Physiological response of Lactococcus lactis to high pressure:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2002 |
| Beschreibung: | 104 Bl. Ill., graph. Darst. |
Internformat
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| 300 | |a 104 Bl. |b Ill., graph. Darst. | ||
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| 650 | 0 | 7 | |a Konservierung |0 (DE-588)4114279-2 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1816445038289223680 |
|---|---|
| adam_text |
CONTENTS
1. INTRODUCTION.1
1.1 GENERAL PRINCIPLE OF HIGH-PRESSURE.1
1.2 EFFECTS OF HIGH-PRESSURE ON MICROORGANISMS.4
1.2.1 MECHANISMS AND LIMITS OF ADAPTATION.4
1.2.2 MECHANISMS OF INACTIVATION.5
1.3 EFFECTS AND INTERACTIONS OF TREATMENT VARIABLES.7
1.3.1 MICROORGANISMS.7
1.3.2 PRESSURE LEVEL AND TIME.8
1.3.3 TEMPERATURE. 9
1.3.4 PH.9
1.3.5 COMPOSITION OF MEDIUM. 10
1.3.6 ANTIMICROBIAL COMPOUNDS.10
1.4 KINETICS OF MICROBIAL INACTIVATION.11
1.5 AIM OF THIS THESIS.13
2. MATERIAL AND METHODS.14
2.1.1 ABREVIATIONS AND SOLUTIONS.14
2.1.2 CHEMICALS. 14
2.2 BACTERIAL STRAINS AND MEDIA.14
2.3.1 MILK BUFFER.15
2.3.2 MILK FILTRATE.15
2.4 DETERMINATION OF WATER ACTIVITY (A
W) OF MILK BUFFERS.15
2.5 PRESSURIZATION OF CELL SUSPENSIONS.15
2.6 METHODS FOR CHARACTERIZATION OF PRESSURE-INDUCED INACTIVATION ON
CELLS.15
2.6.1 ENUMERATION OF VIABLE CELLS.16
2.6.2 MEMBRANE INTEGRITY ASSAY. 16
2.6.3 METABOLIC ACTIVITY ASSAY.17
2.6.4 LMRP ACTIVITY ASSAY.17
2.6.5 MEASUREMENT OF DETECTION TIME OF GROWTH.17
2.7 METHODS FOR CHARACTERIZATION OF GROWTH UNDER HIGH-PRESSURE
CONDITIONS.18
2.7.1 DETERMINATION OF MAXIMAL PRESSURE OF GROWTH.18
2.7.2 DETERMINATION OF GROWTH RATD UNDER PRESSURE.18
2.7.3 MEASUREMENTS OF OPTICAL DENSITY.18
2.7.4 MICROSCOPIC COUNTS OF CELLS.19
2.7.5 DETERMINATION OF MAXIMAL AND OPTIMAL TEMPERATURE OF GROWTH.19
2.7.6 INDIRECT IMMUNOFLUORESCENCE MICROSCOPY.19
2.8 METHOD FOR MEASUREMENT OF INTERNAL PH.,.19
2.8.1 LABELING OF CELLS WITH CFSE FOR PHJ
N DETERMINATION.19
2.8.2 OFFLINE MEASUREMENT OF PHJN AT AMBIENT PRESSURE.20
2.8.3 IN-SITU MEASUREMENT OF PH;YY.20
2.8.4 REVERSIBILITY TEST.21
2.8.5 COMPUTATION OF THE PH-VALUES DURING PRESSURE TREATMENT.21
2.9 METHODS FOR MEASUREMENT OF MEMBRANE PHASE STATE.22
2.9.1 DETERMINATION OF TEMPERATURE-DEPENDENT PHASE STATE OF THE MEMBRANE
BY FT-IR
SPECTROSCOPY.22
2.9.2 DETERMINATION OF THE TEMPERATURE AND PRESSURE-DEPENDENT PHASE
STATE OF THE
MEMBRANE BY FLUORESCENCE SPECTROSCOPY.23
2.10 DETERMINATION OF ACCUMULATED OSMOLYTES AFTER HYPER-OSMOTIC SHOCK.23
2.10.1 CELL EXTRACT.23
2.10.2 DETERMINATION OF ACCUMULATED OSMOLYTES BY HPLC ANALYSIS OF CELL
EXTRACTS.24
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/966630408
3. RESULTS.25
