Liposomes: a practical approach
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Oxford Univ. Press
2003
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | The practical approach series
264 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Hier auch später erschienene, unveränderte Nachdrucke |
| Beschreibung: | XXIII, 396 S. Ill., graph. Darst. |
| ISBN: | 0199636559 0199636540 9780199636549 |
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| 245 | 1 | 0 | |a Liposomes |b a practical approach |c ed. by Vladimir Torchilin ... |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Oxford [u.a.] |b Oxford Univ. Press |c 2003 | |
| 300 | |a XXIII, 396 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a The practical approach series |v 264 | |
| 500 | |a Hier auch später erschienene, unveränderte Nachdrucke | ||
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| 650 | 4 | |a Liposomes | |
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Datensatz im Suchindex
| _version_ | 1804129773997260800 |
|---|---|
| adam_text | Contents
list of protocols page xXii
Abbreviations xix
I General methods
1 Preparation of llposomes 3
J. Lasch, V. Weissig, and M. Brandl
1 Handling and storage of lipids 3
2 Preparation methods 4
Hand shaken vesicles 4
Sonicated vesicles 7
Freeze dried rehydration vesicles 9
Reverse phase evaporation 10
Detergent depletion 12
High pressure homogenization 16
3 Purification of liposomes 23
Column filtration 24
Purification of liposomes by centrifugation 25
References 27
2 Characterization of liposomes 31
Nkolaas]an Zuidam, Setnco de Vrueh, and DaanJ. A Crommelin
1 Introduction 31
2 Chemical analysis of liposomal components 33
Spectrophotometric quantification of phospholipids 33
Thin layer chromatography of lipids 35
HPLC analysis of (phospho)lipids 39
HPLC analysis of cholesterol and a tocopherol 41
GLC analysis of fatty acids 42
Chemical analysis of degradation of liposomal phospholipids 45
3 Physical characterization of liposomes 50
Determination of percentage capture 50
Determination of percentage release 54
Determination of entrapped volume 64
Lamellarity 64
Size determination of liposomes 67
Zeta potential measurements based on PCS 72
vii
4 Miscellaneous methods 72
Bligh and Dyer extraction 72
Sep Pak minlcolumn extraction 74
Preparation of carboxyfluorescein and calcein solutions 75
References 77
3 Physical methods of study: differential scanning calorimetry 79
Kevin M. G. Taylor and Duncan Q. M, Craig
1 Introduction 79
2 Overview of techniques 79
Differential scanning calorimetry 79
High sensitivity differential scanning calorimetry 82
Modulated temperature differential scanning calorimetry 82
3 Practical aspects of making DSC measurements M
Choice of pans 84
Sample size 85
Calibration 85
Scanning rate 86
Conditions required for liposomal studies 87
Practicalities of HSDSC measurements 89
Practicalities of MTDSC measurements 90
4 Application of DSC and related techniques to the study of liposomes 92
Phase transition behaviour of liposomes 92
Phase transition of multi component systems 95
Phospholipid drug interactions 96
DSC studies of liposomes as model membranes 99
MTDSC of freeze dried liposomes 99
Acknowledgements 103
References 101
4 Fluorescence methods in liposome research 105
Nejat Dihgunes, Luis A BagatoUi, Paul Meers, Yu Kyumg Oh, and Robert M. Straubinger
1 Fluorescence assays for liposome fusion 105
The terbium/dipicolinic acid assay for intermixing of aqueous contents 105
The aminonaphthalene trisulfonic acid/p xylylene bis(pyridinium) bromide
(ANTS/DPX) assay for aqueous contents mixing 112
The NBD/rhodamine resonance energy transfer assay for lipid mixing 113
A fluorescence assay for intermixing of inner monolayers 117
2 Fluorescence assays for liposome permeability 118
Release of carboxyfluorescein or calcein 118
Release oftheTb/DPA complex 118
The release of ANTSpPX 119
3 Fluorescent detection of protein binding to phospholipid membranes 119
Detection via membrane associated fluorophores 120
Detection with intrinsic protein associated fluorophores 123
4 liposomes as pH sensors in the study of cellular interactions 126
The pyranine (HPTS) assay 126
The dual fluorophore assay 132
viii
5 Two photon excitation fluorescence microscopy to detect lipid phases in giant
liposomes 135
Direct visualization of lipid domain coexistence in bilayers 135
Giant unilamellar liposomes 136
Two photon excitation microscopy 138
