Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Würzburg, Univ., Diss., 2001 |
| Beschreibung: | 106 S. Ill., graph. Darst. : 30 cm |
Internformat
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| 100 | 1 | |a Rieß, Franziska Georgine |d 1972- |e Verfasser |0 (DE-588)123349664 |4 aut | |
| 245 | 1 | 0 | |a Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei |c vorgelegt von Franziska Georgine Rieß |
| 264 | 1 | |c 2000 | |
| 300 | |a 106 S. |b Ill., graph. Darst. : 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Würzburg, Univ., Diss., 2001 | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 650 | 0 | 7 | |a Mycobacterium phlei |0 (DE-588)4667078-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Porin |0 (DE-588)4221584-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroform |0 (DE-588)4039216-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bakterienzellwand |0 (DE-588)4201052-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Nocardia |0 (DE-588)4171964-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 689 | 0 | 1 | |a Bakterienzellwand |0 (DE-588)4201052-4 |D s |
| 689 | 0 | 2 | |a Porin |0 (DE-588)4221584-5 |D s |
| 689 | 0 | 3 | |a Mikroform |0 (DE-588)4039216-8 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 689 | 1 | 0 | |a Mycobacterium phlei |0 (DE-588)4667078-6 |D s |
| 689 | 1 | 1 | |a Bakterienzellwand |0 (DE-588)4201052-4 |D s |
| 689 | 1 | 2 | |a Porin |0 (DE-588)4221584-5 |D s |
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Datensatz im Suchindex
| _version_ | 1812460797314465792 |
|---|---|
| adam_text |
6
CONTENTS
PUBLICATIONS.5
REGULAR PAPERS.5
ABSTRACT.5
CONTENTS.6
CHAPTER 1.10
1.1 INTRODUCTION.10
CHAPTER 2.16
STUDY OF THE PROPERTIES OF A CHANNEL-FORMING PROTEIN OF THE CELL WALL OF
THE
GRAM-POSITIVE BACTERIUM
MYCOBACTERIUM PHLEI.16
2.1 SUMMARY.16
2.2 INTRODUCTION.17
2.3 EXPERIMENTAL PROCEDURES. 18
2.3.1 BACTERIAL STRAIN AND GROWTH CONDITIONS.18
2.3.2 ISOLATION OF THE CHANNEL-FORMING ACTIVITY FROM WHOLE CELLS USING
DETERGENTS.18
2.3.3 SDS-PAGE.19
2.3.4 LIPID BILAYER EXPERIMENTS.19
2.3.5 ESTIMATION OF THE CHANNEL DIAMETER USING THE RENKIN CORRECTION
FACTOR.19
2.3.6 EFFECT OF NEGATIVELY CHARGED GROUPS ATTACHED TO THE CHANNEL
MOUTH.19
2.3.7 SOUTHERN BLOT ANALYSIS. 20
2.4 RESULTS. 20
2.4.1 ISOLATION AND PURIFICATION OF CHANNEL-FORMING PROTEIN OF THE CELL
WALL OF
MYCOBACTERIUM
PHLEI
.20
2.4.2 EFFECT OF THE CELL WALL PROTEIN ON THE CONDUCTANCE OF LIPID
BILAYER MEMBRANES.21
2.4.3 SINGLE CHANNEL ANALYSIS.22
2.4.4 SIZE OF THE CELL WALL CHANNEL.23
2.4.5 SELECTIVITY OF THE CELL WALL PORIN OF
M. PHLEI.24
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/963114751
7
2.4.6 THE CELL WALL CHANNEL (PORIN) OF M. PHLEI IS VOLTAGE-DEPENDENT IN
AN ASYMMETRIC
25
MANNER."
