Verfügbarkeit: Genetic transformation of plants

Genetic transformation of plants: with 11 tables

Gespeichert in:

Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	Berlin [u.a.] Springer 2003
Schriftenreihe:	Molecular methods of plant analysis 23
Schlagworte:	Cultures - Génie génétique Génie génétique végétal Transformation génétique végétale Crops > Genetic engineering Crops, Agricultural > genetics Genetic Engineering Plant genetic engineering Plant genetic transformation Plants, Genetically Modified > genetics Transformation, Genetic Pflanzenzüchtung Transformation > Genetik Gentechnologie Kulturpflanzen Pflanzen Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIX, 202 S. Ill., graph. Darst.
ISBN:	3540002928

Internformat

MARC


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245	1	0	\|a Genetic transformation of plants \|b with 11 tables \|c ed. by J. F. Jackson ...
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650		4	\|a Transformation génétique végétale
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650		4	\|a Crops, Agricultural \|x genetics
650		4	\|a Genetic Engineering
650		4	\|a Plant genetic engineering
650		4	\|a Plant genetic transformation
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Datensatz im Suchindex

_version_	1812898608318513152
adam_text	CONTENTS 1 EXCLUSIVE RIGHTS IN LIFE: BIOTECHNOLOGY, GENETIC MANIPULATION, AND INTELLECTUAL PROPERTY RIGHTS E.R. GOLD 1.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 BIOTECHNOLOGICAL INNOVATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2.1 PHYSICAL INNOVATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1.2.1.1 DNA AND PROTEIN MOLECULES . . . . . . . . . . . . . . . . . . 3 1.2.1.2 CELLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 1.2.1.3 WHOLE ORGANISMS . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.2.2 INFORMATION AND OTHER INTANGIBLES . . . . . . . . . . . . . . . . . . . . 4 1.2.2.1 DNA SEQUENCES AND CELLS . . . . . . . . . . . . . . . . . . . . 4 1.2.2.2 PROCESSES USING BIOLOGICAL MATTER . . . . . . . . . . . . . 5 1.2.2.3 BIOINFORMATICS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.3 SUMMARY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.3 INTRODUCTION TO INTELLECTUAL PROPERTY RIGHTS . . . . . . . . . . . . . . . . . . 6 1.3.1 EXCLUSIVE RIGHTS VS. RIGHTS TO THINGS . . . . . . . . . . . . . . . . . . 6 1.3.2 PROPERTY AND INTELLECTUAL PROPERTY RIGHTS . . . . . . . . . . . . . . 7 1.3.3 TRADE SECRETS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.3.3.1 SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.3.3.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.3.4 PATENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.3.4.1 SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.3.4.1.1 INVENTION VS. DISCOVERY . . . . . . . . . . . . . . 10 1.3.4.1.2 EXCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . 10 1.3.4.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.3.4.2.1 SUBSTANTIVE CRITERIA . . . . . . . . . . . . . . . . . 11 1.3.4.2.1.1 NOVELTY . . . . . . . . . . . . . . . . . . 12 1.3.4.2.1.2 INVENTIVE STEP (NONOBVIOUSNESS) . . . . . . . . . . 12 1.3.4.2.1.3 INDUSTRIAL APPLICATION (UTILITY) . . . . . . . . . . . . . . . . . . 12 1.3.4.2.2 PROCEDURAL CRITERION: DISCLOSURE . . . . . . . 13 1.3.4.3 REMEDIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 1.3.5 COPYRIGHT AND DATABASE PROTECTION . . . . . . . . . . . . . . . . . . . 14 1.3.5.1 SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 1.3.5.