Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English German |
| Veröffentlicht: |
2001
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2002 |
| Beschreibung: | 118 Bl. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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CONTENTS
SYNOPSIS.5
ZUSAMMENFASSUNG.6
1. INTRODUCTION.8
CRYPTOSPORIDIUM PARVUM
.8
DISEASES CAUSED BY C.
PARVUM AND ROUTE OF TRANSMISSION.8
LIFE CYCLE OF C.
PARVUM.8
CHALLENGES ENCOUNTERED IN ATTEMPTS TO REMOVE C.
PARVUM FROM WATER.9
DETECTING
C. PARVUM USING ANTIBODIES.10
DETECTING
C. PARVUM USING SYNTHETIC NUCLEIC ACIDS.11
IMPORTANCE OF NUCLEIC ACID DIAGNOSTICS FOR THE DETECTION OF PATHOGENS.12
TRADITIONAL NUCLEIC ACID DETECTION TECHNIQUES.13
NUCLEIC ACID DETECTION BY OLIGONUCLEOTIDE-TAGGED LIPOSOMES.14
2. DEVELOPMENT OF A TEST-STRIP FOR THE RAPID AND SENSITIVE DETECTION OF
CRYPTOSPORIDIUM PARVUM.16
ABSTRACT.16
INTRODUCTION.17
BACKGROUND.17
DETECTION OF C.
PARVUM BY USING A NOVEL TEST-STRIP.18
EXPERIMENTAL SECTION.19
REAGENTS.19
OLIGOMERS.20
ENCAPSULANT PREPARATION.20
LIPOSOME PREPARATION.21
CONJUGATING REPORTER PROBES TO LIPOSOMES.21
PREPARATION OF TEST-STRIPS.22
ASSAY PROTOCOL.24
DETECTION AND QUANTIFICATION. 24
NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (NASBA).24
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/964221683
RESULTS AND DISCUSSION.26
CHARACTERIZATION OF LIPOSOMES.26
OPTIMIZING THE TEST-STRIP.26
DETERMINING THE LIMIT OF DETECTION.30
DETERMINING THE COMPATIBILITY OF THE TEST-STRIP ASSAY
WITH THE NASBA METHODOLOGY.33
SPECIFICITY OF THE TEST.35
CONCLUSIONS.26
3. DEVELOPMENT OF A MICROFLUIDIC CHIP FOR THE DETECTION OF
CRYPTOSPORIDIUM
PARVUM BY MEANS OF FLUORESCENT SIGNALS GENERATED BY LIPOSOMES.37
ABSTRACT.27
INTRODUCTION.28
BACKGROUND.38
THE CONCEPT OF THE MICROFLUIDIC CHIP.40
EXPERIMENTAL SECTION.-41
OLIGOMERS.41
PREPARATION OF ACETYLTHIOACETATE (ATA)-TAGGED LIPOSOMES CONTAINING
CARBOXYFLUORESCEIN.41
CONJUGATING REPORTER PROBES TO ATA-TAGGED LIPOSOMES.42
PREPARATION OF CAPTURE PROBE/HEXANE MONOLAYERS ON GOLD-COVERED
GLASS SLIDES.-44
PREPARATION OF THE MICROFLUIDIC CHIP.44
ASSAY PROTOCOL.-^5
DETECTION AND QUANTIFICATION.46
NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (NASBA).46
RESULTS AND DISCUSSION.-47
DESIGN AND FABRICATION OF THE MICROFLUIDIC CHIP.47
MINIMIZING THE BACKGROUND SIGNALS GENERATED IN THE CHIP.50
DETERMINING THE LIMIT OF DETECTION.53
SPECIFICITY OF THE ASSAY.
56
TESTING OF RNA AMPLIFIED BY NASBA.57
REUSABILITY OF THE CHIP.58
59
CONCLUSIONS.
2
4. DEVELOPMENT OF A PROTOTYPE MICROFLUIDIC ARRAY FOR
MULTIPLE ANALYTE DETECTION.60
ABSTRACT.60
INTRODUCTION.60
BACKGROUND.60
FABRICATION OF A PROTOTYPE MICROFLUIDIC ARRAY.63
EXPERIMENTAL SECTION.64
OLIGOMERS.64
PREPARATION OF ACETYLTHIOACETATE (ATA)-TAGGED LIPOSOMES CONTAINING
CARBOXYFLUORESCEIN.64
PREPARATION OF GOLD-COVERED MICROSCOPE-GLASS SLIDES.64
PREPARATION OF THE MICROFLUIDIC ARRAY.64
ASSAY PROTOCOL.66
DETECTION AND QUANTIFICATION.66
RESULTS AND DISCUSSION.67
DESIGN AND FABRICATION OF THE MICROFLUIDIC ARRAY.67
MINIMIZING THE BACKGROUND BINDING.68
ANALYZING MULTIPLE SAMPLES SIMULTANEOUSLY.71
CONCLUSIONS.72
5. ELECTROCHEMICAL DETECTION OF
CRYPTOSPORIDIUM PARVUM USING INTERDIGITATED
MICROELECTRODE ARRAYS IN A MICROFLUIDIC 73
CHIP.
