E. coli plasmid vectors: methods and applications
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2003
|
| Schriftenreihe: | Methods in molecular biology
235 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | xi, 316 p. ill. : 24 cm |
| ISBN: | 1588291510 |
Internformat
MARC
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| 245 | 1 | 0 | |a E. coli plasmid vectors |b methods and applications |c edited by Nicola Casali and Andrew Preston |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2003 | |
| 300 | |a xi, 316 p. |b ill. : 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 235 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Escherichia coli |2 gtt | |
| 650 | 7 | |a Plasmiden |2 gtt | |
| 650 | 7 | |a Vectoren (biochemie) |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Cloning, Molecular |x methods |v Laboratory Manuals | |
| 650 | 4 | |a DNA, Recombinant |v Laboratory Manuals | |
| 650 | 4 | |a Escherichia coli |v Laboratory manuals | |
| 650 | 4 | |a Escherichia coli |x genetics |v Laboratory Manuals | |
| 650 | 4 | |a Genetic vectors |v Laboratory manuals | |
| 650 | 4 | |a Plasmids |v Laboratory manuals | |
| 650 | 4 | |a Plasmids |x genetics |v Laboratory Manuals | |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Plasmid |0 (DE-588)4046270-5 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804129774184955904 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
1 The Function and Organization of Plasmids
Finbarr Hayes 1
2 Choosing a Cloning Vector
Andrew Preston 19
3 Escherichia coli Host Strains
Nicola Casali 27
4 Chemical Transformation of E. coli
W. Edward Swords 49
5 Electroporation of E. coli
Claire A. Woodall 55
6 DNA Transfer by Bacterial Conjugation
Claire A. Woodall 61
7 Cosmid Packaging and Infection of E. coli
MalloryJ. A. White and Wade A. Nichols 67
8 Isolation of Plasmids from E. coli by Alkaline Lysis
Sabine Ehrt and Dirk Schnappinger 75
9 Isolation of Plasmids from E. coli by Boiling Lysis
Sabine Ehrt and Dirk Schnappinger 79
10 High Purity Plasmid Isolation Using Silica Oxide
Stefan Grimm and Frank Voli Neudecker 83
11 High Throughput Plasmid Extraction Using Microtiter Plates
Michael A. Quail 89
12 Isolation of Cosmid and BAC DNA from E. coli
Daniel Sinnett and Alexandre Montpetit 99
13 Preparation of Single Stranded DNA from Phagemid Vectors
W. Edward Swords 103
14 Using Desktop Cloning Software to Plan, Track,
and Evaluate Cloning Projects
Robert H. Gross 107
15 Cloning in Plasmid Vectors
Carey Pashley and Sharon Kendall 121
vii
via Contents
16 Extraction of DNA from Agarose Gels
Nicholas Downey 137
17 Cloning PCR Products with T Vectors
Wade A. Nichols 141
18 Construction of Genomic Libraries in ^ Vectors
Yilun Wang, Zheng Cao, Darryl Hood,
and James G. Townsel 153
19 Rapid Screening of Recombinant Plasmids
SangweiLu 169
20 Restriction Analysis of Recombinant Plasmids
Joanne Goranson Siekierke and Jarrod L. Erbe 175
21 Screening Recombinant DNA Libraries
Wade A. Nichols 183
22 Sequencing Using Fluorescent Labeled Nucleotides
Allison F. Gillaspy 195
23 Site Directed Mutagenesis Using the Megaprimer Method
ZhidongXu, Alessia Colosimo, and Dieter C. Gruenert 203
24 Site Directed Mutagenesis by Inverse PCR
Clifford N. Dominy and David W. Andrews 209
25 Creating Nested DNA Deletions Using Exonuclease III
Rosamund Powles and Lafras M. Steyn 225
26 Transposon and Transposome Mutagenesis
of Plasmids, Cosmids, and BACs
Alistair McGregor 233
27 In Vitro Transcription and Translation
Farahnaz Movahedzadeh, Susana Gonzalez Rico,
and Robert A. Cox 247
28 Vectors for the Expression of Recombinant Proteins in E. coli
Sally A. Cantrell 257
29 Expression of Recombinant Proteins from lac Promoters
Charles R. Sweet 277
