In situ detection of DNA damage: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2002
|
| Schriftenreihe: | Methods in molecular biology
203 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XVII, 313 S.: Ill., graph. Darst. |
| ISBN: | 0896039528 |
Internformat
MARC
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| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2002 | |
| 300 | |a XVII, 313 S.: Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 203 | |
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| 650 | 7 | |a Técnicas e procedimentos de laboratório |2 larpcal | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Clinical Laboratory Techniques | |
| 650 | 4 | |a DNA Damage | |
| 650 | 4 | |a DNA damage |x Research |x Methodology | |
| 650 | 4 | |a DNA |x analysis | |
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Datensatz im Suchindex
| _version_ | 1804129255355842560 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
Color Plates xvii
Part I. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)
1 Labeling DNA Damage with Terminal Transferase:
Applicability, Specificity, and Limitations
P. Roy Walker, Christine Carson, Julie Leblanc,
and Marianna Sikorska 3
2 TUNEL Assay: An Overview of Techniques
Deryk T. Loo 21
3 Electron Microscopic Detection of DNA Damage Labeled by TUNEL
Antonio Migheli 31
4 Quantitative Differentiation of Both Free 3 OH and 5 OH DNA Ends
Using Terminal Transferase Based Labeling Combined
with Transmission Electron Microscopy
Yoshinori Otsuki and Yuko Ito 41
5 Determination of Three Dimensional Distribution of Apoptotic DNA
Damage by Combination of TUNEL and Quick Freezing
and Deep Etching Techniques
Shinichi Ohno, Takeshi Baba, Nobuo Terada,
and Yasuhisa Fujii 55
6 In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis
by Flow and Laser Scanning Cytometry
Zbigniew Darzynkiewicz, Elzbieta Bedner,
and Piotr Smolewski 69
Part II. Labeling DNA Breaks Using DNA Polymerase I
or its Klenow Fragment
7 DNA Damage Detection Using DNA Polymerase I
or its Klenow Fragment: Applicability, Specificity, Limitations
Jan Hein van Dierendonck 81
8 Labeling DNA Breaks In Situ by Klenow Enzyme
KatherineA. Wood 109
ix
x Contents
9 In Situ Nick Translation at the Electron Microscopic Level
MarcThiry 121
Part III. Labeling DNA Breaks Using Ligase
10 In Situ DNA Ligation as a Method for Labeling Apoptotic Cells
in Tissue Sections: An Overview
Peter J. Hornsby and Vladimir V. Didenko 133
11 Detection of Specific Double Strand DNA Breaks and Apoptosis
In Situ Using T4 DNA Ligase
Vladimir V. Didenko 143
12 In Situ Detection of Double Strand DNA Breaks
with Terminal 5 OH Groups
Vladimir V. Didenko, Hop Ngo, and David S. Baskin 153
Part IV. Detection of DNA Breaks in Agarose Trapped Cells:
Comet Assay and Related Techniques
13 The Comet Assay: Principles, Applications, and Limitations
Andrew R. Collins 163
14 The Comet Assay: An Overview of Techniques
Peggy L. Olive 179
15 Ultrasensitive Detection of DNA Damage
by the Combination of the Comet and TUNEL Assays
Andrei L. Kindzelskii and Howard R. Petty 195
16 Application of FISH to Detect DNA Damage:
DNA Breakage Detection FISH (DBD FISH)
Jose Luis Fernandez and Jaime Gosalvez 203
Part V. Detection of Modified Bases and AP Sites in DNA
17. Simultaneous In Situ Detection of DNA Fragmentation
and RNA/DNA Oxidative Damage Using TUNEL Assay
and Immunohistochemical Labeling
for 8 Hydroxy 2 Deoxyguanosine (8 OHdG)
Alexander E. Kalyuzhny 219
18 The In Situ Detection of Apurinic/Apyrimidinic Sites
and DNA Breaks Bearing Extension Blocking Termini
Philip K. Liu, Jiankun Cui, Niki Moore, and Dongya Huang 235
Part VI. Indirect and General Markers of DNA Damage
19 Markers of Poly (ADP Ribose) Polymerase Activity as Correlates
of DNA Damage
