Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Kluwer Acad./Plenum Publ.
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references (p. 305-350) and index |
| Beschreibung: | XVIII, 355 S. Ill. |
| ISBN: | 0306467704 |
Internformat
MARC
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| 100 | 1 | |a Hayat, M. A. |d 1936- |e Verfasser |0 (DE-588)129731447 |4 aut | |
| 245 | 1 | 0 | |a Microscopy, immunohistochemistry, and antigen retrieval methods |b for light and electron microscopy |c M. A. Hayat |
| 264 | 1 | |a New York [u.a.] |b Kluwer Acad./Plenum Publ. |c 2002 | |
| 300 | |a XVIII, 355 S. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Includes bibliographical references (p. 305-350) and index | ||
| 650 | 7 | |a Anticorpos (análise) |2 larpcal | |
| 650 | 4 | |a Antigènes | |
| 650 | 7 | |a Antígenos (análise) |2 larpcal | |
| 650 | 4 | |a Immunocytochimie | |
| 650 | 7 | |a Imunohistoquímica (métodos) |2 larpcal | |
| 650 | 7 | |a Microscopia (métodos) |2 larpcal | |
| 650 | 7 | |a Microscopia eletrônica (métodos) |2 larpcal | |
| 650 | 4 | |a Microscopie | |
| 650 | 4 | |a Microscopie électronique | |
| 650 | 4 | |a Antigens | |
| 650 | 4 | |a Antigens |x isolation & purification | |
| 650 | 4 | |a Electron microscopy | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Microscopy | |
| 650 | 0 | 7 | |a Antigen |0 (DE-588)4002272-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroskopie |0 (DE-588)4039238-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Immuncytochemie |0 (DE-588)4161368-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Antigen |0 (DE-588)4002272-9 |D s |
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| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Antigen |0 (DE-588)4002272-9 |D s |
| 689 | 1 | 1 | |a Mikroskopie |0 (DE-588)4039238-7 |D s |
| 689 | 1 | |5 DE-604 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-009855464 | ||
Datensatz im Suchindex
| _version_ | 1804129267573850112 |
|---|---|
| adam_text | Contents
Chapter 1. Introduction 1
Cytogenetic Evaluation of Tumors 11
Genetic Instability of Tumors 12
Tumor Heterogeneity 14
Histological Microdissection 14
Antitumor Vaccines 15
Molecular Genetics 17
cDNA Microarray Technology 18
Angiogenesis 20
Vascular Endothelial Growth Factor 23
Immunohistochemical Localization of Vascular Endothelial
Growth Factor 24
Telepathology (Telemedicine) 25
Future of Immunohistopathology 28
Preparation of Buffers 29
Chapter 2. Antigens and Antibodies 31
Antigens 31
Epitopes 32
Antibodies 33
Polyclonal Antibodies 34
Production of Polyclonal Antiserum 35
Affinity Chromatography 35
Monoclonal Antibodies 37
Specificity of Monoclonal Antibodies 38
MIB 1 Monoclonal Antibody 39
Production of Monoclonal Antibodies 41
Bivalent and Bispecific Monoclonal Antibodies in Cancer Therapy 44
Recombinant Antibodies 46
Anticancer Monoclonal Antibodies 47
xi
xij Contents
Antibody Cross Reactivity 48
Polyreactive Antibodies 49
Commercial Sources of Antibodies 50
Chapter 3. Fixation and Embedding 53
Formaldehyde 53
Nature of Formaldehyde Solution 54
Mechanism of Fixation with Formaldehyde 54
Comparison of Formaldehyde with Glutaraldehyde 56
Fixation with Formaldehyde 57
Effect of Prolonged Fixation with Formaldehyde 58
Formalin Substitute Fixatives 59
Fixation Conditions 60
Effect of Heating on Fixation with Glutaraldehyde 61
Microwave Heat Assisted Fixation with Osmium Tetroxide .... 62
Role of Microwave Heating in Enzyme Cytochemistry 64
Fixation for Enzyme Cytochemistry Using Microwave at Relatively
Low Temperature 64
Cryopreservation in the Presence of Microwave Heating 65
Paraffin Embedding 65
Paraffin Embedding in Microwave Oven 67
Paraffin Embedding in Vacuum Microwave Oven 67
Microtomy of Paraffin Embedded Tissues 67
Silanting of Glass Slides 68
Vacuum Assisted Microwave Heating 69
Chapter 4. Factors Affecting Antigen Retrieval 71
Fixation 71
Denaturation 72
Heating 73
pH 74
Molarity 74
Antigen Retrieval Fluids 75
Glycerin as Antigen Retrieval Fluid 77
Procedure 78
pH of Antigen Retrieval Fluids 78
Ionic Strength of Antigen Retrieval Fluids 79
Antibody Penetration 79
Antibody Dilution 80
Diluent Buffer for Primary Antibodies 82
