Bone research protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2003
|
| Schriftenreihe: | Methods in molecular medicine
80 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 448 S. Ill., graph. Darst. |
| ISBN: | 1588290441 |
Internformat
MARC
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| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2003 | |
| 300 | |a XIV, 448 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular medicine |v 80 | |
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| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Bone and Bones |x cytology | |
| 650 | 4 | |a Bone cells |v Laboratory manuals | |
| 650 | 4 | |a Bones |x Cultures and culture media |v Laboratory manuals | |
| 650 | 4 | |a Cell Culture Techniques | |
| 650 | 4 | |a Histological Techniques | |
| 650 | 4 | |a Osteoblasts | |
| 650 | 4 | |a Osteoclasts | |
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Datensatz im Suchindex
| _version_ | 1804129888952647680 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I Culture of Osteoblasts and Osteocytes
1 Human Osteoblast Culture
James A. Gallagher 3
2 Osteoblast Isolation from Murine Calvariae and Long Bones
Astrid Bakker and Jenneke Klein Nulend 19
3 Mineralizing Fibroblast Colony Forming Assays
Andrew Scutt, Lynsey Reading, Nanette Scutt,
and Karen Still 29
4 Osteocyte Isolation and Culture
Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas,
and Jenneke Klein Nulend 41
Part II Culture of Osteoclasts
5 Isolated Osteoclast Cultures
Astrid Hoebertz and Timothy R. Arnett 53
6 Primary Isolation and Culture of Chicken Osteoclasts
Patricia Collin Osdoby, Fred Anderson,
and Philip Osdoby 65
7 Isolation and Purification of Rabbit Osteoclasts
Fraser P. Coxon, Julie C. Frith, Helena L. Benford,
and Michael J. Rogers 89
8 Generating Human Osteoclasts from Peripheral Blood
Afsie Sabokbarand Nicholas A. Athanasou 707
9 Generating Human Osteoclasts from Bone Marrow and
Peripheral Blood
Adrienne M. Flanagan and Helen M. Massey 773
10 Generating Murine Osteoclasts from Bone Marrow
Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka,
and Tatsuo Suda 129
vii
viii Contents
11 Osteoclast Formation in the Mouse Coculture Assay
Robert J. van t Hof. 145
12 RANKL Mediated Osteoclast Formation
from Murine RAW 264.7 Cells
Patricia Collin Osdoby, Xuefeng Yu, Hong Zheng,
and Philip Osdoby 153
Part III Bone Organ Culture
13 Analysis of Osteoclast Function in Mouse Calvarial Cultures
Michael J. Marshall and Marit N. Rowlands 169
14 Assessing Bone Formation
Using Mouse Calvarial Organ Culture
/. Ross Garrett 183
Part IV Histological and Ultrastructural Techniques
15 In Situ Hybridization and In Situ Reverse Transcription
Polymerase Chain Reaction in Human Bone Sections
Andrew P. Mee and Judith A. Hoyland 201
16 Techniques for the Study of Apoptosis in Bone
Brendon S. Noble and Hazel Y. Stevens 225
17 Protein Localization in Wax Embedded and Frozen Sections
of Bone Using Immunohistochemistry
Sharyn Bord 237
18 Detection of Noncollagenous Bone Proteins
in Methylmethacrylate Embedded Human Bone Sections
Johannes P. T. M. van Leeuwen and Pieter Derkx 249
19 Fluorescence Imaging of Bone Resorbing Osteoclasts
by Confocal Microscopy
Stephen A. Nesbitt and Michael A. Horton 259
20 Bone Histomorphometry
Shobna Vedi and Juliet Compston 283
21 Transmission Electron Microscopy of Bone
Vincent Everts, Anneke Niehof, and Wouter Beertsen 299
22 Scanning Electron Microscopy of Bone
Deborah Marshall, Miep H. Helfrich,
and Richard M. Aspden 311
Contents ix
Part V Imaging Techniques
23 Bone Measurements by Peripheral Quantitative
Computed Tomography in Rodents
Jiirg A. Gasser 323
Part VI In Vivo Techniques
24 Studies of Local Bone Remodeling:
The Calvarial Injection Assay
Robert J. van t Hof, Claire E. Clarkin,
and Kenneth J. Armour 345
25 Inflammation Induced Osteoporosis: The IMO Model
Kenneth J. Armour and Katharine E. Armour 353
26 Ovariectomy and Estrogen Replacement in Rodents
Jade W. M. Chow 361
Part VII Mechanical Loading Techniques
27 Mechanical Testing of Bone Ex Vivo
Richard M. Aspden 369
28 Bone Cell Responses to Fluid Flow
Hazel Y. Stevens and John A. Frangos 381
29 Methods for Analyzing Bone Cell Responses to Mechanical
Loading Using In Vitro Monolayer and Organ Culture Models
Andrew A. Pitsillides, Victoria Das Gupta,
Dominic Simon, and Simon C. F. Rawlinson 399
Part VIII Molecular Analysis
30 Extraction of Nucleic Acids from Bone
Tracy L. Stewart and Val Mann 425
31 Analysis of Gene Expression in Bone by Quantitative RT PCR
Deborah Ireland 433
Index 441
|
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| id | DE-604.BV016969297 |
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| indexdate | 2024-07-09T19:12:18Z |
| institution | BVB |
| isbn | 1588290441 |
| language | English |
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| physical | XIV, 448 S. Ill., graph. Darst. |
| publishDate | 2003 |
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| publishDateSort | 2003 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Bone research protocols ed. by Miep H. Helfrich ... Totowa, N.J. Humana Press 2003 XIV, 448 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 80 Includes bibliographical references and index Bot (anatomie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Bone and Bones cytology Bone cells Laboratory manuals Bones Cultures and culture media Laboratory manuals Cell Culture Techniques Histological Techniques Osteoblasts Osteoclasts Knochen (DE-588)4128031-3 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Gidsen (vorm) gtt Knochen (DE-588)4128031-3 s Molekulare Medizin (DE-588)4543844-4 s DE-604 Helfrich, Miep H. Sonstige oth Methods in molecular medicine 80 (DE-604)BV011334960 80 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010248123&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bone research protocols Methods in molecular medicine Bot (anatomie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Bone and Bones cytology Bone cells Laboratory manuals Bones Cultures and culture media Laboratory manuals Cell Culture Techniques Histological Techniques Osteoblasts Osteoclasts Knochen (DE-588)4128031-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| subject_GND | (DE-588)4128031-3 (DE-588)4543844-4 |
| title | Bone research protocols |
| title_auth | Bone research protocols |
| title_exact_search | Bone research protocols |
| title_full | Bone research protocols ed. by Miep H. Helfrich ... |
| title_fullStr | Bone research protocols ed. by Miep H. Helfrich ... |
| title_full_unstemmed | Bone research protocols ed. by Miep H. Helfrich ... |
| title_short | Bone research protocols |
| title_sort | bone research protocols |
| topic | Bot (anatomie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Bone and Bones cytology Bone cells Laboratory manuals Bones Cultures and culture media Laboratory manuals Cell Culture Techniques Histological Techniques Osteoblasts Osteoclasts Knochen (DE-588)4128031-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| topic_facet | Bot (anatomie) Wetenschappelijke technieken Wissenschaftliches Arbeiten Bone and Bones cytology Bone cells Laboratory manuals Bones Cultures and culture media Laboratory manuals Cell Culture Techniques Histological Techniques Osteoblasts Osteoclasts Knochen Molekulare Medizin Gidsen (vorm) |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010248123&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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