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 1.3.5.3 REMEDIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 1.3.6 PLANT VARIETY PROTECTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 1.3.6.1 SUBJECT MATTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 1.3.6.2 REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 1.4 CHALLENGES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 1.4.1 INCENTIVE VS. ACCESS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 1.4.1.1 JUSTIFICATION FOR PROPERTY RIGHTS . . . . . . . . . . . . . . . 18 1.4.1.2 ECONOMIC REALITY . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 1.4.2 FAIRNESS TO PROVIDERS OF BIOLOGICAL MATTER . . . . . . . . . . . . . . 20 1.4.2.1 RIGHTS TO BIOLOGICAL MATTER . . . . . . . . . . . . . . . . . . . 20 1.5 CONCLUSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 2 AGROBACTERIUM RHIZOGENES -MEDIATED TRANSFORMATION OF PLANTS W. VA N DEVELDE, M. KARIMI, G. DEN HERDER, M. VAN MONTAGU, M. HOLSTERS, AND S. GOORMACHTIG 2.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 2.2 ASPECTS INFLUENCING A. RHIZOGENES TRANSFORMATION EFFICIENCY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 2.2.1 CHOICE OF A. RHIZOGENES STRAIN . . . . . . . . . . . . . . . . . . . . . . . 26 2.2.2 CHOICE OF EXPLANT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 2.2.3 PREPARATION OF BACTERIAL INOCULUM AND INFECTION OF EXPLANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 2.2.4 CO-CULTIVATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 2.3 ESTABLISHING THE TRANSFORMED NATURE OF HAIRY ROOTS . . . . . . . . . . . 31 2.4 COTRANSFORMATION OF BINARY T-DNA . . . . . . . . . . . . . . . . . . . . . . . . . 31 2.5 PROPAGATION OF HAIRY ROOT LINES IN LIQUID CULTURES . . . . . . . . . . . . 33 2.5.1 THE CLONAL STATUS OF HAIRY ROOTS . . . . . . . . . . . . . . . . . . . . . 33 2.5.2 STABILITY OF LONG-TERM HAIRY ROOT CULTURES . . . . . . . . . . . . . 34 2.6 REGENERATION OF PLANTS FROM HAIRY ROOTS . . . . . . . . . . . . . . . . . . . . 34 2.7 THE MULTI-AUTO-TRANSFORMATION (MAT) VECTOR SYSTEM . . . . . . . . . . 35 2.8 CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 PROTOCOL 1: PRODUCTION OF TRANSFORMED HAIRY ROOTS . . . . . . . . . . . . . . . . 37 PROTOCOL 2: PLANT REGENERATION FROM HAIRY ROOTS . . . . . . . . . . . . . . . . . . 38 PROTOCOL 3: HAIRY ROOT LIQUID CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 3 TRANSFORMATION OF PETUNIA HYBRIDA BY THE AGROBACTERIUM SUSPENSION DROP METHOD S.J. WYLIE, D. TJOKROKUSUMO, AND J.A . MCCOMB 3.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.2 TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 3.3 ANALYSIS OF TRANSFORMANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 X CONTENTS 3.3.1 SCREENING PETUNIA SEEDLINGS FOR HERBICIDE RESISTANCE . . . . . 47 3.3.2 TRANSMISSION OF BASTA RESISTANCE PHENOTYPE TO T2 PROGENY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 3.3.3 B -GLUCURONIDASE ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 3.3.4 DNA ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 3.4 CONCLUSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4 ONION, LEEK AND GARLIC TRANSFORMATION BY CO-CULTIVATION WITH AGROBACTERIUM C.C. EADY 4.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 4.1.1 CURRENT APPLICATIONS OF ALLIUM TRANSFORMATION TECHNOLOGY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 4.1.1.1 PHYSIOLOGICAL STUDIES . . . . . . . . . . . . . . . . . . . . . . . . 53 4.1.1.2 HERBICIDE RESISTANCE . . . . . . . . . . . . . . . . . . . . . . . . 54 4.1.1.3 ANTIMICROBIAL RESISTANCE . . . . . . . . . . . . . . . . . . . . . 54 4.1.1.4 INSECT RESISTANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 4.2 ONION TRANSFORMATION PROTOCOLS . