The brain from 25.000 feet: high level explorations of brain complexity, perception, induction and vagueness
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Dordrecht [u.a.]
Kluwer
2003
|
| Schriftenreihe: | Synthese library
317 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 316 S. Ill., graph. Darst. |
| ISBN: | 1402011768 |
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| 100 | 1 | |a Changizi, Mark A. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The brain from 25.000 feet |b high level explorations of brain complexity, perception, induction and vagueness |c by Mark A. Changizi |
| 264 | 1 | |a Dordrecht [u.a.] |b Kluwer |c 2003 | |
| 300 | |a 316 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Synthese library |v 317 | |
| 650 | 4 | |a Brain | |
| 650 | 4 | |a Brain |x physiology | |
| 650 | 4 | |a Computational neuroscience | |
| 650 | 4 | |a Mental Processes |x physiology | |
| 650 | 4 | |a Models, Neurological | |
| 650 | 4 | |a Neuropsychology | |
| 650 | 4 | |a Neurosciences | |
| 650 | 4 | |a Perception |x physiology | |
| 650 | 0 | 7 | |a Physiologische Psychologie |0 (DE-588)4076126-5 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804130040241192960 |
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| adam_text | CONTENTS 1 EXCLUSIVE RIGHTS IN LIFE: BIOTECHNOLOGY, GENETIC
MANIPULATION, AND INTELLECTUAL PROPERTY RIGHTS E.R. GOLD 1.1
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 1 1.2 BIOTECHNOLOGICAL INNOVATION . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2.1 PHYSICAL
INNOVATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. 3 1.2.1.1 DNA AND PROTEIN MOLECULES . . . . . . . . . . . . . . . . .
. 3 1.2.1.2 CELLS . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . 3 1.2.1.3 WHOLE ORGANISMS . . . . . . . . . . . . .
. . . . . . . . . . . . . 4 1.2.2 INFORMATION AND OTHER INTANGIBLES . .
. . . . . . . . . . . . . . . . . . 4 1.2.2.1 DNA SEQUENCES AND CELLS .
. . . . . . . . . . . . . . . . . . . 4 1.2.2.2 PROCESSES USING
BIOLOGICAL MATTER . . . . . . . . . . . . . 5 1.2.2.3 BIOINFORMATICS . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.3 SUMMARY .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 6 1.3 INTRODUCTION TO INTELLECTUAL PROPERTY RIGHTS . . . . . . . .
. . . . . . . . . . 6 1.3.1 EXCLUSIVE RIGHTS VS. RIGHTS TO THINGS . . .
. . . . . . . . . . . . . . . 6 1.3.2 PROPERTY AND INTELLECTUAL PROPERTY
RIGHTS . . . . . . . . . . . . . . 7 1.3.3 TRADE SECRETS . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.3.3.1
SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
8 1.3.3.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 8 1.3.4 PATENTS . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . 8 1.3.4.1 SUBJECT MATTER . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . 9 1.3.4.1.1 INVENTION
VS. DISCOVERY . . . . . . . . . . . . . . 10 1.3.4.1.2 EXCLUSIONS . . .
. . . . . . . . . . . . . . . . . . . . . . 10 1.3.4.2 REQUIREMENTS . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.3.4.2.1
SUBSTANTIVE CRITERIA . . . . . . . . . . . . . . . . . 11 1.3.4.2.1.1
NOVELTY . . . . . . . . . . . . . . . . . . 12 1.3.4.2.1.2 INVENTIVE
STEP (NONOBVIOUSNESS) . . . . . . . . . . 12 1.3.4.2.1.3 INDUSTRIAL
APPLICATION (UTILITY) . . . . . . . . . . . . . . . . . . 12 1.3.4.2.2
PROCEDURAL CRITERION: DISCLOSURE . . . . . . . 13 1.3.4.3 REMEDIES . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 1.3.5
COPYRIGHT AND DATABASE PROTECTION . . . . . . . . . . . . . . . . . . .
14 1.3.5.1 SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . 15 1.3.5.2 REQUIREMENTS . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . 15 1.3.5.3 REMEDIES . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . 16 1.3.6 PLANT VARIETY PROTECTION
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 1.3.6.1
SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
16 1.3.6.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . 17 1.4 CHALLENGES . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . 17 1.4.1 INCENTIVE VS.