Yinong Zhou, Shi Liang, and Lawrence R. Williams 247
Contents xi
20 Ultrasound Imaging of Apoptosis: DNA Damage Effects Visualized
Gregory J. Czarnota, Michael C. Kolios, John W. Hunt,
and Michael D. Sherar 257
21 p53 Induction as an Indicator of DNA Damage
Galina Selivanova 279
22 Detection of Caspases Activation In Situ by Fluorochrome Labeled
Inhibitors of Caspases (FLICA)
Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski,
Brian W. Lee, and Gary L. Johnson 289
Index 301
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV014380249 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | In situ detection of DNA damage methods and protocols edited by Vladimir V. Didenko Totowa, N.J. Humana Press 2002 XVII, 313 S.: Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 203 Includes bibliographical references and index ADN - Lésions - Recherche - Méthodologie Beschadigingen gtt DNA gtt Detectie gtt Reparação de dna larpcal Técnicas e procedimentos de laboratório larpcal Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Clinical Laboratory Techniques DNA Damage DNA damage Research Methodology DNA analysis Genanalyse (DE-588)4200230-8 gnd rswk-swf Nachweis (DE-588)4115334-0 gnd rswk-swf DNS-Schädigung (DE-588)4150350-8 gnd rswk-swf In situ (DE-588)4293230-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content DNS-Schädigung (DE-588)4150350-8 s Nachweis (DE-588)4115334-0 s In situ (DE-588)4293230-0 s DE-604 Genanalyse (DE-588)4200230-8 s Didenko, Vladimir V. Sonstige oth Methods in molecular biology 203 (DE-604)BV035362695 203 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009847839&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | In situ detection of DNA damage methods and protocols Methods in molecular biology ADN - Lésions - Recherche - Méthodologie Beschadigingen gtt DNA gtt Detectie gtt Reparação de dna larpcal Técnicas e procedimentos de laboratório larpcal Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Clinical Laboratory Techniques DNA Damage DNA damage Research Methodology DNA analysis Genanalyse (DE-588)4200230-8 gnd Nachweis (DE-588)4115334-0 gnd DNS-Schädigung (DE-588)4150350-8 gnd In situ (DE-588)4293230-0 gnd |
| subject_GND | (DE-588)4200230-8 (DE-588)4115334-0 (DE-588)4150350-8 (DE-588)4293230-0 (DE-588)4143413-4 |
| title | In situ detection of DNA damage methods and protocols |
| title_auth | In situ detection of DNA damage methods and protocols |
| title_exact_search | In situ detection of DNA damage methods and protocols |
| title_full | In situ detection of DNA damage methods and protocols edited by Vladimir V. Didenko |
| title_fullStr | In situ detection of DNA damage methods and protocols edited by Vladimir V. Didenko |
| title_full_unstemmed | In situ detection of DNA damage methods and protocols edited by Vladimir V. Didenko |
| title_short | In situ detection of DNA damage |
| title_sort | in situ detection of dna damage methods and protocols |
| title_sub | methods and protocols |
| topic | ADN - Lésions - Recherche - Méthodologie Beschadigingen gtt DNA gtt Detectie gtt Reparação de dna larpcal Técnicas e procedimentos de laboratório larpcal Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Clinical Laboratory Techniques DNA Damage DNA damage Research Methodology DNA analysis Genanalyse (DE-588)4200230-8 gnd Nachweis (DE-588)4115334-0 gnd DNS-Schädigung (DE-588)4150350-8 gnd In situ (DE-588)4293230-0 gnd |
| topic_facet | ADN - Lésions - Recherche - Méthodologie Beschadigingen DNA Detectie Reparação de dna Técnicas e procedimentos de laboratório Wetenschappelijke technieken Wissenschaftliches Arbeiten Clinical Laboratory Techniques DNA Damage DNA damage Research Methodology DNA analysis Genanalyse Nachweis DNS-Schädigung In situ Aufsatzsammlung |
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