Enzyme kinetics: principles and methods
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English German |
| Veröffentlicht: |
Weinheim ; New York ; Chichester ; Brisbane ; Singapore
WILEY-VCH
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 255 S. Ill., graph. Darst. : 24 cm 1 CD-ROM (12 cm) |
| ISBN: | 352730343X |
Internformat
MARC
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| 240 | 1 | 0 | |a Enzymkinetik |
| 245 | 1 | 0 | |a Enzyme kinetics |b principles and methods |c Hans Bisswanger. Transl.: Leonie Bubenheim |
| 264 | 1 | |a Weinheim ; New York ; Chichester ; Brisbane ; Singapore |b WILEY-VCH |c 2002 | |
| 300 | |a XIV, 255 S. |b Ill., graph. Darst. : 24 cm |e 1 CD-ROM (12 cm) | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Enzyme kinetics | |
| 650 | 4 | |a Enzyme kinetics |v Software | |
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Datensatz im Suchindex
| _version_ | 1804129037216382976 |
|---|---|
| adam_text | CONTENTS
SYMBOLS AND ABBREVIATIONS XIII
INTRODUCTION 1
1 MULTIPLE EQUILIBRIA 5
1.1 DIFFUSION 5
1.2 INTERACTION OF LIGANDS AND MACROMOLECULES 10
1.2.1 BINDING CONSTANTS 10
1.2.2 DERIVATION OF THE BINDING EQUATION 11
1.3 MACROMOLECULES WITH IDENTICAL INDEPENDENT BINDING SITES 11
1.3.1 GENERAL BINDING EQUATION 11
1.3.2 GRAPHIC REPRESENTATION OF THE GENERAL BINDING EQUATION 17
1.3.3 BINDING OF VARIOUS LIGANDS, COMPETITION 22
1.4 MACROMOLECULES WITH NON-IDENTICAL, INDEPENDENT
BINDING SITES 26
1.5 MACROMOLECULES WITH IDENTICAL, INTERACTING BINDING SITES,
COOPERATIVITY 28
1.5.1 THE HILL EQUATION 28
1.5.2 THE ADAIR EQUATION 30
1.5.3 THE PAULING MODEL 32
1.5.4 ALLOSTERIC ENZYMES 32
1.5.5 THE SYMMETRY MODEL 33
1.5.6 THE SEQUENTIAL MODEL AND NEGATIVE COOPERATIVITY 38
1.5.7 PHYSIOLOGICAL ASPECTS OF COOPERATIVITY 41
1.5.8 ANALYSIS OF COOPERATIVITY 44
1.5.9 EXAMPLES OF ALLOSTERIC ENZYMES 45
1.6 NON-IDENTICAL, INTERACTING BINDING SITES 48
1.7 REFERENCES 49
2 ENZYME KINETICS 51
2.1 REACTION ORDER 51
2.1.1 FIRST ORDER REACTIONS 51
2.1.2 SECOND ORDER REACTIONS 53
2.1.3 ZERO ORDER REACTIONS 54
X CONTENTS
2.2 STEADY-STATE KINETICS AND THE MICHAELIS-MENTEN EQUATION 55
2.2.1 DERIVATION OF THE MICHAELIS-MENTEN EQUATION 55
2.3 ANALYSIS OF ENZYME KINETIC DATA 58
2.3.1 GRAPHICAL REPRESENTATIONS OF THE MICHAELIS-MENTEN EQUATION 58
2.3.2 DETERMINATION OF THE REACTION RATE 71
2.4 REVERSIBLE ENZYME REACTIONS 75
2.4.1 RATE EQUATION FOR REVERSIBLE ENZYME REACTIONS 75
2.4.2 THE HALDANE EQUATION 77
2.4.3 PRODUCT INHIBITION 78
2.5 ENZYME INHIBITION 80
2.5.1 REVERSIBLE ENZYME INHIBITION 81
2.5.2 IRREVERSIBLE ENZYME INHIBITION 103
2.5.3 ENZYME REACTIONS WITH TWO COMPETING SUBSTRATES 106
2.6 MULTI-SUBSTRATE REACTIONS 108
2.6.1 NOMENCLATURE 108
2.6.2 RANDOM MECHANISM 109
2.6.3 ORDERED MECHANISM 113
2.6.4 PING-PONG MECHANISM 115
2.6.5 HALDANE RELATIONSHIPS IN MULTI-SUBSTRATE REACTIONS 117
2.6.6 MECHANISMS WITH MORE THAN TWO SUBSTRATES 118
2.6.7 OTHER NOTATIONS FOR MULTI-SUBSTRATE REACTIONS 120
2.7 DERIVATION OF RATE EQUATIONS OF COMPLEX ENZYME
MECHANISMS 120
2.7.1 KING-ALTMAN METHOD 120
2.7.2 SIMPLIFIED DERIVATIONS ACCORDING TO THE GRAPH THEORY 126
2.7.3 COMBINATION OF EQUILIBRIUM AND STEADY-STATE ASSUMPTIONS 127
2.8 KINETIC TREATMENT OF ALLOSTERIC ENZYMES 129
2.8.1 HYSTERETIC ENZYMES 129
2.8.2 KINETIC COOPERATIVITY, THE SLOW TRANSITION MODEL 130
2.9 SPECIAL ENZYME MECHANISMS 131
2.9.1 KINETICS OF IMMOBILISED ENZYMES 131
2.9.2 POLYMER SUBSTRATES 138
2.10 PH AND TEMPERATURE DEPENDENCE OF ENZYMES 139
2.10.1 PH OPTIMUM CURVE AND DETERMINATION OF PK VALUES 139
2.10.2 PH STABILITY OF ENZYMES 141
2.10.3 THERMAL STABILITY OF ENZYMES 142
2.10.4 TEMPERATURE DEPENDENCE OF ENZYME REACTIONS 143
2.11 ISOTOPE EXCHANGE 146
2.11.1 ISOTOPE EXCHANGE KINETICS 146
2.11.2 ISOTOPE EFFECTS 150
CONTENTS XI
2.12 APPLICATION OF STATISTICAL METHODS IN ENZYME KINETICS 153
2.12.1 GENERAL REMARKS 153
2.12.2 STATISTICAL TERMS USED IN ENZYME KINETICS 156
2.13 REFERENCES 158
3 METHODS 161
3.1 METHODS FOR THE INVESTIGATION OF MULTIPLE EQUILIBRIA 161