ACCESS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
18 1.4.1.1 JUSTIFICATION FOR PROPERTY RIGHTS . . . . . . . . . . . . . .
. 18 1.4.1.2 ECONOMIC REALITY . . . . . . . . . . . . . . . . . . . . .
. . . . . . 19 1.4.2 FAIRNESS TO PROVIDERS OF BIOLOGICAL MATTER . . . .
. . . . . . . . . . 20 1.4.2.1 RIGHTS TO BIOLOGICAL MATTER . . . . . . .
. . . . . . . . . . . . 20 1.5 CONCLUSION . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 REFERENCES
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . 21 2 AGROBACTERIUM RHIZOGENES -MEDIATED
TRANSFORMATION OF PLANTS W. VA N DEVELDE, M. KARIMI, G. DEN HERDER, M.
VAN MONTAGU, M. HOLSTERS, AND S. GOORMACHTIG 2.1 INTRODUCTION . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . 23 2.2 ASPECTS INFLUENCING A. RHIZOGENES TRANSFORMATION EFFICIENCY .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . 26 2.2.1 CHOICE OF A. RHIZOGENES STRAIN . . . . . . . .
. . . . . . . . . . . . . . . 26 2.2.2 CHOICE OF EXPLANT . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 28 2.2.3 PREPARATION
OF BACTERIAL INOCULUM AND INFECTION OF EXPLANTS . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . 29 2.2.4
CO-CULTIVATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . 29 2.3 ESTABLISHING THE TRANSFORMED NATURE OF HAIRY ROOTS
. . . . . . . . . . . 31 2.4 COTRANSFORMATION OF BINARY T-DNA . . . . .
. . . . . . . . . . . . . . . . . . . . 31 2.5 PROPAGATION OF HAIRY ROOT
LINES IN LIQUID CULTURES . . . . . . . . . . . . 33 2.5.1 THE CLONAL
STATUS OF HAIRY ROOTS . . . . . . . . . . . . . . . . . . . . . 33 2.5.2
STABILITY OF LONG-TERM HAIRY ROOT CULTURES . . . . . . . . . . . . . 34
2.6 REGENERATION OF PLANTS FROM HAIRY ROOTS . . . . . . . . . . . . . .
. . . . . . 34 2.7 THE MULTI-AUTO-TRANSFORMATION (MAT) VECTOR SYSTEM . .
. . . . . . . . 35 2.8 CONCLUSIONS . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 37 PROTOCOL 1:
PRODUCTION OF TRANSFORMED HAIRY ROOTS . . . . . . . . . . . . . . . . 37
PROTOCOL 2: PLANT REGENERATION FROM HAIRY ROOTS . . . . . . . . . . . .
. . . . . . 38 PROTOCOL 3: HAIRY ROOT LIQUID CULTURE . . . . . . . . . .
. . . . . . . . . . . . . . . . . 39 REFERENCES . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
39 3 TRANSFORMATION OF PETUNIA HYBRIDA BY THE AGROBACTERIUM SUSPENSION
DROP METHOD S.J. WYLIE, D. TJOKROKUSUMO, AND J.A . MCCOMB 3.1
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 45 3.2 TRANSFORMATION . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 3.3
ANALYSIS OF TRANSFORMANTS . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . 47 X CONTENTS 3.3.1 SCREENING PETUNIA SEEDLINGS FOR
HERBICIDE RESISTANCE . . . . . 47 3.3.2 TRANSMISSION OF BASTA RESISTANCE
PHENOTYPE TO T2 PROGENY . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 48 3.3.3 B -GLUCURONIDASE ASSAY . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . 48 3.3.4 DNA ANALYSIS . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
3.4 CONCLUSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 49 REFERENCES . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4
ONION, LEEK AND GARLIC TRANSFORMATION BY CO-CULTIVATION WITH
AGROBACTERIUM C.C. EADY 4.1 INTRODUCTION . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . 53 4.1.1 CURRENT
APPLICATIONS OF ALLIUM TRANSFORMATION TECHNOLOGY . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . 53 4.1.1.1
PHYSIOLOGICAL STUDIES . . . . . . . . . . . . . . . . . . . . . . . . 53
4.1.1.2 HERBICIDE RESISTANCE . . . . . . . . . . . . . . . . . . . . . .
. . 54 4.1.1.3 ANTIMICROBIAL RESISTANCE . . . . . . . . . . . . . . . .
