Ancient DNA typing: methods, strategies and applications ; with 34 tables
"Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering."...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
2003
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | "Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering."--BOOK JACKET. |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XII, 298 S. Ill., graph. Darst. |
| ISBN: | 3540430377 |
Internformat
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| 100 | 1 | |a Hummel, Susanne |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Ancient DNA typing |b methods, strategies and applications ; with 34 tables |c Susanne Hummel |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2003 | |
| 300 | |a XII, 298 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturangaben | ||
| 520 | 1 | |a "Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering."--BOOK JACKET. | |
| 650 | 7 | |a Fossielen |2 gtt | |
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| 650 | 4 | |a Nucleotide sequence | |
| 650 | 4 | |a Polymerase Chain Reaction | |
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Datensatz im Suchindex
| _version_ | 1805083660633767936 |
|---|---|
| adam_text |
Contents
1 Introduction 1
References 10
2 DNA markers 19
2.1 Mitochondrial DNA 20
2.1.1 The hypervariable regions and control region V 23
2.1.2 Cytochrome b 25
2.2 Chromosomal DNA 26
2.2.1 Amelogenin 29
2.2.2 Autosomal STRs 30
2.2.3 Y chromosomal STRs 39
2.2.4 X chromosomal STRs 42
2.2.5 ABO blood group genes 43
2.2.6 CCR5 46
2.2.7 AF508 47
References 48
3 aDNA extraction 57
3.1 Comparison of extraction methods 58
3.2 aDNA yield 63
3.3 aDNA preservation 66
3.3.1 aDNA degradation patterns 66
3.3.2 The age of aDNA 68
3.3.3 Fragment lengths of aDNA 73
3.3.4 Storage of aDNA extracts 76
References 78
4PCR 81
4.1 Basic PCR mechanism 82
4.2 Number of amplification cycles 85
4.3 Cycling parameters 86
4.3.1 Annealing temperature 86
4.3.2 Denaturation temperature 87
x 1
4.3.3 Elongation temperature 88
4.3.4 Durations of the cycling steps 89
4.4 Primer design 90
4.4.1 Matching primers 91
4.4.2 Primer energy profile 92
4.4.3 Annealing temperature, primer dimer and hairpins 93
4.4.4 Mismatch primers 95
4.4.5 Software packages 96
4.4.6 Primer design step by step 96
4.5 Multiplex PCR 101
4.6 PCR failures 102
4.6.1 DNA degradation 102
4.6.2 Inhibition 104
4.6.3 DNA overload and overcycling 105
References 107
5 Analysis of PCR products Ill
5.1 Fragment length analysis 112
5.1.1 Agarose gel electrophoresis 113
5.1.2 Automated fragment length determination 115
5.2 Sequence analysis 120
5.2.1 Direct sequencing vs. sequencing clones 122
5.2.2 Overlapping sequences vs. a multiplex approach 124
5.3 RFLP analysis 126
References 128
6 Authenticity of results 131
6.1 Contamination sources 132
6.1.1 Contamination through handling 133
6.1.2 Contamination in reaction tubes 136
6.1.3 Cleaning the PCR reaction tubes 144
6.1.4 PCR in glass tubes 147
6.2 Validating aDNA results 150
6.3 Ring exercises 155
References 155
7 Applications 159
7.1 Sex determination 160
7.1.1 The children of Aegerten 160
7.1.2 Sex determination by multiplex amplification 164
7.2 Identification 165
7.2.1 A disturbed burial site 165
XI
7.2.2 Duke Christian II 168
7.2.3 Pieces of parchment 170
7.2.4 Sheep, goats and cattle 172
7.2.5 Species, sex and individual identification 173
7.2.6 Dog or fox 174
7.2.7 Elephants and rhinoceroses 176
7.2.8 Beef in the sausage 179
7.2.9 Rock wallabies on Depuch Island 181
7.3 Kinship 183
7.3.1 A Merovingian family 183
7.3.2 The earls of Konigsfeld 185
7.3.3 The Lichtenstein cave a place of sacrifice or a burial site? 188
7.3.4 Widukind 193
7.3.5 Orangutans 197
7.4 Population genetics 200
7.4.1 Marriage patterns in Weingarten 200
7.4.2 Expert knowledge inGoslar 204
7.4.3 ABO Blood groups 207
7.4.4 A32ccr5 already in the Bronze Age 211
7.4.5 AF 508 in Alia 214
7.4.6 Y chromosomal haplotypes and the region of origin 216
References 217
8 Protocols 225