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 4.2.1 TRANSFORMATION USING ANTIBIOTIC AND VISUAL SELECTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.2.1.1 BACTERIAL STRAIN AND PLASMIDS . . . . . . . . . . . . . . . . . 56 4.2.1.2 TRANSFORMATION PROCEDURE (MODIFIED FROM EADY ET AL. 2000 . . . . . . . . . . . . . . . 56 4.2.2 TRANSFORMATION USING HERBICIDE SELECTION . . . . . . . . . . . . . . 57 4.2.2.1 BACTERIAL STRAIN AND PLASMIDS . . . . . . . . . . . . . . . . . 58 4.2.2.2 TRANSFORMATION PROCEDURE . . . . . . . . . . . . . . . . . . . 58 4.2.3 EX-FLASKING AND GROWTH IN CONTAINMENT . . . . . . . . . . . . . . . 58 4.2.4 TRANSGENE DETECTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 4.2.5 TRANSGENE EXPRESSION AND STABILITY . . . . . . . . . . . . . . . . . . . 60 4.2.5.1 VISUAL REPORTER GENES . . . . . . . . . . . . . . . . . . . . . . . 60 4.2.5.2 EXPRESSION OF HERBICIDE RESISTANCE . . . . . . . . . . . . 61 4.2.5.3 ANTISENSE ALLIINASE GENE EXPRESSION . . . . . . . . . . . 61 4.3 LEEK TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 4.4 GARLIC TRANSFORMATION PROTOCOL . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 4.4.1 BACTERIAL STRAIN AND PLASMIDS . . . . . . . . . . . . . . . . . . . . . . . . 63 4.4.2 TRANSFORMATION PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . 63 4.5 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 5 ELECTROPORATION TRANSFORMATION OF BARLEY F. GUEREL AND N. GOEZUEKIRMIZI 5.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 5.2 BACKGROUND OF ELECTROPORATION PROCEDURES . . . . . . . . . . . . . . . . . . . 71 CONTENTS XI 5.2.1 PRE- AND POST-ELECTROPORATION PERIOD . . . . . . . . . . . . . . . . . . 71 5.2.2 ELECTRICAL VARIABLES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 5.3 CULTURE AND ELECTROPORATION OF BARLEY EXPLANTS . . . . . . . . . . . . . . . 74 5.3.1 PROTOPLASTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 5.3.2 MICROSPORES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 5.3.3 INTACT TISSUES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 5.3.3.1 ANALYSIS AND INHERITANCE OF TRANSGENES IN ELECTROPORATED TISSUES . . . . . . . . . . . . . . . . . . . . 81 5.4 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . . . . . . . . . . . . . . . . . . . 83 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 6 SORGHUM TRANSFORMATION Z. ZHAO AND D. TOMES 6.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 6.2 SORGHUM TRANSFORMATION PROCESS AND OPTIMIZATION . . . . . . . . . . . 92 6.2.1 PLANT MATERIALS AND TRANSFORMATION SYSTEMS . . . . . . . . . . . . 92 6.2.2 TRANSFORMATION VIA MICROPROJECTILE BOMBARDMENT . . . . . . . 93 6.2.3 AGROBACTERIUM -MEDIATED TRANSFORMATION . . . . . . . . . . . . . . . 94 6.3 ANALYSIS OF TRANSGENIC PLANTS AND THE PROGENY . . . . . . . . . . . . . . . . 96 6.3.1 MOLECULAR ANALYSIS OF T0 PLANTS . . . . . . . . . . . . . . . . . . . . . . 97 6.3.2 FOREIGN GENE EXPRESSION IN T0 PLANTS . . . . . . . . . . . . . . . . . . 98 6.3.3 GENETIC AND MOLECULAR ANALYSIS OF THE PROGENY . . . . . . . . . . 99 6.4 MARKER-FREE SORGHUM TRANSGENIC PLANTS . . . . . . . . . . . . . . . . . . . . . 99 6.4.1 IMPORTANCE OF MARKER-FREE TRANSGENICS IN SORGHUM . . . . . . 100 6.4.2 METHODS TO ELIMINATE MARKERS FROM TRANSGENIC PLANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 6.4.3 AGROBACTERIUM 2 T-DNA CO-TRANSFORMATION SYSTEM . . . . . . 101 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 7 TRANSGENIC SUNFLOWER: PEG-MEDIATED GENE TRANSFER P.C. BINSFELD 7.