Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Bonn, Univ., Diss., 2002 |
| Beschreibung: | 165 S.: Ill., graph. Darst. 21 cm |
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Datensatz im Suchindex
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ISOLATION AND EVALUATION OF BIOLOGICALLY ACTIVE SECONDARY METABOLITES
FROM SELECTED MEDICINAL PLANTS OF SUDAN DISSERTATION ZUR ERLANGUNG DES
DOKTORGRADES (DR. RER. NAT.) DER MATHEMATISCH-NATURWISSENSCHAFTLICHEN
FAKULTAT DER RHEINISCHEN FRIEDRICH-WILHELMS-UNIVERSITAT BONN VORGELEGT
VON HIBA ABDEL-RAHMAN AH AUS KHARTOUM - SUDAN BONN 2002 TABLE OF
CONTENTS TABLE OF CONTENTS PAGE 1 INTRODUCTION 1 1 .1 TROPICAL DISEASES
WITH SPECIAL REFERENCE TO AFRICA 1 1.2 TRADITIONAL MEDICINE 2 1.2.1
ETHNOPHARMACOLOGY IN SUDAN 3 1.3 IMPORTANCE OF HIGHER PLANTS IN DRUG
DISCOVERY 6 1.4 AMARYLLIDACEAE SECONDARY METABOLITES AND THEIR 12
PHARMACOLOGICAL SIGNIFICANCE 1.5 COMBRETUM (COMBRETACEAE) SECONDARY
METABOLITES 17 AND THEIR PHARMACOLOGICAL SIGNIFICANCE 2 OBJECTIVES OF
THIS STUDY 23 2.1 BIOLOGICAL SCREENING OF PLANTS STUDIED 23 2.2 CHEMICAL
INVESTIGATION OF SELECTED ACTIVE PLANTS 23 2.3 BIOLOGICAL TESTING OF
ISOLATED COMPOUNDS 24 2.4 ESTABLISHMENT OF AN ANTIMALARIAL ASSAY USING
HUMAN 24 GLUTATHIONE REDUCTASE (GR) AS TARGET 3 MATERIALS AND METHODS
(GENERAL PHYTOCHEMICAL METHODS) 25 25 25 25 25 26 26 27 27 28 28 29 29
29 29 29 30 31 31 32 33 3.1 PLANT MATERIAL COLLECTION 3.1.1 PLANT
MATERIAL PREPARATION AND EXTRACTION 3.2 CHROMATOGRAPHY 3.2.1 3.: 3.2.2
3.2.3 THIN LAYER CHROMATOGRAPHY 2.1.1 SPRAY REAGENTS VACUUM-LIQUID
CHROMATOGRAPHY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 3.3 STRUCTURE
ELUCIDATION 3.3.1 3.3.2 3.3.3 3.3.4 ' 3.3.5 NMR SPECTROSCOPY MASS
SPECTROMETRY 1R SPECTROSCOPY UV MEASUREMENTS OPTICAL ROTATIONS 3.4
BIOLOGICAL TESTING 3.4.1 3.4.2 3.4.3 3.4.4 3.4.5 3.4.6 AGAR DIFFUSION
ASSAYS TYROSINE KINASE INHIBITORY ACTIVITY H1V-1 REVERSE TRANSCRIPTASE
INHIBITORY ACTIVITY SCREENING FOR ANTIPLASMODIAL ACTIVITY SCREENING FOR
ANTITRYPANOSOMAL ACTIVITY SCREENING FOR ANTILEISHMANIAL ACTIVITY TABLE
OF CONTENTS 3.4.7 CYTOTOXICITY ACTIVITY 33 3.5 CHEMICALS 34 4 EVALUATION
OF SELECTED SUDANESE MEDICINAL PLANTS FOR THEIR IN VITRO 35
ANTIPARASITIC, HIV- 1-RT AND TYROSINE KINASE INHIBITORY, AND
CYTOTOXICITY 35 4.1 INTRODUCTION 36 4.2 MATERIALS AND METHODS 36 4.3
RESULTS 36 4.4 DISCUSSION AND CONCLUSION 43 5 PHYTOCHEMICAL
INVESTIGATION OF PAMIANTHEPERUVIANA STAPF. 47 (AMARYLLIDACEAE) 5.1
INTRODUCTION 47 5.2 PLANT MATERIAL PREPARATION AND EXTRACTION 47 5.2.1
LIQUID-LIQUID PARTITIONING FOR ALKALOIDS 49 5.3 RESULTS AND DISCUSSION
50 5.3.1 BIOLOGICAL ACTIVITY GUIDED FRACTIONATION AND ISOLATION 50 5.3.2
ISOLATION OF COMPOUND 1 55 5.3.2.1 CHARACTERISATION OF COMPOUND 1 AS
GLYCOLIPID 55 5.3.3 ISOLATION OF COMPOUND 2 58 5.3.1.1 CHARACTERISATION
