Microscopic techniques in biotechnology:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XII, 330 S. Ill., graph. Darst. : 25 cm |
| ISBN: | 3527301984 |
Internformat
MARC
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| 100 | 1 | |a Hoppert, Michael |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Microscopic techniques in biotechnology |c Michael Hoppert |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2003 | |
| 300 | |a XII, 330 S. |b Ill., graph. Darst. : 25 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturangaben | ||
| 650 | 4 | |a Biotechnologie | |
| 650 | 4 | |a Microscopie | |
| 650 | 4 | |a Biotechnology | |
| 650 | 4 | |a Microscopy | |
| 650 | 4 | |a Microscopy |x methods | |
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroskopie |0 (DE-588)4039238-7 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
| 689 | 0 | 1 | |a Mikroskopie |0 (DE-588)4039238-7 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010171132 | |
Datensatz im Suchindex
| _version_ | 1808135727654895616 |
|---|---|
| adam_text |
IX
CONTENTS
PREFACE
V
ACKNOWLEDGEMENTS
VI
1
LIGHT
MICROSCOPY
1
1.1
APPLICATIONS
AND
LIMITATIONS
IN
LIGHT
MICROSCOPY
1
1.2
USEFUL
APPLICATIONS
2
1.2.1
CELLULAR
AND
TISSUE
STRUCTURE
2
1.2.1.1
PROKARYOTIC
CELL
STRUCTURES
AT
THE
LIGHT
MICROSCOPIC
LEVEL
2
1.2.1.2
EUKARYOTIC
CELL
STRUCTURES
AT
THE
LIGHT
MICROSCOPIC
LEVEL
6
1.2.2
MONITORING
OF
INTERACTIONS
OF
ORGANISMS
26
1.2.2.1
BIOFILMS
AND
BIOFOULING
26
1.2.2.2
PLANT-MICROBE
INTERACTIONS:
SYMBIOSIS
37
1.2.2.3
ANIMAL-MICROBE
INTERACTIONS
48
1.3
APPLICATIONS
AND
LIMITATIONS
OF
CONFOCAL
LASER
SCANNING
MICROSCOPES
(CLSMS)
62
1.3.1
IMAGING
OF
CELIS
AND
CELLULAR
COMPONENTS
63
1.3.2
IMAGING
OF
COMPLEX
SPECIMENS
64
1.3.2.1
THREE-DIMENSIONAL
IMAGING
OF
MICROBIAL
COMMUNITIES
66
1.4
INSTRUMENTATION
AND
PREPARATION
TECHNIQUES
71
1.4.1
BASIC
FEATURES
OF
LENSES,
LENS
ABERRATION,
AND
CORRECTION
71
1.4.1.1
SPHERICAL
ABERRATION
72
1.4.1.2
COMATIC
ABERRATIONS
72
1.4.1.3
ASTIGMATISM
72
1.4.1.4
CURVATURE
OF
FIELD
73
1.4.1.5
GEOMETRICAL
DISTORTION
73
1.4.1.6
CHROMATIC
ABERRATIONS
73
1.4.1.7
NUMERICAL
APERTURE
AND
RESOLUTION
73
1.4.2
MICROSCOPE
PARTS
75
1.4.3
STANDARD
FEATURES
FOR
ENHANCEMENT
AND
MODIFICATION
OF
IMAGE
CONTRAST
IN
LIGHT
MICROSCOPES
80
1.4.3.1
PHASE
CONTRAST
(PLUTA,
1969)
80
1.4.3.2
DARK-FIELD
MICROSCOPY
82
X
CONTENTS
1.4.3.3
1.4.3.4
1.4.3.5
1.4.4
1.4.5
1.4.5.1
1.4.5.2
1.4.6
1.4.7
1.4.8
1.4.8.1
1.4.9
1.4.10
1.4.11
1.4.11.1
1.4.11.2
1.4.11.3
1.4.11.4
1.4.12
1.4.12.1
1.4.13
1.4.14
1.4.14.1
1.4.14.2
1.4.14.3
POLARIZING
MICROSCOPY
82
DIFFERENTIAL
INTERFERENCE
CONTRAST
84
HOFFMAN
MODULATION
CONTRAST
85
UPRIGHT
AND
INVERTED
