Nitrogen partitioning in plants: the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Heringen, Am guten Born 2
<<D.>> Dietrich
2002
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Tübingen, Univ., Diss., 2002 |
| Physical Description: | IV, 85 S. Ill., graph. Darst. : 21 cm |
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MARC
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| 100 | 1 | |a Dietrich, Daniela |d 1973- |e Verfasser |0 (DE-588)124371043 |4 aut | |
| 245 | 1 | 0 | |a Nitrogen partitioning in plants |b the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana |c vorgelegt von Daniela Dietrich |
| 264 | 1 | |a Heringen, Am guten Born 2 |b <<D.>> Dietrich |c 2002 | |
| 300 | |a IV, 85 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Tübingen, Univ., Diss., 2002 | ||
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Record in the Search Index
| _version_ | 1808226294024896512 |
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| adam_text |
TABLE
OF
CONTENTS
ABSTRACT
.
V
ZUSAMMENFASSUNG
.
VI
1
INTRODUCTION
.
1
1.1
NITROGEN
NUTRITION
OF
PLANTS
.
1
1.2
NITROGEN
ASSIMILATION
AND
TRANSPORT
.
1
1.3
PLANT
PEPTIDES
.
2
1.3.1
PEPTIDES
WITH
A
SPECIFIC
STRUCTURE
.
3
1.3.2
PEPTIDES
AS
METABOLIC
INTERMEDIATES
.
4
1.4
PHYSIOLOGICAL
RELEVANCE
OF
PEPTIDES
PRODUCED
BY
PROTEIN
DEGRADATION
.
4
1.5
PHYSIOLOGICAL
CHARACTERISATION
OF
PLANT
PEPTIDE
TRANSPORT
.
6
1.6
PEPTIDE
TRANSPORTERS
.
7
1.6.1
THE
ABC
FAMILY
.
7
1.6.2
THE
OPT
FAMILY
.
8
1.6.3
THE POT
FAMILY
.
9
1.7
RESEARCH
OBJECTIVES
.
12
2
MATERIALS
AND
METHODS
.
13
2.1
ABBREVIATIONS
.
13
2.2
CHEMICALS
AND
ENZYMES
.
13
2.3
OLIGONUCLEOTIDES
.
14
2.4
BACTERIAL
STRAINS,
YEAST
STRAINS,
PLANT
MATERIAL
AND
VECTORS
.
17
2.4.1
BACTERIAL
AND
YEAST
STRAINS
.
17
2.4.2
PLANT
MATERIAL
.
17
2.4.3
VECTORS
.
17
2.5
MEDIA
.
17
2.5.1
MEDIA
FOR
E.
COLI AND
A.
TUMEFACIENS
.
17
2.5.2
MEDIA
FOR
SACCHAROMYCES
CEREVISIAE
.
18
2.5.3
MEDIA
FOR
ARABIDOPSIS
THALIANA
.
18
2.6 PLANT
GROWTH
CONDITIONS
.
19
2.7
CROSSING
OF
PLANTS
.
20
TABLE
OF
CONTENTS
2.8
STANDARD
MOLECULAR
BIOLOGY
METHODS
.
20
2.9
REVERSE
TRANSCRIPTASE
REACTIONS
.
20
2.10
CONSTRUCTS
FOR
YEAST
COMPLEMENTATION
.
20
2.11
TRANSFORMATION
OF
SACCHAROMYCES
CEREVISIAE
.
21
2.12
UPTAKE
EXPERIMENTS
.
21
2.13
PHASEOLOTOXIN
BIOASSAY
.
21
2.14
PROMOTER-P-GLUCURONIDASE
FUSION
CONSTRUCTS
FOR
PLANT
TRANSFORMATION
.
22
2.15
TRANSFORMATION
OF
ARABIDOPSIS
THALIANA
.
22
2.16
ANALYSIS
OF
0-GLUCURONIDASE
ACTIVITY
.
23
2.16.1
STAINING
OF
PLANT
MATERIAL
.
23
2.16.2
EMBEDDING
AND
SECTIONING
OF
PLANT
MATERIAL
.
23
2.17
RNA
ISOLATION
.
24
2.17.1
RNA
ISOLATION
.
24
2.17.2
RNA
ISOLATION
FROM
SENESCENT
PLANT
MATERIAL
.
24
2.18
RNA
GEL
BLOT
ANALYSIS
.
25
2.19
MACRO-ARRAY
HYBRIDISATION
.
25
2.19.1
PREPARATION
OF
THE
MACRO-ARRAY
.
25
2.19.2
RADIOACTIVE
FIRST
STRAND
SYNTHESIS
.