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 7.2 GENETIC VARIABILITY AND TRANSGENIC BREEDING . . . . . . . . . . . . . . . . . . 109 7.3 GENE TRANSFER SYSTEMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 7.3.1 PEG-MEDIATED GENE TRANSFER . . . . . . . . . . . . . . . . . . . . . . . . 111 7.3.1.1 SHORT DNA MOLECULE UPTAKE . . . . . . . . . . . . . . . . . . 111 7.3.1.2 LARGE DNA MOLECULE UPTAKE . . . . . . . . . . . . . . . . . . 112 7.4 PLANT REGENERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 7.5 GENERAL ANALYTICAL CONSIDERATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . 115 7.5.1 MOLECULAR ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 7.5.1.1 DNA EXTRACTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116 7.5.1.2 SOUTHERN HYBRIDIZATION . . . . . . . . . . . . . . . . . . . . . . 116 7.5.1.3 POLYMERASE CHAIN REACTION . . . . . . . . . . . . . . . . . . 116 7.5.1.4 RANDOM AMPLIFIED POLYMORPHIC DNA . . . . . . . . . . 117 XII CONTENTS 7.5.2 BIOCHEMICAL ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118 7.5.2.1 MULTIPLE MOLECULAR FORMS OF ENZYMES . . . . . . . . . . 118 7.5.2.2 ENZYMATIC ASSAY . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 7.5.3 CYTOGENETIC ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 7.5.3.1 FLOW CYTOMETRIC ANALYSIS . . . . . . . . . . . . . . . . . . . . 120 7.5.3.2 MITOTIC AND MEIOTIC CELL ANALYSIS . . . . . . . . . . . . . . 122 7.5.3.3 IN SITU HYBRIDIZATION . . . . . . . . . . . . . . . . . . . . . . . . 122 7.5.4 MORPHOLOGICAL ANALYSIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 7.6 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . . . . . . . . . . . . . . . . . . . 124 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124 8 TRANSFORMATION OF NORWAY SPRUCE ( PICEA ABIES ) BY PARTICLE BOMBARDMENT D.H. CLAPHAM, H. HAEGGMAN, M. ELFSTRAND, T. ARONEN, AND S. VON ARNOLD 8.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 8.2 TYPES OF PARTICLE ACCELERATOR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 8.3 TRANSFORMATION OF EMBRYOGENIC CULTURES . . . . . . . . . . . . . . . . . . . . 128 8.3.1 TRANSIENT EXPRESSION IN EMBRYOGENIC CULTURES . . . . . . . . . . 128 8.3.2 PRODUCTION OF STABLY TRANSFORMED CELL CULTURES AND TRANSGENIC PLANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129 8.3.3 STABILITY OF TRANSGENE EXPRESSION . . . . . . . . . . . . . . . . . . . . . 131 8.3.4 TRENDS IN TRANSGENIC PLANT PRODUCTION . . . . . . . . . . . . . . . . . 131 8.4 TRANSFORMATION OF POLLEN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 8.4.1 THE REPRODUCTIVE BIOLOGY OF NORWAY SPRUCE . . . . . . . . . . . . 133 8.4.2 TRANSIENT EXPRESSION IN POLLEN . . . . . . . . . . . . . . . . . . . . . . . 134 8.4.3 DEVELOPMENT OF CONTROLLED POLLINATION TECHNIQUES FOR BOMBARDED POLLEN . . . . . . . . . . . . . . . . . . . . 135 8.5 APPLICATIONS OF TRANSGENIC NORWAY SPRUCE IN RESEARCH . . . . . . . . . 136 8.5.1 GENES REGULATING EMBRYOGENESIS . . . . . . . . . . . . . . . . . . . . . 136 8.5.2 GENES WITH SIMILARITY TO DEFENSE GENES . . . . . . . . . . . . . . . . 137 8.6 PROSPECTS FOR TRANSGENIC NORWAY SPRUCE IN PRACTICAL FORESTRY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 9 WHISKERS-MEDIATED TRANSFORMATION OF MAIZE J.F. PETOLINO, M. WELTER, AND C. QIHUA CAI 9.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147 9.2 PREPARATION OF PURIFIED DNA FRAGMENTS . . . . . . . . . . . . . . . . . . . . . 147 9.3 ESTABLISHMENT AND MAINTENANCE OF EMBRYOGENIC SUSPENSION CULTURES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 9.4 DNA DELIVERY VIA WHISKERS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 9.5 TRANSGENE COPY NUMBER ESTIMATION . . . . . . . . . . . . . . . . . . . . . . . . 