MICROSCOPES,
EPI-ILLUMINATION
MICROSCOPES
85
FLUORESCENCE
MICROSCOPY
86
FLUORESCENCE
MICROSCOPES
87
CONFOCAL
MICROSCOPES
88
STEREO
MICROSCOPY
92
IMAGE
ACQUISITION
AND
PROCESSING
93
VISUALIZATION
OF
LIVING
UNICELLULAR
ORGANISMS
96
BRIGHT-FIELD
STAINING
TECHNIQUES
FOR
UNICELLULAR
ORGANISMS
97
EMBEDDING
AND
SECTIONING
-
A
QUICK
START
100
TISSUE
SECTIONING
WITH
VIBRATOMES
101
FIXATION,
EMBEDDING,
AND
SECTIONING
OF
SPECIMENS
102
FIXATION
103
DEHYDRATION
AND
EMBEDDING
107
THIN
SECTIONING
110
SPECIMEN
MOUNTING
112
CRYOTECHNIQUES
IN
LIGHT
MICROSCOPY
114
FREEZE
SECTIONING
115
STAINING
OF
SECTIONS
117
PREPARATION
OF
SPECIMENS
FOR
FLUORESCENCE
MICROSCOPY
117
FLUORESCENT
DYES
120
FLUORESCENT
PROBES
126
IMMUNOFLUORESCENCE
TECHNIQUES
128
2
2.1
2.2
2.2.1
2.2.1.1
2.2.1.2
2.2.1.3
2.2.2
2.2.3
2.2.3.1
2.2.4
2.2.5
2.2.6
2.2.7
2.2.8
2.2.9
2.3
2.3.1
ELECTRON
MICROSCOPY
147
APPLICATIONS
AND
LIMITATIONS
IN
TRANSMISSION
ELECTRON
MICROSCOPY
147
IMAGING
OF
CELLULAR
AND
LARGE
SUBCELLULAR
STRUCTURES
148
BACTERIAL
CELIS
148
OUTSIDE
THE
CYTOPLASMIC
MEMBRANE
148
COMPARTMENTATION
OF
THE
BACTERIAL
NUCLEOID
151
INCLUSION
BODIES
153
PLANT
CELIS
155
ANIMAL
CELIS
164
STRUCTURAL
ASPECTS
OF
THE
ENDOCYTIC
AND
EXOCYTOTIC
PATHWAYS
168
INTERACTION
BETWEEN
CELIS
IN
ANIMAL
TISSUE
169
ENZYME
COMPLEXES
IN
VIVO
AND
IN
VITRO
171
INTERACTION
OF
ORGANISMS
178
SPECIFIC
MARKERS
FOR
INTRACELLULAR
COMPONENTS
182
COMPARTMENTATION
OF
BIOCHEMICAL
PATHWAYS
184
ELEMENTAL
ANALYSIS
186
APPLICATIONS
AND
LIMITATIONS
IN
SCANNING
ELECTRON
MICROSCOPY
191
VISUALIZATION
OF
SURFACE
AND
INTERNAL
CELLULAR
STRUCTURES
192
CONTENTS
I
XI
2.3.2
INTERACTIONS
BETWEEN
ORGANISMS
194
2.3.3
SELECTIVE
DETECTION
OF
SURFACE
STRUCTURES
200
2.4
PREPARATION
TECHNIQUES
201
2.4.1
THE
TRANSMISSION
ELECTRON
MICROSCOPE
201
2.4.1.1
IMAGE
CONTRAST
FORMATION
IN
THE
TRANSMISSION
ELECTRON
MICROSCOPE
204
2.4.1.2
ANALYTICAL
FEATURES
IN
TRANSMISSION
ELECTRON
MICROSCOPES
206
2.4.2
SCANNING
ELECTRON
MICROSCOPY
208
2.4.3
SPECIMEN
SUPPORT
210
2.4.3.1
GRIDS
210
2.4.3.2
PLASTIC
SUPPORT
FILMS
211
2.4.3.3
CARBON
AND
CARBON-COATED
PLASTIC
SUPPORT
FILMS
212
2.4.3
A
PREPARATION
OF
HYDROPHILIC
FILMS
BY
GLOW-DISCHARGE
212
2.4.4
SPECIMEN
PREPARATION
TECHNIQUES
214
2.4.5
SAMPLE
PREPARATION
BY
RAPID
FREEZING
218
2.4.5.1
PRETREATMENT
OF
SUBCELLULAR
COMPONENTS
219
2.4.5.2
PRETREATMENT
OF
CELLS
220
2.4.5.3
FREEZING
IN
LIQUID
NITROGEN
220
2.4.5.4
PLUNGE
FREEZING
220
2.4.5.5
FREEZING
ON
COLD
SURFACES
(COLD
BLOCK
FREEZING,
"
SLAMMING
"
)
223
2.4.5.6
HIGH-PRESSURE
FREEZING
224
2.4.5.7
FREEZE-FRACTURING
AND
FREEZE-ETCHING
224
2.4.6
SAMPLE
PREPARATION
FOR
THIN
SECTIONING
230
2.4.6.1
CHEMICAL
FIXATION
230
2.4.6.2
DEHYDRATION,