Visualization of gel/fluid phase coexistence in binary phospholipid mixtures 143
Acknowledgements 143
: References 143
5 Stability, storage, and sterilization of liposomes 149
; Mcolaas Jan Zuidam, Ewoud van Winden, Remco de Vrueh, and Daan]. A. Crommelin
1 Introduction 149
2 Prevention of chemical degradation 149
Phospholipid oxidation 149
Hydrolysis of phospholipids 152
3 Prevention of physical changes 154
4 Freeze drying of liposomes 155
Introduction 155
Important factors for freeze drying of liposomes 156
Stability in the freeze dried state 161
Example of a freeze drying protocol 162
5 Preparation of sterile liposome formulations 364
References 164
6 Encapsulation of weakly basic drugs, antisense oligonucleotides, and
plasmid DNA within large unilamellar vesicles for drug delivery
applications 167
David B. Fenske, Norbert Maurer, and Pteter R. CuHis
1 Introduction 167
2 Measurement of phospholipid concentration 169
3 The formation of large unilamellar vesicles by extrusion through
polycarbonate filters with denned pore size 171
4 Accumulation of weakly basic drugs within LUVs in response to
transmembrane pH gradients 173
5 Iiposomal systems for the encapsulation of genetic drugs: long circulating
vectors for the systemic delivery of genes and antisense
oligonucleotides 181
; References 188
7 Surface modification of liposomes 193
i Vladimir P. TorchUin, VoVcmar Weissig, Francis]. Martin, Timothy D. Heath, and
I RogerHC.New
1 Introduction 193
2 Protein and peptide attachment to the Iiposomal surface 194
Binding of proteins and peptides to liposomes via amino groups 194
, Binding of proteins to liposomes via sulfhydryl groups 204
: ix
3 Binding of carbohydrates and other small molecules to the liposomal
surface 216
4 Attachment of diagnostically significant reporter metal atoms to the liposome
surface 218
Conjugation of low molecular weight chelate DTPA with lipid for incorporation into
the liposomal membrane 219
Loading liposome associated chelates with metals 223
References 227
8 Long circulating sterically protected liposomes 231
Alexander L Klibanov, Vladimir P. TorcMin, and Samuel Zalipsky
1 Introduction 233
2 Attachment of sterically protecting polymer (PEG) to liposomes 232
Incorporation of PEG into liposomes 232
Attachment of PEG to previously prepared liposomes 235
Dependence ofbiodistribution on the size of long circulating liposomes 236
Tumour accumulation of long circulating liposomes 237
3 Coupling of various ligands to the distal end of liposome grafted polymer 238
Iigand attachment to hydrazide activated PEG 243
Iigand attachment to PDP activated PEG 246
Ligand attachment via COOH activated PEG 249
Coupling of primary amino group containing ligands to pNP PEG PE containing
liposomes 250
ligand attachment by preparation of ligand PEG ligand conjugates and their
incorporation into liposomes 253
4 Detachable PEG on the liposome surface 261
5 Biological factors responsible for the clearance of PEGylated liposomes 262
References 263
9 Liposomes in biological systems 267
Jan A A M. Kamps and Gerrit L Scherphof
1 Introduction 267
2 Interaction of liposomes with cells 267
Possible liposome cell interaction: adsorption, (receptor mediated) endocytosis,
lipid exchange, fusion 267
Markers 269
Cell lines and primary cell cultures 270
Isolation and culturing of liver cells 273
3 Biodistribution of liposomes 279
Animal models; species differences 279
In vivo liposome cell interactions 280
Markers 281
Liposome kinetics and tissue distribution 282
Acknowledgements 286
References 286
10 Cationic liposomes In gene delivery 289
Sean Sullivan, Yan Gong, and Jeffrey Busies
1 Introduction 289
x
2 Plasmid DNA isolation 296
3 Quantification of plasmid DNA concentration 298
4 Calculation of N/P ratio 300
References 301
II Selected topics and applications
H pH sensitive liposomes 305
ReginePeschka Siiss and Rolf Schubert
1 Introduction 305
2 Mechanisms of pH sensitivity 307
j 3 Methods 311
: Preparation of pH sensitive liposomes 311
Characterization of pH sensitive liposomes 314
Cellular association of pH sensitive liposomes 314
References 316
12 Radiolabelled liposomes for imaging and blodistributlon studies 319
Beth A. Gains and William T. Phillips
1 Introduction 319
2 Instrumentation for tracking radiolabelled liposomes 320
Gamma camera 320
Positron emission Monographic camera 322
Gamma well counter 322
3 Methods for labelling liposomes with gamma emitting radionuclides 323
i General overview 323
