Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2001 |
| Beschreibung: | VIII, 177 S. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes |c von Diana Raffelsbauer |
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| 300 | |a VIII, 177 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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CONTENTS
LIST OF FIGURES U*
LIST OF TABLES VII
1 INTRODUCTION 1
2 RESULTS _ 11
2.1 IDENTIFICATION OF NEW INTEMALIN GENES OF
LISTERIA MONOCYTOGENES EGD . . 12
2.1.1 THE NEW INTEMALIN GENE CLUSTER INLGHE. 12
2.1.2 MOLECULAR CLONING OF INLGHE. 15
2.2 THE GENES INLG AND INLH ARE PRESENT IN MOST L MONOCYTOGENES
SEROTYPES . 16
2.3 SEQUENCE OF THE NEW INTEMALINS INLG, INLH AND INLE. 16
2.4 THE INTEMALIN FAMILY CONSISTS OF HIGHLY CONSERVED PROTEINS. 20
2.5 TRANSCRIPTIONAL STUDIES OF THE GENES INLG, INLH AND INLE. 21
2.6 CONSTRUCTION OF AN IN-FRAME INLGHE DELETION MUTANT. 26
2.7 CHARACTERIZATION OF THE
L. MONOCYTOGENES AINLGHE MUTANT. 26
2.7.1 DELETION OF THE INLGHE GENE CLUSTER REDUCES VIRULENCE. 26
2.7.2 IN VITRO STUDIES USING THE AINLGHE MUTANT. 29
2.8 CONSTRUCTION OF NEW AINLGHE MUTANT STRAINS. 32
2.9 THE L MONOCYTOGENES SINGLE MUTANTS A INLG, AINLH AND A INLE. 34
2.10 THE L MONOCYTOGENES REVERTANT STRAINS INLG+, INLH+ AND INLE+. 36
2.11 THE L MONOCYTOGENES INI COMBINATION MUTANTS. 40
2.12 DELETION OF INLGHE ENHANCES ADHESION TO CACO-2 CELLS. 50
2.13 TRANSCRIPTIONAL STUDIES OF THE GENES INLA, INLB AND INLC BY RT-PCR
. . 52
2.13.1 DELETION OF INLGHE ALTERS EXPRESSION OF THE INLA AND INLB GENES .
52
2.14 TRANSCRIPTIONAL STUDIES OF THE INLGHE GENE CLUSTER BY RT-PCR. 57
2.14.1 DELETION OF SINGLE GENES FROM THE INLGHE CLUSTER DOES NOT INFLU
ENCE TRANSCRIPTION OF THE REMAINING GENES. 57
2.15 TRANSCRIPTION OF THE GENES INLG, INLH AND INLE IS NOT ACTIVATED BY
HEPG-2
CELLS. 61
2.16 IDENTIFICATION OF THE
INLF GENE OF L. MONOCYTOGENES EGD. 63
2.17 THE INLGHE GENE CLUSTER IN L MONOCYTOGENES CLINICAL ISOLATES. 63
2.18 L MONOCYTOGENES CLINICAL ISOLATES AND CELL DEATH. 67
3 DISCUSSION 71
L
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/964268779
11
CONTENTS
4
MATERIAL 87
4.1 BACTERIAL STRAINS AND PLASMIDS . 87
4.2 MEDIA FOR BACTERIAL GROWTH AND ANTIBIOTICS. 88
4.3 MAMMALIAN CELL LINES AND MEDIA FOR CELL CULTURE. 88
4.4 ENZYMES, CHEMICALS, PLASTIC MATERIAL, COMMERCIAL KITS AND
INSTRUMENTS . . 93
4.5 OLIGONUCLEOTIDES.( . 93
5
METHODS
5.1 GENETIC METHODS .
5.1.1 PREPARATION OF DNA .
5.1.2 POLYMERASE CHAIN REACTION (PCR).
5.1.3 GENERAL TECHNIQUES OF MOLECULAR CLONING .
5.2 CONSTRUCTION OF L MONOCYTOGENES INTEMALIN DELETION MUTANTS.
5.2.1 CONSTRUCTION OF INLGHE DELETION MUTANTS.
5.2.2 CONSTRUCTION OF INLG, INLH AND INLE SINGLE MUTANTS AND REVERTANTS
5.3 TRANSCRIPTIONAL STUDIES.
5.3.1 DETERMINATION OF PROMOTER ACTIVITY BASED ON A GFP EXPRESSION
SYSTEM .
5.3.2 ISOLATION OF L. MONOCYTOGENES RNA AND RT-PCRS .
5.4 METHODS FOR BACTERIA AND CELL CULTURE.
5.4.1 MEDIA AND GROWTH CONDITIONS OF BACTERIAL STRAINS.
5.4.2 CELL CULTURE, CELLULAR INVASION AND INTRACELLULAR GROWTH ASSAYS .
. .
5.4.3 QUANTIFICATION OF GFP FLUORESCENCE OF INTRACELLULAR BACTERIA . . .
.
5.4.4 FLUORESCENCE MICROSCOPIC STUDIES.
5.4.5 DETECTION OF APOPTOSIS IN CACO-2 AND J774 CELLS INFECTED WITH L
MONOCYTOGENES.
5.5 OTHER METHODS.
5.5.1 IN VIVO STUDIES .
5.5.2 COMPUTER SEQUENCE ANALYSIS.
99
99
99
100
101
102
102
103
104
104
104
105
105
106
107
107
108
108
108
108
A STRAIN COLLECTION
HI
B NUCLEOTIDE SEQUENCES 115
B.L NUCLEOTIDE SEQUENCE OF THE
INLGHE GENE CLUSTER AND ITS FLANKING GENES . . 115
B.2 NUCLEOTIDE SEQUENCES OF THE MUTANT AND REVERTANT STRAINS.122
B.3 NUCLEOTIDE SEQUENCE OF THE INLF GENE OF L MONOCYTOGENES EGD.150
C SEQUENCE ALIGNMENT OF INTEMALINS 151
D ABBREVIATIONS 155
E PUBLICATIONS 157
F CURRICULUM VITAE 159
G LEBENSLAUF
161 |
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| indexdate | 2024-10-09T18:05:06Z |
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| language | English |
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| physical | VIII, 177 S. Ill., graph. Darst. |
| publishDate | 2001 |
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| spelling | Raffelsbauer, Diana Verfasser aut Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes von Diana Raffelsbauer 2001 VIII, 177 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2001 Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Listeria monocytogenes (DE-588)4203141-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Listeria monocytogenes (DE-588)4203141-2 s Molekulargenetik (DE-588)4039987-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009666004&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Raffelsbauer, Diana Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes Molekulargenetik (DE-588)4039987-4 gnd Listeria monocytogenes (DE-588)4203141-2 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4203141-2 (DE-588)4113937-9 |
| title | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes |
| title_auth | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes |
| title_exact_search | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes |
| title_full | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes von Diana Raffelsbauer |
| title_fullStr | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes von Diana Raffelsbauer |
| title_full_unstemmed | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes von Diana Raffelsbauer |
| title_short | Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes |
| title_sort | identification and characterization of the inlghe gene cluster of listeria monocytogenes |
| topic | Molekulargenetik (DE-588)4039987-4 gnd Listeria monocytogenes (DE-588)4203141-2 gnd |
| topic_facet | Molekulargenetik Listeria monocytogenes Hochschulschrift |
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| work_keys_str_mv | AT raffelsbauerdiana identificationandcharacterizationoftheinlghegeneclusteroflisteriamonocytogenes |