2.4.7 SOUTHERN HYBRIDIZATION OF THE MSPA GENE WITH DNA FROM OTHER
MYCOLATA.27
2.5 DISCUSSION. 28
2.5.1 THE CELL WALL OF
M. PHLEI CONTAINS AN ION-PERMEABLE CHANNEL.28
2.5.2 ESTIMATION OF THE DIAMETER OF THE CELL WALL CHANNEL PROTEIN.29
2.5.3 EFFECT OF POINT CHARGES AT THE CHANNEL MOUTH.I.30
2.5.4 VOLTAGE-CONTROL OF CELL WALL CHANNEL PERMEABILITY.32
2.5.5 CONTAINS THE CHROMOSOME OF M
PHLEI SEVERAL PORIN GENES?.32
2.5.6 IMPLICATIONS OF THE CELL WALL CHANNEL PROPERTIES FOR THE
ANTIBIOTIC RESISTANCE OF
MYCOBACTERIAL CELL WALLS.32
CHAPTER 3.34
THE CELL WALL PORIN OF NOCARDIA FARCINICA: BIOCHEMICAL-IDENTIFICATION OF
THE
CHANNEL-FORMING PROTEIN AND BIOPHYSICAL CHARACTERIZATION OF THE CHANNEL
PROPERTIES.34
3.1 SUMMARY.34
3.2 INTRODUCTION.35
3.3 EXPERIMENTAL PROCEDURES.36
3.3.1 BACTERIAL STRAIN AND GROWTH CONDITIONS.36
3.3.2 ISOLATION OF THE CELL WALLS.37
3.3.3 SDS-PAGE.37
3.3.4 LIPID BILAYER EXPERIMENTS.37
3.3.5 ESTIMATION OF THE CHANNEL DIAMETER.37
3.3.6 EFFECT OF NEGATIVELY CHARGED GROUPS ATTACHED TO THE CHANNEL
MOUTH.38
3.4 RESULTS.39
3.4.1 ISOLATION AND PURIFICATION OF THE CHANNEL-FORMING PROTEIN FROM THE
CELL WALL.39
3.4.2 EFFECT OF THE CELL WALL PROTEIN ON THE CONDUCTANCE OF LIPID
BILAYER MEMBRANES.41
3.4.3 SINGLE CHANNEL ANALYSIS.41
3.4.4 SIZE OF THE CELL WALL CHANNEL.43
3.4.5 EFFECT OF PH ON THE CHANNEL PROPERTIES.44
3.4.6 SELECTIVITY OF THE CELL WALL PORIN OF
N. FARCINICA.44
3.4.7 THE CELL WALL CHANNEL (PORIN) OF
N. FARCINICA IS VOLTAGE-DEPENDENT IN AN ASYMMETRIC
45
MANNER.
3.5 DISCUSSION.48
3.5.1 THE CELL WALL OF
N. FARCINICA CONTAINS A CHANNEL-FORMING PROTEIN.48
8
3.5.2 EVALUATION OF THE DIAMETER OF THE CELL CHANNEL OF
N. FARCINICA
.50
3.5.3 ION SELECTIVITY OF THE
N. FARCINICA
CELL WALL PORIN.51
3.5.4 THE
N. FARCINICA
CELL WALL PORIN IS VOLTAGE-DEPENDENT.52
3.5.5 NEGATIVE POINT CHARGES AT THE CHANNEL MOUTH INFLUENCE THE FLUX OF
CHARGED MOLECULES
THROUGH THE CHANNEL.53
CHAPTER 4.55
THE CELL WALL PORIN OF THE GRAM-POSITIVE BACTERIUM NOCARDIA ASTEROIDES
FORMS
CATION-SELECTIVE CHANNELS THAT EXHIBIT ASYMMETRIC VOLTAGE-DEPENDENCE
.55
4.1 SUMMARY.55
4.2 INTRODUCTION.56
4.3 MATERIALS AND METHODS.58
4.3.1 BACTERIAL STRAIN AND GROWTH CONDITIONS.58
4.3.2 ISOLATION OF THE CELL WALLS.58
4.3.3 ISOLATION OF THE CHANNEL-FORMING PROTEIN.59
4.3.4 SDS-PAGE.59
4.3.5 RECONSTITUTION EXPERIMENTS WITH LIPID BILAYER MEMBRANES.59
4.3.6 ESTIMATION OF THE CHANNEL DIAMETER USING THE RENKIN CORRECTION
FACTOR.60
4.3.7 CHANNEL SIZE ESTIMATED FROM THE EFFECT OF NEGATIVELY CHARGED
GROUPS AT THE CHANNEL. 61
4.4 RESULTS.61
4.4.1 IDENTIFICATION AND PURIFICATION OF THE CHANNEL-FORMING PROTEIN
FROM THE CELL WALL OF
N. ASTEROIDES.
. 61
4.4.2 THE
N. ASTEROIDES
CELL WALL PORIN FORMS ION-PERMEABLE CHANNELS.63
4.4.3 ZERO-CURRENT MEMBRANE POTENTIAL MEASUREMENTS.66
4.4.4 VOLTAGE DEPENDENCE.66
4.5 DISCUSSION.68
4.5.1 THE CELL WALL OF
NOCARDIA ASTEROIDES
CONTAINS A CHANNEL-FORMING PROTEIN.69
4.5.2 THE CELL WALL CHANNEL OF
N. ASTEROIDES
IS CATION-SELECTIVE.69
4.5.3 THE
N. ASTEROIDES
CELL WALL CHANNEL IS VOLTAGE-GATED.71
4.5.4 ESTIMATION OF THE DIAMETER USING THE RENKIN CORRECTION FACTOR.72
CHAPTERS.74
DISCOVERY OF A NOVEL CHANNEL-FORMING PROTEIN IN THE CELL WALL OF THE
NON-
PATHOGENIC
NOCARDIA CORYNEBACTEROIDES
.74
5.1 SUMMARY.74
5.2 INTRODUCTION.75
5.3 MATERIALS AND METHODS.76
9
5.3.1 BACTERIAL STRAIN AND GROWTH CONDITIONS.76
5.3.2 ISOLATION OF THE CHANNEL-FORMING ACTIVITY FROM WHOLE CELLS USING
DETERGENTS.76
5.3.3 2-D GEL ELECTROPHORESIS.77
5.3.4 SDS-PAGE.77
5.3.5 RECONSTITUTION EXPERIMENTS WITH LIPID BILAYER MEMBRANES.77
5.3.6 ESTIMATION OF THE CHANNEL DIAMETER USING THE RENKIN CORRECTION
FACTOR.77
5.3.7 EFFECT OF NEGATIVELY CHARGED GROUPS ATTACHED TO THE CHANNEL
MOUTH.78
5.4 RESULTS. 78
5.4.1 ISOLATION, PURIFICATION AND PI OF THE CHANNEL-FORMING PROTEIN FROM
THE CELL WALL.78
5.4.2 EFFECT OF THE CELL WALL PROTEIN ON THE CONDUCTANCE OF LIPID
