Fixed-bed reactors for the cultivation of animal cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Düsseldorf
VDI-Verl.
2001
|
| Ausgabe: | Als Ms. gedr. |
| Schriftenreihe: | Fortschrittberichte VDI
Reihe 17, Biotechnik ; 209 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Hamburg-Harburg, Techn. Univ., Diss., 2000 |
| Beschreibung: | XIV, 157 S. Ill., graph. Darst. : 21 cm |
| ISBN: | 3183209179 |
Internformat
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| 245 | 1 | 0 | |a Fixed-bed reactors for the cultivation of animal cells |c Dieter Fassnacht |
| 250 | |a Als Ms. gedr. | ||
| 264 | 1 | |a Düsseldorf |b VDI-Verl. |c 2001 | |
| 300 | |a XIV, 157 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Fortschrittberichte VDI : Reihe 17, Biotechnik |v 209 | |
| 500 | |a Zugl.: Hamburg-Harburg, Techn. Univ., Diss., 2000 | ||
| 650 | 4 | |a Bioreactors | |
| 650 | 4 | |a Biotechnology |x instrumentation | |
| 650 | 4 | |a Biotechnology |x methods | |
| 650 | 4 | |a Cell Culture Techniques |x methods | |
| 650 | 4 | |a Genetic Engineering |x methods | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009698804 | |
Datensatz im Suchindex
| _version_ | 1816444708048601088 |
|---|---|
| adam_text |
C
ONTENTS
V
1
OBJECTIVE
TARGET
.
1
2
INTRODUCTION
.
3
2.1
ANIMAL
CELL
TECHNOLOGY
.
3
2.1.1
CHARACTERISTICS
OF
ANIMAL
CELLS
.
3
2.1.2
DEVELOPMENTS
IN
ANIMAL
CELL
TECHNOLOGY
.
4
2.1.3
CELL
CULTURE,
MICROORGANISMS
OR
TRANSGENIC
ANIMALS?
.
5
2.2
FIXED-BED
REACTORS
.
6
2.2.1
CARRIERS
.
9
2.3
APOPTOSIS
.
12
3
MATERIALS
AND
METHODS
.
13
3.1
CELL
LINES
AND
FLASK
CULTIVATION
.
13
3.1.1
HYBRIDOMA
CELL
LINES
.
13
3.1.1.1
IV
F
19.23
.13
3.1.1.2
TB/C3,
TB/C3-BCL2
AND
TB/C3-PEF
.
14
3.1.2
CH0-MG12
.
14
3.1.3
IMMORTALIZED
HEPATOCYTES
.
16
3.2
MEDIA
.
16
3.2.1
BASAL
MEDIA
.
16
3.2.2
MEDIUM
SUPPLEMENTS
.
17
3.2.2.1
GROWTH
SUPPLEMENTS
.
17
3.2.2.2
ANTIBIOTICS
.
17
3.3
ANALYTICAL
METHODS
.
17
3.3.1
SAMPLE
PREPARATION
AND
STORAGE
.
17
3.3.2
CELL
DENSITY
.
18
3.3.2.1
TRYPAN
BLUE
EXCLUSION
.
18
3.3.2.2
ACRIDINE
ORANGE,
PROPIDIUM
IODINE
STAINING
.
18
3.3.2.3
CRYSTAL
VIOLET
STAINING
.
19
3.3.3
GLUCOSE
AND
LACTATE
.
19
3.3.4
GLUTAMINE,
AMMONIA,
UREA
.
20
3.3.5
DNA
IN
THE
SUPERNATANT
.
20
3.3.6
DNA
GEL
ELECTROPHORESIS
.
20
3.3.7
SEAP
(HUMAN
PLACENTAL
ALKALINE
PHOSPHATASE)
.21
3.3.8
MONOCLONAL
ANTIBODIES
.
21
3.3.9
AMINO
ACIDS
.
22
VI
_
C
ONTENTS
3.3.10
SCANNING
ELECTRON
MICROSCOPY
.
22
3.4
FIXED-BED
REACTOR*
.
22
3.4.1
CARRIER
MATERIAL
.
24
3.4.2
MASS-BALANCE
.
