Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2003 |
| Beschreibung: | 120 Bl. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV017247698 | ||
| 003 | DE-604 | ||
| 005 | 20031119 | ||
| 007 | t| | ||
| 008 | 030626s2003 xx ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 967819547 |2 DE-101 | |
| 035 | |a (OCoLC)643357095 | ||
| 035 | |a (DE-599)BVBBV017247698 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91 |a DE-12 | ||
| 084 | |a CHE 820d |2 stub | ||
| 100 | 1 | |a Ksiazek, Dorota |d 1974- |e Verfasser |0 (DE-588)124734936 |4 aut | |
| 245 | 1 | 0 | |a Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |c Dorota Ksiazek |
| 264 | 1 | |c 2003 | |
| 300 | |a 120 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a München, Techn. Univ., Diss., 2003 | ||
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Grün fluoreszierendes Protein |0 (DE-588)4508128-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Adenylatcyclase |0 (DE-588)4141414-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Dictyostelium discoideum |0 (DE-588)4012135-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Strukturaufklärung |0 (DE-588)4183788-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Grün fluoreszierendes Protein |0 (DE-588)4508128-1 |D s |
| 689 | 0 | 1 | |a Strukturaufklärung |0 (DE-588)4183788-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Dictyostelium discoideum |0 (DE-588)4012135-5 |D s |
| 689 | 1 | 1 | |a Adenylatcyclase |0 (DE-588)4141414-7 |D s |
| 689 | 1 | 2 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 1 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010397102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010397102 | |
Datensatz im Suchindex
| _version_ | 1816445112451858432 |
|---|---|
| adam_text |
CONTENTS
CONTENTS
1. INTRODUCTION 1
2. METHODS FOR STRUCTURAL STUDIES 3
2.1 X-RAY CRYSTALLOGRAPHY 3
2.1.1 GENERAL BACKGROUND 3
2.1.2 WHAT IS A PROTEIN CRYSTAL 4
2.1.3 CRYSTAL GROWTH 4
2.1.4 CRYSTALLINE LATTICE 6
2.1.5 X-RAY DIFFRACTION BY CRYSTALS 7
2.1.6 THE PHASE PROBLEM 8
2.2 NMR SPECTROSCOPY 14
2.2.1 GENERAL BACKGROUND 14
2.2.2 ONE-DIMENSIONAL NMR 15
2.2.3 TWO-DIMENSIONAL NMR 18
3. MATERIALS AND LABORATORY METHODS 21
3.1 MATERIALS 21
3.2 MOLECULAR BIOLOGY TECHNIQUES 29
3.3 TOOLS OF BIOCHEMISTRY 31
4. PRELIMINARY INVESTIGATIONS ON THE FLUORESCENT PROTEIN FAMILY 36
4.1 'H,
I5N, 13C NMR SPECTROSCOPY OF GFPUV 36
4.1.1 BIOLOGICAL BACKGROUND 36
4.1.2 SAMPLE PREPARATION 3 7
4.1.3 OPTICAL SPECTROSCOPY 38
4.1.4 NMR SPECTROSCOPY 38
4.1.5 NMR DIFFUSION MEASUREMENTS 39
4.1.6 SEQUENCE ALIGNMENT 40
4.1.7 TRANSLATIONAL DIFFUSION 42
4.1.8 NMR ASSIGNMENT 44
4.1.9 MUTANT HIS 148GLY 44
4.1.10 DISCUSSION 47
4.2
19F NMR SPECTROSCOPY OF EGFP, ECFP AND EYFP 53
4.2.1 BIOLOGICAL BACKGROUND 53
4.2.2 PROTEIN EXPRESSION AND PURIFICATION 54
4.2.3 OPTICAL SPECTROSCOPY 55
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/967819547
CONTENTS
4.2.4 NMR SPECTROSCOPY 55
4.2.5 MODELLING OF THE CFP STRUCTURE 56
4.2.6 UV AND FLUORESCENCE SPECTROSCOPY 57
4.2.7 NMR ASSIGNMENT 59
4.2.8 THERMODYNAMIC ANALYSIS 61
4.2.9 INFLUENCE OF DENATURATION, PH, PROTEIN CONCENTRATION AND
IRRADIATION WITH UV LIGHT 61
4.2.10 DISCUSSION 63
4.3 CRYSTAL STRUCTURE OF DSRED 69
4.3.1 BIOLOGICAL BACKGROUND 69
4.3.2 PROTEIN EXPRESSION AND PURIFICATION 70
4.3.3 CRYSTALLIZATION TRIALS 71
4.3.4 STRUCTURE DETERMINATION 71
4.3.5 DESCRIPTION OF THE STRUCTURE 73
4.3.6 OLIGOMERIZATION 75
4.3.7 THE DSRED CHROMOPHORE STRUCTURE AND FEATURES PERSPECTIVES 76
4.4 CONCLUSIONS 78
5. STRUCTURE OF THE N-TERMINAL DOMAIN OF THE ADENYLYL CYCLASE-ASSOCIATED
PROTEIN
(CAP) FROM
DICTYOSTELIUM DISCOIDEUM 80
5.1 BIOLOGICAL BACKGROUND 80
5.2 CLONING, EXPRESSION AND CRYSTALLIZATION OF N-TERMINAL CAP 81
5.3 STRUCTURE DETERMINATION AND REFINEMENT 83
5.4 N-TERMINAL DOMAIN CONSTRUCTS OF THE CAP 86
5.5 STRUCTURE DESCRIPTION 87
5.6 OVERALL FOLD 89
5.7 STRUCTURAL COMPARISONS 91
5.8 CAP IN
DICTYOSTELIUM IS A MULTIMER 93
5.9 DISCUSSION 94
5.10 BIOLOGICAL IMPLICATIONS 96
6. SUMMARY 97
7. ZUSAMMENFASSUNG 99
8. REFERENCES 191
9. APPENDIX: ABBREVIATIONS AND SYMBOLS 118 |
| any_adam_object | 1 |
| author | Ksiazek, Dorota 1974- |
| author_GND | (DE-588)124734936 |
| author_facet | Ksiazek, Dorota 1974- |
| author_role | aut |
| author_sort | Ksiazek, Dorota 1974- |
| author_variant | d k dk |
| building | Verbundindex |
| bvnumber | BV017247698 |
| classification_tum | CHE 820d |
| ctrlnum | (OCoLC)643357095 (DE-599)BVBBV017247698 |
