Arabidopsis: a laboratory manual
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cold Spring Harbor, NY
Cold Spring Harbor Laboratory Pr.
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 354 S. in Ringbuch Ill. |
| ISBN: | 0879695722 0879695730 |
Internformat
MARC
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| 650 | 4 | |a Arabidopsis |x cytology |v Laboratory Manuals | |
| 650 | 4 | |a Arabidopsis |x genetics |v Laboratory Manuals | |
| 650 | 4 | |a Mutation |v Laboratory Manuals | |
| 650 | 4 | |a Plants, Genetically Modified |x genetics |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804128977231544320 |
|---|---|
| adam_text | Contents
Preface,
Chapter
How to Grow Arabidopsis,
Morphology and Anatomy
Seed Collection and Storage
Cultivation of Arabidopsis
General Considerations
Growth Environment
Cultivation of Plants in Soil
Use of Solid Media
Other Growth Conditions
Pest Management
Supplier s Web Sites
References
Chapter
Obtaining Mutants,
Obtaining Previously Identified Mutants from the Stock Centers
Forward Genetics: Finding Mutations That Cause Particular
Phenotypes
EMS Mutagenesis of Seed
Т
Special
Reverse Genetics: Finding Mutations in Particular Genes
Screening
Considerations for Phenotypic Characterization
Beyond Mutants: Using Natural Variation to Identify Interesting
Genes
References
V¡
Chapter
Genetic Analysis of Mutants,
Setting up Crosses
Choosing Parent Plants
Choosing Flowers
Segregation Analysis
Self-progeny
Fj Progeny
F2 Progeny
Backcrosses and Cosegregation
Complementation Testing
Strategies for Identifying Double Mutants
Finding Double Mutants in the F2 Generation
Finding Double Mutants in the F3 Generation
The Brute Force Approach
References
Chapter
How to Analyze a Mutant Phenotypically,
Growth Parameters
Quantitative Analysis of Root Growth
Hypocotyl Length
Flowering Time
Germination Rate
Fresh Weight Gain
Water Loss
Hormone Response
Gibberellin/Abscisic Acid/Paclobutrazol
Auxin
Ethylene
Brassinosteroids
Response to the Abiotic Environment
Root Elongation Under Salt/Hormone-induced Stress
Germination Rate
Electrolyte Leakage
Fresh Weight Gain
Water Loss
Others:
Heavy Metal Stress
Bacterial Pathogens
Preparation of Bacterial Cultures and Inoculation of Plants
Testing the Hypersensitive Response
Assessing Bacterial Growth
Oomycete Pathogens:
Reviving Frozen Stocks and Inoculating Plants
Rating
CONTENTS ·
Preparation of Frozen Tissue Sections
Maintaining Infections
Diaminobenzidine Stain for Hydrogen Peroxide
Trypan Blue Stain for Fungi, Oomycetes, and Dead Plant Cells
Histology
Preparation of Tissue Sections of Fixed Material
Specialized Staining Techniques
The PAS Reaction for Staining Cell Walls
Alcian Blue-PAS Reaction
Phloroglucinol Stain for
Vital Stain for Cytoplasm
Neutral Red Staining for
Whole-mount DAPI Staining and Measurement of
DNA
Nuclear Staining for Confocal Microscopy
Cleared Tissue for Observation of Vascular Strands
Agarose
Scanning Electron Microscopy
Standard
Dental Wax Impressions to be Viewed in
SEM Quick-and-Easy
Imaging of Fresh Arabidopsis Tissues in the
Transmission Electron Microscopy
Standard
ТЕМ
References
Chapter
How to Transform Arabidopsis,
Vectors and Agrobacterium Hosts
Agrobacterium strains
Transformation of Agrobacterium
Transformation of Agrobacterium Using Electroporation
Transformation of Agrobacterium Using the Freeze-Thaw
Method
PCR Analysis of Agrobacterium
In
Plant Growth
Floral Dip of Arabidopsis
Alternative Protocol
Alternative Protocol