26
3.4.3
SMALL-SCALE
REACTOR
(10
ML
AXIAL-FLOW
FIXED-BED)
.
27
3.4.4
40
ML
AXIAL-FLOW
FIXED-BED
REACTOR
.
28
3.4.5
LABORATORY
SCALE
REACTOR
(100
ML
AXIAL-FLOW
FIXED-BED)
.
29
4
INCREASING
PRODUCTIVITY
OF
FIXED-BED
SYSTEMS
.
31
4.1
SUPPLEMENTING
MEDIUM
WITH
PMEG
TO
REDUCE
PROLIFERATION
.
32
4.1.1
BATCH
CULTIVATION
.
33
4.1.2
FIXED-BED
EXPERIMENTS
.
35
4.1.3
CONCLUSION
.
36
4.2
GENETIC
ENGINEERING
OF
CELLS
TO
INHIBIT
APOPTOSIS
.
37
4.2.1
HYBRIDOMA
CELL
LINES
TB/C3-PEF
AND
TB/C3-BCL2
.
37
4.2.1.1
COMPARISON
OF
BOTH
CELL
LINES
IN
BATCH
EXPERIMENTS
.
37
4.2.1.2
CELL-CYCLE
MODEL
OF
CHEMOSTAT
SUSPENSION
CULTURES
.
43
4.2.1.3
CONTINUOUS
HIGH
DENSITY
CULTURES
.
50
4.2.1.4
DISCUSSION
.
57
4.2.2
CHO-MG12
*
.
59
4.2.2.1
BATCH
CULTIVATION
OF
CHO-MG12
.
60
4.2.2.2
FIXED-BED
CULTIVATION
OF
CHO-MG12
.
64
4.2.2.3
DISCUSSION
.
66
4.3
FIXED-BED
CULTURES
AT
ELEVATED
OXYGEN
CONCENTRATIONS
.
67
4.3.1
TIME
COURSE
OF
A
LONG-TERM
EXPERIMENT
.
67
4.3.2
COMPARISON
OF
FIXED-BED
EXPERIMENTS
USING
VARIOUS
OXYGEN
CONCENTRATIONS
.
70
5
NOVEL
APPLICATIONS
FOR
FIXED-BED
REACTORS
.72
5.1
TOWARDS
AN
ARTIFICIAL
LIVER
ASSIST
DEVICE
.
72
5.1.1
CULTIVATION
OF
IMMORTALIZED
HEPATOCYTES
.
73
5.1.1.1
BATCH
CULTIVATION
.
73
5.1.1.2
GROWTH
IN
THE
SMALL-SCALE
REACTOR
.
74
5.1.1.3
LONG-TERM
CULTIVATION
IN
THE
40
ML
FIXED-BED
REACTOR
.
76
5.1.2
SCALE-UP
CONSIDERATIONS
.
79
5.1.3
DISCUSSION
.
79
5.2
FIXED-BED
REACTORS
FOR
NMR
TECHNOLOGY
.
81
5.2.1
NMR
METHODS
.
81
C
ONTENTS
VII
5.2.2
CULTIVATION
.
82
5.2.3
RESULTS
.
82
6
MODEL
FOR
FIXED-BED
REACTORS
.
85
6.1
REACTION-DIFFUSION
MODEL
.
86
6.1.1
REACTION-DIFFUSION
LIMITATION
WITHIN
THE
CARRIER
.
87
6.1.2
BOUNDARY
LAYER
.
89
6.1.3
INTRAPARTICLE
CONVECTION
.
90
6.1.4
CONCENTRATION
PROFILE
OVER
THE
ENTIRE
FIXED-BED
.91
6.1.4.1
AXIAL-FLOW
FIXED-BED
.91
6.1.4.2
RADIAL-FLOW
FIXED-BED
.
92
6.1.5
MASS
BALANCE
EQUATIONS
.
93
6.2
MODEL
OF
OXYGEN
LIMITATION
FOR
A
HYBRIDOMA
CELL
LINE
.
94
6.2.1
PARAMETERS
OF
THE
MODEL
.
95
6.2.2
INFLUENCE
OF
THE
SUPERFICIAL
FLOW
VELOCITY
ON
THE
PERFORMANCE
.