OF COMPOUND 2 AS EPIHAEMANTHIDINE 59 5.3.4 ISOLATION OF COMPOUND 3 63
5.3.4.1 CHARACTERISATION OF COMPOUND 3 AS HIPPADINE 64 5.3.5 ISOLATION
OF COMPOUND 4 69 5.3.5.1 CHARACTERIZATION OF COMPOUND 4 AS LYCORINE 70
5.3.6 ISOLATION OF COMPOUND 5 74 5.3.6.1 CHARACTERISATION OF COMPOUNDS
75 5.3.7 ISOLATION OF COMPOUND 6 78 5.3.7.1 CHARACTERISATION OF COMPOUND
6 AS 3,4-DIHYDROXYPHENYL P-GLUCOPYRANOSIDE 79 5.3.8 ISOLATION AND
CHARACTERISATION OF DIFFERENT STEROLS FROM P. PERUVIANA 81 5.4
CONCLUSION 84 6 PHYTOCHEMICAL INVESTIGATION OF COMBRETUM HARTMANNIANUM
SCHWEINF. 87 (COMBRETACEAE) T 6.1 INTRODUCTION 87 6.2 MATERIALS AND
METHODS 87 6.2.1 PLANT MATERIAL PREPARATION AND EXTRACTION 87 6.2.2
REMOVING PLANT POLYPHENOLS 89 6.3 RESULTS AND DISCUSSION 89 6.3.1
BIOLOGICAL ACTIVITY GUIDED FRACTIONATION OF C. HARTMANNIANUM 89 6.3.2
ENZYME INHIBITORY ACTIVITIES OF C HARTMANNIANUM FRACTIONS AFTER
TREATMENT 90 WITHL%NACI TABLE OF CONTENTS 6.3.3 PRELIMINARY
PHYTOCHEMICAL CHARACTERISATION OF ISOLATED COMPOUNDS 91 6.4 CONCLUSION
98 7 OVEREXPRESSION AND ISOLATION OF HUMAN GLUTATHIONE REDUCTASE (GR)
101 A TARGET MOLECULE FOR ANTIMALARIAL ACTIVITY 7.1 INTRODUCTION 101
7.1.1 GLUTATHIONE 101 7.1.2 GLUTATHIONE REDUCTASE (GR) 101 7.1.2.1
OCCURANCE OF GLUTATHIONE REDUCTASE (GR) 103 7.1.2.2 FUNCTIONS OF
GLUTATHIONE REDUCTASE (GR) 103 7.1.2.3 THREE DIMENTIONAL STRUCTURE OF
HUMAN GLUTATHIONE REDUCTASE (HGR) 104 7.2.3 WHY IS HUMAN GR AN
INTERESTING DRUG TARGET? 105 7.2.4 GLUTATHIONE REDUCTASE AS AN
ANTIMALARIAL TARGET 106 7.2.5 INHIBITORS OF GLUTATHIONE REDUCTASE (GR)
107 7.2 MATERIALS AND METHODS 111 7.2.1 CHEMICALS, ENZYMES AND OTHER
MATERIALS 111 7.2.1.1 CHEMICALS 111 7.2.1.2 ENZYMES 112 7.2.1.3
COMMERCIAL KITS 112 7.2.1.4 OTHER MATERIALS 112 7.2.2 MOLECULAR WEIGHT
MARKERS 113 7.2.3 ESCHERICHIA COLI STRAINS (COMPETENT CELLS) 113 7.2.4
PLASMIDS 114 7.2.5 PRIMERS 114 7.2.6 MEDIA 115 7.2.7 BUFFERS AND
SOLUTIONS 115 7.2.8 STERILIZATION 118 7.2.9 CULTIVATION AND CONSERVATION
OFE. COLI FOR HETEROGENOUS PROTEIN EXPRESSION 118 7.2.10 MOLECULAR
BIOLOGICAL METHODS 119 7.2.10.1 PREPARATION OF PLASMIDS 119 7.2.10.2
PRECIPITATION OF DNA 119 7.2.10.3 DNA GEL ELECTROPHORESIS 120 7.2.10.4
AMPLIFICATION OF DNA USING POLYMERASE CHAIN 121 REACTION (PCR) 7.2.10.5
RESTRICTION ENZYMES DIGESTS 122 7.2.10.6 SUBCLONING 122 7.2.10.7
PREPARATION OFF. COLI COMPETENT CELLS 123 7.2.10.8 TRANSFORMATION OF
COMPETENT CELLS 123 7.2.11 PROTEIN EXPRESSION AND CHEMICAL METHODS 124
TABLE OF CONTENTS 7.2.11.1 CLONING OF RECOMBINANT PLASMID INTO 125
OVER-EXPRESSION VECTOR PQE 30 7.2.11.2 OVER-EXPRESSION OF HUMAN
GLUTATHIONE REDUCTASE 126 IN E. COLI STRAINS 7.2.11.3 DETERMINATION OF
THE TARGET PROTEIN SOLUBILITY 126 UNDER NATIVE CONDITIONS 7.2.11.3
PURIFICATION OF THE 6XHIS TAGGED PROTEIN 128 7.2.12 POLYACRYLAMID GEL
ELECTROPHORESIS 128 7.3 RESULTS AND DISCUSSION 129 7.3.1 PREPARATION OF
