Directed molecular evolution of proteins or how to improve enzymes for biocatalysis:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 357 S. Ill., graph. Darst. |
| ISBN: | 3527304231 |
Internformat
MARC
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| 245 | 1 | 0 | |a Directed molecular evolution of proteins or how to improve enzymes for biocatalysis |c ed. by Susanne Brakmann ... |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2002 | |
| 300 | |a XI, 357 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Bioquímica |2 larpcal | |
| 650 | 4 | |a Chimie combinatoire | |
| 650 | 4 | |a Enzymes - Biotechnologie | |
| 650 | 7 | |a Evolução molecular |2 larpcal | |
| 650 | 7 | |a Proteínas |2 larpcal | |
| 650 | 4 | |a Protéines - Modification chimique | |
| 650 | 4 | |a Protéines - Évolution | |
| 650 | 7 | |a Química orgânica |2 larpcal | |
| 650 | 4 | |a Évolution moléculaire | |
| 650 | 4 | |a Directed Molecular Evolution | |
| 650 | 4 | |a Molecular evolution | |
| 650 | 4 | |a Protein Engineering | |
| 650 | 4 | |a Proteins |x Chemical modification | |
| 650 | 4 | |a Proteins |x Evolution | |
| 650 | 0 | 7 | |a Mutagenese |0 (DE-588)4170881-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzymkatalyse |0 (DE-588)4152480-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzym |0 (DE-588)4014988-2 |2 gnd |9 rswk-swf |
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| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Brakmann, Susanne |d 1964- |e Sonstige |0 (DE-588)11284717X |4 oth | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009689891&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009689891 | |
Datensatz im Suchindex
| _version_ | 1816444698863075328 |
|---|---|
| adam_text |
V
CONTENTS
LIST
OF
CONTRIBUTORS
XI
1
INTRODUCTION
1
2
EVOLUTIONARY
BIOTECHNOLOGY
-
FROM
IDEAS
AND
CONCEPTS
TO
EXPERIMENTS
AND
COMPUTER
SIMULATIONS
5
2.1
EVOLUTION
IN
VIVO
-
FROM
NATURAL
SELECTION
TO
POPULATION
GENETICS
5
2.2
EVOLUTION
IN
VITRO
-
FROM
KINETIC
EQUATIONS
TO
MAGIC
MOLECULES
8
2.3
EVOLUTION
IN
SILICO
-
FROM
NEUTRAL
NETWORKS
TO
MULTI-STABLE
MOLECULES
16
2.4
SEQUENCE
STRUCTURE
MAPPINGS
OF
PROTEINS
25
2.5
CONCLUDING
REMARKS
26
3
USING
EVOLUTIONARY
STRATEGIES
TO
INVESTIGATE
THE
STRUCTURE
AND
FUNCTION
OF
CHORISMATE
MUTASES
29
3.1
INTRODUCTION
29
3.2
SELECTION
VERSUS
SCREENING
30
3.2.1
CLASSICAL
SOLUTIONS
TO
THE
SORTING
PROBLEM
31
3.2.2
ADVANTAGES
AND
LIMITATIONS
OF
SELECTION
32
3.3
GENETIC
SELECTION
OF
NOVEL
CHORISMATE
MUTASES
33
3.3.1
THE
SELECTION
SYSTEM
35
3.3.2
MECHANISTIC
STUDIES
37
3.3.2.1
ACTIVE
SITE
RESIDUES
37
3.3.2.2
RANDOM
PROTEIN
TRUNCATION
42
3.3.3
STRUCTURAL
STUDIES
44
3.3.3.1
CONSTRAINTS
ON
INTERHELICAL
LOOPS
44
3.3.4
ALTERING
PROTEIN