INFILTRATION,
AND
EMBEDDING
232
2.4.6.3
NANOPLAST
RESIN
EMBEDDING
235
2.4.6.4
EMBEDDING
MEDIA
FOR
IMMUNOCYTOCHEMISTRY
236
2.4.6.5
FREEZE-SUBSTITUTION
238
2.4.7
ULTRAMICROTOMY
239
2.4.7.1
TRIMMING
239
2.4.7.2
KNIVES
240
2.4.7.3
ULTRA-THIN
SECTIONING
242
2.4.7.4
POST-STAINING
OF
SECTIONS
243
2.4.7.5
CRYO-ULTRAMICROTOMY
245
2.4.8
LOCALIZATION
AND
STRUCTURE
OF
MACROMOLECULES
247
2.4.8.1
PREPARATION
OF
MARKER
SYSTEMS
FOR
LOCALIZATION
PROCEDURES
249
2.4.8.2
PREPARATION
OF
COLLOIDAL
GOLD
253
2.4.8.3
PREPARATION
OF
WHEAT-GERM
AGGLUTININ/BOVINE
SERUM
ALBUMIN
(BSA)
253
2.4.8.4
PRETREATMENT
OF
PRIMARY
ANTIBODIES
254
2.4.8.5
LOCALIZATION
PROCEDURES
254
2.4.8.6
IMMUNOGOLD
LABELING
OF
CRYOSECTIONS
255
2.4.8.7
CONTROL
EXPERIMENTS
IN
IMMUNOCYTOCHEMISTRY
257
2.4.8.8
LABELING
OF
EPITOPES
ON
ISOLATED
PROTEIN
MOLECULES
258
2.4.8.9
2-D
CRYSTALS
OF
MEMBRANE
PROTEINS
258
2.4.8.10
EMERGING
TECHNIQUES
AND
CONCEPTS
FOR
PROTEIN
CRYSTALLIZATON
265
XII
I
CONTENTS
2.4.9
IMAGING
AND
IMAGE
EVALUATION
UNDER
THE
TRANSMISSION
ELECTRON
MICROSCOPE
267
2.4.9.1
2.4.9.2
2.4.93
2.4.9.4
2.4.10
STEREO
IMAGES
268
FOURIER-TRANSFORMED
IMAGES
270
ELECTRON
DIFFRACTION
272
DETERMINATION
OF
MACROMOLECULAR
STRUCTURE
BY
PARTICLE
AVERAGING
272
STANDARD
SPECIMEN
PREPARATION
FOR
SCANNING
ELECTRON
MICROSCOPY
277
3
3.1
3.2
3.3
3.3.1
3.3.2
3.3.3
3.4
3.5
3.6
3.7
3.8
3.9
3.10
3.10.1
3.10.2
3.11
OTHER
MICROSCOPICAL
METHODS
AND
THEIR
APPLICATION
POTENTIAL
287
TOTAL
INTERNAL
REFLECTION
MICROSCOPY
287
MULTIPHOTON
EXCITATION
MICROSCOPY
290
ANALYTICAL
FEATURES
OF
FLUORESCENCE
MICROSCOPES
291
FLUORESCENCE
RECOVERY
AFTER
PHOTOBLEACHING
(FRAP)
292
FLUORESCENCE
LOESS
IN
PHOTOBLEACHING
(FLIP)
292
FLUORESCENCE
RESONANCE
ENERGY
TRANSFER
(FRET)
292
FLUORESCENCE
CORRELATION
SPECTROSCOPY
293
SOFT
X-RAY
MICROSCOPY
296
MICROTOMOGRAPHIC
TECHNIQUES
298
MICROSCOPIC
IMAGING
WITH
PROTONS
AND
HEAVY
IONS
302
ULTRASOUND
BIOMICROSCOPY
AND
SCANNING
ACOUSTIC
MICROSCOPY
304
OPTICAL
COHERENCE
MICROSCOPY/TOMOGRAPHY
307
SCANNING
PROBE
MICROSCOPY
308
THE
SCANNING
TUNNELING
MICROSCOPE
(STM)
309
ATOMIC
FORCE
MICROSCOPY
AND
RELATED
TECHNIQUES
310
NEAR-FIELD
SCANNING
OPTICAL
MICROSCOPY
315
INDEX
321 |
| any_adam_object | 1 |
| author | Hoppert, Michael |
| author_facet | Hoppert, Michael |
| author_role | aut |
| author_sort | Hoppert, Michael |
| author_variant | m h mh |
| building | Verbundindex |
| bvnumber | BV016449383 |
| callnumber-first | Q - Science |
| callnumber-label | QH207 |
| callnumber-raw | QH207 |
| callnumber-search | QH207 |
| callnumber-sort | QH 3207 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | UH 6700 WC 2900 WF 9700 |
| classification_tum | CIT 978f BIO 040f PHY 135f PHY 131f |
