Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.):
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Univ., Fak. für Biologie, Diss., 2001 |
| Beschreibung: | 140 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) |c von Alexander Dovzhenko |
| 264 | 1 | |c 2001 | |
| 300 | |a 140 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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Datensatz im Suchindex
| _version_ | 1807864846268497920 |
|---|---|
| adam_text |
CONTENTS
ABBREVIATIONS
.
5
1.
INTRODUCTION
.
6
1.1
PROTOPLAST
CULTURE:
HISTORY
AND
ACHIEVEMENTS
.
6
1.2
RAPESEED:
GENERAL
INFORMATION,
HISTORY
OF
PROTOPLAST
CULTURE
.
9
1.3
SUGARBEET
.
10
1.3.1
SUGARBEET
IS
AN
IMPORTANT
ARABLE
CROP
.
10
1.3.2
TISSUE
CULTURE
.
11
1.3.3
PROTOPLAST
CULTURE
.
13
1.3.4
TRANSFER
OF
FOREIGN
DNA
TO
SUGARBEET
CELLS
.
15
1.4
PLASTID
TRANSFORMATION
OF
HIGHER
PLANTS
.
16
1.5
RESEARCH
AIMS
.
19
2.
MATERIALS
AND
METHODS
.
22
2.1
CHEMICALS
.
22
2.2
BACTERIA
AND
VECTORS
.
23
2.3
PRIMERS
.
24
2.4
METHODS
OF
RECOMBINANT
DNA
AND
VECTOR
CONSTRUCTION
.
24
2.4.1
ISOLATION
OF
PLASMID
DNA
.
25
2.4.2
DEPHOSPHORYLATION
OF
LINEARISED
VECTOR
DNA
.
25
2.4.3
"
BLUNT-ENDING
"
OF
LINEARISED
DNA
AND
"
BLUNT
END
"
AND
"
STICKY
END
"
LIGATION
.
26
2.4.4
TRANSFORMATION
OF
E.COLI
.
26
2.4.5
CLONING
OF
PCR-FRAGMENTS
.
27
2.4.6
CLONING
OF
TRANSFORMATION
VECTORS
WITH
THE
AAR/A-CASSETTE
27
2.5
METHODS
OF
DNA
ANALYSIS
.
27
2.5.1
PCR
(POLYMERASE
CHAIN
REACTION)
.
28
2.5.2
DNA-SEQUENCING
.
29
2.5.3
DNA
ISOLATION
FROM
PLANT
TISSUES
.
29
2.5.4
SOUTHERN
HYBRIDISATION
.
29
2.6
PLANT
MATERIAL
.
30
2.7
MEDIA
AND
SOLUTIONS
.
30
2.8
SEED
STERILISATION
.
33
2.9
SEED
GERMINATION
AND
GROWTH
CONDITIONS
FOR
DONOR
PLANTS
.
34
2.10 CALLUS
INDUCTION
FROM
SUGARBEET
EXPLANTS
AND
ORGANOGENESIS
.
34
2.11
SHOOT
REGENERATION
FROM
SUGARBEET
EXPLANTS
.
36
2.12
EPIDERMAL
PEELINGS
.
36
2.13
PROTOPLAST
ISOLATION,
EMBEDDING
AND
CULTURE
.
36
2.14
PEG
TREATMENT
OF
PROTOPLASTS
.
40
2.15
DNA
TRANSFER
BY
THE
BIOLISTIC
METHOD
.
40
2.16
SELECTION
.
41
2.17
DETECTION
OF
GUS-ACTIVITY
.
42
2.18
COMPUTER
PROGRAMMES
FOR
DNA
ANALYSIS
AND
IMAGE
PROCESSING
42
3.
RESULTS
.
43
3.1
MODEL
SYSTEM:
TOBACCO
PROTOPLAST
CULTURE
.
43
3.1.1
CULTURE
OF
DONOR
PLANTS
.
43
3.1.2
THIN
ALGINATE
LAYER
(TAL)
TECHNIQUE:
A
NOVEL
AND
EFFICIENT
METHOD
FOR
THE
MANIPULATION
OF
PROTOPLASTS
FROM
HIGHER
PLANTS
.