Storage of Paraffin Embedded Tissues 83
Storage of Tissue Slides 84
Signal Amplification 89
Contents xiii
Tyramine Amplification Method 90
Preparation of Biotinylated Tyramine 92
Rolling Circle Amplification 92
Chapter 5. Problems in Antigen Retrieval 95
Lack of Immunostaining 95
Background Staining 96
Problem of Endogenous Biotin 98
Procedure 100
Mirror Image Complementary Antibodies 101
Procedure 101
Fixation of Frozen Tissues 102
Hot Spots (Areas) in Microwave Oven 102
Problem of Antigen Retrieval Standardization 103
Test Battery 104
Intraobserver and Interobserver Variation in Diagnosis 105
Quantitation of Immunostaining 105
Autostainers 107
Capillary Gap Stainers 109
Centrifugal Stainers 109
Flat Method Stainers 109
Volume Corrected Mitotic Index 110
The Gleason Grading System Ill
Universal Antigen Retrieval Method? 113
Calibration of Microwave Oven 114
Chapter 6. Antigen Retrieval 117
Possible Mechanisms of Antigen Retrieval 117
Nonthermal Effects of Microwave Heating 119
Effect of Endogenous Calcium on Antigen Masking 120
Use of Ethylenediaminetetraacetic Acid (EDTA)
for Antigen Retrieval 123
Antigen Retrieval with Heat Treatment 124
Advantages of Heating 124
Heating Methods 125
Mechanism of Epitope Retrieval by Microwave Heating 130
Duration of Microwave Heating 131
Antigen Retrieval in a High Pressure Microwave Oven 132
Antigen Retrieval at Low Temperature 132
Use of Heat for Staining 136
Rapid Immunostaining of Frozen Sections 138
Enhanced Polymer One Step Staining Procedure 139
Modified En Vision Procedure 139
Hazards and Precautions in the Use of Microwave Ovens 141
xiv Contents
Limitations of Microwave Heating 142
Wet Autoclave Method 145
Procedure 1 145
Procedure 2 146
Ultrasound Treatment 146
Procedure 148
Nonheating Methods 148
Detergents 148
Procedures 149
Proteolytic Enzyme Digestion 151
Procedure 152
Enzyme Digestion and Relatively Low Temperature
(80°C) Assisted Antigen Retrieval 152
Comparison of Antigen Retrieval Methods: A Summary 153
Chapter 7. Antigen Retrieval on Resin Sections 155
Role of Fixative and Embedding Resin in Antigen Retrieval .... 156
Immunostaining of Thin Resin Sections 158
Antigen Retrieval on Sections of Modified Epoxy Resin 160
Effect of Heating 161
Antigen Retrieval on Thin Resin Sections Using Autoclaving ... 161
Rapid Staining of Thin Resin Sections in Microwave Oven 163
Microwave Heat Assisted Rapid Processing of Tissues for Electron
Microscopy 163
Microwave Heat Assisted Immunolabeling of Resin Embedded
Sections 163
Microwave Heat Assisted Immunogold Methods 167
Immunogold Silver Staining 167
Droplet Procedure 167
Chapter 8. General Methods of Antigen Retrieval 169
General Procedure for Antigen Retrieval Using Microwave
Heating 169
Antigen Retrieval in Archival Tissues 173
Method for Microwave Heating of Archival Tissue Blocks 174
Antigen Retrieval Using a Conventional Oven 175
Hot Plate Assisted Antigen Retrieval 175
Procedure 175
Hot Plate Assisted Grading of Vulvar Intraepithelial Neoplasia. . 176
Water Bath Heat Assisted Antigen Retrieval 177
Procedure for Electron Microscopy 178
Procedure for Light Microscopy 178
Procedure for Free Floating Sections 180
Microwave Heat Assisted Evaluation of Global DNA
Hypomethylation 180
Contents xv
Procedure 181
Microwave Heat Assisted Enhanced Peroxidase
One Step Method 181
Procedure 181
Microwave Heat Assisted Immunostaining of Cell Smears 182
Double Immunostaining Using Microwave Heating 182
Microwave Heat Enhanced Double Immunostaining of Nuclear
and Cytoplasmic Antigens 183
Procedure 183
Microwave Heat Assisted Immunohistochemical Localization of
Cyclin Dl 184
Microwave Heat Assisted Immunofluorescence Staining of Tissue
Sections 185
Procedure 186
Microwave Heat Assisted Double Immunofluorescence Labeling 186
Procedure 186
Microwave Heat Assisted Double Indirect Immunofluorescence
Staining 187
Procedure 187
Control Procedures 188
Immunoenzymatic Detection 189
Combined Microwave Heating and Ultrasound Antigen
Retrieval Method 189
Combined Enzyme Digestion and Microwave Heating
Antigen Retrieval Method 190
Pressure Cooker EDTA Assisted Antigen Retrieval 191
2 Mercaptoethanol SodiumIodoacetate Assisted
Antigen Retrieval 191