25
2.19.3
HYBRIDISATION
.
26
2.20
IDENTIFICATION
OF
T-DNA
INSERTION
LINES
.
26
2.21
ANALYSIS
OF
T-DNA
INSERTION
LINES
.
27
2.21.1
GROWTH
ON
PEPTIDES
AS
SOLE
SOURCE
OF
NITROGEN
.
27
2.21.2
HYDROPONIC
CULTURE
OF
ARABIDOPSIS
THALIANA
.
27
3
RESULTS
.
28
3.1
ISOLATION
AND
CHARACTERISATION
OF
PEPTIDE
TRANSPORTERS
FROM
ARABIDOPSIS
THALIANA
.
28
3.1.1
ISOLATION
OF
ATNTR2,
A
NEW
PEPTIDE
TRANSPORTER
FROM
ARABIDOPSIS
THALIANA,
BY
YEAST
COMPLEMENTATION
.
28
3.1.2
ISOLATION
OF
PUTATIVE
PEPTIDE
TRANSPORTERS
FROM
ARABIDOPSIS
THALIANA
BY
RT-PCR.
30
3.1.3
PHASEOLOTOXIN,
A
TOXIC
PEPTIDE
ANALOGUE,
IS
TRANSPORTED
BY
ATNTRL
AND
ATNTR2.
34
3.2
CHARACTERISATION
OF
VFPTRL,
A
PEPTIDE
TRANSPORTER
FROM
VICIAFABA
.
37
3.3
ANALYSIS
OF
ATNTR2
EXPRESSION
BY
RNA
GEL
BLOT
ANALYSIS
.
39
3.4
EXPRESSION
ANALYSIS
OF
ATNTRL,
ATNTR2
AND
OTHER
PUTATIVE
PEPTIDE
TRANSPORTERS
BY
N
PROMOTER-REPORTERGENE
FUSION
.
40
3.4.1
ISOLATION
AND
CLONING
OF
PROMOTERS
.
40
3.4.2
ANALYSIS
OF
PROMOTER
SEQUENCES
FOR
CIS-ACTING
ELEMENTS
REGULATING
DEVELOPMENTAL
AND
TISSUE-SPECIFIC
EXPRESSION
.
40
3.4.3
P-GLUCURONIDASE
ACTIVITY
UNDER
THE
CONTROL
OF
THE
ATNTRL
PROMOTER
.
42
3.4.4
P-GLUCURONIDASE
ACTIVITY
UNDER
THE
CONTROL
OF
THE
ATNTR2
PROMOTER
.
43
3.4.5
P-GLUCURONIDASE
ACTIVITY
UNDER
THE
CONTROL
OF
THE
AT2G02020
(FI4)
PROMOTER
.
44
3.4.6
P-GLUCURONIDASE
ACTIVITY
UNDER
THE
CONTROL
OF
THE
ATLG62200
(F19)
PROMOTER
.
45
3.5
EXPRESSION
OF
PEPTIDE
TRANSPORTER
GENES
DURING
SENESCENCE
.
45
3.6
ISOLATION
OF
T-DNA
INSERTION
LINES
FOR
MEMBERS
OF
THE
POT
FAMILY
.
47
3.6.1
CHARACTERIZATION
OF
ATNTRL
MUTANTS
.
48
3.6.1.1
INTEGRATION
OF
THE
T-DNAS
IN
ATNTRL
.
48
3.6.1.2
TRANSCRIPTS
OF
ATNTRL
SHOW
A
REDUCED
LENGTH
IN
ATNTRL
MUTANTS
.
49
3.6.1.3
EFFECTS
OF
PEPTIDES
AS SOLE
SOURCE
OF
NITROGEN
ON
SEED
SET
OF
ATNTRL
PLANTS
.
50
3.6.1.4
UNDER
NITROGEN
STARVATION
ATNTRL
PLANTS
PRODUCE
SEEDS
WITH
DECREASED
VIABILITY.
52
4
DISCUSSION
.
55
4.1
THE
GENE
FAMILY
OF
PROTON-COUPLED
OLIGOPEPTIDE
TRANSPORTERS
(POT)
IN
PLANTS
.
55
4.1.1
ATNTR2,
A
PEPTIDE
TRANSPORTER
AND
NOVEL
MEMBER
OF
THE POT
FAMILY
.
56
4.1.1.1
PEPTIDE
TRANSPORTERS
OF
THE
POT
FAMILY
COULD
BE
INVOLVED
IN
MEDIATING
THE
EFFECTS
OF
PHASEOLOTOXIN
TOXICITY
.
57
4.1.2
CHARACTERISATION
OF
OTHER
MEMBERS
OF
THE
POT
FAMILY
.