43
3.1.2.1
IMPROVEMENTS
OF
CONDITIONS
FOR
PROTOPLAST
ISOLATION
AND
CULTURE
.
45
3.1.2.2
FAST
SHOOT
FORMATION
FROM
PROTOPLAST
DERIVED
COLONIES
.
47
3.2
RAPESEED
PROTOPLAST
CULTURE
.
48
3
3.2.1
DESIGN
OF
A
NEW
CULTURE
MEDIUM
FOR
RAPESEED
PLANTS
.
48
3.2.2
THE
TAL-TECHNIQUE
AND
A
NEW
CULTURE
MEDIUM
IMPROVED
PROTOPLAST
CULTURE
OF
RAPESEED
.
49
3.2.3
COTYLEDON
PROTOPLASTS
.
51
3.2.4
SHOOT
REGENERATION
FROM
PROTOPLAST
DERIVED
COLONIES
.
52
3.3
SUGARBEET:
SHOOT
REGENERATION
FROM
EXPLANTS
AND
CALLUS
.
53
3.3.1
SEED
GERMINATION
.
53
3.3.2
DIRECT
SHOOT
REGENERATION
.
54
3.3.3
SCREENING
OF
GENOTYPES
FOR
REGENERATION
CAPACITY
.
57
3.3.4
FRIABLE
CALLUS
FORMATION
FROM
OTHER
SOURCES
.
61
3.3.5
CALLUS
INDUCTION
FROM
ETIOLATED
HYPOCOTYL
AND
COTYLEDON
EXPLANTS
.
62
3.3.5.1
REGENERATION
FROM
HYPOCOTYL
CALLUS
.
64
3.4
SUGARBEET
PROTOPLAST
CULTURE
.
65
3.4.1
LEAF
PROTOPLASTS
.
65
3.4.1.1
COMBINATION
OF
GROWTH
CONDITIONS
FOR
DONOR
PLANTS
WITH
STRICT
DIGESTION
PROCEDURE
RESULTS
IN
A
HIGH
YIELD
OF
GUARD
CELL
PROTOPLASTS
.
66
3.4.1.2
PROTOPLAST
CULTURE
AND
REGENERATION
.
67
3.4.2
CALLUS
PROTOPLASTS
.
69
3.4.2.1
PROTOPLAST
CULTURE
.
70
3.4.2.2
SHOOT
REGENERATION
FROM
PROTOPLAST
DERIVED
CALLUS
.
73
3.5
NUCLEAR
TRANSFORMATION
IN
SUGARBEET
.
75
3.5.1
BIALAPHOS
SELECTION
.
76
3.5.2
PEG-MEDIATED
TRANSFORMATION
OF
CALLUS
PROTOPLASTS
IN
SUGARBEET
.
76
3.5.3
BIOLISTIC
TRANSFORMATION
OF
FRIABLE
HYPOCOTYL
CALLUS
.
76
3.5.4
HISTOCHEMICAL
GUS
ANALYSIS
.
78
3.5.5
MOLECULAR
ANALYSIS
.
78
3.6
PLASTID
TRANSFORMATION
OF
RAPESEED
AND
SUGARBEET
.
80
3.6.1
CONSTRUCTION
OF
SPECIES-SPECIFIC
VECTORS
.
80
3.6.2
DETERMINATION
OF
SELECTION
CONDITIONS
.
82
3.6.3
PLASTID
TRANSFORMATION
IN
RAPESEED
.
84
3.6.4
PLASTID
TRANSFORMATION
IN
SUGARBEET
BY
THE
BIOLISTIC
METHOD
.
85
3.6.5
PCR
ANALYSIS
OF
RESISTANT
CELL
LINES
OF
SUGARBEET
.
87
4.
DISCUSSION
.
88
4.1
A
NOVEL,
HIGHLY
EFFICIENT
TECHNIQUE
FOR
PROTOPLAST
CULTURE
.
88
4.2
PROTOPLAST
CULTURE
IN
RAPESEED
.
91
4.2.1
FACTORS
INFLUENCING
PLATING
EFFICIENCIES
.