30 Plasmid Based Reporter Genes: Assays for p Galactosidase and
Alkaline Phosphatase Activities
Minghsun Liu 289
31 Plasmid Based Reporter Genes: Assays for Green
Fluorescent Protein
Sergei R. Doulatov 297
Index 305
|
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| spelling | E. coli plasmid vectors methods and applications edited by Nicola Casali and Andrew Preston Totowa, N.J. Humana Press 2003 xi, 316 p. ill. : 24 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 235 Includes bibliographical references and index Escherichia coli gtt Plasmiden gtt Vectoren (biochemie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cloning, Molecular methods Laboratory Manuals DNA, Recombinant Laboratory Manuals Escherichia coli Laboratory manuals Escherichia coli genetics Laboratory Manuals Genetic vectors Laboratory manuals Plasmids Laboratory manuals Plasmids genetics Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd rswk-swf Plasmid (DE-588)4046270-5 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Escherichia coli (DE-588)4070959-0 s Plasmid (DE-588)4046270-5 s Labortechnik (DE-588)4123602-6 s DE-604 Casali, Nicola Sonstige oth Preston, Andrew Sonstige oth Methods in molecular biology 235 (DE-604)BV035362695 235 http://www.loc.gov/catdir/toc/fy038/2002191946.html Table of contents HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010164398&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | E. coli plasmid vectors methods and applications Methods in molecular biology Escherichia coli gtt Plasmiden gtt Vectoren (biochemie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cloning, Molecular methods Laboratory Manuals DNA, Recombinant Laboratory Manuals Escherichia coli Laboratory manuals Escherichia coli genetics Laboratory Manuals Genetic vectors Laboratory manuals Plasmids Laboratory manuals Plasmids genetics Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd Plasmid (DE-588)4046270-5 gnd Escherichia coli (DE-588)4070959-0 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4046270-5 (DE-588)4070959-0 (DE-588)4143413-4 |
| title | E. coli plasmid vectors methods and applications |
| title_auth | E. coli plasmid vectors methods and applications |
| title_exact_search | E. coli plasmid vectors methods and applications |
| title_full | E. coli plasmid vectors methods and applications edited by Nicola Casali and Andrew Preston |
| title_fullStr | E. coli plasmid vectors methods and applications edited by Nicola Casali and Andrew Preston |
| title_full_unstemmed | E. coli plasmid vectors methods and applications edited by Nicola Casali and Andrew Preston |
| title_short | E. coli plasmid vectors |
| title_sort | e coli plasmid vectors methods and applications |
| title_sub | methods and applications |
| topic | Escherichia coli gtt Plasmiden gtt Vectoren (biochemie) gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cloning, Molecular methods Laboratory Manuals DNA, Recombinant Laboratory Manuals Escherichia coli Laboratory manuals Escherichia coli genetics Laboratory Manuals Genetic vectors Laboratory manuals Plasmids Laboratory manuals Plasmids genetics Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd Plasmid (DE-588)4046270-5 gnd Escherichia coli (DE-588)4070959-0 gnd |
| topic_facet | Escherichia coli Plasmiden Vectoren (biochemie) Wetenschappelijke technieken Wissenschaftliches Arbeiten Cloning, Molecular methods Laboratory Manuals DNA, Recombinant Laboratory Manuals Escherichia coli Laboratory manuals Escherichia coli genetics Laboratory Manuals Genetic vectors Laboratory manuals Plasmids Laboratory manuals Plasmids genetics Laboratory Manuals Labortechnik Plasmid Aufsatzsammlung |
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