Membrane protein protocols: expression, purification, and characterization
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2003
|
| Schriftenreihe: | Methods in molecular biology
228 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 334 S. Ill., graph. Darst. |
| ISBN: | 1588291243 |
Internformat
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| 245 | 1 | 0 | |a Membrane protein protocols |b expression, purification, and characterization |c edited by Barry S. Selinsky |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2003 | |
| 300 | |a XIV, 334 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 228 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Eiwitten |2 gtt | |
| 650 | 7 | |a Membranen |2 gtt | |
| 650 | 7 | |a Proteínas da membrana |2 larpcal | |
| 650 | 4 | |a Protéines membranaires - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Membrane Proteins |v Laboratory Manuals | |
| 650 | 4 | |a Membrane proteins |v Laboratory manuals | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-010257931 | ||
Datensatz im Suchindex
| _version_ | 1804129902605107200 |
|---|---|
| adam_text | Contents
Preface
.............................................................................................................
v
Contributors
.....................................................................................................
¡χ
Part I. Expression of Membrane Proteins
1
Expression and Purification of the Amphipathic Form
of Rabbit Cytochrome b5 in Escherichia
coli
LucyWaskell
..........................................................................................3
2
Dihydroorotate Dehydrogenase of Escherichia
coli
Kaj
Frank Jensen and Sine Larsen
................................................... 11
3
A General Approach for Heterologous Membrane Protein
Expression in Escherichia
coli:
The Uncoupling Protein,
UCPI,
as an Example
Alison Z. Shaw and Bruno Miroux
.....................................................23
4
Expression of Membrane-Bound Iron-Sulfur Proteins
Dirk Schneider, Christian
L
Schmidt,
and Andreas
Seidler........................................................................37
5
Heterologous Expression of Human Scavenger Receptor
Class
В
Type I (SR-BI) in Pichia
pastoris
Chang-Hoon Han,
Moritz Werder,
Wilk
von Gustedt,
and Helmut Hauser..........................................................................49
6 Expression
of Oligomeric
Amiloride-Sensitive
Epithelial
Sodium
Channel in Sf9
Insect Cells
U.
Subrahmanyeswara
Rao
................................................................65
7
Functional Expression of His-Tagged Rhodopsin Sf9 Insect Cells
G
iel
J.C.
G.M. Bosman, Jenny van Oostrum,
Githa Breikers, Petra H.M. Bovee-Geurts,
Corne
H.W.
Klaassen, and
Willem J.
DeGrip
...............................73
Part
II. Detergent Selection in Membrane Protein Purification
8
Preparation of Glycerol Facilitator for Protein Structure
and Folding Studies in Solution
Darren Manley and Joe D. O Neil
.......................................................89
9
Solubilization of Chemokine Receptors from Cell Membranes
Robert Staudinger and Juan
С
Bandrés........................................
103
VII
viii Contents
1
0
A Systematic Approach for the Solubilization of the Integral
Membrane Protein Lysophospholipid: Acyl-CoA
Acyltransferase
(LAT)
Claus Kerkhoff
and
Volkhard
Kaever
.............................................. 111
Part III. Membrane Protein Purification and
Reconstitution
11
Purification of Omp50, a Minor Porin of Campylobacter jejuni
Jean Michel
Bolla
............................................................................... 131
12
Purification of Porins from Mycobacterium smegmatis
Christian Heinz, Eva Roth, and Michael
Niederweis..................... 139
13
Isolation of the Melanocortin
5
Receptor: From cDNA Sequence
to Isolating an Integral Membrane Protein
Benjamin L. Clarke
............................................................................ 151
14
Purification of Mammalian
Serine
Palmitoyltransferase, a Hetero-
Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide
Chromatography
Kentaro Hanada and Masahiro Nishijima
.......................................163
15
Purification of Phosphatidylglycerophosphate Synthase
from Cultured Mammalian Cells
Kiyoshi Kawasaki and Masahiro Nishijima
....................................175
16
Purification of Pancreastatin Receptor from Rat Liver Membranes
Víctor Sánchez-Margalet, José
Santos-Alvarez,
and Sandra
Díaz-Troya
.................................................................187
17
Purification,
Reconstitution,
and Functional Characterization
of Zinc Transporter from Rat Renal Brush Border Membranes
Rajendra
Prasad
................................................................................195
18
Isolation of
Lipid
Raft-Associated Proteolipids
Jaime
Millán, María
С.
de Marco, Mohammed Qaidi,
Alicia Batista,
Fernando
Martín-Belmonte,
and Miguel A.
