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PCR primer: a laboratory manual

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Bibliographic Details
Format:	Book
Language:	English
Published:	Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press 2003
Edition:	2. ed.
Subjects:	Biologia molecular Expressão gênica Genética molecular Genética Mutagênese Reação em cadeia por polimerase Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction > Laboratory Manuals Polymerase chain reaction > Laboratory manuals Primer > Molekulargenetik Polymerase-Kettenreaktion Methode
Online Access:	Inhaltsverzeichnis
Item Description:	Includes bibliographical references and index
Physical Description:	XI, 520 S. Ill., graph. Darst.
ISBN:	0879696532 0879696540

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MARC


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245	1	0	\|a PCR primer \|b a laboratory manual \|c ed. by Carl W. Dieffenbach ...
250			\|a 2. ed.
264		1	\|a Cold Spring Harbor, NY \|b Cold Spring Harbor Laboratory Press \|c 2003
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Record in the Search Index

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adam_text	PCR PRIMER A L A B O R A T O R Y M A N U A L SECOND EDITION TECHNISCHE UNIVERSITAT DARMSTADT FACHBEREICH 10 * BIOLOGIE * BIBLIOTHEK * SCHNITTSPAHNSTRAGE 10 0-64287 DARMSTADT FNV^NR. EDITED BY CARL W. DIEFFENBACH NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES GABRIELA S. DVEKSLER UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES COLD SPRING HARBOR LABORATORY PRESS COLD SPRING HARBOR, NEW YORK CONTENTS PREFACE, XI 111 INTRODUCTION TO PCR, 1 1. SETTING UP A PCR LABORATORY, 5 THEODORE E. MIFFLIN 2. DEALING WITH CARRYOVER CONTAMINATION IN PCR: AN ENZYMATIC STRATEGY, 15 KYUSUNG PARK AND JUN LEE 3. HIGH-FIDELITY PCR ENZYMES, 21 HOLLY H. HOGREFE AND MICHAEL C. BOMS 4. OPTIMIZATION AND TROUBLESHOOTING IN PCR, 35 KENNETH H. ROUX 5. PCR AMPLIFICATION OF HIGHLY GC-RICH REGIONS, 43 LISE LOTTE HANSEN AND JUST JUSTESEN 6. TIPS FOR LONG AND ACCURATE PCR, 53 WAYNE M. BARNES 7. PCR PRIMER DESIGN, 61 ARUN APTE AND SAURABHA DANIEL 8. NONRADIOACTIVE CYCLE SEQUENCING OF PCR-AMPLIFIED DNA, 75 SUSAN R. HAYNES IS SAMPLE PREPARATION, 83 9. DNA PURIFICATION, 87 CRAIG SMITH, PAUL OTTO, REX BITNER, AND GARY SHIELS 10. RNA PURIFICATION, 117 MICHAEL A. CONNOLLY, PETER A. CLAUSEN, AND JAMES G. LAZAR VII VIII U CONTENTS 11. IN SITU LOCALIZATION OF GENE EXPRESSION USING LASER CAPTURE MICRODISSECTION, 135 MOTOKO MORIMOTO, MASAHIRO MORIMOTO, JEANNETTE WHITMIRE, ROBERT A. STAR, JOSEPH F. URBAN, JR., AND WILLIAM C. GAUSE 12. STRATEGIES FOR OVERCOMING PCR INHIBITION, 149 PETER RADSTROM, CHARLOTTA LBFSTROM, MARIA LOVENKLEV, RICKARD KNUTSSON, AND PETRA WOLFFS S RT-PCR METHODS, 163 13. THE USE OF INTERNAL CONTROLS FOR RELATIVE AND COMPETITIVE QUANTITATIVE RT-PCR, 167 RENEE M. HOMER 14. COMPARISON OF FOUR REAL-TIME PCR DETECTION SYSTEMS: BIO-RAD I-CYCLER, ABI 7700, ROCHE LIGHTCYCLER, AND THE CEPHEID SMARTCYCLER, 187 KATHERINE E. TEMPLETON AND ERIC C.J. CLAAS 15. NEW TECHNOLOGY FOR QUANTITATIVE PCR, 199 WOLFGANG KUSSER, SANDRINE JAVORSCHI, AND MARTIN A. GLEESON 16. AMPLIFICATION OF RNA: HIGH-TEMPERATURE REVERSE TRANSCRIPTION AND DNA AMPLIFICATION WITH A MAGNESIUM-ACTIVATED THERMOSTABLE DNA POLYMERASE, 211 EDWARD S. SMITH, ALVIN K. LI, ALICE M. WANG, DAVID H. GELFAND, AND THOMAS W. MYERS H DETECTION OF PCR PRODUCTS: SPECIALIZED APPLICATIONS, 221 17. LOSS OF HETEROZYGOSITY, A MULTIPLEX PCR METHOD TO DEFINE NARROW DELETED CHROMOSOMAL REGIONS OF A TUMOR GENOME, 223 LISE LOTTE HANSEN