/SUMMARY
1
2
INTRODUCTION
3
2.1
APOPTOSIS
3
2.2
NECROSIS
7
2.3
COMPLEMENT
10
2.4
CLEARANCE
OF
DYING
CELLS
11
2.5
AUTOIMMUNITY
12
2.6
SCOPE
OF
THE
THESIS
14
3
RESULTS
15
3.1
COMPLEMENT
DOES
NOT
CHANGE
THE
MORPHOLOGY
OF
CULTURED
PBL
AND
PMN
15
3.2
TIME
COURSE
OF
APOPTOSIS
AND
NECROSIS
18
3.3
COMPLEMENT
MAINLY
BINDS
TO
SUBCELLULAR
FRAGMENTS
GENERATED
DURING
IN
VITRO
CULTURE
OF
PMN
22
3.4
COMPLEMENT
BINDS
SUBCELLULAR
FRAGMENTS
OF
PBL
GENERATED
DURING
VERY
LATE
PHASES
OF
APOPTOSIS
25
3
5
AUTOLOGOUS
SERUM
DEGRADES
NUCLEI
DERIVED
FROM
NECROTIC
CELLS
30
3.6
DNASE
I
IS
CAPABLE
TO
DEGRADE
NECROTIC
CELL-DERIVED
CHROMATIN
34
3.7
THE
DEGRADATION
CAPACITY
OF
SERUM
CAN
BE
INHIBITED
BY
EDTA
AND
ZN
2
*
IONS
35
3.8
THE
DEGRADATION
OF
NECROTIC
CELL-DERIVED
CHROMATIN
IS
INDEPENDENT
OF
CASPASES
37
3.9
C1Q
AUGMENTS
THE
DNASE
L-MEDIATED
DEGRADATION
OF
NECROTIC
CELL-DERIVED
CHROMATIN
38
3.10
TOTAL
CLASSICAL
COMPLEMENT
ACTIVITY
OF
SERA
FROM
SLE
PATIENTS
CORRELATES
WITH
THE
DEGRADATION
OF
NUCLEI
FROM
NECROTIC
CELLS
41
3.11
SERA
FROM
SLE
PATIENTS
HAVE
LOWER
DEGRADATION
CAPACITY
FOR
NECROTIC
CELLS
IN
COMPARISON
TO
SERA
FROM
PATIENTS
WITH
RHEUMATOID
ARTHRITIS
(RA)
42
3.12
C1Q
IS
ESSENTIAL
FOR
AN
EFFECTIVE
CLEARANCE
OF
DEGRADED
NECROTIC
CELL-DERIVED
CHROMATIN
BY
ACTIVATED
MONOCYTES
43
3.13
REDUCED
IN
VITRO
PHAGOCYTOSIS
OF
APOPTOTIC
MATERIAL
BY
MACROPHAGES
FROM
SLE
PATIENTS
46
3.14
ACCUMULATION
OF
UNINGESTED
APOPTOTIC
CELLS
IN
GERMINAL
CENTERS
OF
SLE
PATIENTS
47
3.15
ASSOCIATION
OF
APOPTOTIC
NUCLEI
WITH
FOLLICULAR
DENDRITIC
CELLS
(FDC)
IN
GERMINAL
CENTERS
OF
SLE
PATIENTS
48
4
DISCUSSION
52
4.1
COMPLEMENT
BINDING
OF
CELLS
UNDERGOING
APOPTOSIS
OR
NECROSIS
52
4.2
C1
Q
AND
DNASE
I
CO-OPERATE
IN
THE
DEGRADATION
OF
NECROTIC
CELL-DERIVED
CHROMATIN
54
4.3
C1
Q
IS
NECESSARY
FOR
AN
EFFECTIVE
UPTAKE
OF
NECROTIC
CELL-DERIVED
CHROMATIN
57
4.4
IMPAIRED
CLEARANCE
OF
APOPTOTIC
CELLS
IN
GC
OF
SOME
SLE
PATIENTS
58
4.5
HYPOTHESIS
FOR
THE
ETIOLOGY
OF
AUTOIMMUNITY
60
4.6
OUTLOOK
63
5
MATERIALS
AND
METHODS
64
5.1
MATERIALS
64
5.2
PATIENTS,
CELLS,
AND
SERA
68
5.3
INHIBITORS
69
5.4
CH100
DETECTION
69
5.5
INDUCTION
AND
DETECTION
OF
APOPTOSIS
AND
NECROSIS
70
5.6
COMPLEMENT
BINDING
ASSAY
71
5.7
CHROMATIN
DEGRADATION
ASSAY
71
5.8
ANALYSIS
OF
POLYNUCLEOSOME
DEGRADATION
BY
AGAROSE
GEL
ELECTROPHORESIS
71
5.9
PHAGOCYTOSIS
ASSAY
72
5.10
ANALYSES
OF
NUCLEAR
DEGRADATION
AND
COMPLEMENT
BINDING
BY
FLOW
CYTOMETRY
72
5.11
IMMUNOHISTOCHEMISTRY
AND
IMMUNOFLUORESCENCE
OF
GC
72
5.12
CONFOCAL
MICROSCOPY
73
5.13
EVALUATION
OF
INGESTED
AND
FREE
APOPTOTIC
NUCLEI
IN
GC
73
5.14
STATISTICAL
ANALYSIS
73
6
LITERATURE
74
7
ABBREVIATION
INDEX 85 |
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| author | Gaipl, Udo Sebastian 1973- |
| author_GND | (DE-588)124844979 |
| author_facet | Gaipl, Udo Sebastian 1973- |
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| author_sort | Gaipl, Udo Sebastian 1973- |
| author_variant | u s g us usg |
| building | Verbundindex |
| bvnumber | BV017312843 |
| classification_rvk | YF 4200 |
| ctrlnum | (OCoLC)249209146 (DE-599)BVBBV017312843 |