153 9.6 REGENERATION OF TRANSGENIC PLANTS AND PROGENY . . . . . . . . . . . . . . . 157 CONTENTS XIII XIV CONTENTS 9.7 CONCLUSIONS AND FUTURE PERSPECTIVES . . . . . . . . . . . . . . . . . . . . . . . . 157 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 10 GENETIC TRANSFORMATION OF SOYBEAN WITH BIOLISTICS D. SIMMONDS 10.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 10.2 TISSUE CULTURE AND PLANT REGENERATION . . . . . . . . . . . . . . . . . . . . . . 160 10.2.1 GENOTYPE SPECIFICITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160 10.2.2 INITIATION AND REPETITIVE PROLIFERATION OF SOMATIC EMBRYOGENIC CULTURES . . . . . . . . . . . . . . . . . . . . . 161 10.2.3 EMBRYO HISTODIFFERENTIATION AND MATURATION . . . . . . . . . . . . 163 10.2.4 GERMINATION, CONVERSION AND PLANT FERTILITY . . . . . . . . . . . . 163 10.3 TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164 10.3.1 GENE DELIVERY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164 10.3.2 TARGET TISSUE OPTIMIZATION . . . . . . . . . . . . . . . . . . . . . . . . . . 165 10.3.3 SELECTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165 10.3.4 TRANSGENIC PLANT RECOVERY . . . . . . . . . . . . . . . . . . . . . . . . . . 166 10.4 CONCLUSIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167 10.5 PROTOCOL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 10.5.1 INDUCTION AND MAINTENANCE OF PROLIFERATIVE EMBRYOGENIC CULTURES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 10.5.2 TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 10.5.3 SELECTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169 10.5.4 PLANT REGENERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 11 GENOTOXIC EFFECTS OF TUNGSTEN MICROPARTICLES UNDER CONDITIONS OF BIOLISTIC TRANSFORMATION J. BUCHOWICZ AND C. KRYSIAK 11.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 11.2 BIOLOGICAL SIGNIFICANCE OF TUNGSTEN . . . . . . . . . . . . . . . . . . . . . . . . . 175 11.2.1 EARLY OBSERVATIONS ON BIOLOGICAL EFFECTS OF TUNGSTEN . . . . . 175 11.2.2 CATALYTIC ACTIVITY OF SIMPLE TUNGSTEN COMPOUNDS . . . . . . . 176 11.2.3 TUNGSTOENZYMES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 11.2.4 TUNGSTEN*DNA INTERACTION . . . . . . . . . . . . . . . . . . . . . . . . . . 177 11.3 TUNGSTEN MICROPARTICLES IN BIOTECHNOLOGICAL APPLICATIONS . . . . . . . 178 11.3.1 BIOLISTIC TRANSFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178 11.3.1.1 AN OVERVIEW . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178 11.3.1.2 TECHNICAL DETAILS . . . . . . . . . . . . . . . . . . . . . . . . . . . 180 11.3.2 BIOLISTIC INOCULATION AND RELATED APPLICATIONS OF TUNGSTEN PARTICLES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181 11.4 ASSESSMENT OF TUNGSTEN-INDUCED DNA LESIONS . . . . . . . . . . . . . . . . 182 11.4.1 ELECTROPHORETIC ANALYSIS OF TUNGSTEN-DAMAGED PLASMID DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182 11.4.2 A MODIFIED TUNEL METHOD FOR DETECTION OF CELLULAR DNA FRAGMENTATION . . . . . . . . . . . . . . . . . . . . . . 184 11.5 POST-BOMBARDMENT INHIBITION OF SOMATIC EMBRYOGENESIS . . . . . . . 186 11.6 CONCLUDING REMARKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188 REFERENCES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188 SUBJECT INDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195 CONTENTS XV
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discipline	Biologie Agrarwissenschaft Chemie-Ingenieurwesen Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Biotechnologie Pflanzenbau
