PCR primer: a laboratory manual
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Cold Spring Harbor, NY
Cold Spring Harbor Laboratory Press
2003
|
| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 520 S. Ill., graph. Darst. |
| ISBN: | 0879696532 0879696540 |
Internformat
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| 245 | 1 | 0 | |a PCR primer |b a laboratory manual |c ed. by Carl W. Dieffenbach ... |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Cold Spring Harbor, NY |b Cold Spring Harbor Laboratory Press |c 2003 | |
| 300 | |a XI, 520 S. |b Ill., graph. Darst. | ||
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| 500 | |a Includes bibliographical references and index | ||
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| 650 | 4 | |a Réaction en chaîne de la polymérase - Manuels de laboratoire | |
| 650 | 4 | |a Polymerase Chain Reaction |v Laboratory Manuals | |
| 650 | 4 | |a Polymerase chain reaction |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804130033995874304 |
|---|---|
| adam_text | PCR PRIMER A L A B O R A T O R Y M A N U A L SECOND EDITION TECHNISCHE
UNIVERSITAT DARMSTADT FACHBEREICH 10 * BIOLOGIE * BIBLIOTHEK *
SCHNITTSPAHNSTRAGE 10 0-64287 DARMSTADT FNV^NR. EDITED BY CARL W.
DIEFFENBACH NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
GABRIELA S. DVEKSLER UNIFORMED SERVICES UNIVERSITY OF THE HEALTH
SCIENCES COLD SPRING HARBOR LABORATORY PRESS COLD SPRING HARBOR, NEW
YORK CONTENTS PREFACE, XI 111 INTRODUCTION TO PCR, 1 1. SETTING UP A PCR
LABORATORY, 5 THEODORE E. MIFFLIN 2. DEALING WITH CARRYOVER
CONTAMINATION IN PCR: AN ENZYMATIC STRATEGY, 15 KYUSUNG PARK AND JUN LEE
3. HIGH-FIDELITY PCR ENZYMES, 21 HOLLY H. HOGREFE AND MICHAEL C. BOMS 4.
OPTIMIZATION AND TROUBLESHOOTING IN PCR, 35 KENNETH H. ROUX 5. PCR
AMPLIFICATION OF HIGHLY GC-RICH REGIONS, 43 LISE LOTTE HANSEN AND JUST
JUSTESEN 6. TIPS FOR LONG AND ACCURATE PCR, 53 WAYNE M. BARNES 7. PCR
PRIMER DESIGN, 61 ARUN APTE AND SAURABHA DANIEL 8. NONRADIOACTIVE CYCLE
SEQUENCING OF PCR-AMPLIFIED DNA, 75 SUSAN R. HAYNES IS SAMPLE
PREPARATION, 83 9. DNA PURIFICATION, 87 CRAIG SMITH, PAUL OTTO, REX
BITNER, AND GARY SHIELS 10. RNA PURIFICATION, 117 MICHAEL A. CONNOLLY,
PETER A. CLAUSEN, AND JAMES G. LAZAR VII VIII U CONTENTS 11. IN SITU
LOCALIZATION OF GENE EXPRESSION USING LASER CAPTURE MICRODISSECTION, 135
MOTOKO MORIMOTO, MASAHIRO MORIMOTO, JEANNETTE WHITMIRE, ROBERT A. STAR,
JOSEPH F. URBAN, JR., AND WILLIAM C. GAUSE 12. STRATEGIES FOR OVERCOMING
PCR INHIBITION, 149 PETER RADSTROM, CHARLOTTA LBFSTROM, MARIA LOVENKLEV,
RICKARD KNUTSSON, AND PETRA WOLFFS S RT-PCR METHODS, 163 13. THE USE OF
INTERNAL CONTROLS FOR RELATIVE AND COMPETITIVE QUANTITATIVE RT-PCR, 167
RENEE M. HOMER 14. COMPARISON OF FOUR REAL-TIME PCR DETECTION SYSTEMS:
BIO-RAD I-CYCLER, ABI 7700, ROCHE LIGHTCYCLER, AND THE CEPHEID
SMARTCYCLER, 187 KATHERINE E. TEMPLETON AND ERIC C.J. CLAAS 15. NEW
TECHNOLOGY FOR QUANTITATIVE PCR, 199 WOLFGANG KUSSER, SANDRINE
JAVORSCHI, AND MARTIN A. GLEESON 16. AMPLIFICATION OF RNA:
HIGH-TEMPERATURE REVERSE TRANSCRIPTION AND DNA AMPLIFICATION WITH A
MAGNESIUM-ACTIVATED THERMOSTABLE DNA POLYMERASE, 211 EDWARD S. SMITH,
ALVIN K. LI, ALICE M. WANG, DAVID H. GELFAND, AND THOMAS W. MYERS H
DETECTION OF PCR PRODUCTS: SPECIALIZED APPLICATIONS, 221 17. LOSS OF
HETEROZYGOSITY, A MULTIPLEX PCR METHOD TO DEFINE NARROW DELETED
CHROMOSOMAL REGIONS OF A TUMOR GENOME, 223 LISE LOTTE HANSEN AND JUST
JUSTESEN 18. SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS, 237 ANNE
E. JEDLICKA AND STEVEN R. KLEEBERGER 19. REVERSE TRANSCRIPTASE IN SITU
PCR, 251 GERARD J. NUOVO 20. SENSITIVE AND FAST MUTATION DETECTION BY
SOLID-PHASE CHEMICAL CLEAVAGE OF MISMATCHES, 265 LISE LOTTE HANSEN, JUST
JUSTESEN, ANDREANA LAMBRINAKOS, AND RICHARD G.H. COTTON EH ANALYSIS OF
DIFFERENTIAL GENE EXPRESSION BY PCR, 279 21. APPLYING PCR FOR
MICROARRAY-BASED GENE EXPRESSION ANALYSIS, 281 JOAN M. ZAKEL 22.