PCR 2.1-HGR SUBCLONE 129 7.3.2 CONSTRUCTION OF A RECOMBINANT PLASMID
(PQE 30-HAP17-HGR) 131 FOR EXPRESSION OF HUMAN GR (HGR) 7.3.3 ANALYSIS
OF THE EXPRESSION OF HUMAN GR INF. COLI M15[PREP4] 131 AND
SG13009[PREP4] 7.3.4 PURIFICATION OF HGR FROM E. COLI M15[PREP4] AND
SG13009[PREP4] 1 34 7.4 CONCLUSIONS 135 8. GENERAL SUMMARY AND FUTURE
PROSPECTS 137 8.1 ETHNOPHARMACOLOGICAL ASPECTS 137 8.2 PHYTOCHEMICAL
ASPECTS 138 8.3 OVER-EXPRESSION OF GLUTATHIONE REDUCTASE 140 9.
REFERENCES 143 10. APPENDIX 153 |
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| indexdate | 2024-08-10T01:41:33Z |
| institution | BVB |
| language | English |
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| spelling | Ali, Hiba Abdel-Rahman 1966- Verfasser (DE-588)124353851 aut Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan Hiba Abdel-Rahman Ali 2002 165 S.: Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Bonn, Univ., Diss., 2002 Heilpflanzen (DE-588)4024076-9 gnd rswk-swf Sekundärmetabolit (DE-588)4300564-0 gnd rswk-swf Bioaktive Verbindungen (DE-588)4145589-7 gnd rswk-swf Sudan (DE-588)4058378-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Sudan (DE-588)4058378-8 g Heilpflanzen (DE-588)4024076-9 s Sekundärmetabolit (DE-588)4300564-0 s Bioaktive Verbindungen (DE-588)4145589-7 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010204171&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ali, Hiba Abdel-Rahman 1966- Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan Heilpflanzen (DE-588)4024076-9 gnd Sekundärmetabolit (DE-588)4300564-0 gnd Bioaktive Verbindungen (DE-588)4145589-7 gnd |
| subject_GND | (DE-588)4024076-9 (DE-588)4300564-0 (DE-588)4145589-7 (DE-588)4058378-8 (DE-588)4113937-9 |
| title | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan |
| title_auth | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan |
| title_exact_search | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan |
| title_full | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan Hiba Abdel-Rahman Ali |
| title_fullStr | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan Hiba Abdel-Rahman Ali |
| title_full_unstemmed | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan Hiba Abdel-Rahman Ali |
| title_short | Isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of Sudan |
| title_sort | isolation and evaluation of biologically active secondary metabolites from selected medicinal plants of sudan |
| topic | Heilpflanzen (DE-588)4024076-9 gnd Sekundärmetabolit (DE-588)4300564-0 gnd Bioaktive Verbindungen (DE-588)4145589-7 gnd |
| topic_facet | Heilpflanzen Sekundärmetabolit Bioaktive Verbindungen Sudan Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010204171&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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