P450-dependent alkane monooxygenase from Acinetobacter sp. EB104: in vitro model for the enzymatic oxidation of hydrocarbons
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Leipzig, Univ., Diss., 2003 |
| Beschreibung: | 123, 6 Bl. Ill., graph. Darst. |
Internformat
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| 049 | |a DE-91 |a DE-355 | ||
| 100 | 1 | |a Gupta, Nishith |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 |b in vitro model for the enzymatic oxidation of hydrocarbons |c by Nishith Gupta |
| 264 | 1 | |c 2003 | |
| 300 | |a 123, 6 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Leipzig, Univ., Diss., 2003 | ||
| 650 | 0 | 7 | |a Enzymatische Oxidation |0 (DE-588)4468528-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Alkan-Monooxygenase |g Alkan-1-Monooxygenase |0 (DE-588)4738811-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Acinetobacter |0 (DE-588)4278742-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Alkane |0 (DE-588)4191827-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Cytochrom P-450 |0 (DE-588)4148705-9 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Acinetobacter |0 (DE-588)4278742-7 |D s |
| 689 | 0 | 1 | |a Cytochrom P-450 |0 (DE-588)4148705-9 |D s |
| 689 | 0 | 2 | |a Alkan-Monooxygenase |g Alkan-1-Monooxygenase |0 (DE-588)4738811-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Acinetobacter |0 (DE-588)4278742-7 |D s |
| 689 | 1 | 1 | |a Alkane |0 (DE-588)4191827-7 |D s |
| 689 | 1 | 2 | |a Enzymatische Oxidation |0 (DE-588)4468528-2 |D s |
| 689 | 1 | 3 | |a Cytochrom P-450 |0 (DE-588)4148705-9 |D s |
| 689 | 1 | |5 DE-604 | |
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Datensatz im Suchindex
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TABLE OF CONTENTS
1. INTRODUCTION.1
1.1 INTRODUCTION TO BIOCATA LYSIS.1
1.1.1 IMPORTANCE OF BIOCATAL YSIS.1
1.1.2 WHOLE CELLS
VERSUS PURE ENZYMES.1
1.1.3 OXIDATIVE BIOTRANSFORMATION.1
1.2 OVERVIEW OF OXYGENASES.2
1.2.1 CLASSIFICATION OF OXYGENASES.2
1.2.2 NON-HEME IRON MONOOX YGENASES.3
1.3 CYTOCHROME P450-DEPENDENT SYSTEMS.4
1.3.1 DISTRIBUTION, NOMENCLATURE AND FUNCTIONAL DIVERSITY OF P450S.4
1.3.2 REACTION MECHANISM OF P450S AND THEIR PARTNER-PROTEINS.6
1.3.3 MOLECULAR FEATURES OF P450.9
1.3.4 MOLECULAR FEATURES OF THE ELECTRON-TRANSFER PROTEINS.11
1.3.4.1 CHARACTERISTICS OF FERREDOXIN.11
1.3.4.2 CHARACTERISTICS OF REDUCTASE. 13
1.3.5 MOLECULAR RECOGNITION IN P450 SYSTEMS.14
1.3.5.1 INTERACTIONS BETWEEN THE PARTNER-PROTEINS.14
1.3.5.2 INTERACTIONS BETWEEN P450 AND LIPIDS.14
1.4 ALKANE METABOLISM IN NATURE.15
1.4.1 NON-HEME ALKANE HYDROXYLASES.15
1.4.2 P450-DEPENDENT IKANE OXIDIZING SYSTEMS.16
1.4.3 P450-DEPENDEM ALKANE OXIDATION IN
ACINETOBACTER 17
1.5 AIMS OF THIS DISSERTATION AND SURVEY OF ITS CONTENTS.18
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/968889425
2 MATERIALS AND METHODS.1?
2.1 CHEMICALS.1?
2.2 INSTRUMENTS AND CONSUMABLES.21
2.3 MICROBIOLOGICAL METHODS FOR
ACINETOBACTER SP. EB104.23
2.3.1 MAINTENANCE OF THE BACTERIAL STRAIN.23
2.3.2 CULTIVATION OF BIOMASS AND PREPARATION OF THE CELL-FREE
EXTRACTS.23
PREPARATION OF INOCULUMS
CULTIVATION IN SHAKING FLASKS
CULTIVATION IN BATCH FERMENTOR
PREPARATION OF THE CELL-FREE EXTRACTS
2.4 CHROMATOGRAPHIC METHODS.24
2.4.1 ION-EXCHANGE CHROMATOGRAPHY OF THE CELL-FREE EXTRACTS ON
DEAE-SEPHAROSE.25
2.4.2
FURTHER PURIFICATION OF THE
P450NON
SYSTEM'S COMPONENTS.