| discipline | Medizin |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV017312843 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:25:46Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010436622 |
| oclc_num | 249209146 |
| open_access_boolean | |
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| owner_facet | DE-29 DE-29T DE-12 DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | 86 S. Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| record_format | marc |
| spelling | Gaipl, Udo Sebastian 1973- Verfasser (DE-588)124844979 aut The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) von Udo Sebastian Gaipl 2003 86 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2003. - Enth. Zsfassung in engl. und dt. Sprache Erlangen-Nürnberg, Univ., Diss., 2003 Komplement Immunologie (DE-588)4120589-3 gnd rswk-swf Pathogenese (DE-588)4115512-9 gnd rswk-swf Erythematodes (DE-588)4015455-5 gnd rswk-swf Zelltod (DE-588)4207415-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Erythematodes (DE-588)4015455-5 s Pathogenese (DE-588)4115512-9 s Zelltod (DE-588)4207415-0 s Komplement Immunologie (DE-588)4120589-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010436622&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gaipl, Udo Sebastian 1973- The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) Komplement Immunologie (DE-588)4120589-3 gnd Pathogenese (DE-588)4115512-9 gnd Erythematodes (DE-588)4015455-5 gnd Zelltod (DE-588)4207415-0 gnd |
| subject_GND | (DE-588)4120589-3 (DE-588)4115512-9 (DE-588)4015455-5 (DE-588)4207415-0 (DE-588)4113937-9 |
| title | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) |
| title_auth | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) |
| title_exact_search | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) |
| title_full | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) von Udo Sebastian Gaipl |
| title_fullStr | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) von Udo Sebastian Gaipl |
| title_full_unstemmed | The role of complement and DNase I in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) von Udo Sebastian Gaipl |
| title_short | The role of complement and DNase I in the opsonisation and clearance of dying cells |
| title_sort | the role of complement and dnase i in the opsonisation and clearance of dying cells implications for the etiopathogenesis of systemic lupus erythematosus sle |
| title_sub | implications for the etiopathogenesis of systemic Lupus erythematosus (SLE) |
| topic | Komplement Immunologie (DE-588)4120589-3 gnd Pathogenese (DE-588)4115512-9 gnd Erythematodes (DE-588)4015455-5 gnd Zelltod (DE-588)4207415-0 gnd |
| topic_facet | Komplement Immunologie Pathogenese Erythematodes Zelltod Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010436622&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT gaipludosebastian theroleofcomplementanddnaseiintheopsonisationandclearanceofdyingcellsimplicationsfortheetiopathogenesisofsystemiclupuserythematosussle |