95
6.2.3
INFLUENCE
OF
THE
DISSOLVED
OXYGEN
CONCENTRATION
ON
THE
PERFORMANCE
.
96
6.2.4
INFLUENCE
OF
THE
CARRIER
RADIUS
ON
THE
PERFORMANCE
.
99
6.3
MODELING
OF
GLUCOSE
AND
GLUTAMINE
LIMITATION
.
100
6.4
CONCLUAIONS
.
102
7
PROCESS
SCALE-UP
.
103
7.1
FLOW
CHARACTERISTICS
OF
A
13
LITER
RADIAL-FLOW
FIXED-BED
.
104
7.1.1
DISPERSION
IN
RADIAL-FLOW
FIXED-BEDS
.
104
7.1.1.1
DYNAMIC
MASS
BALANCE
OF
RADIAL-FLOW
PACKED
BEDS
.
106
7.1.2
CONSTRUCTION
AND
EXPERIMENTAL
SET-UP
OF
A
FIXED-BED
FOR
RTD
EXPERIMENTS
.
109
7.1.3
RESULTS
OF
THE
RESIDENCE
TIME
DISTRIBUTION
EXPERIMENTS
.
110
7.1.4
CONSIDERATION
FOR
SCALE-UP
.111
7.1.4.1
PRESSURE
DROP
OF
RADIAL-FLOW
FIXED-BEDS
.
111
7.2
PROTOTYPE
RADIAL-FLOW
REACTOR
(1.5
LITER
FIXED-BED)
.
114
7.2.1
DESIGN
AND
CONSTRUCTION
.
114
7.2.1.1
FINDING
THE
OPTIMAL
FIXED-BED
DIMENSIONS
.
114
7.2.1.2
CONSTRUCTION
.
117
7.2.1.3
INOCULATION
DURING
CULTIVATION
.
119
7.2.2
CULTIVATION
OF
THE
HYBRIDOMA
CELL
LINE
IV
F
19.23
.
120
7.2.3
DISCUSSION
.
123
7.3
LARGE-SCALE
FIXED-BED
REACTOR*
.
123
VIII
C
ONTENTS
7.3.1
FIXED-BED
FOR
A
19
LITER
STIRRED-TANK
BIOREACTOR
(4.3
LITER
FIXED-BED
VOLUME)
.
124
7.3.1.1
DESIGN
AND
CONSTRUCTION
.
124
7.3.2
LARGE-SCALE
REACTOR
(5.6
LITER
FIXED-BED
VOLUME)
.
126
7.3.2.1
DESIGN
AND
CONSTRUCTION
.
126
7.3.3
CULTIVATION
OF
THE
HYBRIDOMA
CELL
LINE
IV
F19.23
.
129
7.4
DISCUSSION
.
130
7.5
CONSIDERATIONS
FOR
THE
INDUSTRIAL
SCALE
.
134
7.5.1
DESIGN
.
136
7.5.2
HYDRODYNAMIC
FLOW
IN
LARGE-SCALE
SYSTEMS
.
137
7.5.3
CULTIVATION
PARAMETER
.
138
8
SUMMARY
.
138
9
APPENDIX
.
141
9.1
MATLAB
ROUTINE
FOR
RTD
EXPERIMENTS
.
141
9.1.1
MAIN
PROGRAM
.141
-*
9.1.2
SUBROUTINE
TO
CONSTRUCT
THE
VECTOR
-
C
.
142
AT
9.2
MEDIA
FORMULATION
.
143
10
REFERENCES
.
