Utilisation of evanescent fields for the characterisation of thin biosensing layer systems:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2002 |
| Beschreibung: | 137 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Utilisation of evanescent fields for the characterisation of thin biosensing layer systems |c Thomas Zacher |
| 264 | 1 | |c 2002 | |
| 300 | |a 137 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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|---|---|
| adam_text |
TABLE OF CONTENTS
1 SUMMARY.5
2 INTRODUCTION.7
3 FUNDAMENTALS.11
3.1 SURFACE PLASMON RESONANCE
.R~R.11
3.1.1 SURFACE PLASMON WAVES
.11
3.1.2 THE KRETSCHMANN-CONFIGURATION
.14
3.1.3 PENETRATION DEPTH OF SP WAVES
.17
3.1.4 REFLECTIVITY OF A LAYER SYSTEM
.18
3.2 BIOMOLECULAR INTERACTION ANALYSIS WITH SPR.21
3.2.1 THE LANGMUIR-ISOTHERM
.22
3.2.2 SPR BIOSENSOR DATA EVALUATION
.24
3.3
BIOSENSING HYDROGELS FOR SPR DETECTION.30
3.3.1 DEXTRAN HYDROGELS
.32
3.3.2 SYNTHETIC HYDROGELS
.37
4 AIM OF THE RESEARCH.41
5 THE SPR SETUP.?.43
5.1
GENERAL DESCRIPTION.43
5.2 ANGLE CALIBRATION.47
5.3
THE AVAILABLE MEASUREMENT MODES.49
5.3.1 ANGLE MODE.49
5.3.2 DIFFERENTIAL INTENSITY MODE
.;.50
6
THERMO-RESPONSIVE HYDROGELS.53
6.
1 SYNTHESIS OF POLYMERS.53
6.2 LCST OF AQUEOUS SOLUTIONS.58
6.3
LCST IN SURFACE-ATTACHED STATE.59
6.3.1 CONCEPT OF THE INVESTIGATION.59
6.3.2 EXPERIMENTAL PART
.62
6.3.2.1 PREPARATION OF SAMPLES.62
6.3.2.2 TEMPERATURE VARIANCE OF SOLVENT.63
6.3.2.2.1 CONTINUOUS HEATING.63
6.3.2.2.2 INJECTION.65
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/964927977
2
TABLE OF CONTENTS
6.3.3 RESULTS
.
66
6.3.4 DISCUSSION
. 59
6.3.5 CONCLUSION
.
74
6.4 PHOTOCROSSLINKING OF CINNAMOYLATED P-HPMA.75
6.4.1 EXPERIMENTAL PROCEDURE
.75
6.4.2 RESULTS AND DISCUSSION
.77
6.4.3 CONCLUSION
.
80
6.5 STUDY OF NON-SPECIFIC ADSORPTION.80
6.5.1 NON-SPECIFIC ADSORPTION - A COMPLEX PROBLEM
.
80
6.5.2 BOVINE SERUM ALBUMIN
.
82
6.5.3 SURFACE MODIFICATION AND BSA EXPOSURE
.
83
6.5.3.1 CHEMICALS.83
6.5.3.2 SYNTHESIS OF AMINODEXTRAN.84
6.5.3.3 PREPARATION OF SAMPLES.84
6.5.3.3.1 GOLD SURFACE.84
6.5.3.3.2 HEXADECYLMERCAPTAN SAM.84
6.53.3.3 UNDECANOIC ACID SAM + AMINODEXTRAN.84
6.5.33.4 P-THMA AND THERMO-RESPONSIVE HYDROGELS.84
6.53.4 PREPARATION OF HEPES-BUFFERED SALINE.85
6.53.5 EXPERIMENTAL PROCEDURE.85
6.5.4 RESULTS AND DISCUSSION
.
85
6.5.4.1 150GG/ML BSA IN HBS.85
6.5.4.2 2MG/ML BSA IN H
20.87
6.5.5 CONCLUSION
.
89
6.6 BIO-FUNCTIONALISATION OF ACETYLATED P-THMA.90
6.6.1 IMMUNOGLOBULIN G
.
90
6.6.2 PROTEIN A
.9/'
6.6.3 CHEMICALS
.
91
6.63.1 PREPARATION OF BUFFERS.91
6.6.4 IMMOBILISATION OF PROTEIN A
.
91
6.6.4.1 ACTIVATION CHEMISTRY AND PROTOCOL.92
6.6.4.2 IMMOBILISATION KINETICS.93
6.6.5 BIOMOLECULAR INTERACTION PRO TEIN A
-
IGG
.