Selecting Transformed Plants
Kanamycin Selection
Basta
Root Transformation
References
VIU
Chapter
How to Isolate a Gene Defined by a Mutation,
Isolating a Gene Defined by an Insertion Mutation
TAIL-PCR
Isolating a Gene Known Only by the Phenotypes of Mutant
Alíeles:
STEP
STEP
STEP
Special Cases
Serious Errors
Purifying
СТАВ
Dellaporta
Quick
References
Chapter
How to Study Gene Expression,
RNA
RNA
Semiquantitative
In Situ Hybridization to Tissue Sections
Radioactive In Situ Hybridization
Nonradioactive In Situ Hybridization
Whole-mount In Situ Hybridization
Protein Expression
Extraction of Total Protein for Western Blots
Organelle
FPLC Gel Filtration
Nondenaturing Gel Electrophoresis of Proteins
Protein Coimmunoprecipitation
In Situ Localization of Proteins
Reporter Genes
Whole-mount
Quantitative
Subcellular Localization of
Onion Epidermal Cells
Live-cell Imaging of GFP
Liquid LUC Activity Assays
LUC Imaging of Whole Plants
References
CONTENTS
Chapter
How to Study Gene Function,
Reducing Gene Expression
Antisense
Cosuppression/RNAi
Tissue- or Stage-specific Gene Knock-outs
Misexpression
Constitutive/Tissue-specific Promoter Fusions
Two-component Systems for Tissue-specific Misexpression
The Ale Gene Ethanol-inducible Switch system
Glucocorticoid Inducible Control of Gene Expression
Heat Shock Induction
Glucocorticoid Fusions for Transcription Factors
Transient Expression
Transient Expression in Protoplasts
Transgene
Other Gain-of-Function Strategies
Mosaic Analysis
References
Appendix
Where to Find Information on Arabidopsis
Appendix
Critical
Appendix
Cautions
Appendix
Suppliers
Index,
|
| any_adam_object | 1 |
| author | Weigel, Detlef 1961- Glazebrook, Jane |
| author_GND | (DE-588)111425476 |
| author_facet | Weigel, Detlef 1961- Glazebrook, Jane |
| author_role | aut aut |
| author_sort | Weigel, Detlef 1961- |
| author_variant | d w dw j g jg |
| building | Verbundindex |
| bvnumber | BV014109431 |
| callnumber-first | Q - Science |
| callnumber-label | QK495 |
| callnumber-raw | QK495.C9 |
| callnumber-search | QK495.C9 |
| callnumber-sort | QK 3495 C9 |
| callnumber-subject | QK - Botany |
| classification_rvk | WC 4500 WG 4500 |
| classification_tum | BIO 450f CIT 972f |
| ctrlnum | (OCoLC)47989863 (DE-599)BVBBV014109431 |
| dewey-full | 583/.64 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 583 - Eudicots and Ceratophyllales |
| dewey-raw | 583/.64 |
| dewey-search | 583/.64 |
| dewey-sort | 3583 264 |
| dewey-tens | 580 - Plants |
| discipline | Biologie Chemie-Ingenieurwesen Biotechnologie |
| format | Book |
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| id | DE-604.BV014109431 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:57:49Z |
| institution | BVB |
| isbn | 0879695722 0879695730 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009666687 |
| oclc_num | 47989863 |
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| owner_facet | DE-M49 DE-BY-TUM DE-703 DE-20 DE-29T DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | XII, 354 S. in Ringbuch Ill. |
| publishDate | 2002 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Cold Spring Harbor Laboratory Pr. |
| record_format | marc |
