Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2003 |
| Beschreibung: | 84 Bl. Ill. |
Internformat
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| 245 | 1 | 0 | |a Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum |c von Hannes Wickert |
| 264 | 1 | |c 2003 | |
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Datensatz im Suchindex
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CONTENTS
1 GENERAL INTRODUCTION.7
1.1 THE MALARIAL PARASITE AND THE DISEASE.7
1.2 WHY IS THE INFECTION WITH
P. FALCIPARUM FATAL?.8
1.3 WHAT DOES SEQUESTRATION MEAN ON THE MOLECULAR LEVEL?.9
1.4 PFEMP 1, A HIGHLY DIVERS LIGAND.11
1.5 FROM
VAR GENES TO PFEMP 1 PROTEINS.11
1.6 TRAFFICKING OF PROTEINS IN THE CYTOPLASM OF EUKARYOTIC CELLS.12
1.7 REFERENCES.14
1.8 AIMS OF THE THESIS.19
2 EVIDENCE FOR TRAFFICKING OF PFEMPL TO THE SURFACE OF
P. FALCIPARUM-INFECTED
ERYTHROCYTES VIA A COMPLEX MEMBRANE NETWORK.20
2.1 ABSTRACT.20
2.2 INTRODUCTION.20
2.3 MATERIALS AND METHODS.22
2.3.1
P. FALCIPARUM CLONE AND CULTURE.22
2.3.2 ANTIBODIES.23
2.3.3 IMMUNOFLUORESCENCE MICROSCOPY.23
2.3.4 3D-RECONSTRUCTION OF SERIAL MICROGRAPHS.23
2.3.5 CONFOCAL MICROSCOPY AND 3D-RECONSTRUCTIONS.24
2.3.6 ELECTRON MICROSCOPY.24
2.3.7 POLYACRYLAMIDE GEL ELECTROPHORESIS.25
2.4 RESULTS.:.25
2.4.1 PFEMPL IS ASSOCIATED WITH AN EXTENDED POLYMORPHIC MEMBRANE NETWORK
25
2.4.2 A CONTINUOUS MEMBRANE SYSTEM IN THE INFECTED ERYTHROCYTE.29
2.4.3 SMALL VESICLES IN THE INFECTED ERYTHROCYTE.34
2.4.4 IN VIVO LABELING OF THE MEMBRANE NETWORK.34
2.5 DISCUSSION.36
2.6 REFERENCES.40
4
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/968395759
3 THE SEC23P HOMOLOGUE FROM
PLASMODIUM FALCIPARUM IS LOCATED
IN MAURER'S CLEFTS. 46
3.1 ABSTRACT.46
3.2 INTRODUCTION.46
3.3 MATERIALS, METHODS AND RESULTS.48
3.4 DISCUSSION.51
3.5 REFERENCES. 53
4 CHARACTERIZATION OF THE COPI PROTEIN PFSEC27 IN THE HUMAN MALARIA
PARASITE
PLASMODIUM FALCIPARUM
. 56
4.1 ABSTRACT.56
4.2 INTRODUCTION.57
4.3 MATERIALS AND METHODS.58
3.3.1
P. FALCIPARUM CULTURE.58
3.3.2 CLONING OF PLASMODIAL
SEC23 CDNA.58
3.3.3 NORTHERN BLOT ANALYSIS.59
3.3.4 PLASMID CONSTRUCTION FOR EXPRESSION OF HIS-TAG FUSION PROTEINS.59
3.3.5 INDIRECT IMMUNOFLUORESCENCE.60
4.4 RESULTS.60
4.4.1 IDENTIFCATION OF A SEC27/COPI HOMOLOGUE IN
P. FALCIPARUM.60
4.4.2 A-PFSEC27 RECOGNISES ONE POLYPEPTIDE BAND ON WESTERN BLOTS OF
INFECTED RED BLOOD CELLS.61
4.4.3 LOCALIZATION OF THE
P. FALCIPARUM SEC27 PROTEIN IN INFECTED ERYTHROCYTES. 61
4.5 DISCUSSION.68
4.6 REFERENCES.70
5 SUMMARIES.73
5.1 SUMMARY.73
5.2 ZUSAMMENFASSUNG.75
6 ACKNOWLEDGMENTS FOR MATERIALS AND DATA.77
5
7 ACKNOWLEDGEMENTS.
8 APPENDIX.79
8.1 ABBREVIATIONS.79
8.2 CURRICULUM VITAE.80
8.3 PUBLICATIONS.82
6 |
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| indexdate | 2024-11-22T17:36:16Z |
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| spelling | Wickert, Hannes 1965- Verfasser (DE-588)121271196 aut Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum von Hannes Wickert 2003 84 Bl. Ill. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2003 Proteintransport (DE-588)4273577-4 gnd rswk-swf Plasmodium falciparum (DE-588)4250521-5 gnd rswk-swf Erythrozyt (DE-588)4070945-0 gnd rswk-swf Mikroform (DE-588)4039216-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Plasmodium falciparum (DE-588)4250521-5 s Erythrozyt (DE-588)4070945-0 s Proteintransport (DE-588)4273577-4 s Mikroform (DE-588)4039216-8 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010233147&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Wickert, Hannes 1965- Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum Proteintransport (DE-588)4273577-4 gnd Plasmodium falciparum (DE-588)4250521-5 gnd Erythrozyt (DE-588)4070945-0 gnd Mikroform (DE-588)4039216-8 gnd |
| subject_GND | (DE-588)4273577-4 (DE-588)4250521-5 (DE-588)4070945-0 (DE-588)4039216-8 (DE-588)4113937-9 |
| title | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum |
| title_auth | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum |
| title_exact_search | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum |
| title_full | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum von Hannes Wickert |
| title_fullStr | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum von Hannes Wickert |
| title_full_unstemmed | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum von Hannes Wickert |
| title_short | Analysis of transport pathways in the red blood cell infected by the human malaria parasite P. falciparum |
| title_sort | analysis of transport pathways in the red blood cell infected by the human malaria parasite p falciparum |
| topic | Proteintransport (DE-588)4273577-4 gnd Plasmodium falciparum (DE-588)4250521-5 gnd Erythrozyt (DE-588)4070945-0 gnd Mikroform (DE-588)4039216-8 gnd |
| topic_facet | Proteintransport Plasmodium falciparum Erythrozyt Mikroform Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010233147&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wickerthannes analysisoftransportpathwaysintheredbloodcellinfectedbythehumanmalariaparasitepfalciparum |