| ctrlnum | (OCoLC)46984701 (DE-599)BVBBV016449383 |
| dewey-full | 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6 |
| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Physik Biologie Chemie-Ingenieurwesen Biotechnologie |
| format | Book |
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| id | DE-604.BV016449383 |
| illustrated | Illustrated |
| indexdate | 2024-08-23T00:23:22Z |
| institution | BVB |
| isbn | 3527301984 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010171132 |
| oclc_num | 46984701 |
| open_access_boolean | |
| owner | DE-20 DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-1046 DE-19 DE-BY-UBM DE-526 DE-634 DE-11 DE-B768 |
| owner_facet | DE-20 DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-1046 DE-19 DE-BY-UBM DE-526 DE-634 DE-11 DE-B768 |
| physical | XII, 330 S. Ill., graph. Darst. : 25 cm |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Hoppert, Michael Verfasser aut Microscopic techniques in biotechnology Michael Hoppert Weinheim Wiley-VCH 2003 XII, 330 S. Ill., graph. Darst. : 25 cm txt rdacontent n rdamedia nc rdacarrier Literaturangaben Biotechnologie Microscopie Biotechnology Microscopy Microscopy methods Biotechnologie (DE-588)4069491-4 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Biotechnologie (DE-588)4069491-4 s Mikroskopie (DE-588)4039238-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010171132&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hoppert, Michael Microscopic techniques in biotechnology Biotechnologie Microscopie Biotechnology Microscopy Microscopy methods Biotechnologie (DE-588)4069491-4 gnd Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4069491-4 (DE-588)4039238-7 |
| title | Microscopic techniques in biotechnology |
| title_auth | Microscopic techniques in biotechnology |
| title_exact_search | Microscopic techniques in biotechnology |
| title_full | Microscopic techniques in biotechnology Michael Hoppert |
| title_fullStr | Microscopic techniques in biotechnology Michael Hoppert |
| title_full_unstemmed | Microscopic techniques in biotechnology Michael Hoppert |
| title_short | Microscopic techniques in biotechnology |
| title_sort | microscopic techniques in biotechnology |
| topic | Biotechnologie Microscopie Biotechnology Microscopy Microscopy methods Biotechnologie (DE-588)4069491-4 gnd Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Biotechnologie Microscopie Biotechnology Microscopy Microscopy methods Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010171132&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hoppertmichael microscopictechniquesinbiotechnology |