Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Cramer
2003
|
| Schriftenreihe: | Bibliotheca mycologica
197 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 139 S. Ill., graph. Darst. |
| ISBN: | 3443590993 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
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| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
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| 100 | 1 | |a Abang, Mathew Musumbale |d 1970- |e Verfasser |0 (DE-588)124948812 |4 aut | |
| 245 | 1 | 0 | |a Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria |c Mathew Musumbale Abang |
| 264 | 1 | |a Berlin [u.a.] |b Cramer |c 2003 | |
| 300 | |a XI, 139 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Bibliotheca mycologica |v 197 | |
| 502 | |a Zugl.: Göttingen, Univ., Diss., 2002 | ||
| 650 | 4 | |a Colletotrichum gloeosporioides |z Nigeria |x Genetics | |
| 650 | 4 | |a Yam anthracnose | |
| 650 | 0 | 7 | |a Genetische Variabilität |0 (DE-588)4264352-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Anthraknose |0 (DE-588)4142635-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Jamswurzel |0 (DE-588)4162700-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Glomerella cingulata |0 (DE-588)4283882-4 |2 gnd |9 rswk-swf |
| 651 | 7 | |a Nigeria |0 (DE-588)4042300-1 |2 gnd |9 rswk-swf | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Nigeria |0 (DE-588)4042300-1 |D g |
| 689 | 0 | 1 | |a Jamswurzel |0 (DE-588)4162700-3 |D s |
| 689 | 0 | 2 | |a Anthraknose |0 (DE-588)4142635-6 |D s |
| 689 | 0 | 3 | |a Glomerella cingulata |0 (DE-588)4283882-4 |D s |
| 689 | 0 | 4 | |a Genetische Variabilität |0 (DE-588)4264352-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 830 | 0 | |a Bibliotheca mycologica |v 197 |w (DE-604)BV035361777 |9 197 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010387662&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010387662 | |
Datensatz im Suchindex
| _version_ | 1817711149860257792 |
|---|---|
| adam_text |
V
CONTENTS
ABBREVIATIONS
VIII
LIST
OF
FIGURES
X
LIST
OF
TABLES
XI
1
INTRODUCTION
.
1
1.1
ORIGIN
AND
IMPORTANCE
OF
YAM
.
1
1.2
CONSTRAINTS
TO
YAM
PRODUCTION
.
2
1.3
YAM
ANTHRACNOSE
DISEASE
EPIDEMIOLOGY
.
3
1.4
C.
GLOEOSPORIOIDES
PHYTOTOXINS
AND
ANTHRACNOSE
SYMPTOMS
.
4
1.5
OPTIONS
FOR
THE
CONTROL
OF
YAM
ANTHRACNOSE
.
5
1.6
IDENTIFICATION
OF
C.
GLOEOSPORIOIDES
.
6
1.7
GENETIC
DIVERSITY
OF
C.
GLOEOSPORIOIDES
FROM
YAM
.
8
1.7.1
CONVENTIONAL
METHODS
.
8
1.7.2
MOLECULAR-GENETIC
APPROACHES
.
9
1.8
OBJ
ECTIVES
OF
THE
STUDY
.
11
2
MATERIALS
AND
METHODS
.
13
2.1
FIELD
SURVEY
AND
SAMPLE
COLLECTION
.
13
2.2
ISOLATION,
MAINTENANCE
AND
STORAGE
OF
FUNGAL
ISOLATES
.
14
2.2.1
ISOLATION
OF
C.
GLOEOSPORIOIDES
.
14
2.2.2
ESTABLISHMENT
OF
SINGLE
SPORE
CULTURES
.
14
2.2.3
MAINTENANCE
AND
STORAGE
OF
CULTURES
.
15
2.3
MORPHOLOGICAL,
CULTURAL
AND
BIOCHEMICAL
CHARACTERIZATION
.
15
2.3.1
MORPHOLOGICAL
AND
CULTURAL
CHARACTERIZATION
.
15
2.3.2
BIOCHEMICAL
CHARACTERIZATION
OF
C.
GLOEOSPORIOIDES
MORPHOTYPES
.