| spelling | Weigel, Detlef 1961- Verfasser (DE-588)111425476 aut Arabidopsis a laboratory manual Detlef Weigel ; Jane Glazebrook Cold Spring Harbor, NY Cold Spring Harbor Laboratory Pr. 2002 XII, 354 S. in Ringbuch Ill. txt rdacontent n rdamedia nc rdacarrier Arabidopsis - Aspect moléculaire - Manuels de laboratoire Arabidopsis - Manuels de laboratoire Laboratoriumonderzoek gtt Zandraketten gtt Arabidopsis Laboratory manuals Arabidopsis Molecular aspects Laboratory manuals Arabidopsis cytology Laboratory Manuals Arabidopsis genetics Laboratory Manuals Mutation Laboratory Manuals Plants, Genetically Modified genetics Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf Genetik (DE-588)4071711-2 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Schmalwand Arabidopsis (DE-588)4179774-7 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Pflanzenbau (DE-588)4045547-6 gnd rswk-swf Schmalwand Arabidopsis (DE-588)4179774-7 s Pflanzenbau (DE-588)4045547-6 s DE-604 Gentechnologie (DE-588)4071722-7 s Ackerschmalwand (DE-588)4141299-0 s Genetik (DE-588)4071711-2 s Methode (DE-588)4038971-6 s DE-188 Glazebrook, Jane Verfasser aut Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009666687&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Weigel, Detlef 1961- Glazebrook, Jane Arabidopsis a laboratory manual Arabidopsis - Aspect moléculaire - Manuels de laboratoire Arabidopsis - Manuels de laboratoire Laboratoriumonderzoek gtt Zandraketten gtt Arabidopsis Laboratory manuals Arabidopsis Molecular aspects Laboratory manuals Arabidopsis cytology Laboratory Manuals Arabidopsis genetics Laboratory Manuals Mutation Laboratory Manuals Plants, Genetically Modified genetics Laboratory Manuals Methode (DE-588)4038971-6 gnd Genetik (DE-588)4071711-2 gnd Ackerschmalwand (DE-588)4141299-0 gnd Schmalwand Arabidopsis (DE-588)4179774-7 gnd Gentechnologie (DE-588)4071722-7 gnd Pflanzenbau (DE-588)4045547-6 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4071711-2 (DE-588)4141299-0 (DE-588)4179774-7 (DE-588)4071722-7 (DE-588)4045547-6 |
| title | Arabidopsis a laboratory manual |
| title_auth | Arabidopsis a laboratory manual |
| title_exact_search | Arabidopsis a laboratory manual |
| title_full | Arabidopsis a laboratory manual Detlef Weigel ; Jane Glazebrook |
| title_fullStr | Arabidopsis a laboratory manual Detlef Weigel ; Jane Glazebrook |
| title_full_unstemmed | Arabidopsis a laboratory manual Detlef Weigel ; Jane Glazebrook |
| title_short | Arabidopsis |
| title_sort | arabidopsis a laboratory manual |
| title_sub | a laboratory manual |
| topic | Arabidopsis - Aspect moléculaire - Manuels de laboratoire Arabidopsis - Manuels de laboratoire Laboratoriumonderzoek gtt Zandraketten gtt Arabidopsis Laboratory manuals Arabidopsis Molecular aspects Laboratory manuals Arabidopsis cytology Laboratory Manuals Arabidopsis genetics Laboratory Manuals Mutation Laboratory Manuals Plants, Genetically Modified genetics Laboratory Manuals Methode (DE-588)4038971-6 gnd Genetik (DE-588)4071711-2 gnd Ackerschmalwand (DE-588)4141299-0 gnd Schmalwand Arabidopsis (DE-588)4179774-7 gnd Gentechnologie (DE-588)4071722-7 gnd Pflanzenbau (DE-588)4045547-6 gnd |
| topic_facet | Arabidopsis - Aspect moléculaire - Manuels de laboratoire Arabidopsis - Manuels de laboratoire Laboratoriumonderzoek Zandraketten Arabidopsis Laboratory manuals Arabidopsis Molecular aspects Laboratory manuals Arabidopsis cytology Laboratory Manuals Arabidopsis genetics Laboratory Manuals Mutation Laboratory Manuals Plants, Genetically Modified genetics Laboratory Manuals Methode Genetik Ackerschmalwand Schmalwand Arabidopsis Gentechnologie Pflanzenbau |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009666687&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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