. . . . . 54 4.1.1.4 INSECT RESISTANCE . . . . . . . . . . . . . . . . .
. . . . . . . . . . 55 4.2 ONION TRANSFORMATION PROTOCOLS . . . . . . .
. . . . . . . . . . . . . . . . . . . . 55 4.2.1 TRANSFORMATION USING
ANTIBIOTIC AND VISUAL SELECTION . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . 56 4.2.1.1 BACTERIAL STRAIN AND
PLASMIDS . . . . . . . . . . . . . . . . . 56 4.2.1.2 TRANSFORMATION
PROCEDURE (MODIFIED FROM EADY ET AL. 2000 . . . . . . . . . . . . . . .
56 4.2.2 TRANSFORMATION USING HERBICIDE SELECTION . . . . . . . . . . .
. . . 57 4.2.2.1 BACTERIAL STRAIN AND PLASMIDS . . . . . . . . . . . . .
. . . . 58 4.2.2.2 TRANSFORMATION PROCEDURE . . . . . . . . . . . . . .
. . . . . 58 4.2.3 EX-FLASKING AND GROWTH IN CONTAINMENT . . . . . . . .
. . . . . . . 58 4.2.4 TRANSGENE DETECTION . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . 59 4.2.5 TRANSGENE EXPRESSION AND
STABILITY . . . . . . . . . . . . . . . . . . . 60 4.2.5.1 VISUAL
REPORTER GENES . . . . . . . . . . . . . . . . . . . . . . . 60 4.2.5.2
EXPRESSION OF HERBICIDE RESISTANCE . . . . . . . . . . . . 61 4.2.5.3
ANTISENSE ALLIINASE GENE EXPRESSION . . . . . . . . . . . 61 4.3 LEEK
TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . 62 4.4 GARLIC TRANSFORMATION PROTOCOL . . . . . . . . .
. . . . . . . . . . . . . . . . . . . 63 4.4.1 BACTERIAL STRAIN AND
PLASMIDS . . . . . . . . . . . . . . . . . . . . . . . . 63 4.4.2
TRANSFORMATION PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . .
. . 63 4.5 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 64 REFERENCES . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
5 ELECTROPORATION TRANSFORMATION OF BARLEY F. GUEREL AND N. GOEZUEKIRMIZI
5.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 69 5.2 BACKGROUND OF ELECTROPORATION
PROCEDURES . . . . . . . . . . . . . . . . . . . 71 CONTENTS XI 5.2.1
PRE- AND POST-ELECTROPORATION PERIOD . . . . . . . . . . . . . . . . . .
71 5.2.2 ELECTRICAL VARIABLES . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . 73 5.3 CULTURE AND ELECTROPORATION OF BARLEY
EXPLANTS . . . . . . . . . . . . . . . 74 5.3.1 PROTOPLASTS . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
5.3.2 MICROSPORES . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . 76 5.3.3 INTACT TISSUES . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . 77 5.3.3.1 ANALYSIS AND
INHERITANCE OF TRANSGENES IN ELECTROPORATED TISSUES . . . . . . . . . .
. . . . . . . . . . 81 5.4 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . .
. . . . . . . . . . . . . . . . . . . 83 REFERENCES . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . 84 6 SORGHUM TRANSFORMATION Z. ZHAO AND D. TOMES 6.1 INTRODUCTION .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . 91 6.2 SORGHUM TRANSFORMATION PROCESS AND OPTIMIZATION . . .
. . . . . . . . 92 6.2.1 PLANT MATERIALS AND TRANSFORMATION SYSTEMS . .