17
2.3.3
ANALYSIS
OF
MORPHOLOGICAL,
CULTURAL
AND
BIOCHEMICAL
DATA
.
17
2.4
FUNGICIDE
SENSITIVITY
ASSAYS
.
18
2.5
VIRULENCE
ANALYSIS
AND
CROSS-INOCULATION
TESTS
.
18
2.5.1
VIRULENCE
PHENOTYPING
ON
DIOSCOREA
ALATA
.
18
2.5.2
CROSS-INOCULATION
TESTS
.
19
VI
2.6
VEGETATIVE
COMPATIBILITY
TESTS
.
20
2.6.1
GENERATION
OF
NIT
MUTANTS
.
20
2.6.2
CHARACTERIZATION
OF
NIT
PHENOTYPES
.
20
2.6.3
COMPLEMENTATION
TESTS
AND
ASSIGNMENT
OF
ISOLATES
TO
VCGS
.
21
2.7
NUCLEIC
ACID
ANALYSIS
.
21
2.7.1
DNA
EXTRACTION
.
21
2.7.2
DETERMINATION
OF
DNA
CONCENTRATION
AND
GEL
ELECTROPHORESIS
.
22
2.7.3
AMPLIFICATION
OF
THE
ITS
REGION
.
23
2.7.4
RESTRICTION
ENZYME
ANALYSIS
OF
RDNA
ITS
AMPLICONS
.
24
2.7.5
PURIFICATION
AND
CLONING
OF
ITS
AMPLICONS
.
24
2.7.6
TRANSFORMATION
OF
ESCHERICHIA
COLI
.
25
2.7.7
SEQUENCE
ANALYSIS
OF
ITS
RDNA
.
28
2.8
GENETIC
ANALYSIS
OF
C.
GLOEOSPORIOIDES
POPULATIONS
.
30
2.8.1
STRATEGIES
FOR
SAMPLING
GENETIC
DIVERSITY
IN
C.
GLOEOSPORIOIDES
.
30
2.8.2
MICROSATELLITE-PRIMED
PCR
ANALYSIS
.
31
2.8.3
GENETIC
ANALYSES
.
32
2.9
CHARACTERIZATION
OF
C.
GLOEOSPORIOIDES
PHYTOTOXIN
.
34
2.9.1
FUNGAL
CULTURES,
TOXIN
EXTRACTION
AND
PURIFICATION
.
34
2.9.2
CHEMICAL
ANALYSIS
OF
C.
GLOEOSPORIOIDES
PHYTOTOXIN
.
35
2.9.3
BIOLOGICAL
ASSAYS
.
37
2.9.4
ANTHRACNOSE
RESISTANCE
SCREENING
OF
YAM
TISSUE
CULTURES
USING
C.
GLOEOSPORIOIDES
PHYTOTOXIN
.
37
3
RESULTS
.
41
3.1
SURVEY
OF
C.
GLOEOSPORIOIDES
.
41
3.2
MORPHOLOGICAL,
CULTURAL
AND
BIOCHEMICAL
CHARACTERIZATION
.
41
3.3
FUNGICIDE
SENSITIVITY
TESTS
.
47
3.4
VIRULENCE
SPECTRUM
OF
C.
GLOEOSPORIOIDES
ISOLATES
.
47
3.5
CROSS-INFECTION
POTENTIAL
.
50
3.6
VEGETATIVE
COMPATIBILITY
.
51
3.7
ITS
ANALYSIS
.
55
3.7.1
PCR
AMPLIFICATION
AND
RFLP
ANALYSIS
OF
THE
ITS
RDNA
.
55
VII
3.7.2
SEQUENCE
ANALYSIS
OF
THE
ITS
REGIONS
.
58
3.8
GENETIC
STRUCTURE
OF
C.
GLOEOSPORIOIDES
POPULATIONS
.
60
3.9
CHARACTERIZATION
OF
PHYTOTOXINS
.
69
3.9.1
PARTIAL
PURIFICATION
OF
C.
GLOEOSPORIOIDES
CULTURE
FILTRATES
.
69
3.9.2
BIOCHEMICAL
AND
BIOLOGICAL
ASSAYS
.
71
4
DISCUSSION
.