Alonso
....................................................................223
19
Purification
of Membrane-Bound Catechol-O-Methyltransferase
Maria
João Bonifácio
and
Patrício Soares-da-Silva
.....................231
20
Purification and Characterization of Transporter Proteins
from Human Erythrocyte Membrane
Da-Neng Wang, M. Joanne Lemieux,
and Jonathan M. Boulter
..............................................................239
21
Purification of the Human Erythrocyte PS-Stimulated
Mg2+-ATPase: A Putative PS Flippase
Jill V. Lyles, Kathleen Comely-Moss, Christine M. Smith,
and David J.
Daleke
.......................................................................257
Contents ix
22
Reconstitution
and Assay of Biogenic Membrane-Derived
Phospholipid Flippase Activity in Proteoliposomes
Sathyanarayana
N.
Gummadi,
Sigrún Hrafnsdóttir,
Jane Walent, William
E. Watkins,
and Anant K. Menon
......................................................................271
23
Expression, Purification, and
Reconstitution
of Rat Liver
Carnitine Palmitoyltransferase I
Nicholas F. Brown
.............................................................................281
Part IV. Structural Analysis of Membrane Proteins
24
Crystallization in
Lipidie
Cubic Phases: A Case Study with
Bacteriorhodopsin
Valentin I. Gordeliy,
Ramona
Schlesinger, Rouslan Efremov,
Georg Büldt,
and Joachim Heberle
.............................................305
25
Optical Biosensor Assay Using Retroviral Receptor
Pseudotypes
Joseph Rucker
...................................................................................317
Index
............................................................................................................329
|
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| indexdate | 2024-07-09T19:12:31Z |
| institution | BVB |
| isbn | 1588291243 |
| language | English |
| lccn | 2003041743 |
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| physical | XIV, 334 S. Ill., graph. Darst. |
| publishDate | 2003 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Membrane protein protocols expression, purification, and characterization edited by Barry S. Selinsky Totowa, N.J. Humana Press 2003 XIV, 334 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 228 Includes bibliographical references and index Eiwitten gtt Membranen gtt Proteínas da membrana larpcal Protéines membranaires - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Membrane Proteins Laboratory Manuals Membrane proteins Laboratory manuals Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Membranproteine (DE-588)4130026-9 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Selinsky, Barry S. Sonstige oth Methods in molecular biology 228 (DE-604)BV035362695 228 Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010257931&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Membrane protein protocols expression, purification, and characterization Methods in molecular biology Eiwitten gtt Membranen gtt Proteínas da membrana larpcal Protéines membranaires - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Membrane Proteins Laboratory Manuals Membrane proteins Laboratory manuals Untersuchungsmethode (DE-588)4324907-3 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4324907-3 (DE-588)4130026-9 (DE-588)4143413-4 |
| title | Membrane protein protocols expression, purification, and characterization |
| title_auth | Membrane protein protocols expression, purification, and characterization |
| title_exact_search | Membrane protein protocols expression, purification, and characterization |
| title_full | Membrane protein protocols expression, purification, and characterization edited by Barry S. Selinsky |
| title_fullStr | Membrane protein protocols expression, purification, and characterization edited by Barry S. Selinsky |
| title_full_unstemmed | Membrane protein protocols expression, purification, and characterization edited by Barry S. Selinsky |
| title_short | Membrane protein protocols |
| title_sort | membrane protein protocols expression purification and characterization |
| title_sub | expression, purification, and characterization |
| topic | Eiwitten gtt Membranen gtt Proteínas da membrana larpcal Protéines membranaires - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Membrane Proteins Laboratory Manuals Membrane proteins Laboratory manuals Untersuchungsmethode (DE-588)4324907-3 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Eiwitten Membranen Proteínas da membrana Protéines membranaires - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Membrane Proteins Laboratory Manuals Membrane proteins Laboratory manuals Untersuchungsmethode Membranproteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010257931&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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