Antigen Retrieval with Steam EDTA Protease Method 192
Procedure 192
Picric Acid Steam Autoclaving Formic Acid Guanidine
Thiocyanate Assisted Retrieval of Prion Protein 192
Procedure 194
Simultaneous Detection of Multiple Antigens 194
Procedure 196
Use of Multiple Antibodies for Labeling Antigens 196
Procedure 197
Antigen Retrieval in Neuronal Tissue Slices before Vibratome
Sectioning 198
Microwave Heat Assisted Antigen Retrieval in Freshly Frozen
Brain Tissue 198
Procedure 199
Microwave Heat Assisted Rapid Immunostaining of Frozen
Sections 199
Procedure 200
Microwave Heat Assisted Immunocytochemistry of Thin
Cryosections 200
Procedure 201
xvi Contents
Pressure Cooker Assisted Detection of Apoptotic Cells 201
Immunohistochemical Localization of Prostate Specific Antigen 202
Immunohistochemistry 203
Procedure 203
Chapter 9. Other Applications of Microwave Heating 205
Carbohydrate Antigens 205
Ovarian Carcinoma 206
Microwave Heat Assisted Carbohydrate Antigen Retrieval 208
Enzyme Digestion Assisted Carbohydrate Antigen Retrieval 208
Nucleolar Organizer Associated Region Proteins 209
Procedure 211
Nucleolar Size 212
In Situ Hybridization 213
Radioactive Probes 215
Nonradioactive Probes 215
Enhancement of in Situ Hybridization Signal with Microwave Heating 217
Procedure for in Situ Hybridization of DNA 218
In Situ Hybridization of RNA in Skeletal Tissues 219
Microwave Heating for in Situ Hybridization of mRNA in
Plant Tissues 221
Microwave Treatment 221
Staining 221
Microwave Heat Assisted Fluorescence in Situ Hybridization 222
Procedure for Gastrointestinal Neoplasia 222
Nuclear Fluorescence in Situ Hybridization Signal Using
Microwave Heating 223
Microwave Heat Assisted Polymerase Chain Reaction 224
Procedure 224
Detection of Antigens by Flow Cytometry 225
Microwave Heat Assisted Flow Cytometry 225
Procedure 1 227
Procedure 2 228
Microwave Heat Assisted Enzyme Linked Immunosorbent Assay 228
Microwave Heat Assisted Scanning Electron Microscopy 229
Microwave Heat Assisted Confocal Scanning Microscopy .... 230
Microwave Heat Assisted Correlative Microscopy 230
Procedure 230
Chapter 10. Cell Proliferating Antigens 233
Ki 67 Antigen 233
Immunohistochemistry 235
Limitations of Immunohistochemistry 237
Antibodies 237
Contents xvii
Recent Applications of MIB 1 Antibody 239
Ki 67 Antigen Retrieval Using Microwave Heating 240
Ki 67 Antigen Retrieval Using Autoclave Treatment 240
Proliferating Cell Nuclear Antigen 241
Immunohistochemistry 242
Limitations of PCNA Immunohistochemistry 243
Immunostaining of PCNA on Cryostat Sections 245
p53 Antigen 245
Wild Type p53 Protein 247
Mutant p53 Protein 248
p73 249
Antibodies 250
Examples of Antibody Dilutions 253
Immunohistochemistry 253
Use of Multiple Antibodies for Labeling p53 Antigen 256
Wild Type p53 Antigen Retrieval Using Microwave Heating 257
p53 Antigen Retrieval Using Microwave Heating 257
Frozen Section Immunohistochemistry of p53 258
Chapter 11. Estrogens 261
Estrogen Receptors 262
Estrogen Receptor Alpha (ERa) 265
Estrogen Receptor Beta (ERfJ) 267
Estrogen Receptor Gamma (ER7) 268
Distribution of Estrogen Receptors 268
Role of Estrogen Receptors in Breast Cancer 269
Breast Cancer and Tamoxifen 270
Antibodies 270
Immunohistochemistry 273
Comparison of Immunohistochemistry with Biochemical
Ligand Binding Assays 275
Dextran Coated Charcoal Assay 276
Semiquantitative Assessment of Estrogen Receptors 276
Immunostaining of Estrogen Receptors in Prostate Tissue 277
Immunostaining of Estrogen and Progesterone Receptors in
Fine Needle Aspirates of Breast 278
Chapter 12. HER 2 (c erbB 2) Oncoprotein 281
HER 2/neu Oncogene 281
HER 2 Oncoprotein 283
HER 2 Overexpression 284
Simultaneous Overexpression of HER 2 and p53 284
Distribution of HER 2 in Carcinomas 285
xviii Contents
Astrocytic Tumors 285
Bladder Carcinoma 285
Ewing s Sarcoma 285
Intrahepatic Cholangiocellular Carcinoma 286
Laryngeal Squamous Cell Carcinoma 286
Non Small Cell Lung Carcinoma 286
Ovarian Carcinoma 287
Prostate Carcinoma 288
Squamous Cell Carcinoma of Cervix 288
Methods for Detecting HER 2 Status 289
Quantitative Analysis of HER 2/neu Gene Expression 290
Detection of HER 2 Oncoprotein 291
Bispecific Antibodies 293
Bispecific Antibody MDX H210 294