25
2.4.2.1
PURIFICATION OF
P450NON.25
GEL FILTRATION ON SEPHACRYL HR100
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
GEL FILTRATION ON SUPERDEX 75
REMOVAL OF TWEEN
80
FROM THE
P450NON
PREPARATION ON DEAE-SEPHAROSE
2.4.2.2 PURIFICATION OF NONADOXIN REDUCTASE.26
GEL FILTRATION ON SUPERDEX 75
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
2.4.2.3 PURIFICATION OF NONADOXIN.26
GEL FILTRATION ON SUPERDEX 75
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
2.5 SPECTROSCOPY.27
2.5.1 OPTICAL ABSORB ;NCE BASED METHODS.27
2.5.2
MEASUREMENT OF THE ABSORBANCE DIFFERENCE SPECTRA OF
P450NON 27
2.5.2.1
ESTIMATION OF
P450YYON
BY ITS CO DIFFERENCE SPECTRA.
27
2.5.2.2
RECORDING OF THE D IFFERENCE SPECTRA OF
P450NON
WITH DETERGENTS.
28
2.5.2.3
RECORDING OF THE D IFFERENCE SPECTRA OF
P450NON
WITH ITS PUTATIVE SUBSTRATES.
28
2.5.3 PHOTOMETRIC ASSAYS FOR NONADOXIN AND NONADOXIN REDUCTASE.28
CYTOCHROME C-REDUCTION ASSAY
HEXACYANOFERRATE-III (HCF)-REDUCTION ASSAY
2,6-DICHLOROPHENOLI NDOPHENOL-REDUCTION ASSAY
2.5.4 ANALYSIS OF THE FLAVIN MOIETY FROM NONADOXIN REDUCTASE.2?
2.5.5 PROTEIN ESTIMATION.29
2.5.6 FLUOROMETRY.2?
IDENTIFICATION OF THE FLAVIN MOIETY OF NONADOXIN REDUCTASE
2.5.7
ELECTRON PARAMAGNETIC RESONANCE (EPR)-BASED METHODS.30
EPR
SPECTRA OF
P450NON
AND NONADOXIN
INTERACTION BETWEEN
P450NON
AND NONADOXIN
EPR SPECTRA OF
P450NON
IN THE PRESENCE OF SUBSTRATES AND DETERGENTS
2.6 ELECTROPHORESIS.31
SODIUM DODECYL SULFATE POLY-ACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)
STAINING OF SDS-PAGE GELS
ISO ELECTRIC FOCUSSING (IEF)
STAINING OF IEF GELS
2.7
MONITORING OF MOLECULAR RECOGNITION IN
P450NOYY
SYSTEM BY OPTICAL BIOSENSO RS.33
2.7.1 INTERACTIONS BETWEEN THE LIPOSOMES AND PROTEINS.33
LIPOSOME PREPARATION AND IMMOBILIZATION
INTERACTION PROCEDURES
DATA ANALYSES
2.7.2
INTERACTIONS BETWEEN THE PROTEINS.34
IMMOBILIZATION OF
P450NON,
NONADOXIN REDUCTASE AND NONADOXIN
INTERACTION PROCEDURES
DATA ANALYSES
2.8 PROCEDURES FOR THE RECONSTITUTION OF THE ENZYMAT IC ACTIVITY.36
2.8.1 NADH-OXIDATION ASSAY.36
2.8.2 RADIOMETRIC METHODS.36
2.8.2.1 REACTION SET-UP AND THE PRODUCT EXTRACTION.36
2.8.2.2 PHOSPHOR-IMAGING AND DATA ANALYSIS.37
2.8.2.3 PHOSPHOLIPID ESICLES AND MICELLE PREPARATION.38
3 RESULTS.-59
3.1 PRODUCTION OF THE P450NON-ENRICHED
ACINETOBACTER SP. EB104 BIOMASS.39
INDUCTION OF P450
YY
M AND BIOMASS GROWTH
DISTRIBUTION OF THE
P450NON
SYSTEM'S COMPONENTS IN THE SUB-CELLULAR FRACTIONS
3.2 PURIFICATION OF THE
P450NON
SYSTEM.41
3.2.1
SEPARATION OF THE
P450NON
SYSTEM'S COMPONENTS FROM THE CELL-FREE EXTRACTS.
