Methods in cell-matrix adhesion:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Academic Press
2002
|
| Schriftenreihe: | Methods in cell biology
69 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 461 S. Ill., graph. Darst. |
| ISBN: | 0125441681 012044142X |
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| adam_text | B E - D - H* S~ METHODS IN CELL BIOLOGY PREPARED UNDER THE AUSPICES OF
THE AMERICAN SOCIETY FOR CELL BIOLOGY VOLUME 69 METHODS IN CELL-MATRIX
ADHESION EDITED BY JOSEPHINE C. ADAMS DEPARTMENT OF CELL BIOLOGY LERNER
RESEARCH INSTITUTE CLEVELAND CLINIC FOUNDATION CLEVELAND, OHIO ^
ACADEMIC PRESS ^ AN IMPRINT OF ELSEVIER SCIENCE AMSTERDAM BOSTON
LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN FRANCISCO SINGAPORE SYDNEY
TOKYO CONTENTS CONTRIBUTORS XI ACKNOWLEDGMENTS XV PART I PREFACE AND
PERSPECTIVES ON CELL-MATRIX ADHESION PREFACE JOSEPHINE C. ADAMS 3
CELL-MATRIX INTERACTIONS: THE VIEW FROM THE OUTSIDE PAUL BORNSTEIN 7
MATRIX AND MEANING MARTIN A. SCHWARTZ 13 CELL*MATRIX ADHESION RESEARCH
AND THE DEVELOPMENT OF BIOTHERAPEUTICS ROY R. LOBB 17 PART II MATRIX
METHODOLOGIES 1. DETECTION AND PURIFICATION OF INSTRUCTIVE EXTRACELLULAR
MATRIX COMPONENTS WITH MONOCLONAL ANTIBODY TECHNOLOGIES SUSANA G. GIL,
RANDY O. SIGH, AND WILLIAM G. CARTER I. INTRODUCTION 28 II. METHODS FOR
DETECTING ECM COMPONENTS THAT INSTRUCT CELL RESPONSES IN REPORTER CELLS
32 III. PREPARATION OF MABS FOR IDENTIFICATION OF INSTRUCTIVE ECM 41 IV.
PURIFICATION OF INSTRUCTIVE ECM WITH MABS AND INTERACTIONS WITH REPORTER
CELLS 47 V. SUMMARY 49 REFERENCES . 49 2. ISOLATION AND PURIFICATION OF
PROTEOGLYCANS JOHN M. WHITELOCK AND RENATO V. IOZZO I. OVERVIEW AND
SCOPE 53 II. EXTRACTION OF PROTEOGLYCANS 55 CONTENTS III. RADIOLABELING
OF PROTEOGLYCANS FOR EASE OF PURIFICATION 57 IV. ANION-EXCHANGE
CHROMATOGRAPHY 57 V. HYDROPHOBIC INTERACTION CHROMATOGRAPHY 59 VI. GEL
FILTRATION CHROMATOGRAPHY 60 VII. IMMUNOAFFINITY CHROMATOGRAPHY 61 VIII.
QUANTIFICATION AND CHARACTERIZATION 63 REFERENCES 66 3. EXPRESSION OF
RECOMBINANT MATRIX COMPONENTS USING BACULOVIRUSES DEANE F. MOSHER,
KRISTIN G. HUWILER, TINA M. MISENHEIMER, AND DOUGLAS S. ANNIS I.
INTRODUCTION: THE IMPORTANCE OF RECOMBINANT EXTRACELLULAR MATRIX
MOLECULES FOR PROTEIN STUDIES 70 II. REQUIREMENTS AND RATIONALE FOR
DEVELOPMENT OF A GENERAL PROTEIN EXPRESSION SYSTEM 70 III. EXPRESSION IN
INSECT CELLS USING PCOCO 74 IV. RESULTS OBTAINED FROM THE COCO SYSTEM 78
REFERENCES 80 4. HEPARAN SULFATE*GROWTH FACTOR INTERACTIONS ALAN C.