8.1 Collection and storage of samples 225
8.2 Sample preparation 226
8.3 Phenol based DNA extraction 227
8.4 DNA extraction from saliva 230
8.5 PCR primers and protocols 231
8.6 Agarose gel electrophoresis 250
8.7 Taq cycle sequencing 253
8.8 PAA gel electrophoresis 254
8.9 RFLP digestion 258
8.10 Basic chemistry and reagents 259
References 268
Appendix 271
I Laboratory equipment 271
1.1 Pre PCR laboratory equipment 272
1.2 PCR laboratory 272
1.3 Post PCR laboratory 273
1.4 Photographic documentation 273
XII I
IIPCR trouble shooting 274
III Internet sites of interest 278
Subject index 287
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| spelling | Hummel, Susanne Verfasser aut Ancient DNA typing methods, strategies and applications ; with 34 tables Susanne Hummel Berlin [u.a.] Springer 2003 XII, 298 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben "Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering."--BOOK JACKET. Fossielen gtt Genanalyse gtt Prehistorie gtt Base Sequence DNA analysis DNA, Fossil Analysis Fossils Mitochondrial DNA Analysis Nucleotide sequence Polymerase Chain Reaction Polymerase chain reaction Paläopathologie (DE-588)4173117-7 gnd rswk-swf Mitochondriale DNS (DE-588)4202301-4 gnd rswk-swf DNA-typing (DE-588)4473129-2 gnd rswk-swf Fossiler Mensch (DE-588)4413624-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Nucleotidsequenz (DE-588)4297525-6 gnd rswk-swf Fossiler Mensch (DE-588)4413624-9 s Mitochondriale DNS (DE-588)4202301-4 s DNA-typing (DE-588)4473129-2 s Nucleotidsequenz (DE-588)4297525-6 s Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Paläopathologie (DE-588)4173117-7 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010152069&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hummel, Susanne Ancient DNA typing methods, strategies and applications ; with 34 tables Fossielen gtt Genanalyse gtt Prehistorie gtt Base Sequence DNA analysis DNA, Fossil Analysis Fossils Mitochondrial DNA Analysis Nucleotide sequence Polymerase Chain Reaction Polymerase chain reaction Paläopathologie (DE-588)4173117-7 gnd Mitochondriale DNS (DE-588)4202301-4 gnd DNA-typing (DE-588)4473129-2 gnd Fossiler Mensch (DE-588)4413624-9 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Nucleotidsequenz (DE-588)4297525-6 gnd |
| subject_GND | (DE-588)4173117-7 (DE-588)4202301-4 (DE-588)4473129-2 (DE-588)4413624-9 (DE-588)4256726-9 (DE-588)4297525-6 |
| title | Ancient DNA typing methods, strategies and applications ; with 34 tables |
| title_auth | Ancient DNA typing methods, strategies and applications ; with 34 tables |
| title_exact_search | Ancient DNA typing methods, strategies and applications ; with 34 tables |
| title_full | Ancient DNA typing methods, strategies and applications ; with 34 tables Susanne Hummel |
| title_fullStr | Ancient DNA typing methods, strategies and applications ; with 34 tables Susanne Hummel |
| title_full_unstemmed | Ancient DNA typing methods, strategies and applications ; with 34 tables Susanne Hummel |
| title_short | Ancient DNA typing |
| title_sort | ancient dna typing methods strategies and applications with 34 tables |
| title_sub | methods, strategies and applications ; with 34 tables |
| topic | Fossielen gtt Genanalyse gtt Prehistorie gtt Base Sequence DNA analysis DNA, Fossil Analysis Fossils Mitochondrial DNA Analysis Nucleotide sequence Polymerase Chain Reaction Polymerase chain reaction Paläopathologie (DE-588)4173117-7 gnd Mitochondriale DNS (DE-588)4202301-4 gnd DNA-typing (DE-588)4473129-2 gnd Fossiler Mensch (DE-588)4413624-9 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Nucleotidsequenz (DE-588)4297525-6 gnd |
| topic_facet | Fossielen Genanalyse Prehistorie Base Sequence DNA analysis DNA, Fossil Analysis Fossils Mitochondrial DNA Analysis Nucleotide sequence Polymerase Chain Reaction Polymerase chain reaction Paläopathologie Mitochondriale DNS DNA-typing Fossiler Mensch Polymerase-Kettenreaktion Nucleotidsequenz |
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