A new model of choroidal neovascularization:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Freiburg (Breisgau), Univ., Diss., 2001 (Nur beschränkt für den Austausch) |
| Beschreibung: | 83 S. Ill., graph. Darst. : 30 cm |
Internformat
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| 245 | 1 | 0 | |a A new model of choroidal neovascularization |c von Catherine Schwesinger |
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| 650 | 4 | |a Choroidal neovascularization | |
| 650 | 4 | |a Ophthalmology | |
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Datensatz im Suchindex
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CONTENT
PAGE
1. INTRODUCTION 1
1.1. ANATOMY OF THE CHOROID AND THE RETINAL PIGMENT EPITHELIUM 1
1.2. AGE-RELATED MACULAR DEGENERATION 3
1.3. CURRENT THERAPY OF EXSUDATIVE AGE-RELATED MACULAR DEGENERATION 4
1.3.1. MEDICAL TREATMENT 5
1.3.2. SURGICAL TREATMENT 6
1.4. VASCULAR ENDOTHELIAL GROWTH FACTOR 6
1.5. VEGF AND CHOROIDAL NEOVASCULARIZATION 8
1.6. EXPERIMENTAL MODELS OF CHOROIDAL NEOVASCULARIZATION 8
1.7. AIM OF THIS PROJECT 9
2. MATERIALS AND METHODS 10
2.1. MICE 10
2.1.1. CONSTRUCTION OF THE TRANSGENE 10
2.1.2. GENERATION OF FOUNDER MICE WHICH CONSTITUTIVELY OVEREXPRESS VEGF
IN THE RPE 11
2.1.3. VETERINARY CARE 11
2.1.4. EUTHANASIA 11
2.2. EXPRESSION OF THE TRANSGENE IN DIFFERENT MOUSE LINES 12
2.2.1. PURIFICATION OF GENOMIC DNA FROM MOUSE TAILS 12
2.2.2. SOUTHERN BLOT 12
2.2.3. SEQUENCING 14
2.3. PCR TO CONFIRM TRANSGENICITY 16
2.4. DETECTION OF TRANSGENE TRANSCRIPTION 16
2.4.1. REVERSE TRANSCRIPTION-PCR 16
2.4.2.
IN SITU HYBRIDIZATION 17
2.5. DETECTION OF VEGF PROTEIN 19
2.5.1. MOUSE VEGF ELISA 19
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/963565958
2.5.2. IMMUNOHISTOCHEMICAL STAINING FOR VEGF 20
2.6. CHARACTERIZATION OF THE PATHOLOGY 20
2.6.1. EYE SECTIONS 20
2.6.2. H.&E. STAINING AND PAS&HEMATOXYLIN STAINING 21
2.6.3. CD31 STAINING 22
2.6.4. PREPARATION OF CHOROIDAL FLATMOUNTS 23
2.6.5. ADPASE STAINING 24
2.6.6. LECTIN PERFUSION 24
2.6.7. BROMO-DEOXYURIDINE STAINING 25
2.6.8. INDOCYANINE GREEN ANGIOGRAPHY 26
2.6.9. CD 18 STAINING 26
3. RESULTS 28
3.1. THREE TRANSGENIC FOUNDERS WERE GENERATED 28
3.2. TRANSGENE INCORPORATION IN LINE #2 AND #3 28
3.3. SEQUENCING OF THE TRANSGENE IN LINE #2 AND #3 29
3.4. SEPARATING TRANSGENIC MICE FROM CONTROL LITTER MATES 30
3.5. THE MRNA EXPRESSION OF THE TRANSGENE IS AGE-RELATED 31
3.6.