RAPRAEGER I. INTRODUCTION: HS REGULATION OF GROWTH FACTOR SIGNALING 83
II. METHODS FOR IDENTIFYING THE ROLE OF HS IN GROWTH FACTOR BINDING AND
SIGNALING 89 III. IDENTIFICATION OF REGULATORY HS 95 IV. ANALYZING THE
STRUCTURE OF HS: GENERAL CONSIDERATIONS AND METHODOLOGIES 102 REFERENCES
104 5. ANALYSIS OF BASEMENT MEMBRANE SELF-ASSEMBLY AND CELLULAR
INTERACTIONS WITH NATIVE AND RECOMBINANT GLYCOPROTEINS PETER D.
YURCHENCO, SERGEI SMIRNOV, AND TODD MATHUS I. INTRODUCTION 111 II.
NATIVE AND RECOMBINANT BASEMENT MEMBRANE PROTEINS 114 III. ANALYSIS OF
SELF-ASSEMBLY IN VITRO 124 IV. ANALYSIS OF CELL-SURFACE CONTRIBUTIONS TO
BASEMENT MEMBRANE ASSEMBLY 135 V APPROACHES TO EVALUATING ECM MOLECULES
FOR ASSEMBLY INTERACTIONS 136 . VI. INTEGRATION OF GENETIC AND MOLECULAR
APPROACHES 137 REFERENCES 138 6. PREPARATION AND ANALYSIS OF SYNTHETIC
MULTICOMPONENT EXTRACELLULAR MATRIX KIM S. MIDWOOD, IWONA
WIERZBICKA-PATYNOWSKI, AND JEAN E. SCHWARZBAUER I. INTRODUCTION TO THE
ECM 145 II. DEVELOPMENT OF SYNTHETIC MATRICES 147 III. DISCUSSION 155
REFERENCES 158 CONTENTS VLL 7. ANALYSIS OF MATRIX DYNAMICS BY ATOMIC
FORCE MICROSCOPY HELEN G. HANSMA, DENNIS O. CLEGG, EFROSINI KOKKOLI,
EMIN OROUDJEV, AND MATTHEW TIRRELL I. INTRODUCTION 164 II. GENERAL
EXPERIMENTAL DESIGN 165 III. PROBE MICROSCOPY IN FLUID: AFM IMAGING 168
IV. PROBE MICROSCOPY IN FLUID: MOLECULAR PULLING/FORCE SPECTROSCOPY 174
V. PROBE MICROSCOPY IN AIR: AFM IMAGING 182 VI. CONCLUSION 188
REFERENCES 188 8. ANALYSIS OF MATRIX DEGRADATION H. G. MUNSHI AND M.
SHARON STACK I. INTRODUCTION 195 II. EXPERIMENTAL APPROACHES AND
PROTOCOLS 196 REFERENCES 204 PART III ADHESION RECEPTOR METHODOLOGIES 9.
EXPRESSION CLONING STRATEGIES FOR THE IDENTIFICATION OF ADHESION
MOLECULES JOE W. RAMOS AND MARK GINSBERG I. INTRODUCTION 209 II.
IMPORTANCE OF METHODOLOGY 212 III. GENERAL CONSIDERATIONS 212 IV.
PROTOCOLS 214 V. POINTS OF VARIATION 218 VI. CRITICAL CONTROLS 219 VII.
POTENTIAL USE IN DISEASE/DRUG SCREENING APPLICATIONS 219 REFERENCES 220
10. PURIFICATION OF INTEGRINS AND CHARACTERIZATION OF
INTEGRIN-ASSOCIATED PROTEINS JOHANNES A. EBLE AND FEDOR BERDITCHEVSKI I.
INTRODUCTION 224 II. ISOLATION OF INTEGRINS: GENERAL CONSIDERATIONS AND
POINTS OF VARIATION 225 III. CHARACTERIZATION OF INTEGRIN-ASSOCIATED
PROTEINS THROUGH MAB PRODUCTION: GENERAL CONSIDERATIONS AND POINTS OF
VARIATION 234 REFERENCES 241 11. METHODS FOR ANALYSIS OF THE INTEGRIN
LIGAND BINDING EVENT JEFFREY W. SMITH I. INTRODUCTION 247 II.