3.1.1 EQUILIBRIUM DIALYSIS AND GENERAL ASPECTS
OF BINDING MEASUREMENTS 162
3.1.2 CONTINUOUS EQUILIBRIUM DIALYSIS 168
3.1.3 ULTRAFILTRATION 170
3.1.4 GEL FILTRATION 172
3.1.5 ULTRACENTRIFUGATION METHODS 175
3.2 ELECTROCHEMICAL METHODS 180
3.2.1 THE OXYGEN ELECTRODE 181
3.2.2 THE CO
2
ELECTRODE 183
3.2.3 POTENTIOMETRY, OXIDATION-REDUCTION POTENTIALS 183
3.2.4 THEPH-STAT 184
3.2.5 POLAROGRAPHY 185
3.3 CALORIMETRY 186
3.4 SPECTROSCOPIC METHODS 188
3.4.1 ABSORPTION SPECTROSCOPY 190
3.4.2 BIOLUMINESCENCE 201
3.4.3 FLUORESCENCE 201
3.4.4 CIRCULAR DICHROISM AND OPTICAL ROTATION DISPERSION 212
3.4.5 INFRARED AND RAMAN SPECTROSCOPY 217
3.4.6 ELECTRON SPIN RESONANCE SPECTROSCOPY 219
3.5 MEASUREMENT OF FAST REACTIONS 222
3.5.1 FLOW METHODS 223
3.5.2 RELAXATION METHODS 231
3.5.3 FLASH PHOTOLYSIS, PICO- AND FEMTOSECONDS SPECTROSCOPY 236
3.5.4 EVALUATION OF RAPID KINETIC REACTIONS (TRANSIENT KINETICS) 238
3.6 REFERENCES 241
INDEX 247
|
| any_adam_object | 1 |
| author | Bisswanger, Hans 1943- |
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| author_facet | Bisswanger, Hans 1943- |
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| author_sort | Bisswanger, Hans 1943- |
| author_variant | h b hb |
| building | Verbundindex |
| bvnumber | BV014160578 |
| classification_rvk | WC 4350 WD 5050 |
| classification_tum | CHE 827f |
| ctrlnum | (OCoLC)47812624 (DE-599)BVBBV014160578 |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 547 - Organic chemistry 572 - Biochemistry |
| dewey-raw | 547.75804594 572.744 |
| dewey-search | 547.75804594 572.744 |
| dewey-sort | 3547.75804594 |
| dewey-tens | 540 - Chemistry and allied sciences 570 - Biology |
| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| id | DE-604.BV014160578 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:58:46Z |
| institution | BVB |
| isbn | 352730343X |
| language | English German |
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| physical | XIV, 255 S. Ill., graph. Darst. : 24 cm 1 CD-ROM (12 cm) |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | WILEY-VCH |
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| spelling | Bisswanger, Hans 1943- Verfasser (DE-588)129060461 aut Enzymkinetik Enzyme kinetics principles and methods Hans Bisswanger. Transl.: Leonie Bubenheim Weinheim ; New York ; Chichester ; Brisbane ; Singapore WILEY-VCH 2002 XIV, 255 S. Ill., graph. Darst. : 24 cm 1 CD-ROM (12 cm) txt rdacontent n rdamedia nc rdacarrier Enzyme kinetics Enzyme kinetics Software Enzymkinetik (DE-588)4133166-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Enzymkinetik (DE-588)4133166-7 s Methode (DE-588)4038971-6 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009705996&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Bisswanger, Hans 1943- Enzyme kinetics principles and methods Enzyme kinetics Enzyme kinetics Software Enzymkinetik (DE-588)4133166-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4133166-7 (DE-588)4038971-6 |
| title | Enzyme kinetics principles and methods |
| title_alt | Enzymkinetik |
| title_auth | Enzyme kinetics principles and methods |
| title_exact_search | Enzyme kinetics principles and methods |
| title_full | Enzyme kinetics principles and methods Hans Bisswanger. Transl.: Leonie Bubenheim |
| title_fullStr | Enzyme kinetics principles and methods Hans Bisswanger. Transl.: Leonie Bubenheim |
| title_full_unstemmed | Enzyme kinetics principles and methods Hans Bisswanger. Transl.: Leonie Bubenheim |
| title_short | Enzyme kinetics |
| title_sort | enzyme kinetics principles and methods |
| title_sub | principles and methods |
| topic | Enzyme kinetics Enzyme kinetics Software Enzymkinetik (DE-588)4133166-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Enzyme kinetics Enzyme kinetics Software Enzymkinetik Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009705996&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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