. . . . . . . . . . 92 6.2.2 TRANSFORMATION VIA MICROPROJECTILE
BOMBARDMENT . . . . . . . 93 6.2.3 AGROBACTERIUM -MEDIATED
TRANSFORMATION . . . . . . . . . . . . . . . 94 6.3 ANALYSIS OF
TRANSGENIC PLANTS AND THE PROGENY . . . . . . . . . . . . . . . . 96
6.3.1 MOLECULAR ANALYSIS OF T0 PLANTS . . . . . . . . . . . . . . . . .
. . . . . 97 6.3.2 FOREIGN GENE EXPRESSION IN T0 PLANTS . . . . . . . .
. . . . . . . . . . 98 6.3.3 GENETIC AND MOLECULAR ANALYSIS OF THE
PROGENY . . . . . . . . . . 99 6.4 MARKER-FREE SORGHUM TRANSGENIC PLANTS
. . . . . . . . . . . . . . . . . . . . . 99 6.4.1 IMPORTANCE OF
MARKER-FREE TRANSGENICS IN SORGHUM . . . . . . 100 6.4.2 METHODS TO
ELIMINATE MARKERS FROM TRANSGENIC PLANTS . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 100 6.4.3 AGROBACTERIUM 2 T-DNA
CO-TRANSFORMATION SYSTEM . . . . . . 101 REFERENCES . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . 102 7 TRANSGENIC SUNFLOWER: PEG-MEDIATED GENE TRANSFER P.C. BINSFELD
7.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 109 7.2 GENETIC VARIABILITY AND TRANSGENIC
BREEDING . . . . . . . . . . . . . . . . . . 109 7.3 GENE TRANSFER
SYSTEMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 110 7.3.1 PEG-MEDIATED GENE TRANSFER . . . . . . . . . . . . . . .
. . . . . . . . . 111 7.3.1.1 SHORT DNA MOLECULE UPTAKE . . . . . . . .
. . . . . . . . . . 111 7.3.1.2 LARGE DNA MOLECULE UPTAKE . . . . . . .
. . . . . . . . . . . 112 7.4 PLANT REGENERATION . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . 114 7.5 GENERAL
ANALYTICAL CONSIDERATIONS . . . . . . . . . . . . . . . . . . . . . . .
. . . 115 7.5.1 MOLECULAR ANALYSIS . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 115 7.5.1.1 DNA EXTRACTION . . . . . . . . . .
. . . . . . . . . . . . . . . . . . 116 7.5.1.2 SOUTHERN HYBRIDIZATION .
. . . . . . . . . . . . . . . . . . . . . 116 7.5.1.3 POLYMERASE CHAIN
REACTION . . . . . . . . . . . . . . . . . . 116 7.5.1.4 RANDOM
AMPLIFIED POLYMORPHIC DNA . . . . . . . . . . 117 XII CONTENTS 7.5.2
BIOCHEMICAL ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 118 7.5.2.1 MULTIPLE MOLECULAR FORMS OF ENZYMES . . . . . . .
. . . 118 7.5.2.2 ENZYMATIC ASSAY . . . . . . . . . . . . . . . . . . .
. . . . . . . . 119 7.5.3 CYTOGENETIC ANALYSIS . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . 120 7.5.3.1 FLOW CYTOMETRIC ANALYSIS
. . . . . . . . . . . . . . . . . . . . 120 7.5.3.2 MITOTIC AND MEIOTIC
CELL ANALYSIS . . . . . . . . . . . . . . 122 7.5.3.3 IN SITU
HYBRIDIZATION . . . . . . . . . . . . . . . . . . . . . . . . 122 7.5.4
MORPHOLOGICAL ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 123 7.6 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . . . . .
. . . . . . . . . . . . . . 124 REFERENCES . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
8 TRANSFORMATION OF NORWAY SPRUCE ( PICEA ABIES ) BY PARTICLE
BOMBARDMENT D.H. CLAPHAM, H. HAEGGMAN, M. ELFSTRAND, T. ARONEN, AND S.
VON ARNOLD 8.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 127 8.2 TYPES OF PARTICLE
ACCELERATOR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
127 8.3 TRANSFORMATION OF EMBRYOGENIC CULTURES . . . . . . . . . . . . .
. . . . . . . 128 8.3.1 TRANSIENT EXPRESSION IN EMBRYOGENIC CULTURES . .