79
5
CONCLUSION
.
91
6
SUMMARY
.
93
7
REFERENCES
.
97
8
APPENDIX
.
117
8.1
APPENDIX
1:
REAGENTS,
SOLUTIONS,
BUFFERS
AND
CULTURE
MEDIA
.
117
8.1.1
MORPHOLOGICAL,
CULTURAL,
BIOCHEMICAL
AND
TOXIN
CHARACTERIZATION
.
117
8.1.2
DNA
EXTRACTION
.
122
8.1.3
AGAROSE
GEL
ELECTROPHORESIS
.
123
8.1.4
CLONING
AND
SEQUENCING
.
124
8.2
APPENDIX
2:
C.
GLOEOSPORIOIDES
ISOLATES
USED
IN
THIS
STUDY
.
127 |
| any_adam_object | 1 |
| author | Abang, Mathew Musumbale 1970- |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| geographic | Nigeria (DE-588)4042300-1 gnd |
| geographic_facet | Nigeria |
| id | DE-604.BV017236914 |
| illustrated | Illustrated |
| indexdate | 2024-12-06T17:00:37Z |
| institution | BVB |
| isbn | 3443590993 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010387662 |
| oclc_num | 52712914 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-188 |
| physical | XI, 139 S. Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Cramer |
| record_format | marc |
| series | Bibliotheca mycologica |
| series2 | Bibliotheca mycologica |
| spelling | Abang, Mathew Musumbale 1970- Verfasser (DE-588)124948812 aut Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria Mathew Musumbale Abang Berlin [u.a.] Cramer 2003 XI, 139 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bibliotheca mycologica 197 Zugl.: Göttingen, Univ., Diss., 2002 Colletotrichum gloeosporioides Nigeria Genetics Yam anthracnose Genetische Variabilität (DE-588)4264352-1 gnd rswk-swf Anthraknose (DE-588)4142635-6 gnd rswk-swf Jamswurzel (DE-588)4162700-3 gnd rswk-swf Glomerella cingulata (DE-588)4283882-4 gnd rswk-swf Nigeria (DE-588)4042300-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Nigeria (DE-588)4042300-1 g Jamswurzel (DE-588)4162700-3 s Anthraknose (DE-588)4142635-6 s Glomerella cingulata (DE-588)4283882-4 s Genetische Variabilität (DE-588)4264352-1 s DE-604 Bibliotheca mycologica 197 (DE-604)BV035361777 197 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010387662&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Abang, Mathew Musumbale 1970- Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria Bibliotheca mycologica Colletotrichum gloeosporioides Nigeria Genetics Yam anthracnose Genetische Variabilität (DE-588)4264352-1 gnd Anthraknose (DE-588)4142635-6 gnd Jamswurzel (DE-588)4162700-3 gnd Glomerella cingulata (DE-588)4283882-4 gnd |
| subject_GND | (DE-588)4264352-1 (DE-588)4142635-6 (DE-588)4162700-3 (DE-588)4283882-4 (DE-588)4042300-1 (DE-588)4113937-9 |
| title | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria |
| title_auth | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria |
| title_exact_search | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria |
| title_full | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria Mathew Musumbale Abang |
| title_fullStr | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria Mathew Musumbale Abang |
| title_full_unstemmed | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria Mathew Musumbale Abang |
| title_short | Genetic diversity of Colletotrichum gloeosporioides Penz. causing anthracnose disease of Yam (Dioscorea spp.) in Nigeria |
| title_sort | genetic diversity of colletotrichum gloeosporioides penz causing anthracnose disease of yam dioscorea spp in nigeria |
| topic | Colletotrichum gloeosporioides Nigeria Genetics Yam anthracnose Genetische Variabilität (DE-588)4264352-1 gnd Anthraknose (DE-588)4142635-6 gnd Jamswurzel (DE-588)4162700-3 gnd Glomerella cingulata (DE-588)4283882-4 gnd |
| topic_facet | Colletotrichum gloeosporioides Nigeria Genetics Yam anthracnose Genetische Variabilität Anthraknose Jamswurzel Glomerella cingulata Nigeria Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010387662&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035361777 |
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