; Radionuclides available for liposome labelling methods 324
Methods for labelling liposomes using radionuclides 325
4 Characterization of radiolabelled liposomes 329
i In vitro radiolabel stability evaluation 329
i In vivo radiolabel evaluation 329
5 Image acquisition and analysis methods 330
6 Tissue biodistribution method using gamma well counter 334
References 335
13 Isothermlc titration calorimetry (ITC) 337
J. lasch
1 Introduction 337
2 Instrument and the principle of technique 337
3 Computer assisted measurement with the MCS ITC 340
Making a calorimetric titration 340
4 Estimation of critical micellar concentration 342
Thermodynamic background 342
Measurement 344
5 Surfactant bilayer membrane equilibria 347
Surfactant partitioning into lipid membranes 347
Solubilization of lipsomes 349
References 351
xi
14 Vesicular phospholipld gels 353
M. Brondl and U. Massing
1 Background 353
Biopharmaceutical and technological challenges with liposomes for i.v. application 353
Biopharmaceutical and technological challenges with liposomes for loco regional
administration 354
Phospholipid dispersions: swelling and phases 354
Mechanical stress: formulation of vesicle dispersions and vesicular phospholipid gels 354
The vesicular phospholipid gel concept 355
2 Preparation of vesicular phospholipid gels 355
VPGs made of a single phospholipid 355
VPGs made of two or more lipids 357
3 Loading of VPGs with drugs 358
Direct entrapment 359
Passive loading 359
Incorporation/association 360
4 Sterilization of VPGs 361
5 Characteristics of VPGs 361
Morphology 361
Rheology, viscosity 362
Sustained release behaviour in vitro 362
6 Preparation of SUV dispersions from VPGs 365
7 Characterization of SUV dispersions prepared from VPGs 365
Vesicle size distribution 365
Encapsulation efficiency 366
8 In vivo applications 367
Preparation and in vitro characterization of Gemcitabine containing VPG 368
Intra tumoral application of Gemcitabine containing VPG 369
i.v. application of Gemcitabine containing VPG 369
References 371
15 Liposome based DNA vaccines: procedures for entrapment 373
Gregory Gregoriadis, Brenda McCormack, Yvonne feme, Andrew Bacon, Wilson Caparros Wanderley,
and Brahim Zadi
1 Introduction 373
2 Materials 374
3 Entrapment of plasmid DNA into liposomes by the dehydration rehydration
procedure 374
Solutions 374
Procedure steps 375
References 380
Appendix 3si
Index 389
xii
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| indexdate | 2024-07-09T19:10:28Z |
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| language | English |
| lccn | 2002045045 |
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| physical | XXIII, 396 S. Ill., graph. Darst. |
| publishDate | 2003 |
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| spelling | Liposomes a practical approach ed. by Vladimir Torchilin ... 2. ed. Oxford [u.a.] Oxford Univ. Press 2003 XXIII, 396 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 264 Hier auch später erschienene, unveränderte Nachdrucke Lipossomos (farmacologia) larpcal Liposomes Liposomes Handbooks, manuals, etc Liposom (DE-588)4122262-3 gnd rswk-swf Liposom (DE-588)4122262-3 s DE-604 Torčilin, Vladimir P. 1946- Sonstige (DE-588)112659381 oth The practical approach series 264 (DE-604)BV007921321 264 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010164289&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Liposomes a practical approach The practical approach series Lipossomos (farmacologia) larpcal Liposomes Liposomes Handbooks, manuals, etc Liposom (DE-588)4122262-3 gnd |
| subject_GND | (DE-588)4122262-3 |
| title | Liposomes a practical approach |
| title_auth | Liposomes a practical approach |
| title_exact_search | Liposomes a practical approach |
| title_full | Liposomes a practical approach ed. by Vladimir Torchilin ... |
| title_fullStr | Liposomes a practical approach ed. by Vladimir Torchilin ... |
| title_full_unstemmed | Liposomes a practical approach ed. by Vladimir Torchilin ... |
| title_short | Liposomes |
| title_sort | liposomes a practical approach |
| title_sub | a practical approach |
| topic | Lipossomos (farmacologia) larpcal Liposomes Liposomes Handbooks, manuals, etc Liposom (DE-588)4122262-3 gnd |
| topic_facet | Lipossomos (farmacologia) Liposomes Liposomes Handbooks, manuals, etc Liposom |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010164289&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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