95
6.6.5.1 EXPERIMENTAL PROCEDURE.95
6.6.5.2 INTERACTION AND REGENERATION KINETICS.95
6.6.6 CONCLUSION
.97
7 BINDING PROCESSES IN DEXTRAN HYDROGELS.99
7.1 CONCEPT OF THE INVESTIGATION.99
7.
1.1 THE EMPLOYED WAVELENGTHS
.
100
7.1.2 DIFFERENT BINDING REGIMES - DIFFERENT TIME-DEPENDENCE
.
102
7.1.3 THE THREE-ZONE MODEL
.
102
7.2 SURFACE MODIFICATION AND BIORECOGNITION PROCEDURES.105
7.2.1 THE STREPTAVIDIN - BIOTIN COMPLEX
.
105
7.2.2 CHEMICALS
.
106
7.2.2.1 PREPARATION OF BUFFERS.106
3
12.2.2
PREPARATION OF LIPOSOME SOLUTION.107
7.2.3 SURFACE CHEMISTRY.107
7.2.4 INTERACTION EXPERIMENTS
.107
7.3 RESULTS
AND
DISCUSSION.109
7.3.1 DETERMINATION OF THE OPTICAL PROPERTIES OF THE INITIAL HYDROGEL
LAYER SYSTEM AND
EMPLOYED SOLUTIONS
.109
7.3.2 EQUILIBRIUM SPR SIGNALS DURING BINDING EXPERIMENTS AND COMPARATIVE
SIMULATION. 113
7.3.3 TIME-DEPENDENCE OF BINDING PROCESSES
.119
7.3.3.1 IMMOBILISATION OF STREPTAVIDIN.119
7.3.3.2 BINDING OF BIOTINYLATED PROTEIN A TO STREPTAVIDIN.122
7.4
CONCLUSION
.125
REFERENCES.127
ACKNOWLEDGEMENT.139 |
| any_adam_object | 1 |
| author | Zacher, Thomas 1968- |
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| author_facet | Zacher, Thomas 1968- |
| author_role | aut |
| author_sort | Zacher, Thomas 1968- |
| author_variant | t z tz |
| building | Verbundindex |
| bvnumber | BV014589280 |
| classification_tum | PHY 666d PHY 855d |
| ctrlnum | (OCoLC)216883164 (DE-599)BVBBV014589280 |
| discipline | Physik Biologie |
| format | Book |
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| indexdate | 2024-10-09T18:04:07Z |
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| language | English |
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| physical | 137 S. Ill., graph. Darst. |
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| spelling | Zacher, Thomas 1968- Verfasser (DE-588)123925169 aut Utilisation of evanescent fields for the characterisation of thin biosensing layer systems Thomas Zacher 2002 137 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2002 Evaneszente Welle (DE-588)4338639-8 gnd rswk-swf Dünne Schicht (DE-588)4136925-7 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf Oberflächenplasmon (DE-588)4172251-6 gnd rswk-swf Hydrogel (DE-588)4160909-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hydrogel (DE-588)4160909-8 s Dünne Schicht (DE-588)4136925-7 s Biosensor (DE-588)4193016-2 s Oberflächenplasmon (DE-588)4172251-6 s Evaneszente Welle (DE-588)4338639-8 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009918548&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Zacher, Thomas 1968- Utilisation of evanescent fields for the characterisation of thin biosensing layer systems Evaneszente Welle (DE-588)4338639-8 gnd Dünne Schicht (DE-588)4136925-7 gnd Biosensor (DE-588)4193016-2 gnd Oberflächenplasmon (DE-588)4172251-6 gnd Hydrogel (DE-588)4160909-8 gnd |
| subject_GND | (DE-588)4338639-8 (DE-588)4136925-7 (DE-588)4193016-2 (DE-588)4172251-6 (DE-588)4160909-8 (DE-588)4113937-9 |
| title | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems |
| title_auth | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems |
| title_exact_search | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems |
| title_full | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems Thomas Zacher |
| title_fullStr | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems Thomas Zacher |
| title_full_unstemmed | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems Thomas Zacher |
| title_short | Utilisation of evanescent fields for the characterisation of thin biosensing layer systems |
| title_sort | utilisation of evanescent fields for the characterisation of thin biosensing layer systems |
| topic | Evaneszente Welle (DE-588)4338639-8 gnd Dünne Schicht (DE-588)4136925-7 gnd Biosensor (DE-588)4193016-2 gnd Oberflächenplasmon (DE-588)4172251-6 gnd Hydrogel (DE-588)4160909-8 gnd |
| topic_facet | Evaneszente Welle Dünne Schicht Biosensor Oberflächenplasmon Hydrogel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009918548&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zacherthomas utilisationofevanescentfieldsforthecharacterisationofthinbiosensinglayersystems |