TOPOLOGY
46
3.3.4.1
NEW
QUATERNARY
STRUCTURES
47
3.3.4.2
STABLE
MONOMERIC
MUTASES
49
3.3.5
AUGMENTING
WEAK
ENZYME
ACTIVITY
51
3.3.6
PROTEIN
DESIGN
53
3.4
SUMMARY
AND
GENERAL
PERSPECTIVES
57
VI
CONTENTS
4
CONSTRUCTION
OF
ENVIRONMENTAL
LIBRARIES
FOR
FUNCTIONAL
SCREENING
OF
ENZYME
ACTIVITY
63
4.1
4.2
4.3
4.4
SAMPLE
COLLECTION
AND
DNA
ISOLATION
FROM
ENVIRONMENTAL
SAMPLES
65
CONSTRUCTION
OF
ENVIRONMENTAL
LIBRARIES
68
SCREENING
OF
ENVIRONMENTAL
LIBRARIES
71
CONCLUSIONS
76
5
5.1
5.2
5.3
5.3.1
5.3.1.1
5.3.1.2
5.3.2
5.4
5.5
5.5.1
5.5.2
5.5.2.1
5.5.2.2
5.5.2.3
5.5.2.4
5.5.2.S
5.6
INVESTIGATION
OF
PHAGE
DISPLAY
FOR
THE
DIRECTED
EVOLUTION
OF
ENZYMES
79
INTRODUCTION
79
THE
PHAGE
DISPLAY
79
PHAGE
DISPLAY
OF
ENZYMES
81
THE
EXPRESSION
VECTORS
81
FILAMENTOUS
BACTERIOPHAGES
81
OTHER
PHAGES
83
PHAGE-ENZYMES
84
CREATING
LIBRARIES
OF
MUTANTS
87
SELECTION
OF
PHAGE-ENZYMES
89
SELECTION
FOR
BINDING
89
SELECTION
FOR
CATALYTIC
ACTIVITY
90
SELECTION
WITH
SUBSTRATE
OR
PRODUCT
ANALOGUES
90
SELECTION
WITH
TRANSITION-STATE
ANALOGUES
92
SELECTION
OF
REACTIVE
ACTIVE
SITE
RESIDUES
BY
AFFINITY
LABELING
96
SELECTION
WITH
SUICIDE
SUBSTRATES
98
SELECTIONS
BASED
DIRECTLY
ON
SUBSTRATE
TRANSFORMATIONS
102
CONCLUSIONS
108
6
DIRECTED
EVOLUTION
OF
BINDING
PROTEINS
BY
CELL
SURFACE
DISPLAY:
ANALYSIS
OF
THE
SCREENING
PROCESS
111
6.1
6.2
6.2.1
6.2.2
6.3
6.3.1
6.3.2
6.4
INTRODUCTION
111
LIBRARY
CONSTRUCTION
113
MUTAGENESIS
113
EXPRESSION
114
MUTANT
ISOLATION
115
DIFFERENTIAL
LABELING
115
SCREENING
119
SUMMARY
124
ACKNOWLEDGMENTS
124
8
8.1
8.2
8.3
8.4
ADVANCED
SCREENING
STRATEGIES
FOR
BIOCATALYST
DISCOVERY
159
INTRODUCTION
159
SEMI-QUANTITATIVE
SCREENING
IN
AGAR-PLATE
FORMATS
161
SOLUTION-BASED
SCREENING
IN
MICROPLATE
FORMATS
164
ROBOTICS
AND
AUTOMATION
169
9
9.1
ENGINEERING
PROTEIN
EVOLUTION
177
INTRODUCTION
177
VII
9.2
9.2.1
9.2.2
MECHANISMS
OF
PROTEIN
EVOLUTION
IN
NATURE
178
GENE
DUPLICATION
179
TANDEM
DUPLICATION
180
PA-BARRELS
181
9.2.3
9.2.4
9.2.5
9.2.6
9.2.7
9.3
9.3.1
9.3.2
9.3.3
9.3.4
9.3.5
9.3.6
9.4
9.4.1
9.4.2
9.4.3
9.5
CIRCULAR
PERMUTATION
182
OLIGOMERIZATION
183
GENE
FUSION
184
DOMAIN
RECRUITMENT
184
EXON
SHUFFLING
186
ENGINEERING
GENES
AND
GENE
FRAGMENTS
187
PROTEIN
FRAGMENTATION
188
RATIONAL
SWAPPING
OF
SECONDARY
STRUCTURE
ELEMENTS
AND
DOMAINS
189
COMBINATORIAL
GENE
FRAGMENT
SHUFFLING
190
MODULAR
RECOMBINATION
AND
PROTEIN
FOLDING
194
RATIONAL
DOMAIN
ASSEMBLY
-
ENGINEERING
ZINC
FINGERS
199
COMBINATORIAL
DOMAIN
RECOMBINATION
-
EXON
SHUFFLING
200
GENE
FUSION
-
FROM
BI
TO
MULTIFUNCTIONAL
ENZYMES
203
END-TO-END
GENE
FUSIONS
203
GENE
INSERTIONS
203
MODULAR
DESIGN
IN
MULTIFUNCTIONAL
ENZYMES
204
PERSPECTIVES
208
10
10.1
10.2
10.3
10.3.1
10.3.1
10.3.2.1
10.3.2.2
10.3.2.3
10.4
10.4.1
10.4.2
10.4.2.1
10.4.2.2
10.4.2.3