IDENTIFICATION OF DIFFERENTIAL GENES BY SUPPRESSION SUBTRACTIVE
HYBRIDIZATION, 297 DENIS V. REBRIKOV CONTENTS * IX 23. FLUORESCENT MRNA
DIFFERENTIAL DISPLAY IN GENE EXPRESSION ANALYSIS, 327 JEFFREY S. FISHER,
JONATHAN MEADE, JAMIE WALDEN, ZHEN GUO, AND PENG LIANG B CLONING BY PCR,
349 24. PCR-BASED METHOD FOR SCREENING LIBRARIES, 351 DAVID I. ISRAEL
25. BEYOND CLASSIC RACE (RAPID AMPLIFICATION OF CDNA ENDS), 359 MICHAEL
A. FROHMAN 26. SYNTHESIS AND ANALYSIS OF DNA LIBRARIES USING PCR, 391
JOHN L. JOYAL, JINWEI JIANG, AND DAVID I. ISRAEL I * CLONING OF PCR
PRODUCTS, 403 27. STRATEGIES FOR CLONING PCR PRODUCTS, 405 PETER D ARPA
28. BIDIRECTIONAL AND DIRECTIONAL CLONING OF PCR PRODUCTS, 421 GINA L.
COSTA AND MICHAEL P. WEINER 29. RIBOCLONING: DNA CLONING AND GENE
CONSTRUCTION USING PCR PRIMERS TERMINATED WITH A RIBONUCLEOTIDE, 441
WAYNE M. BARNES ._B_ MUTAGENESIS BY PCR, 451 30. MUTAGENIC PCR, 453 R.
CRAIG CADWELL AND GERALD F. JOYCE 31. RAPID PCR SITE-DIRECTED
MUTAGENESIS, 459 MICHAEL P. WEINER AND GINA L. COSTA 32. MUTAGENESIS AND
SYNTHESIS OF NOVEL RECOMBINANT GENES USING PCR, 467 ABBE N. VALLEJO,
ROBERT J. POGULIS, AND LARRY R. PEASE 33. STREAMLINED GENE ASSEMBLY PCR,
475 WAYNE M. BARNES AND ELAINE R. FRAWLEY APPENDICES 1. LICENSING, 483
2. MODIFICATION OF OLIGONUCLEOTIDES FOR USE IN PCR, 485 3. CAUTIONS, 491
4. SUPPLIERS, 503 INDEX, 505
|
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| indexdate | 2024-07-09T19:14:36Z |
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| isbn | 0879696532 0879696540 |
| language | English |
| lccn | 2003053085 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010352011 |
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| spelling | PCR primer a laboratory manual ed. by Carl W. Dieffenbach ... 2. ed. Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press 2003 XI, 520 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s Primer Molekulargenetik (DE-588)4318719-5 s Methode (DE-588)4038971-6 s DE-604 Dieffenbach, Carl W. Sonstige oth HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010352011&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | PCR primer a laboratory manual Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4318719-5 (DE-588)4256726-9 (DE-588)4038971-6 |
| title | PCR primer a laboratory manual |
| title_auth | PCR primer a laboratory manual |
| title_exact_search | PCR primer a laboratory manual |
| title_full | PCR primer a laboratory manual ed. by Carl W. Dieffenbach ... |
| title_fullStr | PCR primer a laboratory manual ed. by Carl W. Dieffenbach ... |
| title_full_unstemmed | PCR primer a laboratory manual ed. by Carl W. Dieffenbach ... |
| title_short | PCR primer |
| title_sort | pcr primer a laboratory manual |
| title_sub | a laboratory manual |
| topic | Biologia molecular larpcal Expressão gênica larpcal Genética molecular larpcal Genética larpcal Mutagênese larpcal Reação em cadeia por polimerase larpcal Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik (DE-588)4318719-5 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biologia molecular Expressão gênica Genética molecular Genética Mutagênese Reação em cadeia por polimerase Réaction en chaîne de la polymérase - Manuels de laboratoire Polymerase Chain Reaction Laboratory Manuals Polymerase chain reaction Laboratory manuals Primer Molekulargenetik Polymerase-Kettenreaktion Methode |
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