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series	Molecular methods of plant analysis
series2	Molecular methods of plant analysis
spelling	Genetic transformation of plants with 11 tables ed. by J. F. Jackson ... Berlin [u.a.] Springer 2003 XIX, 202 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Molecular methods of plant analysis 23 Cultures - Génie génétique Génie génétique végétal Transformation génétique végétale Crops Genetic engineering Crops, Agricultural genetics Genetic Engineering Plant genetic engineering Plant genetic transformation Plants, Genetically Modified genetics Transformation, Genetic Pflanzenzüchtung (DE-588)4045599-3 gnd rswk-swf Transformation Genetik (DE-588)4268828-0 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Kulturpflanzen (DE-588)4033576-8 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Transformation Genetik (DE-588)4268828-0 s DE-604 Kulturpflanzen (DE-588)4033576-8 s Gentechnologie (DE-588)4071722-7 s Pflanzenzüchtung (DE-588)4045599-3 s Jackson, John F. 1935- Sonstige (DE-588)131937375 oth Molecular methods of plant analysis 23 (DE-604)BV035419634 23 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010211633&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Genetic transformation of plants with 11 tables Molecular methods of plant analysis Cultures - Génie génétique Génie génétique végétal Transformation génétique végétale Crops Genetic engineering Crops, Agricultural genetics Genetic Engineering Plant genetic engineering Plant genetic transformation Plants, Genetically Modified genetics Transformation, Genetic Pflanzenzüchtung (DE-588)4045599-3 gnd Transformation Genetik (DE-588)4268828-0 gnd Gentechnologie (DE-588)4071722-7 gnd Kulturpflanzen (DE-588)4033576-8 gnd Pflanzen (DE-588)4045539-7 gnd
subject_GND	(DE-588)4045599-3 (DE-588)4268828-0 (DE-588)4071722-7 (DE-588)4033576-8 (DE-588)4045539-7 (DE-588)4143413-4
title	Genetic transformation of plants with 11 tables
title_auth	Genetic transformation of plants with 11 tables
title_exact_search	Genetic transformation of plants with 11 tables
title_full	Genetic transformation of plants with 11 tables ed. by J. F. Jackson ...
title_fullStr	Genetic transformation of plants with 11 tables ed. by J. F. Jackson ...
title_full_unstemmed	Genetic transformation of plants with 11 tables ed. by J. F. Jackson ...
title_short	Genetic transformation of plants
title_sort	genetic transformation of plants with 11 tables
title_sub	with 11 tables
topic	Cultures - Génie génétique Génie génétique végétal Transformation génétique végétale Crops Genetic engineering Crops, Agricultural genetics Genetic Engineering Plant genetic engineering Plant genetic transformation Plants, Genetically Modified genetics Transformation, Genetic Pflanzenzüchtung (DE-588)4045599-3 gnd Transformation Genetik (DE-588)4268828-0 gnd Gentechnologie (DE-588)4071722-7 gnd Kulturpflanzen (DE-588)4033576-8 gnd Pflanzen (DE-588)4045539-7 gnd
topic_facet	Cultures - Génie génétique Génie génétique végétal Transformation génétique végétale Crops Genetic engineering Crops, Agricultural genetics Genetic Engineering Plant genetic engineering Plant genetic transformation Plants, Genetically Modified genetics Transformation, Genetic Pflanzenzüchtung Transformation Genetik Gentechnologie Kulturpflanzen Pflanzen Aufsatzsammlung
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010211633&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV035419634
work_keys_str_mv	AT jacksonjohnf genetictransformationofplantswith11tables

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