3.1 GROWTH UNDER HIGH-PRESSURE CONDITIONS.25
3.1.1 EFFECT OF TEMPERATURE AND PRESSURE ON GROWTH RATE.26
3.1.2 MORPHOLOGIC CHANGES AND IMMUNOFLUORESCENCE MICROSCOPY.29
3.2 PRESSURE INACTIVATION. DEVELOPMENT OF MODEL SYSTEM AND STRAIN
SELECTION.37
3.2.1 VALIDATION OF MILK BUFFER AS FOOD SYSTEM.37
3.2.2 SELECTION OF STRAIN.39
3.3 DETECTION TIME.41
3.4 EFFECT OF SUCROSE AND SODIUM CHLORIDE ON THE SURVIVAL AND METABOLIC
ACTIVITY OF
LACTOCOCCUS LACTIS UNDER HIGH-PRESSURE CONDITIONS.,.43
3.4.1 INACTIVATION OF LACTOCOCCUS LACTIS CELLS IN MILK BUFFER AT
DIFFERENT PRESSURE LEVELS .43
3.4.2 METABOLIC ACTIVITY AND MEMBRANE INTEGRITY AFTER HIGH-PRESSURE
TREATMENTS.44
3.4.3 INACTIVATION KINETICS OF LACTOCOCCUS LACTIS IN MILK BUFFER AND
MILK BUFFER WITH
ADDITIVES.46
3.4.4 EFFECT OF LOW MOLECULAR SOLUTES ON METABOLIC ACTIVITY AND MEMBRANE
INTEGRITY.49
3.5 IN-SITU DETERMINATION OF INTRACELLULAR PH OF LACTOCOCCUS LACTIS AND
LACTOBACILLUS
PLANTARUM DURING PRESSURE TREATMENT.51
3.5.1 INACTIVATION OF LACTOCOCCUS LACTIS IN MILK BUFFER WITH DIFFERENT
PH.51
3.5.2 INACTIVATION OF
LACTOBACILLUS PLANTARUM IN MILK BUFFER WITH DIFFERENT PH.53
3.5.3 EFFECT OF THE BUFFER PH (PHEX) ON THE INTERNAL PH (PHJYY).54
3.5.4 CALIBRATION CURVE FOR MEASURING PHJN UNDER HIGH-PRESSURE.55
3.5.5 EFFECT OF HIGH-PRESSURE ON THE INTERNAL PH OF LACTOCOCCUS
LACTIS.56
3.5.6 EFFECT OF HIGH-PRESSURE ON THE INTERNAL PH OF LACTOBACILLUS
PLANTARUM.60
3.5.7 EFFECT OF HIGH-PRESSURE ON THE INTRACELLULAR PH OF LACTOCOCCUS
LACTIS IN PRESENCE OF
OSMOLYTES.63
3.6 INVESTIGATION OF MECHANISMS OF BAROPROTECTION OF SODIUM CHLORIDE AND
SUCROSE.64
3.6.1 COMPARISON OF INACTIVATION KINETIKS OF LACTOCOCCUS LACTIS IN
PRESENCE OF DIFFERENT
SOLUTES.64
3.6.2 LMRP ACTIVITY OF PRESSURIZED CELLS IN MILK BUFFER AND MILK BUFFER
WITH ADDITIVES.67
3.6.3 INTRACELLULAR ACCUMULATION OF OSMOLYTES BY OSMOTIC UPSHOCK.68
3.6.4 EFFECT OF OSMOLYTES ON THE MEMBRANE PHASE STATE AT DIFFERENT
TEMPERATURES MEASURED
BY FT-IR SPECTROSCOPY.69
3.6.5 EFFECT OF OSMOLYTES ON THE MEMBRANE PHASE STATE AT DIFFERENT
TEMPERATURES AND
PRESSURES AS MEASURED BY LAURDAN FLUORESCENCE SPECTROSCOPY.70
4. DISCUSSION.73
4.1 EFFECT OF HIGH-PRESSURE DURING GROWTH.74
4.2 EFFECTS OF HIGH-PRESSURE DURING INACTIVATION. 76
4.3 GENERAL REMARKS.84
5. SUMMARY.86
6. ZUSAMMENFASSUNG.89
7. REFERENCES
92 |
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| author | Molina-Höppner, Adriana 1972- |
| author_GND | (DE-588)124301967 |
| author_facet | Molina-Höppner, Adriana 1972- |
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| author_sort | Molina-Höppner, Adriana 1972- |
| author_variant | a m h amh |
| building | Verbundindex |
| bvnumber | BV016961710 |
| classification_tum | LEB 123d |