. . . . . . . . 128 8.3.2 PRODUCTION OF STABLY TRANSFORMED CELL CULTURES
AND TRANSGENIC PLANTS . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 129 8.3.3 STABILITY OF TRANSGENE EXPRESSION . . . . . . . . .
. . . . . . . . . . . . 131 8.3.4 TRENDS IN TRANSGENIC PLANT PRODUCTION
. . . . . . . . . . . . . . . . . 131 8.4 TRANSFORMATION OF POLLEN . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 8.4.1
THE REPRODUCTIVE BIOLOGY OF NORWAY SPRUCE . . . . . . . . . . . . 133
8.4.2 TRANSIENT EXPRESSION IN POLLEN . . . . . . . . . . . . . . . . . .
. . . . . 134 8.4.3 DEVELOPMENT OF CONTROLLED POLLINATION TECHNIQUES FOR
BOMBARDED POLLEN . . . . . . . . . . . . . . . . . . . . 135 8.5
APPLICATIONS OF TRANSGENIC NORWAY SPRUCE IN RESEARCH . . . . . . . . .
136 8.5.1 GENES REGULATING EMBRYOGENESIS . . . . . . . . . . . . . . . .
. . . . . 136 8.5.2 GENES WITH SIMILARITY TO DEFENSE GENES . . . . . . .
. . . . . . . . . 137 8.6 PROSPECTS FOR TRANSGENIC NORWAY SPRUCE IN
PRACTICAL FORESTRY . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . 139 REFERENCES . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 143 9 WHISKERS-MEDIATED TRANSFORMATION OF MAIZE J.F. PETOLINO,
M. WELTER, AND C. QIHUA CAI 9.1 INTRODUCTION . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 9.2
PREPARATION OF PURIFIED DNA FRAGMENTS . . . . . . . . . . . . . . . . .
. . . . 147 9.3 ESTABLISHMENT AND MAINTENANCE OF EMBRYOGENIC SUSPENSION
CULTURES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 150 9.4 DNA DELIVERY VIA WHISKERS . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 152 9.5 TRANSGENE COPY NUMBER ESTIMATION .
. . . . . . . . . . . . . . . . . . . . . . . 153 9.6 REGENERATION OF
TRANSGENIC PLANTS AND PROGENY . . . . . . . . . . . . . . . 157 CONTENTS
XIII XIV CONTENTS 9.7 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . .
. . . . . . . . . . . . . . . . . 157 REFERENCES . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. 158 10 GENETIC TRANSFORMATION OF SOYBEAN WITH BIOLISTICS D. SIMMONDS
10.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . 159 10.2 TISSUE CULTURE AND PLANT
REGENERATION . . . . . . . . . . . . . . . . . . . . . . 160 10.2.1
GENOTYPE SPECIFICITY . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 160 10.2.2 INITIATION AND REPETITIVE PROLIFERATION OF SOMATIC
EMBRYOGENIC CULTURES . . . . . . . . . . . . . . . . . . . . . 161
10.2.3 EMBRYO HISTODIFFERENTIATION AND MATURATION . . . . . . . . . . .
. 163 10.2.4 GERMINATION, CONVERSION AND PLANT FERTILITY . . . . . . . .
. . . . 163 10.3 TRANSFORMATION . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 164 10.3.1 GENE DELIVERY . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
10.3.2 TARGET TISSUE OPTIMIZATION . . . . . . . . . . . . . . . . . . .
. . . . . . . 165 10.3.3 SELECTION . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 165 10.3.4 TRANSGENIC PLANT
RECOVERY . . . . . . . . . . . . . . . . . . . . . . . . . . 166 10.4
CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . 167 10.5 PROTOCOL . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
10.5.1 INDUCTION AND MAINTENANCE OF PROLIFERATIVE EMBRYOGENIC CULTURES .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 10.5.2
TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . 168 10.5.3 SELECTION . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . 169 10.5.4 PLANT REGENERATION .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . 170 11 GENOTOXIC EFFECTS OF TUNGSTEN
MICROPARTICLES UNDER CONDITIONS OF BIOLISTIC TRANSFORMATION J. BUCHOWICZ
AND C. KRYSIAK 11.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . 175 11.2 BIOLOGICAL
SIGNIFICANCE OF TUNGSTEN . . . . . . . . . . . . . . . . . . . . . . . .