IN SITU HYBRIDIZATION SHOWS VEGF MRNA OVEREXPRESSION IN THE RPE 33
3.7. VEGF PROTEIN LEVELS ARE HIGHER IN TRANSGENIC MICE THAN IN CONTROL
MICE 34
3.7.1. VEGF LEVELS MEASURED BY ELISA 34
3.7.2. VEGF STAINING IN THE CHOROID OF TRANSGENIC MICE 34
3.8. TRANSGENIC MICE DEVELOP INTRACHOROIDAL NEOVASCULARIZATION 35
3.8.1. H.&E. AND PAS&H. STAININGS SHOWS DIFFERENCES IN TRANSGENIC
AND CONTROL EYES 35
3.8.2. CD31 STAINING SHOWS INCREASED CHOROIDAL THICKNESS 37
3.8.3. ADPASE STAINING REVEALS ABNORMAL VESSEL SPROUTS 38
3.8.4. LECTIN PERFUSION SHOWS INTRACHOROIDAL NEOVASCULARIZATION AND
CELLS ADHERENT TO THE VESSEL WALL 40
3.8.5. THE ADHERENT CELLS ARE LEUKOCYTES 41
3.8.6. VESSEL LEAKAGE IS DETECTED BY INDOCYANINE GREEN ANGIOGRAPHY 42
3.8.7. PROLIFERATION OF CHOROIDAL CELLS IS INCREASED 43
4. DISCUSSION
45
4.1. THE EFFECT OF VEGF ON CHOROIDAL VASCULATURE 45
4.1.1. WHAT CAUSES THE CHANGES IN VESSEL MORPHOLOGY? 45
4.1.2. WHY IS THE CHOROIDAL THICKNESS INCREASED? 46
4.1.3. WHY DO THE VESSELS LEAK? 47
4.2. THE ROLE OF INFLAMMATION 48
4.3. THE ROLE OF THE MACULA 49
4.4. THE ROLE OF THE RETINAL PIGMENT EPITHELIUM 51
4.4.1. RPE CELL DENSITY 51
4.4.2. WHAT CAUSES RPE DEGENERATION? 51
4.4.2.1. AGING 51
4.4.2.2. ENZYME ACTIVITY 52
4.4.2.3. DIRECT DAMAGE OF RPE CELLS 53
4.4.2.4. PROTECTING FACTORS 53
4.4.2.5. PHOTORECEPTOR DISEASE 54
4.5. ROLE OF SUBRETINAL NEOVASCULARIZATION 54
4.5.1. THE TRANSGENE WAS EFFECTIVE 54
4.5.2. VEGF EXPRESSION WAS SUFFICIENT 56
4.5.3. ADDITIONAL PATHOMECHANISMS ARE REQUIRED 57
4.5.3.1. BRUCH'S MEMBRANE 57
4.5.3.2. GROWTH FACTORS 58
4.5.3.3. INHIBITORS OF ANGIOGENESIS 59
4.6. A NEW MODEL OF AGE-RELATED MACULAR DEGENERATION? 60
5. SUMMARY 62
6. ZUSAMMENFASSUNG 63
7. REFERENCES 64
8. ACKNOWLEDGMENT 80
9. CURRICULUM VITAE
81 |
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| indexdate | 2024-10-09T18:06:16Z |
| institution | BVB |
| language | English |
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| spelling | Schwesinger, Catherine 1974- Verfasser (DE-588)123474302 aut A new model of choroidal neovascularization von Catherine Schwesinger 2001 83 S. Ill., graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2001 (Nur beschränkt für den Austausch) Age-related macular degeneration Choroidal neovascularization Ophthalmology (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009716296&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schwesinger, Catherine 1974- A new model of choroidal neovascularization Age-related macular degeneration Choroidal neovascularization Ophthalmology |
| subject_GND | (DE-588)4113937-9 |
| title | A new model of choroidal neovascularization |
| title_auth | A new model of choroidal neovascularization |
| title_exact_search | A new model of choroidal neovascularization |
| title_full | A new model of choroidal neovascularization von Catherine Schwesinger |
| title_fullStr | A new model of choroidal neovascularization von Catherine Schwesinger |
| title_full_unstemmed | A new model of choroidal neovascularization von Catherine Schwesinger |
| title_short | A new model of choroidal neovascularization |
| title_sort | a new model of choroidal neovascularization |
| topic | Age-related macular degeneration Choroidal neovascularization Ophthalmology |
| topic_facet | Age-related macular degeneration Choroidal neovascularization Ophthalmology Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009716296&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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