Generic and specific cell adhesion: investigations of a model system by micro-interferometry
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2003 |
| Beschreibung: | IV, 145 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1816445109634334720 |
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| adam_text |
CONTENTS
ZUSAMMENFASSUNG 1
INTRODUCTION 5
1 DUAL WAVELENGTH TECHNIQUE 9
1.1 INTRODUCTION, MOTIVATION. 9
1.2 RICM. 9
1.2.1 REFLECTION INTERFERENCE. 9
1.2.2 ANTIFLEX TECHNIQUE. 11
1.2.3 RECONSTRUCTION OF THE HEIGHT PROFILE. 12
1.2.4 RICM SETUP. 14
1.2.5 DUAL WAVELENGTH TECHNIQUE. 16
1.3 OUTLOOK.
20
2 FLUCTUATIONS OF MEMBRANES 22
2.1 INTRODUCTION.
22
2.2 THEORY.
24
2.2.1 GENERIC FORCES .
2$
2.2.2 TOTAL INTERACTION POTENTIAL.26
2.3 MATERIALS AND METHODS.30
2.3.1 GIANT VESICLES. 30
2.3.2 SUBSTRATE PREPARATION.30
2.3.3 RICM.31
2.3.4 EVALUATION.31
2.4 RESULTS.36
2.4.1 SINGLE-COMPONENT MEMBRANE.36
2.4.2 MULTI-COMPONENT MEMBRANE .38
2.4.3 CHANGE OF THE SPECIFIC WEIGHT.43
2.4.4 PASSIVATION OF THE SUBSTRATE.43
2.5 DISCUSSION.44
2.6 CONCLUSIONS.46
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/968527191
3 MODEL SYSTEM FOR CELL PLASMA MEMBRANES 48
3.1 INTRODUCTION.48
3.1.1 INTEGRINS.51
3.1.2 RGD PEPTIDES.52
3.1.3 OUTLINE . 53
3.2 THEORY OF DIFFUSION. 53
3.2.1 THE HYDRODYNAMIC MODEL.54
3.2.2 DIFFUSION IN SUPPORTED MEMBRANES.55
3.3 MATERIALS AND METHODS. 56
3.3.1 GLASS SUBSTRATES .57
3.3.2 SILANIZATION OF THE GLASS SUBSTRATES.57
3.3.3 CELLULOSE FILMS. 57
3.3.4 RECONSTITUTION OF INTEGRINS.58
3.3.5 SUPPORTED PLANAR BILAYERS.59
3.3.6 BLOCKING NON-SPECIFIC ADHESION. 60
3.3.7 RGD LIPID .60
3.3.8 FLUORESCENCE MICROSCOPY.60
3.3.9 FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING (FRAP).60
3.3.10 CRYO-ELECTRON MICROSCOPY.62
3.4 RESULTS.62
3.4.1 RECONSTITUTION OF A//B/3
3 INTEGRINS INTO LIPID BILAYERS.62
3.4.2 DETERMINATION OF INTEGRIN AND LIPID CONCENTRATIONS.63
3.4.3 FORMATION OF PLANAR MEMBRANES WITH INTEGRINS .64
3.4.4 TUNABLE MODEL SYSTEM.66
3.4.5 DETERMINATION OF THE LATERAL DIFFUSION CONSTANT DR.67
3.4.6 DETERMINATION OF THE FRICTIONAL COUPLING. 70
3.4.7 FUNCTIONALITY OF THE INTEGRINS . 72
3.5 CONCLUSIONS. 73
4 CELL ADHESION STUDIES 75
4.1 INTRODUCTION. 75
4.2 STATE OF KNOWLEDGE. 76
4.3 THEORY OF VESICLE ADHESION. 79
4.3.1 BENDING ELASTIC THEORY . 79
4.3.2 LOCK-AND-KEY FORCES.83
4.3.3 REPULSION BY REPELLERS AND LIGANDS.84
4.4 MATERIALS AND METHODS.86
4.4.1 GIANT VESICLES.86
4.4.2 ADHESION ASSAY. 86
4.5 RESULTS AND DISCUSSION.87
4.5.1 CONTROL EXPERIMENTS.87
^.5.2 FREE ADHESION ENERGY DETERMINATION FOR GIANT VESICLES . 88
II
4.5.3 SPECIFIC ADHESION ENERGY OF THE INTEGRIN-RGD BINDING
. 96
4.6 CONCLUSIONS ON THE ADHESION STUDIES.98
4.7 OUTLOOK: CONTROLLING THE ADHESION AREA.98
5 MAGNETIC TWEEZERS 101
5.1 INTRODUCTION.101
5.2 MAGNETIC TWEEZERS.104
5.2.1 SETUP .104
5.2.2 FORCE TRANSDUCERS.106
5.2.3 CHARACTERIZATION .107
5.2.4 CALIBRATION .107
5.3 MATERIALS AND METHODS.108
5.3.1 RGD-CONTAINING VESICLES ON INTEGRIN-EXHIBITING SUBSTRATES . . 109
5.3.2
SIALYL-LEWISX-CONTAINING VESICLES ON E-SELECTIN
SUBSTRATES . . 109
5.3.3 ATTACHMENT OF BEADS.110
5.3.4 LASER CONFOCAL SCANNING MICROSCOPY (LCSM).110
5.4 MAGNETIC TWEEZERS EXPERIMENTS.110
5.4.1 MEMBRANE TENSION.HI