AND JUST JUSTESEN 18. SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS, 237 ANNE E. JEDLICKA AND STEVEN R. KLEEBERGER 19. REVERSE TRANSCRIPTASE IN SITU PCR, 251 GERARD J. NUOVO 20. SENSITIVE AND FAST MUTATION DETECTION BY SOLID-PHASE CHEMICAL CLEAVAGE OF MISMATCHES, 265 LISE LOTTE HANSEN, JUST JUSTESEN, ANDREANA LAMBRINAKOS, AND RICHARD G.H. COTTON EH ANALYSIS OF DIFFERENTIAL GENE EXPRESSION BY PCR, 279 21. APPLYING PCR FOR MICROARRAY-BASED GENE EXPRESSION ANALYSIS, 281 JOAN M. ZAKEL 22. IDENTIFICATION OF DIFFERENTIAL GENES BY SUPPRESSION SUBTRACTIVE HYBRIDIZATION, 297 DENIS V. REBRIKOV CONTENTS * IX 23. FLUORESCENT MRNA DIFFERENTIAL DISPLAY IN GENE EXPRESSION ANALYSIS, 327 JEFFREY S. FISHER, JONATHAN MEADE, JAMIE WALDEN, ZHEN GUO, AND PENG LIANG B CLONING BY PCR, 349 24. PCR-BASED METHOD FOR SCREENING LIBRARIES, 351 DAVID I. ISRAEL 25. BEYOND CLASSIC RACE (RAPID AMPLIFICATION OF CDNA ENDS), 359 MICHAEL A. FROHMAN 26. SYNTHESIS AND ANALYSIS OF DNA LIBRARIES USING PCR, 391 JOHN L. JOYAL, JINWEI JIANG, AND DAVID I. ISRAEL I * CLONING OF PCR PRODUCTS, 403 27. STRATEGIES FOR CLONING PCR PRODUCTS, 405 PETER D ARPA 28. BIDIRECTIONAL AND DIRECTIONAL CLONING OF PCR PRODUCTS, 421 GINA L. COSTA AND MICHAEL P. WEINER 29. RIBOCLONING: DNA CLONING AND GENE CONSTRUCTION USING PCR PRIMERS TERMINATED WITH A RIBONUCLEOTIDE, 441 WAYNE M. BARNES ._B_ MUTAGENESIS BY PCR, 451 30. MUTAGENIC PCR, 453 R. CRAIG CADWELL AND GERALD F. JOYCE 31. RAPID PCR SITE-DIRECTED MUTAGENESIS, 459 MICHAEL P. WEINER AND GINA L. COSTA 32. MUTAGENESIS AND SYNTHESIS OF NOVEL RECOMBINANT GENES USING PCR, 467 ABBE N. VALLEJO, ROBERT J. POGULIS, AND LARRY R. PEASE 33. STREAMLINED GENE ASSEMBLY PCR, 475 WAYNE M. BARNES AND ELAINE R. FRAWLEY APPENDICES 1. LICENSING, 483 2. MODIFICATION OF OLIGONUCLEOTIDES FOR USE IN PCR, 485 3. CAUTIONS, 491 4. SUPPLIERS, 503 INDEX, 505
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language	English
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physical	XI, 520 S. Ill., graph. Darst.
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spelling	PCR primer a laboratory manual ed. by Carl W. Dieffenbach ... 2. ed. Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press 2003 XI, 520 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s Primer Molekulargenetik (DE-588)4318719-5 s Methode (DE-588)4038971-6 s DE-604 Dieffenbach, Carl W. Sonstige oth HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010352011&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	PCR primer a laboratory manual Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd
subject_GND	(DE-588)4318719-5 (DE-588)4256726-9 (DE-588)4038971-6
title	PCR primer a laboratory manual
title_auth	PCR primer a laboratory manual
title_exact_search	PCR primer a laboratory manual
title_full	PCR primer a laboratory manual ed. by Carl W. Dieffenbach ...
title_fullStr	PCR primer a laboratory manual ed. by Carl W. Dieffenbach ...
title_full_unstemmed	PCR primer a laboratory manual ed. by Carl W. Dieffenbach ...
title_short	PCR primer
title_sort	pcr primer a laboratory manual
title_sub	a laboratory manual
topic	Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd
topic_facet	Biologia molecular Expressão gênica Genética molecular Genética Mutagênese Reação em cadeia por polimerase Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik Polymerase-Kettenreaktion Methode
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010352011&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT dieffenbachcarlw pcrprimeralaboratorymanual

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