APPLICATION OF AFFINITY CROSS-LINKING TO STUDY OF THE INTEGRIN LIGAND
BINDING POCKET 248 VIII CONTENTS III. MICROTITER-BASED INTEGRIN BINDING
ASSAYS 250 IV. MEASURING LIGAND BINDING TO INTEGRINS WITH SURFACE
PLASMON RESONANCE 254 V. ANALYZING INTEGRIN REDOX STATUS 256 REFERENCES
258 12. INTRACELLULAR COUPLING OF ADHESION RECEPTORS: MOLECULAR
PROXIMITY MEASUREMENTS MADDY PARSONS AND TONY NG I. INTRODUCTION AND
APPLICATIONS 261 II. FLUORESCENT LIFETIME IMAGING MICROSCOPY (FLIM) 265
REFERENCES 276 PART IV FUNCTIONAL APPLICATIONS OF CELL*MATRIX ADHESION
IN MOLECULAR CELL BIOLOGY 13. FUNCTIONAL ANALYSIS OF CELL ADHESION:
QUANTITATION OF CELL-MATRIX ATTACHMENT STEVEN K. AKIYAMA I. INTRODUCTION
281 II. GENERAL CONCEPTS IN CELL ADHESION ASSAYS 283 III. CELL
ATTACHMENT ASSAYS 286 IV. CELL SPREADING ASSAYS 288 V. DIRECT BINDING
ASSAYS 289 VI. CELL MIGRATION ASSAYS 290 VII. CONCLUSIONS 293 REFERENCES
293 14. MEASUREMENTS OF GLYCOSAMINOGLYCAN-BASED CELL INTERACTIONS J.
KEVIN LANGFORD AND RALPH D. SANDERSON I. INTRODUCTION 297 II. CELL
ADHESION ASSAY 299 III. CELL INVASION ASSAY 302 REFERENCES 307 15.
APPLICATIONS OF ADHESION MOLECULE GENE KNOCKOUT CELL LINES JORDAN A.
KREIDBERG I. INTRODUCTION 310 II. DERIVATION OF SOMATIC CELL LINES 311
III. ES CELL LINES CARRYING HOMOZYGOUS MUTATIONS OF INTEGRIN ADHESION
MOLECULE GENES 315 IV. STUDIES WITH ADHESION RECEPTOR NULL CELL LINES
317 V. STUDIES WITH EXTRACELLULAR MATRIX COMPONENT NULL CELL LINES 321
VI. SUMMARY 322 REFERENCES 322 CONTENTS IX 16. FLEXIBLE POLYACRYLAMIDE
SUBSTRATA FOR THE ANALYSIS OF MECHANICAL INTERACTIONS AT CELL*SUBSTRATUM
ADHESIONS KAREN A. BENINGO, CHUN-MIN LA, AND YU-LI WANG I. INTRODUCTION
326 II. PREPARATION OF THE POLYACRYLAMIDE SUBSTRATUM 327 III.
CHARACTERIZATION OF THE GEL 330 IV. DATA COLLECTION FOR THE ANALYSIS OF
TRACTION FORCES 332 V. ANALYSIS OF TRACTION FORCES 334 VI. OTHER
APPLICATIONS USING POLYACRYLAMIDE SUBSTRATA 336 VII. SUMMARY 338
REFERENCES 338 17. CELL MIGRATION IN SLICE CULTURES DONNA J. WEBB,
HANNELORE ASMUSSEN, SHIN-ICHI MURASE, AND ALAN F. HORWITZ I.
INTRODUCTION 341 II. SOMITIC MIGRATION 342 III. MIGRATION FROM THE
SUBVENTRICULAR ZONE TO THE OLFACTORY BULB 349 IV. AXONAL OUTGROWTH 353
V. CONCLUSIONS AND PERSPECTIVES 356 REFERENCES 357 18. APPLICATION OF
CELL ADHESION TO STUDY SIGNALING NETWORKS CINDY K. MIRANTI I.