10.4.2.4
10.4.2.5
10.5
10.6
10.7
10.7.1
10.7.1.1
10.7.1.2
EXPLORING
THE
DIVERSITY
OF
HEME
ENZYMES
THROUGH
DIRECTED
EVOLUTION
215
INTRODUCTION
215
HEME
PROTEINS
216
CYTOCHROMES
P450
218
INTRODUCTION
218
MECHANISM
220
THE
CATALYTIC
CYCLE
220
UNCOUPLING
222
PEROXIDE
SHUNT
PATHWAY
222
PEROXIDASES
223
INTRODUCTION
223
MECHANISM
223
COMPOUND
I
FORMATION
223
OXIDATIVE
DEHYDROGENATION
226
OXIDATIVE
HALOGENATION
226
PEROXIDE
DISPROPORTIONATION
226
OXYGEN
TRANSFER
227
COMPARISON
OF
P450S
AND
PEROXIDASES
227
CHLOROPEROXIDASE
228
MUTAGENESIS
STUDIES
229
P450S
230
P450
CAM
230
EUKARYOTIC
P450S
230
VIII
CONTENTS
10.7.2
10.7.3
10.7.4
10.8
10.8.1
10.8.2
10.8.3
10.8.4
10.8.5
10.8.6
10.9
HRP
231
CPO
231
MYOGLOBIN
(MB)
232
DIRECTED
EVOLUTION
OF
HEME
ENZYMES
233
P450S
233
PEROXIDASES
234
CPO
236
CATALASE
I
236
MYOGLOBIN
237
METHODS
FOR
RECOMBINATION
OF
P450S
237
CONCLUSIONS
238
11
DIRECTED
EVOLUTION
AS
A
MEANS
TO
CREATE
ENANTIOSELECTIVE
ENZYMES
FOR
USE
IN
ORGANIC
CHEMISTRY
245
11.1
11.2
11.3
11.4
11.5
11.5.1
11.5.2
INTRODUCTION
245
MUTAGENESIS
METHODS
247
OVEREXPRESSION
OF
GENES
AND
SECRETION
OF
ENZYMES
248
HIGH-THROUGHPUT
SCREENING
SYSTEMS
FOR
ENANTIOSELECTIVITY
250
EXAMPLES
OF
DIRECTED
EVOLUTION
OF
ENANTIOSELECTIVE
ENZYMES
257
KINETIC
RESOLUTION
OF
A
CHIRAL
ESTER
CATALYZED
BY
MUTANT
LIPASES
257
EVOLUTION
OF
A
LIPASE
FOR
THE
STEREOSELECTIVE
HYDROLYSIS
OF
A
MESO-COMPOUND
268
11.5.3
11.5.4
11.5.5
11.5.6
11.6
KINETIC
RESOLUTION
OF
A
CHIRAL
ESTER
CATALYZED
BY
A
MUTANT
ESTERASE
269
IMPROVING
THE
ENANTIOSELECTIVITY
OF
A
TRANSAMINASE
270
INVERSION
OF
THE
ENANTIOSELECTIVITY
OF
A
HYDANTOINASE
270
EVOLVING
ALDOLASES
WHICH
ACCEPT
BOTH
D
-
AND
L-GLYCERALDEHYDES
271
CONCLUSIONS
273
12
APPLIED
MOLECULAR
EVOLUTION
OF
ENZYMES
INVOLVED
IN
SYNTHESIS
AND
REPAIR
OF
DNA
281
12.1
12.2
12.2.1
12.2.2
12.2.3
12.2.4
12.2.5
12.2.6
INTRODUCTION
281
DIRECTED
EVOLUTION
OF
ENZYMES
282
SITE-DIRECTED
MUTAGENESIS
283
DIRECTED
EVOLUTION
284
GENETIC
DAMAGE
285
PCR
MUTAGENESIS
286
DNA
SHUFFLING
287
SUBSTITUTION
BY
OLIGONUCLEOTIDES
CONTAINING
RANDOM
MUTATIONS
(RANDOM
MUTAGENESIS)
288
12.3
12.3.1
12.3.1.1
DIRECTED
EVOLUTION
OF
DNA
POLYMERASES
289
RANDOM
MUTAGENESIS
OF
THERMUS
AQUATICUS
DNA
POL
I
291
DETERMINATION
OF
STRUCTURAL
COMPONENTS
FOR
TAQ
DNA
POLYMERASE
FIDELITY
292
12.3.1.2
12.3.1.3
DIRECTED
EVOLUTION
OF
A
RNA
POLYMERASE
FROM
TAQ
DNA
POLYMERASE
293
MUTABILITY
OF
THE
TAQ
POLYMERASE
ACTIVE
SITE
294
IX
INDEX
343
12.3.2
12.4
12.5
12.6
12.7
RANDOM
OLIGONUCLEOTIDE
MUTAGENESIS
OF
ESCHERICHIA
COLI
POL
I
294
DIRECTED
EVOLUTION
OF
THYMIDINE
KINASE
295
DIRECTED
EVOLUTION
OF
THYMIDYLATE
SYNTHASE
297
O
6
-ALKYLGUANINE-DNA
ALKYLTRANSFERASE
300
DISCUSSION
302
13
EVOLUTIONARY
GENERATION
VERSUS
RATIONAL
DESIGN
OF
RESTRICTION
ENDONUCLEASES