91
4.2.2
SHOOT
REGENERATION
FROM
PROTOPLAST
DERIVED
COLONIES
.
93
4.3
A
RECALCITRANT
SPECIES
SUGARBEET
(BETA
VULGARIS
L)
.
95
4.3.1
DIRECT
SHOOT
REGENERATION
.
95
4.3.2
REGENERABLE
CALLUS
.
97
4.3.3
PROTOPLASTS
FROM
SUGARBEET
LEAVES
.
99
4.3.4
SHOOT
REGENERATION
FROM
CALLUS
PROTOPLASTS
.
101
4.3.5
NUCLEAR
TRANSFORMATION
.
104
4.4 PLASTID
TRANSFORMATION
IN
RAPESEED
AND
SUGARBEET
.
106
4.5
CONCLUSIONS
AND
PERSPECTIVES
.
110
5.
SUMMARY
.
ILL
6.
REFERENCES
.
112
7.
APPENDIXES
.
129
ACKNOWLEDGEMENTS
.
140 |
| any_adam_object | 1 |
| author | Dovzhenko, Alexander 1974- |
| author_GND | (DE-588)123792207 |
| author_facet | Dovzhenko, Alexander 1974- |
| author_role | aut |
| author_sort | Dovzhenko, Alexander 1974- |
| author_variant | a d ad |
| building | Verbundindex |
| bvnumber | BV014298144 |
| ctrlnum | (OCoLC)50975029 (DE-599)BVBBV014298144 |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV014298144 |
| illustrated | Illustrated |
| indexdate | 2024-08-20T00:37:49Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009807324 |
| oclc_num | 50975029 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-12 |
| owner_facet | DE-19 DE-BY-UBM DE-12 |
| physical | 140 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| record_format | marc |
| spelling | Dovzhenko, Alexander 1974- Verfasser (DE-588)123792207 aut Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) von Alexander Dovzhenko 2001 140 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Fak. für Biologie, Diss., 2001 Hochschulschrift gtt Transformation Genetik (DE-588)4268828-0 gnd rswk-swf Pflanzenzüchtung (DE-588)4045599-3 gnd rswk-swf Zuckerrübe (DE-588)4124906-9 gnd rswk-swf Raps (DE-588)4129767-2 gnd rswk-swf Plastide (DE-588)4149307-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Raps (DE-588)4129767-2 s Pflanzenzüchtung (DE-588)4045599-3 s Plastide (DE-588)4149307-2 s Transformation Genetik (DE-588)4268828-0 s DE-604 Zuckerrübe (DE-588)4124906-9 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009807324&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dovzhenko, Alexander 1974- Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) Hochschulschrift gtt Transformation Genetik (DE-588)4268828-0 gnd Pflanzenzüchtung (DE-588)4045599-3 gnd Zuckerrübe (DE-588)4124906-9 gnd Raps (DE-588)4129767-2 gnd Plastide (DE-588)4149307-2 gnd |
| subject_GND | (DE-588)4268828-0 (DE-588)4045599-3 (DE-588)4124906-9 (DE-588)4129767-2 (DE-588)4149307-2 (DE-588)4113937-9 |
| title | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) |
| title_auth | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) |
| title_exact_search | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) |
| title_full | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) von Alexander Dovzhenko |
| title_fullStr | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) von Alexander Dovzhenko |
| title_full_unstemmed | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) von Alexander Dovzhenko |
| title_short | Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.) |
| title_sort | towards plastid transformation in rapeseed brassica napus l and sugarbeet beta vulgaris l |
| topic | Hochschulschrift gtt Transformation Genetik (DE-588)4268828-0 gnd Pflanzenzüchtung (DE-588)4045599-3 gnd Zuckerrübe (DE-588)4124906-9 gnd Raps (DE-588)4129767-2 gnd Plastide (DE-588)4149307-2 gnd |
| topic_facet | Hochschulschrift Transformation Genetik Pflanzenzüchtung Zuckerrübe Raps Plastide |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009807324&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dovzhenkoalexander towardsplastidtransformationinrapeseedbrassicanapuslandsugarbeetbetavulgarisl |