Vaccines 295
Genetic Immunization 295
Immunohistochemistry 296
Herceptin (Trastuzumab) 297
HercepTest 299
Controls and Scoring System 300
Immunostaining of HER 2 Protein Using HercepTest 302
References 305
Index 351
|
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| dewey-ones | 611 - Human anatomy, cytology, histology |
| dewey-raw | 611/.018 |
| dewey-search | 611/.018 |
| dewey-sort | 3611 218 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Book |
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| id | DE-604.BV014403380 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T19:02:25Z |
| institution | BVB |
| isbn | 0306467704 |
| language | English |
| lccn | 2002022128 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009855464 |
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| physical | XVIII, 355 S. Ill. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
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| publisher | Kluwer Acad./Plenum Publ. |
| record_format | marc |
| spelling | Hayat, M. A. 1936- Verfasser (DE-588)129731447 aut Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy M. A. Hayat New York [u.a.] Kluwer Acad./Plenum Publ. 2002 XVIII, 355 S. Ill. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references (p. 305-350) and index Anticorpos (análise) larpcal Antigènes Antígenos (análise) larpcal Immunocytochimie Imunohistoquímica (métodos) larpcal Microscopia (métodos) larpcal Microscopia eletrônica (métodos) larpcal Microscopie Microscopie électronique Antigens Antigens isolation & purification Electron microscopy Immunohistochemistry Microscopy Antigen (DE-588)4002272-9 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Antigen (DE-588)4002272-9 s Immuncytochemie (DE-588)4161368-5 s DE-604 Mikroskopie (DE-588)4039238-7 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009855464&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hayat, M. A. 1936- Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy Anticorpos (análise) larpcal Antigènes Antígenos (análise) larpcal Immunocytochimie Imunohistoquímica (métodos) larpcal Microscopia (métodos) larpcal Microscopia eletrônica (métodos) larpcal Microscopie Microscopie électronique Antigens Antigens isolation & purification Electron microscopy Immunohistochemistry Microscopy Antigen (DE-588)4002272-9 gnd Mikroskopie (DE-588)4039238-7 gnd Immuncytochemie (DE-588)4161368-5 gnd |
| subject_GND | (DE-588)4002272-9 (DE-588)4039238-7 (DE-588)4161368-5 |
| title | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy |
| title_auth | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy |
| title_exact_search | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy |
| title_full | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy M. A. Hayat |
| title_fullStr | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy M. A. Hayat |
| title_full_unstemmed | Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy M. A. Hayat |
| title_short | Microscopy, immunohistochemistry, and antigen retrieval methods |
| title_sort | microscopy immunohistochemistry and antigen retrieval methods for light and electron microscopy |
| title_sub | for light and electron microscopy |
| topic | Anticorpos (análise) larpcal Antigènes Antígenos (análise) larpcal Immunocytochimie Imunohistoquímica (métodos) larpcal Microscopia (métodos) larpcal Microscopia eletrônica (métodos) larpcal Microscopie Microscopie électronique Antigens Antigens isolation & purification Electron microscopy Immunohistochemistry Microscopy Antigen (DE-588)4002272-9 gnd Mikroskopie (DE-588)4039238-7 gnd Immuncytochemie (DE-588)4161368-5 gnd |
| topic_facet | Anticorpos (análise) Antigènes Antígenos (análise) Immunocytochimie Imunohistoquímica (métodos) Microscopia (métodos) Microscopia eletrônica (métodos) Microscopie Microscopie électronique Antigens Antigens isolation & purification Electron microscopy Immunohistochemistry Microscopy Antigen Mikroskopie Immuncytochemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009855464&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hayatma microscopyimmunohistochemistryandantigenretrievalmethodsforlightandelectronmicroscopy |