. 175 11.2.1 EARLY OBSERVATIONS ON BIOLOGICAL EFFECTS OF TUNGSTEN . . .
. . 175 11.2.2 CATALYTIC ACTIVITY OF SIMPLE TUNGSTEN COMPOUNDS . . . . .
. . 176 11.2.3 TUNGSTOENZYMES . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 176 11.2.4 TUNGSTEN*DNA INTERACTION . . . . .
. . . . . . . . . . . . . . . . . . . . . 177 11.3 TUNGSTEN
MICROPARTICLES IN BIOTECHNOLOGICAL APPLICATIONS . . . . . . . 178 11.3.1
BIOLISTIC TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 178 11.3.1.1 AN OVERVIEW . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . 178 11.3.1.2 TECHNICAL DETAILS . . . . . . . . . . .
. . . . . . . . . . . . . . . . 180 11.3.2 BIOLISTIC INOCULATION AND
RELATED APPLICATIONS OF TUNGSTEN PARTICLES . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . 181 11.4 ASSESSMENT OF TUNGSTEN-INDUCED
DNA LESIONS . . . . . . . . . . . . . . . . 182 11.4.1 ELECTROPHORETIC
ANALYSIS OF TUNGSTEN-DAMAGED PLASMID DNA . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 182 11.4.2 A MODIFIED TUNEL
METHOD FOR DETECTION OF CELLULAR DNA FRAGMENTATION . . . . . . . . . . .
. . . . . . . . . . . 184 11.5 POST-BOMBARDMENT INHIBITION OF SOMATIC
EMBRYOGENESIS . . . . . . . 186 11.6 CONCLUDING REMARKS . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 188 REFERENCES .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . 188 SUBJECT INDEX . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
CONTENTS XV
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T19:14:42Z |
| institution | BVB |
| isbn | 1402011768 |
| language | English |
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| spelling | Changizi, Mark A. Verfasser aut The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness by Mark A. Changizi Dordrecht [u.a.] Kluwer 2003 316 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Synthese library 317 Brain Brain physiology Computational neuroscience Mental Processes physiology Models, Neurological Neuropsychology Neurosciences Perception physiology Physiologische Psychologie (DE-588)4076126-5 gnd rswk-swf Hirnfunktion (DE-588)4159930-5 gnd rswk-swf Hirnfunktion (DE-588)4159930-5 s Physiologische Psychologie (DE-588)4076126-5 s DE-604 Synthese library 317 (DE-604)BV000005044 317 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010356354&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Changizi, Mark A. The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness Synthese library Brain Brain physiology Computational neuroscience Mental Processes physiology Models, Neurological Neuropsychology Neurosciences Perception physiology Physiologische Psychologie (DE-588)4076126-5 gnd Hirnfunktion (DE-588)4159930-5 gnd |
| subject_GND | (DE-588)4076126-5 (DE-588)4159930-5 |
| title | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness |
| title_auth | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness |
| title_exact_search | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness |
| title_full | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness by Mark A. Changizi |
| title_fullStr | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness by Mark A. Changizi |
| title_full_unstemmed | The brain from 25.000 feet high level explorations of brain complexity, perception, induction and vagueness by Mark A. Changizi |
| title_short | The brain from 25.000 feet |
| title_sort | the brain from 25 000 feet high level explorations of brain complexity perception induction and vagueness |
| title_sub | high level explorations of brain complexity, perception, induction and vagueness |
| topic | Brain Brain physiology Computational neuroscience Mental Processes physiology Models, Neurological Neuropsychology Neurosciences Perception physiology Physiologische Psychologie (DE-588)4076126-5 gnd Hirnfunktion (DE-588)4159930-5 gnd |
| topic_facet | Brain Brain physiology Computational neuroscience Mental Processes physiology Models, Neurological Neuropsychology Neurosciences Perception physiology Physiologische Psychologie Hirnfunktion |
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