| discipline | Chemie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV017247698</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20031119</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">030626s2003 xx ad|| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">967819547</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)643357095</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV017247698</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 820d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ksiazek, Dorota</subfield><subfield code="d">1974-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)124734936</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein</subfield><subfield code="c">Dorota Ksiazek</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">120 Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">München, Techn. Univ., Diss., 2003</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Grün fluoreszierendes Protein</subfield><subfield code="0">(DE-588)4508128-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Adenylatcyclase</subfield><subfield code="0">(DE-588)4141414-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Dictyostelium discoideum</subfield><subfield code="0">(DE-588)4012135-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Strukturaufklärung</subfield><subfield code="0">(DE-588)4183788-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Grün fluoreszierendes Protein</subfield><subfield code="0">(DE-588)4508128-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Strukturaufklärung</subfield><subfield code="0">(DE-588)4183788-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Dictyostelium discoideum</subfield><subfield code="0">(DE-588)4012135-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Adenylatcyclase</subfield><subfield code="0">(DE-588)4141414-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010397102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-010397102</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017247698 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:37:30Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010397102 |
| oclc_num | 643357095 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-12 |
| owner_facet | DE-91 DE-BY-TUM DE-12 |
| physical | 120 Bl. Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| record_format | marc |
| spelling | Ksiazek, Dorota 1974- Verfasser (DE-588)124734936 aut Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein Dorota Ksiazek 2003 120 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2003 Proteine (DE-588)4076388-2 gnd rswk-swf Grün fluoreszierendes Protein (DE-588)4508128-1 gnd rswk-swf Adenylatcyclase (DE-588)4141414-7 gnd rswk-swf Dictyostelium discoideum (DE-588)4012135-5 gnd rswk-swf Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Grün fluoreszierendes Protein (DE-588)4508128-1 s Strukturaufklärung (DE-588)4183788-5 s DE-604 Dictyostelium discoideum (DE-588)4012135-5 s Adenylatcyclase (DE-588)4141414-7 s Proteine (DE-588)4076388-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010397102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ksiazek, Dorota 1974- Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein Proteine (DE-588)4076388-2 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd Adenylatcyclase (DE-588)4141414-7 gnd Dictyostelium discoideum (DE-588)4012135-5 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4508128-1 (DE-588)4141414-7 (DE-588)4012135-5 (DE-588)4183788-5 (DE-588)4113937-9 |
| title | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |
| title_auth | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |
| title_exact_search | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |
| title_full | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein Dorota Ksiazek |
| title_fullStr | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein Dorota Ksiazek |
| title_full_unstemmed | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein Dorota Ksiazek |
| title_short | Structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |
| title_sort | structural investigations on green fluorescent protein variants and the adenylyl cyclase associated protein |
| topic | Proteine (DE-588)4076388-2 gnd Grün fluoreszierendes Protein (DE-588)4508128-1 gnd Adenylatcyclase (DE-588)4141414-7 gnd Dictyostelium discoideum (DE-588)4012135-5 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| topic_facet | Proteine Grün fluoreszierendes Protein Adenylatcyclase Dictyostelium discoideum Strukturaufklärung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010397102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ksiazekdorota structuralinvestigationsongreenfluorescentproteinvariantsandtheadenylylcyclaseassociatedprotein |