58
4.1.3
VFPTRL,
A
DIPEPTIDE
TRANSPORTER
FROM
VICIAFABA
.
59
4.1.4
OTHER
POSSIBLE
FUNCTIONS
FOR
PEPTIDE
TRANSPORTERS
.
59
4.2
EXPRESSION
AND
REGULATION
OF
PEPTIDE
TRANSPORTER
GENES
.
60
4.2.1
ATNTR2
IS
EXPRESSED
IN
ALL
ORGANS
.
60
4.2.2
EXPRESSION
OF
P-GLUCURONIDASE
UNDER
CONTROL
OF
PROMOTERS
OF
POT
FAMILY
GENES
SHOWS
DIFFERENTIAL
REGULATION
.
60
4.2.2.1
ATNTRL
EXPRESSION
CORRELATES
TEMPORALLY
AND
SPATIALLY
WITH
STORAGE
PROTEIN
MOBILISATION
.
61
4.2.2.2
EXPRESSION
OF
ATNTR2
IN
THE
VASCULAR
TISSUE
MIGHT
INDICATE
INVOLVEMENT
IN
NITROGEN
REALLOCATION
.
'.
.
62
4.2.2.3
EXPRESSION
OF
OTHER
MEMBERS
OF
THE
POT
FAMILY
.
63
IN
TABLE
OF
CONTENTS
4.2.3
PROMOTER
ELEMENTS
ARE
AN
INDICATION
THAT
POT
GENES
ARE
REGULATED
SPECIFICALLY
DURING
GERMINATION,
SEED
LOADING
AND
SENESCENCE
.
63
4.2.4
EXPRESSION
OF
POT
FAMILY
MEMBERS
DURING
SENESCENCE
.
65
4.3
PHYSIOLOGICAL
FUNCTION
OF
PEPTIDE
TRANSPORTERS
.
65
4.3.1
ANALYSIS
OF
ATNTRL
PLANTS
.
66
4.3.1.1
ATNTRL
MIGHT
BE
INVOLVED
IN
REALLOCATION
OF
ORGANIC
NITROGEN
.
67
4.4
CONCLUSIONS
.
68
5
REFERENCES
.
70
6
APPENDICES
.
80
6.1
RESULTS
FROM
HYDROPHOBICITY
ANALYSIS
OF
ATNTR2
WITH
TMHMM
2.0
.
80
6.2
GENES
ANALYSED
BY
MACRO-ARRAY
HYBRIDISATION
.
81
6.3
ADDITIONAL
DATA
.
82
ACKNOWLEDGEMENTS
.
83
LEBENSLAUF
.
84
IV |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV016881797 |
| illustrated | Illustrated |
| indexdate | 2024-08-24T00:22:54Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010241572 |
| oclc_num | 260007854 |
| open_access_boolean | |
| owner | DE-12 DE-29T |
| owner_facet | DE-12 DE-29T |
| physical | IV, 85 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | <<D.>> Dietrich |
| record_format | marc |
| spelling | Dietrich, Daniela 1973- Verfasser (DE-588)124371043 aut Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana vorgelegt von Daniela Dietrich Heringen, Am guten Born 2 <<D.>> Dietrich 2002 IV, 85 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2002 Stickstoffstoffwechsel (DE-588)4138777-6 gnd rswk-swf Peptidtransporter (DE-588)4759852-9 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Peptidtransporter (DE-588)4759852-9 s Stickstoffstoffwechsel (DE-588)4138777-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010241572&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dietrich, Daniela 1973- Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana Stickstoffstoffwechsel (DE-588)4138777-6 gnd Peptidtransporter (DE-588)4759852-9 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4138777-6 (DE-588)4759852-9 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana |
| title_auth | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana |
| title_exact_search | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana |
| title_full | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana vorgelegt von Daniela Dietrich |
| title_fullStr | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana vorgelegt von Daniela Dietrich |
| title_full_unstemmed | Nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana vorgelegt von Daniela Dietrich |
| title_short | Nitrogen partitioning in plants |
| title_sort | nitrogen partitioning in plants the contribution of proton coupled oligopeptide transporters from arabidopsis thaliana |
| title_sub | the contribution of proton coupled oligopeptide transporters from Arabidopsis thaliana |
| topic | Stickstoffstoffwechsel (DE-588)4138777-6 gnd Peptidtransporter (DE-588)4759852-9 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Stickstoffstoffwechsel Peptidtransporter Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010241572&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dietrichdaniela nitrogenpartitioninginplantsthecontributionofprotoncoupledoligopeptidetransportersfromarabidopsisthaliana |