ABSTRACT.73
INTRODUCTION.73
BACKGROUND.73
ELECTROCHEMICAL DETECTION USING LIPOSOMES AND
INTERDIGITATED MICROELECTRODE ARRAYS.75
EXPERIMENTAL SECTION.76
OLIGOMERS.76
PREPARATION OF REPORTER PROBE-TAGGED LIPOSOMES CONTAINING FERRI- AND
FERROHEXACYANIDE.76
PREPARATION OF INTERDIGITATED ELECTRODE ARRAYS.76
PREPARATION OF THE MICROFLUIDIC CHIP.78
3
ASSAY PROCEDURE.78
ELECTROCHEMICAL DETECTION AND QUANTIFICATION.79
RESULTS AND DISCUSSION.80
DESIGN AND FABRICATION OF THE CHIP.80
CHARACTERIZATION OF LIPOSOMES.83
DETECTING FERRI- AND FERROHEXACYANIDE USING INTERDIGITATED ELECTRODE
ARRAYS.83
INFLUENCE OF ASSAY REAGENTS ON THE DETECTION
WITH INTERDIGITATED ELECTRODES.86
DOSE-RESPONSE CURVE.90
CONCLUSIONS.91
6. OLIGONUCLEOTIDE DETECTION BY SURFACE PLASMON RESONANCE
SPECTROSCOPY.92
ABSTRACT.92
INTRODUCTION.92
BACKGROUND.92
SPR AND NUCLEIC ACID HYBRIDIZATION.94
EXPERIMENTAL SECTION.95
OLIGOMERS.95
PREPARATION OF REPORTER PROBE-TAGGED LIPOSOMES.95
SPR EXPERIMENTAL SETUP.95
DEPOSITING GOLD ON THE LENS.96
PREPARING ORGANIC LAYERS ON THE GOLD SURFACE.96
ASSAY PROTOCOL.97
DATA ANALYSIS.98
RESULTS AND DISCUSSION.99
IMMOBILIZATION OF CAPTURE PROBES AND DEPOSITION OF BLOCKING
LAYERS ON A GOLD SURFACE.99
HYBRIDIZATION OF TARGETS TO MIXED LAYERS OF CAPTURE PROBES
AND BLOCKING MOLECULES.102
SANDWICH-HYBRIDIZATION WITH REPORTER PROBE-TAGGED LIPOSOMES.103
CONCLUSIONS.105
7. REFERENCES OF CHAPTERS 1 TO 6.107
4 |
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| physical | 118 Bl. Ill., graph. Darst. |
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| spelling | Esch, Mandy Verfasser aut Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum von Mandy Esch 2001 118 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2002 Cryptosporidium (DE-588)4224195-9 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Cryptosporidium (DE-588)4224195-9 s RNS (DE-588)4076759-0 s Biosensor (DE-588)4193016-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009696871&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Esch, Mandy Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum Cryptosporidium (DE-588)4224195-9 gnd Biosensor (DE-588)4193016-2 gnd RNS (DE-588)4076759-0 gnd |
| subject_GND | (DE-588)4224195-9 (DE-588)4193016-2 (DE-588)4076759-0 (DE-588)4113937-9 |
| title | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum |
| title_auth | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum |
| title_exact_search | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum |
| title_full | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum von Mandy Esch |
| title_fullStr | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum von Mandy Esch |
| title_full_unstemmed | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum von Mandy Esch |
| title_short | Novel nucleic acid sensors for the rapid detection of Cryptosporidium parvum |
| title_sort | novel nucleic acid sensors for the rapid detection of cryptosporidium parvum |
| topic | Cryptosporidium (DE-588)4224195-9 gnd Biosensor (DE-588)4193016-2 gnd RNS (DE-588)4076759-0 gnd |
| topic_facet | Cryptosporidium Biosensor RNS Hochschulschrift |
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