42
3.2.2 FURTHER PURIFICATION OF THE
P450NON
SYSTEM.
44.
3.2.2.1 PURIFICATION
OF
P450NON
.
44.
GEL FILTRATION ON SEPHACRYL HR100
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
GEL FILTRATION ON SUPERDEX 75
REMOVAL OF TWEEN
80
FROM THE
P450NON
PREPARATION ON DEAE-SEPHAROSE
EVALUATION OF THE PURITY OF
P450N M
3.2.2.2 PURIFICATION OF NONADOXIN.4.8
GEL FILTRATION ON SUPERDEX 75
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
EVALUATION OF THE PURITY OF NONADOXIN
3.2.2.3 PURIFICATION OF NONADOXIN REDUCTASE.51
GEL FILTRATION ON SUPERDEX 75
ION-EXCHANGE CHROMATOGRAPHY ON DEAE-SEPHAROSE
EVALUATION OF THE PURITY OF NONADOXIN REDUCTASE
3.3
PHYSICAL AND CHEMICAL PROPERTIES OF THE
P450NON
SYSTEM'S COMPONENTS.
53
3.3.1 PROPERTIES OF
P450NON.
55
3.3.1.1 OPTICAL FEATURES OF P450
NON AND ITS AGGREGATION.53
ABSOLUTE ABSORPTION SPECTRA OF THE PURIFIED
P450M N
SPECTRAL SHIFTS AD AGGREGATION OF
P450NON
3.3.1.2 EPR-BASED WADIES ON THE HEME ENVIRONMENT OF
P450NON
AND ITS AGGREGATION.
56
3.3.1.3 STABILITY OT
P450 NON
. .
.
3.3.1.4 INTERACTION BETWEEN LIPOSOMES AND
P450NON
.
59
3.3.2 PHYSICO-CHEMICAL PROPERTIES OF THE ELECTRON-TRANSFER PROTEINS.61
3.3.2.1 SPECTRAL PROPERTIES OF NONADOXIN AND ITS STABILITY.61
OPTICAL ABSORPTION SPECTRA OF NONADOXIN
EPR SPECTRA OF NONADOXIN
STABILITY OF NONADOXIN
3.3.2.2 ABSORBANCE AND FLUORESCENCE SPECTRA OF THE FLAVO-COMPONENTS.63
OPTICAL ABSORPTION SPECTRA OF NONADOXIN REDUCTASE AND ENZYME-FREE FLAVIN
FLUORESCENCE SPECTRA OF NONADOXIN REDUCTASE AND ENZYME-FREE FLAVIN
33.2.3 IDENTIFICATION OF THE PROSTHETIC GROUP OF NONADOXIN REDUCTASE.
64
3.3.3 EPILOGUE ON THE PHYSICO-CHEMICAL PROPERTIES OF THE
P450NON
SYSTEM.
64
3.4
MOLECULAR RECOGNITION BETWEEN THE COMPONENTS OF THE
P450NON
SYSTEM.
65
3.4.1 INTERACTION BETWEEN NONADOXIN AND NONADOXIN REDUCTASE.65
3.4.1.1 IMPORTANCE OF THE ELECTROSTATIC FORCES IN INTERACTION.65
3.4.1.2 REVELATION OF A BINARY COMPLEX FORMATION BETWEEN NONADOXIN AND
NONADOXIN
REDUCTASE AND THE ROLE OF ELECTROSTATIC FORCES.67
3.4.1.3 REAL-TIME MONITORING OF THE INTERACTION BETWEEN NONADOXIN AND
NONADOXIN
REDUCTASE BY OPTICAL BIOSENSORS.67
3.4.2
INTERACTION BETWEEN
P450NON
AND NONADOXIN.6?