| ctrlnum | (OCoLC)642934959 (DE-599)BVBBV016961710 |
| discipline | Lebensmitteltechnologie |
| format | Book |
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| indexdate | 2024-11-22T17:36:19Z |
| institution | BVB |
| language | English |
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| physical | 104 Bl. Ill., graph. Darst. |
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| spelling | Molina-Höppner, Adriana 1972- Verfasser (DE-588)124301967 aut Physiological response of Lactococcus lactis to high pressure Adriana Molina-Höppner 2002 104 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2002 Hochdruck (DE-588)4160091-5 gnd rswk-swf Lebensmittel (DE-588)4034870-2 gnd rswk-swf Zelltod (DE-588)4207415-0 gnd rswk-swf Konservierung (DE-588)4114279-2 gnd rswk-swf Lactococcus lactis (DE-588)4183661-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Lactococcus lactis (DE-588)4183661-3 s Hochdruck (DE-588)4160091-5 s Zelltod (DE-588)4207415-0 s DE-604 Lebensmittel (DE-588)4034870-2 s Konservierung (DE-588)4114279-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010245051&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molina-Höppner, Adriana 1972- Physiological response of Lactococcus lactis to high pressure Hochdruck (DE-588)4160091-5 gnd Lebensmittel (DE-588)4034870-2 gnd Zelltod (DE-588)4207415-0 gnd Konservierung (DE-588)4114279-2 gnd Lactococcus lactis (DE-588)4183661-3 gnd |
| subject_GND | (DE-588)4160091-5 (DE-588)4034870-2 (DE-588)4207415-0 (DE-588)4114279-2 (DE-588)4183661-3 (DE-588)4113937-9 |
| title | Physiological response of Lactococcus lactis to high pressure |
| title_auth | Physiological response of Lactococcus lactis to high pressure |
| title_exact_search | Physiological response of Lactococcus lactis to high pressure |
| title_full | Physiological response of Lactococcus lactis to high pressure Adriana Molina-Höppner |
| title_fullStr | Physiological response of Lactococcus lactis to high pressure Adriana Molina-Höppner |
| title_full_unstemmed | Physiological response of Lactococcus lactis to high pressure Adriana Molina-Höppner |
| title_short | Physiological response of Lactococcus lactis to high pressure |
| title_sort | physiological response of lactococcus lactis to high pressure |
| topic | Hochdruck (DE-588)4160091-5 gnd Lebensmittel (DE-588)4034870-2 gnd Zelltod (DE-588)4207415-0 gnd Konservierung (DE-588)4114279-2 gnd Lactococcus lactis (DE-588)4183661-3 gnd |
| topic_facet | Hochdruck Lebensmittel Zelltod Konservierung Lactococcus lactis Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010245051&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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