INTRODUCTION . 359 II. APPLICATIONS 361 III. METHODS 362 REFERENCES 382
19. USE OF MICROPATTERNED ADHESIVE SURFACES FOR CONTROL OF CELL BEHAVIOR
PHILIP LEDUC, EMANUELE OSTUNI, GEORGE WHITESIDES, AND DONALD INGBER I.
INTRODUCTION 385 II. MATERIALS AND INSTRUMENTATION 392 III. PROCEDURES
394 IV. COMMENTS 399 V. PITFALLS 399 REFERENCES 400 20.
ADENOVIRAL-MEDIATED GENE TRANSFER IN TWO-DIMENSIONAL AND
THREE-DIMENSIONAL CULTURES OF MAMMARY EPITHELIAL CELLS HARRIET WATKIN
AND CHARLES H. STREULI I. INTRODUCTION 404 II. USING COLLAGEN I AS A
THREE-DIMENSIONAL MATRIX FOR CELL CULTURE 405 X CONTENTS III. USING
RECONSTITUTED BASEMENT MEMBRANE AS A THREE-DIMENSIONAL MATRIX FOR CELL
CULTURE 407 IV. PREPARING MOUSE MAMMARY EPITHELIAL CELL CULTURES 409 V
PREPARING RECOMBINANT ADENOVIRUS FOR INFECTING MAMMARY EPITHELIAL CELLS
409 VI. INFECTING MAMMARY EPITHELIAL CELL CULTURES WITH ADENOVIRUS 419
REFERENCES 421 PART V GENERAL INFORMATION APPENDIX A: LIST OF SUPPLIERS
427 APPENDIX B: RELEVANT MICROARRAY DATASET EXPERIMENTS 431 APPENDIX C:
WEB SITE RESOURCES OF INTEREST 435 INDEX 441 VOLUMES IN SERIES 455
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| indexdate | 2024-07-09T19:03:44Z |
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| language | English |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Methods in cell-matrix adhesion ed. by Josephine C. Adams Amsterdam [u.a.] Academic Press 2002 XIII, 461 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 69 Methode - Grundsubstanz - Aufsatzsammlung - Zelladhäsion Cell adhesion Extracellular matrix Methode (DE-588)4038971-6 gnd rswk-swf Grundsubstanz (DE-588)4224942-9 gnd rswk-swf Zelladhäsion (DE-588)4314136-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zelladhäsion (DE-588)4314136-5 s Grundsubstanz (DE-588)4224942-9 s Methode (DE-588)4038971-6 s DE-604 Adams, Josephine C. Sonstige (DE-588)1241133441 oth Methods in cell biology 69 (DE-604)BV002534878 69 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009907284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Methods in cell-matrix adhesion Methods in cell biology Methode - Grundsubstanz - Aufsatzsammlung - Zelladhäsion Cell adhesion Extracellular matrix Methode (DE-588)4038971-6 gnd Grundsubstanz (DE-588)4224942-9 gnd Zelladhäsion (DE-588)4314136-5 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4224942-9 (DE-588)4314136-5 (DE-588)4143413-4 |
| title | Methods in cell-matrix adhesion |
| title_auth | Methods in cell-matrix adhesion |
| title_exact_search | Methods in cell-matrix adhesion |
| title_full | Methods in cell-matrix adhesion ed. by Josephine C. Adams |
| title_fullStr | Methods in cell-matrix adhesion ed. by Josephine C. Adams |
| title_full_unstemmed | Methods in cell-matrix adhesion ed. by Josephine C. Adams |
| title_short | Methods in cell-matrix adhesion |
| title_sort | methods in cell matrix adhesion |
| topic | Methode - Grundsubstanz - Aufsatzsammlung - Zelladhäsion Cell adhesion Extracellular matrix Methode (DE-588)4038971-6 gnd Grundsubstanz (DE-588)4224942-9 gnd Zelladhäsion (DE-588)4314136-5 gnd |
| topic_facet | Methode - Grundsubstanz - Aufsatzsammlung - Zelladhäsion Cell adhesion Extracellular matrix Methode Grundsubstanz Zelladhäsion Aufsatzsammlung |
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