3.4.2.1
DISAGGREGATION OF
P450NON
MULTIMERS BY NONADOXIN.69
3.4.2.2
EPR
CHANGES IN NONADOXIN UPON ITS BINDING WITH
P450NON
.70
3.4.2.3
REVELATION OF A BINARY COMPLEX BETWEEN
P450NON
AND NONADOXIN, AND THE ROLE OF
ELECTROSTATIC FORCES IN ITS FORMATION.7.1
3.4.2.4
REAL-TIME MONITORING OF THE INTERACTION BETWEEN
P450NON
AND NONADOXIN BY
OPTICAL BIOSENSORS.7?
3.4.3
FURTHER INTERACTIONS BETWEEN THE COMPONENTS OF THE
P450NON
SYSTEM.7.?.
3.4.3.1
INTERACTION BETWEEN
P450NON
AND NONADOXIN REDUCTASE.7?.
3.4.3.2 COMPLEX FORMATION IN INDIVIDUAL PROTEIN PAIRS. .74
3.4.4 REVELATION OF A PUTATIVE TERNARY COMPLEX IN
P450NON
SYSTEM.75
3.5
ENZYMATIC ACTIVITY OF THE PURIFIED
P450NON
SYSTEM.77
3.5.1
SUBSTRATES OF
P450NON,
AS DETERMINED BY
NADH
OXIDATION.77
3.5.2 OXIDATION OF -[L
14C]-HEXADECANE AND [L-L4C]-PALMITIC ACID BY P450NON SYSTEM 78
3.5.2.1
EFFECT OF IONIC STRENGTH, PH AND TEMPERATURE ON THE ACTIVITY OF THE
P450NON
SYSTEM.$0
3.5.2.2
AMPHIPHILES ND THE FUNCTIONAL ACTIVITY OF THE
P450NON
SYSTEM.
81
3.5.2.3 EFFICACY OF '.TIE RECOMBINANT NONADOXIN AND NONADOXIN REDUCTASE
WITH P450
N N 84
3.5.2.4
EFFECT OF FOREIGN FERREDOXI NS ON THE EFFICIENCY OF
P450NON
.
84.
3.5.3
PROPOSAL FOR AN OPTIMAL MONOOXYGENASE ACTIVITY OF THE PURIFIED
P450NON
SYSTEM86
4 DISCUSSION.8.7
4.1 ESTABLISHMENT OF A PROTOCOL FOR THE PRODUCTION OF THE P450NON
SYSTEM.87
4.1.1 INDUCTION OF THE P450NAN SYSTEM AND PREPARATION OF THE CELL-FREE
EXTRACTS.87
4.1.2 PURIFICATION OF P450
RA N, NONADOXIN AND NONADOXIN REDUCTASE.89
4.2 MOLECULAR PROPERTIES OF P450NON, NONADOXIN AND NONADOXIN
REDUCTASE.92
4.2.1 PECULIAR FEATURES OF P450NON.
.92
P450NON AND ITS AGGREGATION
P450NON AND ITS SPIN STATE
POTENTIAL ASSOCIATION OF P450NON WITH MEMBRANE
4.2.2 MOLECULAR FEATURES OF NONADOXIN AND NONADOXIN REDUCTASE.9?
NONADOXIN AND ITS [FE-S] CLUSTER CONFIGURATION
NONADOXIN REDUCTASE VERSUS OTHER FERREDOXIN REDUCTASES
4.3 MOLECULAR RECOGNITION IN P450NON SYSTEM.
1.9.!.