BILAYER MEMBRANES.79
5.4.3 SINGLE CHANNEL ANALYSIS.80
5.4.4 SIZE OF THE CELL WALL CHANNEL.81
5.4.5 SELECTIVITY OF THE CELL WALL PORIN OF
N.CORYNEBACTEROIDES.82
5.4.6 THE CELL WALL CHANNEL PORIN OF
N.CORYNEBACTEROIDES IS VOLTAGE-DEPENDENT IN A DEFINED
ASYMMETRIC MANNER.83
5.5 DISCUSSION.85
5.5.1 THE CELL WALL OF
N.CORYNEBACTEROIDES CONTAINS A CHANNEL-FORMING PROTEIN.85
5.5.2 EVALUATION OF THE DIAMETER OF THE CELL WALL CHANNEL OF
N.CORYNEBACTEROIDES.86
5.5.3 ION SELECTIVITY OF THE
N.CORYNEBACTEROIDES CELL WALL PORIN.87
5.5.4 THE
N.CORYNEBACTEROIDES CELL WALL PORIN IS VOLTAGE-DEPENDENT.88
5.5.5 IMPLICATIONS FOR THE PERMEABILITY OF THE CELL WALL.89
CHAPTER 6.*.91
SUMMARY.
91
6.1 SUMMARY.91
6.2 ZUSAMMENFASSUNG. 94
CHAPTER 7. 97
APPENDIX. 97
7.1 REFERENCES.97
7.2 CURRICULUM VITAE.105
ACKNOWLEDGMENTS.106 |
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| id | DE-604.BV014173967 |
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| indexdate | 2024-10-09T18:08:31Z |
| institution | BVB |
| language | English |
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| physical | 106 S. Ill., graph. Darst. : 30 cm |
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| spelling | Rieß, Franziska Georgine 1972- Verfasser (DE-588)123349664 aut Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei vorgelegt von Franziska Georgine Rieß 2000 106 S. Ill., graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2001 Hochschulschrift gtt Mycobacterium phlei (DE-588)4667078-6 gnd rswk-swf Porin (DE-588)4221584-5 gnd rswk-swf Mikroform (DE-588)4039216-8 gnd rswk-swf Bakterienzellwand (DE-588)4201052-4 gnd rswk-swf Nocardia (DE-588)4171964-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Nocardia (DE-588)4171964-5 s Bakterienzellwand (DE-588)4201052-4 s Porin (DE-588)4221584-5 s Mikroform (DE-588)4039216-8 s 1\p DE-604 Mycobacterium phlei (DE-588)4667078-6 s 2\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009716121&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Rieß, Franziska Georgine 1972- Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei Hochschulschrift gtt Mycobacterium phlei (DE-588)4667078-6 gnd Porin (DE-588)4221584-5 gnd Mikroform (DE-588)4039216-8 gnd Bakterienzellwand (DE-588)4201052-4 gnd Nocardia (DE-588)4171964-5 gnd |
| subject_GND | (DE-588)4667078-6 (DE-588)4221584-5 (DE-588)4039216-8 (DE-588)4201052-4 (DE-588)4171964-5 (DE-588)4113937-9 |
| title | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei |
| title_auth | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei |
| title_exact_search | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei |
| title_full | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei vorgelegt von Franziska Georgine Rieß |
| title_fullStr | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei vorgelegt von Franziska Georgine Rieß |
| title_full_unstemmed | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei vorgelegt von Franziska Georgine Rieß |
| title_short | Isolation and characterization of channel forming proteins in the cell wall of three different Nocardia species and Mycobacterium phlei |
| title_sort | isolation and characterization of channel forming proteins in the cell wall of three different nocardia species and mycobacterium phlei |
| topic | Hochschulschrift gtt Mycobacterium phlei (DE-588)4667078-6 gnd Porin (DE-588)4221584-5 gnd Mikroform (DE-588)4039216-8 gnd Bakterienzellwand (DE-588)4201052-4 gnd Nocardia (DE-588)4171964-5 gnd |
| topic_facet | Hochschulschrift Mycobacterium phlei Porin Mikroform Bakterienzellwand Nocardia |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009716121&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rießfranziskageorgine isolationandcharacterizationofchannelformingproteinsinthecellwallofthreedifferentnocardiaspeciesandmycobacteriumphlei |