5.4.2 CONTACT ANGLE.112
5.4.3 VERTICAL FORCE.112
5.4.4 UNBINDING EXPERIMENTS .114
5.5 CONCLUSIONS.122
5.6 OUTLOOK.123
APPENDIX 125
BIBLIOGRAPHY 129
DANKE
I43
CURRICULUM VITAE 144
LIST OF PUBLICATIONS 145
III |
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| building | Verbundindex |
| bvnumber | BV017227277 |
| ctrlnum | (OCoLC)643338720 (DE-599)BVBBV017227277 |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV017227277 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:37:27Z |
| institution | BVB |
| language | English |
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| physical | IV, 145 S. Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
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| spelling | Gönnenwein, Stefanie 1973- Verfasser (DE-588)124898866 aut Generic and specific cell adhesion investigations of a model system by micro-interferometry Stefanie Gönnenwein 2003 IV, 145 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2003 Interferenzmikroskopie (DE-588)4162004-5 gnd rswk-swf Interferometrie (DE-588)4027296-5 gnd rswk-swf Zelladhäsion (DE-588)4314136-5 gnd rswk-swf Modell (DE-588)4039798-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zelladhäsion (DE-588)4314136-5 s Modell (DE-588)4039798-1 s Interferometrie (DE-588)4027296-5 s DE-604 Interferenzmikroskopie (DE-588)4162004-5 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010383582&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gönnenwein, Stefanie 1973- Generic and specific cell adhesion investigations of a model system by micro-interferometry Interferenzmikroskopie (DE-588)4162004-5 gnd Interferometrie (DE-588)4027296-5 gnd Zelladhäsion (DE-588)4314136-5 gnd Modell (DE-588)4039798-1 gnd |
| subject_GND | (DE-588)4162004-5 (DE-588)4027296-5 (DE-588)4314136-5 (DE-588)4039798-1 (DE-588)4113937-9 |
| title | Generic and specific cell adhesion investigations of a model system by micro-interferometry |
| title_auth | Generic and specific cell adhesion investigations of a model system by micro-interferometry |
| title_exact_search | Generic and specific cell adhesion investigations of a model system by micro-interferometry |
| title_full | Generic and specific cell adhesion investigations of a model system by micro-interferometry Stefanie Gönnenwein |
| title_fullStr | Generic and specific cell adhesion investigations of a model system by micro-interferometry Stefanie Gönnenwein |
| title_full_unstemmed | Generic and specific cell adhesion investigations of a model system by micro-interferometry Stefanie Gönnenwein |
| title_short | Generic and specific cell adhesion |
| title_sort | generic and specific cell adhesion investigations of a model system by micro interferometry |
| title_sub | investigations of a model system by micro-interferometry |
| topic | Interferenzmikroskopie (DE-588)4162004-5 gnd Interferometrie (DE-588)4027296-5 gnd Zelladhäsion (DE-588)4314136-5 gnd Modell (DE-588)4039798-1 gnd |
| topic_facet | Interferenzmikroskopie Interferometrie Zelladhäsion Modell Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010383582&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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