145 |
| any_adam_object | 1 |
| author | Fassnacht, Dieter |
| author_facet | Fassnacht, Dieter |
| author_role | aut |
| author_sort | Fassnacht, Dieter |
| author_variant | d f df |
| building | Verbundindex |
| bvnumber | BV014151523 |
| classification_tum | CIT 310d CIT 380d |
| ctrlnum | (OCoLC)49636416 (DE-599)BVBBV014151523 |
| discipline | Chemie-Ingenieurwesen |
| edition | Als Ms. gedr. |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV014151523 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:31:03Z |
| institution | BVB |
| isbn | 3183209179 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009698804 |
| oclc_num | 49636416 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-83 |
| owner_facet | DE-91G DE-BY-TUM DE-83 |
| physical | XIV, 157 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | VDI-Verl. |
| record_format | marc |
| series | Fortschrittberichte VDI |
| series2 | Fortschrittberichte VDI : Reihe 17, Biotechnik |
| spelling | Fassnacht, Dieter Verfasser aut Fixed-bed reactors for the cultivation of animal cells Dieter Fassnacht Als Ms. gedr. Düsseldorf VDI-Verl. 2001 XIV, 157 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Fortschrittberichte VDI : Reihe 17, Biotechnik 209 Zugl.: Hamburg-Harburg, Techn. Univ., Diss., 2000 Bioreactors Biotechnology instrumentation Biotechnology methods Cell Culture Techniques methods Genetic Engineering methods Zellkultur (DE-588)4067547-6 gnd rswk-swf Hybridoma-Zelle (DE-588)4382182-0 gnd rswk-swf Apoptosis (DE-588)4291096-1 gnd rswk-swf Reaktionstechnik (DE-588)4136173-8 gnd rswk-swf Maßstabübertragung (DE-588)4279219-8 gnd rswk-swf Festbettreaktor (DE-588)4113549-0 gnd rswk-swf Tierzelle (DE-588)4185509-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tierzelle (DE-588)4185509-7 s Zellkultur (DE-588)4067547-6 s Reaktionstechnik (DE-588)4136173-8 s Festbettreaktor (DE-588)4113549-0 s DE-604 Maßstabübertragung (DE-588)4279219-8 s Hybridoma-Zelle (DE-588)4382182-0 s Apoptosis (DE-588)4291096-1 s Fortschrittberichte VDI Reihe 17, Biotechnik ; 209 (DE-604)BV000903783 209 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009698804&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fassnacht, Dieter Fixed-bed reactors for the cultivation of animal cells Fortschrittberichte VDI Bioreactors Biotechnology instrumentation Biotechnology methods Cell Culture Techniques methods Genetic Engineering methods Zellkultur (DE-588)4067547-6 gnd Hybridoma-Zelle (DE-588)4382182-0 gnd Apoptosis (DE-588)4291096-1 gnd Reaktionstechnik (DE-588)4136173-8 gnd Maßstabübertragung (DE-588)4279219-8 gnd Festbettreaktor (DE-588)4113549-0 gnd Tierzelle (DE-588)4185509-7 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4382182-0 (DE-588)4291096-1 (DE-588)4136173-8 (DE-588)4279219-8 (DE-588)4113549-0 (DE-588)4185509-7 (DE-588)4113937-9 |
| title | Fixed-bed reactors for the cultivation of animal cells |
| title_auth | Fixed-bed reactors for the cultivation of animal cells |
| title_exact_search | Fixed-bed reactors for the cultivation of animal cells |
| title_full | Fixed-bed reactors for the cultivation of animal cells Dieter Fassnacht |
| title_fullStr | Fixed-bed reactors for the cultivation of animal cells Dieter Fassnacht |
| title_full_unstemmed | Fixed-bed reactors for the cultivation of animal cells Dieter Fassnacht |
| title_short | Fixed-bed reactors for the cultivation of animal cells |
| title_sort | fixed bed reactors for the cultivation of animal cells |
| topic | Bioreactors Biotechnology instrumentation Biotechnology methods Cell Culture Techniques methods Genetic Engineering methods Zellkultur (DE-588)4067547-6 gnd Hybridoma-Zelle (DE-588)4382182-0 gnd Apoptosis (DE-588)4291096-1 gnd Reaktionstechnik (DE-588)4136173-8 gnd Maßstabübertragung (DE-588)4279219-8 gnd Festbettreaktor (DE-588)4113549-0 gnd Tierzelle (DE-588)4185509-7 gnd |
| topic_facet | Bioreactors Biotechnology instrumentation Biotechnology methods Cell Culture Techniques methods Genetic Engineering methods Zellkultur Hybridoma-Zelle Apoptosis Reaktionstechnik Maßstabübertragung Festbettreaktor Tierzelle Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009698804&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000903783 |
| work_keys_str_mv | AT fassnachtdieter fixedbedreactorsforthecultivationofanimalcells |