4.3.1 OCCURRENCE OF VARIOUS BINARY COMPLEXES IN P450NON SYSTEM AND THEIR
NATURE.101
4.3.2 MODE OF INTERACTIONS IN P450NON SYSTEM.1.Q3
DYNAMIC OR SEQUENTIAL SHUTTLE MODEL
PUTATIVE TERNARY/QUATERNARY COMPLEX MODEL
4.4 CELL-FREE ACTIVITY RECONSTITUTION OF THE P450NON SYSTEM.;.105
4.4.1 CONDITIONS FOR THE OPTIMAL FUNCTION OF THE P450NON SYSTEM.105
INFLUENCE OF TEMPERATURE AND STABILITY OF THE COMPONENTS
CROSS REACTIVITY OF P450
YY
,N WITH FOREIGN FERREDOXINS
IONIC STRENGTH AND FUNCTIONAL RECONSTITUTION OF P450YYON
HOW AMPHIPHILIC MILIEU CAN MODULATE THE ACTIVITY OF P450NON?
DETERGENT-INDUCED SPIN CHANGE IN P450NON AND ITS ACTIVITY
4.4.2 SUBSTRATE SPECIFICITY AND REGIO-SELECTIVITY OF P450NON.!!.?
4.4.3 POTENTIAL
IN VR J MODEL FOR THE P450 NON SYSTEM.113
4.5 FUTURE FRON
.3 OF THE P450NON SYSTEM.114
5 BIBLIOGRAPHY.115 |
| any_adam_object | 1 |
| author | Gupta, Nishith |
| author_facet | Gupta, Nishith |
| author_role | aut |
| author_sort | Gupta, Nishith |
| author_variant | n g ng |
| building | Verbundindex |
| bvnumber | BV017294798 |
| ctrlnum | (OCoLC)633079697 (DE-599)BVBBV017294798 |
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| publishDateSort | 2003 |
| record_format | marc |
| spelling | Gupta, Nishith Verfasser aut P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons by Nishith Gupta 2003 123, 6 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Leipzig, Univ., Diss., 2003 Enzymatische Oxidation (DE-588)4468528-2 gnd rswk-swf Alkan-Monooxygenase Alkan-1-Monooxygenase (DE-588)4738811-0 gnd rswk-swf Acinetobacter (DE-588)4278742-7 gnd rswk-swf Alkane (DE-588)4191827-7 gnd rswk-swf Cytochrom P-450 (DE-588)4148705-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Acinetobacter (DE-588)4278742-7 s Cytochrom P-450 (DE-588)4148705-9 s Alkan-Monooxygenase Alkan-1-Monooxygenase (DE-588)4738811-0 s DE-604 Alkane (DE-588)4191827-7 s Enzymatische Oxidation (DE-588)4468528-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010426412&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gupta, Nishith P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons Enzymatische Oxidation (DE-588)4468528-2 gnd Alkan-Monooxygenase Alkan-1-Monooxygenase (DE-588)4738811-0 gnd Acinetobacter (DE-588)4278742-7 gnd Alkane (DE-588)4191827-7 gnd Cytochrom P-450 (DE-588)4148705-9 gnd |
| subject_GND | (DE-588)4468528-2 (DE-588)4738811-0 (DE-588)4278742-7 (DE-588)4191827-7 (DE-588)4148705-9 (DE-588)4113937-9 |
| title | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons |
| title_auth | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons |
| title_exact_search | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons |
| title_full | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons by Nishith Gupta |
| title_fullStr | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons by Nishith Gupta |
| title_full_unstemmed | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 in vitro model for the enzymatic oxidation of hydrocarbons by Nishith Gupta |
| title_short | P450-dependent alkane monooxygenase from Acinetobacter sp. EB104 |
| title_sort | p450 dependent alkane monooxygenase from acinetobacter sp eb104 in vitro model for the enzymatic oxidation of hydrocarbons |
| title_sub | in vitro model for the enzymatic oxidation of hydrocarbons |
| topic | Enzymatische Oxidation (DE-588)4468528-2 gnd Alkan-Monooxygenase Alkan-1-Monooxygenase (DE-588)4738811-0 gnd Acinetobacter (DE-588)4278742-7 gnd Alkane (DE-588)4191827-7 gnd Cytochrom P-450 (DE-588)4148705-9 gnd |
| topic_facet | Enzymatische Oxidation Alkan-Monooxygenase Alkan-1-Monooxygenase Acinetobacter Alkane Cytochrom P-450 Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010426412&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT guptanishith p450dependentalkanemonooxygenasefromacinetobacterspeb104invitromodelfortheenzymaticoxidationofhydrocarbons |