WITH
NOVEL
SPECIFICITY
309
13.1
13.1.1
13.1.2
13.1.3
13.1.4
INTRODUCTION
309
BIOLOGY
OF
RESTRICTION/MODIFICATION
SYSTEMS
309
BIOCHEMICAL
PROPERTIES
OF
TYPE
II
RESTRICTION
ENDONUCLEASES
310
APPLICATIONS
FOR
TYPE
II
RESTRICTION
ENDONUCLEASES
311
SETTING
THE
STAGE
FOR
PROTEIN
ENGINEERING
OF
TYPE
II
RESTRICTION
ENDONUCLEASES
313
13.2
13.2.1
13.2.1.1
DESIGN
OF
RESTRICTION
ENDONUCLEASES
WITH
NEW
SPECIFICITIES
313
RATIONAL
DESIGN
313
ATTEMPTS
TO
EMPLOY
RATIONAL
DESIGN
TO
CHANGE
THE
SPECIFICITY
OF
RESTRICTION
ENZYMES
313
14.2.1.1
CHANGING
THE
SUBSTRATE
SPECIFICITY
OF
TYPE
ILS
RESTRICTION
ENZYMES
BY
DOMAIN
FUSION
316
13.2.1.3
13.2.2
13.3
RATIONAL
DESIGN
TO
EXTEND
SPECIFICITIES
OF
TYPE
II
RESTRICTION
ENZYMES
316
EVOLUTIONARY
DESIGN
OF
EXTENDED
SPECIFICITIES
318
SUMMARY
AND
OUTLOOK
324
14
14.1
14.2
14.3
14.3.1
14.3.2
14.3.3
14.4
EVOLUTIONARY
GENERATION
OF
ENZYMES
WITH
NOVEL
SUBSTRATE
SPECIFICITIES
329
INTRODUCTION
329
GENERAL
CONSIDERATIONS
331
EXAMPLES
333
GROUP
1
333
GROUP
2
337
GROUP
3
338
CONCLUSIONS
339 |
| any_adam_object | 1 |
| author_GND | (DE-588)11284717X |
| building | Verbundindex |
| bvnumber | BV014140636 |
| callnumber-first | Q - Science |
| callnumber-label | QP551 |
| callnumber-raw | QP551 TP248.65.E59 |
| callnumber-search | QP551 TP248.65.E59 |
| callnumber-sort | QP 3551 |
| callnumber-subject | QP - Physiology |
| classification_rvk | VK 8700 WD 5050 |
| classification_tum | CHE 825f |
| ctrlnum | (OCoLC)48153128 (DE-599)BVBBV014140636 |
| dewey-full | 547.750442 660.6/34 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 547 - Organic chemistry 660 - Chemical engineering |
| dewey-raw | 547.750442 660.6/34 |
| dewey-search | 547.750442 660.6/34 |
| dewey-sort | 3547.750442 |
| dewey-tens | 540 - Chemistry and allied sciences 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV014140636 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:30:53Z |
| institution | BVB |
| isbn | 3527304231 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009689891 |
| oclc_num | 48153128 |
| open_access_boolean | |
| owner | DE-703 DE-355 DE-BY-UBR DE-12 DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-526 DE-91G DE-BY-TUM |
| owner_facet | DE-703 DE-355 DE-BY-UBR DE-12 DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-526 DE-91G DE-BY-TUM |
| physical | XI, 357 S. Ill., graph. Darst. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis ed. by Susanne Brakmann ... Weinheim Wiley-VCH 2002 XI, 357 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bioquímica larpcal Chimie combinatoire Enzymes - Biotechnologie Evolução molecular larpcal Proteínas larpcal Protéines - Modification chimique Protéines - Évolution Química orgânica larpcal Évolution moléculaire Directed Molecular Evolution Molecular evolution Protein Engineering Proteins Chemical modification Proteins Evolution Mutagenese (DE-588)4170881-7 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Enzym (DE-588)4014988-2 s Mutagenese (DE-588)4170881-7 s Enzymkatalyse (DE-588)4152480-9 s DE-604 Brakmann, Susanne 1964- Sonstige (DE-588)11284717X oth DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009689891&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis Bioquímica larpcal Chimie combinatoire Enzymes - Biotechnologie Evolução molecular larpcal Proteínas larpcal Protéines - Modification chimique Protéines - Évolution Química orgânica larpcal Évolution moléculaire Directed Molecular Evolution Molecular evolution Protein Engineering Proteins Chemical modification Proteins Evolution Mutagenese (DE-588)4170881-7 gnd Enzymkatalyse (DE-588)4152480-9 gnd Enzym (DE-588)4014988-2 gnd |
| subject_GND | (DE-588)4170881-7 (DE-588)4152480-9 (DE-588)4014988-2 (DE-588)4143413-4 |
| title | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis |
| title_auth | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis |
| title_exact_search | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis |
| title_full | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis ed. by Susanne Brakmann ... |
| title_fullStr | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis ed. by Susanne Brakmann ... |
| title_full_unstemmed | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis ed. by Susanne Brakmann ... |
| title_short | Directed molecular evolution of proteins or how to improve enzymes for biocatalysis |
| title_sort | directed molecular evolution of proteins or how to improve enzymes for biocatalysis |
| topic | Bioquímica larpcal Chimie combinatoire Enzymes - Biotechnologie Evolução molecular larpcal Proteínas larpcal Protéines - Modification chimique Protéines - Évolution Química orgânica larpcal Évolution moléculaire Directed Molecular Evolution Molecular evolution Protein Engineering Proteins Chemical modification Proteins Evolution Mutagenese (DE-588)4170881-7 gnd Enzymkatalyse (DE-588)4152480-9 gnd Enzym (DE-588)4014988-2 gnd |
| topic_facet | Bioquímica Chimie combinatoire Enzymes - Biotechnologie Evolução molecular Proteínas Protéines - Modification chimique Protéines - Évolution Química orgânica Évolution moléculaire Directed Molecular Evolution Molecular evolution Protein Engineering Proteins Chemical modification Proteins Evolution Mutagenese Enzymkatalyse Enzym Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009689891&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT brakmannsusanne directedmolecularevolutionofproteinsorhowtoimproveenzymesforbiocatalysis |